Profiling of Somatic Mutations in Phaeochromocytoma and Paraganglioma by Targeted Next Generation Sequencing Analysis by Andrea Luchetti, Diana Walsh, Fay Rodger, Graeme Clark, Tom Martin, Richard Irving, Mario Sanna, Masahiro Yao, Mercedes Robledo, Hartmut P. H. Neumann, Emma R. Woodward, Farida Latif, Stephen Abbs, Howard Martin, Eamonn R. Maher

“…To further elucidate the role of somatic mutation in PCC/PGL/HNPGL tumourigenesis, we employed a next generation sequencing strategy to analyse “mutation hotspots” in 50 human cancer genes. …”
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Insights from the single-cell level: lineage trajectory and somatic-germline interactions during spermatogenesis in dwarf surfclam Mulinia lateralis by Yajuan Li, Huilan Wei, Xiaoting Dai, Lijing Zhang, Liangjie Liu, Xiaomei Chen, Tian Liu, Ya Shu, Yaxin Yang, Shi Wang, Zhenmin Bao, Lingling Zhang

“…Results In this study, over 4,600 testicular cells from various samples were employed to identify single-cell heterogeneity on a more comprehensive scale. …”
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Dense integration of chlorocatechols crosslinked polyphenylene sulfide solid-state separator for Li metal-free batteries by Haitao Zhou, Lianlu Wan, Jiacheng Han, Jian-Chun Wu, Yihong Deng, Jie Gu, Huanggang Wang, Hongquan Gao, Zilong Su

“…In this study, we propose a hydrothermal process to introduce a chlorocatechol-based cross-linker onto the surface of highly crystalline polyphenylene sulfide (PPS) powder. By employing the dry electrode process, a PPS-based solid-state separator (PPS-SSS) is fabricated, featuring a thin profile (18±2 μm), a smoother surface, and a denser structure, significantly enhancing its mechanical properties. …”
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‘I am happy to be listened to’: co-creation of a simple tool to measure women’s experiences of respectful maternity care in urban Tanzania by Brenda Sequeira D’mello, Natasha Housseine, Hussein Lesio Kidanto, Nanna Maaløe, Jos van Roosmalen, Dan Wolf Meyrowitsch, Thomas van den Akker, Zainab Muniro, Evance Polin, Nuswe Ambokile, Charles Festo, Jane Brandt Sørensen, David Sando

“…We aimed to (i) co-create a routine RMC measurement tool (RMC-T) for congested maternity units in Dar es Salaam, Tanzania, and (ii) assess the RMC-T’s acceptability among women and healthcare stakeholders. Method We employed a participatory approach utilizing multiple mixed methods. …”
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Cetacean feeding modelling using machine learning: A case study of the Central-Eastern Mediterranean Sea by Carla Cherubini, Giulia Cipriano, Leonardo Saccotelli, Giovanni Dimauro, Giovanni Coppini, Roberto Carlucci, Carmelo Fanizza, Rosalia Maglietta

“…Multiple subsets of environmental predictors—physiographic, physical, inorganic, and bio-chemical—were employed to develop and evaluate ML models tailored to feeding prediction. …”
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Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease by S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina

“…The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research. …”
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Announcement effect of tender offer share buyback around turmoil period – evidence from India by Suresha B., Kavitha Desai, Rejoice Thomas, Nijumon K John, Elizabeth Renju Koshy

“…The event study methodology using a market model was employed to determine the presence of abnormal returns during the event period, which consisted of –21 days and +21 days. …”
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Atmospheric Corrosion, Antibacterial Properties, and Toxicity of Silver Nanoparticles Synthesized by Two Different Routes by I. DeAlba-Montero, Claudio A. Ruiz-Torres, Diana P. Portales-Pérez, Fidel Martínez-Gutierrez, Félix Echeverría, Martha E. Compeán-Jasso, Yolanda G. Cataño-Cañizales, Facundo Ruiz

“…Silver nanoparticles (AgNPs) have been widely employed or incorporated into different materials in biological application, due to their antibacterial properties. …”
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Effect of Laryngeal Mask Airway Insertion on Intraocular Pressure Response: Systematic Review and Meta-Analysis by Mohammed Suleiman Obsa, Zewde Zema kanche, Robera Olana Fite, Tilahun Saol Tura, Bulcha Guye Adema, Aseb Arba Kinfe, Melkamu Worku kercho, Kebreab Paulos chanko, Getahun Molla Shanka, Atkuregn Alemayehu Lencha, Gedion Asnake Azeze, Lolemo Kelbiso Hanfore, Nefsu Awoke Adulo, Blen Kassahun Dessu, Getahun Dendir Wolde, Shimelash Bitew Workie

“…A random-effect model was employed to estimate the pooled standard mean differences due to severe heterogeneity (I2 79.45,  p ≤ 0.001). …”
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Thermal assessment of Li-ion battery cells and coolant in hybrid electric vehicles system: Application of conjugate condition and response surface methodology by Asif Afzal, M.K. Ramis, R.D. Jilte, Mamdooh Alwetaishi, Sung Goon Park, Abdulrajak Buradi, Qasem M. Al-Mdallal, Ümit Ağbulut

“…The RS methodology is employed to analyze the MT of the battery. The MT difference obtained with increasing S‾q and Re is quite significant. …”
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Polymorphisms in the leptin gene and their role in adolescent obesity and metabolic health by Raden Ayu Tanzila, Irfannuddin Irfannuddin, Yudianita Kesuma, Iche Andriyani Liberty, Miranti Dwi Hartanti, Ni Made Elva Mayasari, Wieke Anggraini, Liza Chairani, Thia Prameswarie, Indri Ramayanti, Wulan Aristi, Delvi Larasari

“…Standard laboratory protocols for serum lipid measurements, deoxyribonucleic acid (DNA) extraction, allele analysis by polymerase chain reaction (PCR), and LEP and insulin analysis by enzyme-linked immunosorbent assay (ELISA) were employed. The study revealed the allelic frequencies for gene LEP1: non-obese (G = 0.45, A = 0.55) and obese (G = 0.87, A = 0.13), and gene LEP2: non-obese (G = 0.54, A = 0.45) and obese (G = 0.67, A = 0.33). …”
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The triglyceride–glucose index and its obesity-related derivatives as predictors of all-cause and cardiovascular mortality in hypertensive patients: insights from NHANES data with... by Chenyang Li, Zixi Zhang, Xiaoqin Luo, Yichao Xiao, Tao Tu, Chan Liu, Qiming Liu, Cancan Wang, Yongguo Dai, Zeying Zhang, Cheng Zheng, Jiafeng Lin

“…Cox proportional hazards models and restricted cubic splines were employed to explore mortality risk and potential nonlinear relationships. …”
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An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing by Jacob W. Fleming, Molly C. McCloskey, Kevin Gray, David R. Nash, Vincent Leung, Christos Michas, Shawn M. Luttrell, Christopher Cavanaugh, Julie Mathieu, Shawn Mcquire, Mark Bothwell, David L. Mack, Nicholas A. Geisse, Alec S.T. Smith

“…We present a turnkey iPSC-based model of the NMJ employing channelrhodopsin-2 expression within the motor neuron (MN) population driving muscle contraction in response to blue light. …”
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Cardiovascular and renal safety outcomes of hypoxia-inducible factor prolyl-hydroxylase inhibitor roxadustat for anemia patients with chronic kidney disease: a systematic review an... by Lei Tian, Mengdi Wang, Mengchao Liu, Yanyu Pang, Jingwen Zhao, Bingjie Zheng, Yutong Wang, Wenjing Zhao

“…This systematic review and meta-analysis were conducted to evaluate the cardiac and kidney-related adverse effects of roxadustat for the treatment of anemia in CKD patients. 18 trials with a total of 8806 participants were identified for analysis. We employed a fixed-effects model for analysis. The pooled result revealed no significant difference in the risk of occurrence of cardiac disorders when comparing CKD patients receiving roxadustat with the placebo (RR = 1.049; CI [0.918 to 1.200]) or ESA (RR = 1.066; CI [0.919 to 1.235]), in both dialysis-dependent (DD) (RR = 1.094; CI [0.925 to 1.293]) or non-dialysis-dependent (NDD) (RR = 1.036; CI [0.916 to 1.171]) CKD patients. …”
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Diabetes and Cataracts Development—Characteristics, Subtypes and Predictive Modeling Using Machine Learning in Romanian Patients: A Cross-Sectional Study by Adriana Ivanescu, Simona Popescu, Adina Braha, Bogdan Timar, Teodora Sorescu, Sandra Lazar, Romulus Timar, Laura Gaita

“…A Random Forest Classification algorithm was employed to predict the incidence of different associations of cataracts (1, 2, or 3 types). …”
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The atherogenic index of plasma is associated with an increased risk of diabetes in non-obese adults: a cohort study by Jun Cao, Zhaohai Su, Jiangyong Yang, Bilong Zhang, Rengui Jiang, Weiling Lu, Zhenhua Huang, Zhenhua Huang, Zheng Xie

“…We used Cox proportional hazards regression to assess the relationship between baseline AIP levels and diabetes incidence. We also employed cubic spline functions and smooth curve fitting to investigate potential nonlinear relationships. …”
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Improving community-based first response to out of hospital cardiac arrest (FirstCPR): protocol for a cluster randomised controlled trial by Sandra Ware, Blake Angell, Julie Redfern, Clara Chow, Cate Ferry, Lee Taylor, Christopher Semsarian, Adrian Bauman, Janet E Bray, Simone Marschner, Michael Nelson, Saurabh Kumar, Matthew Vukasovic, Kevin Lai, Paul M Middleton, Pramesh Kovoor, Garry Jennings, Sonali Munot, Andrew Coggins, Alan Robert Denniss, Sarah Khanlari, Ian Opperman

“…This study aims to assess the effectiveness of a community organisation targeted multicomponent education and training initiative on being willing to respond to OHCAs. The study employs a novel approach to reaching community members via social and cultural groups, and the intervention aims to address commonly cited barriers to training including lack of availability, time and costs.Methods and analysis FirstCPR is a cluster randomised trial that will be conducted across 200 community groups in urban and regional Australia. …”
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Short-Term Outcomes of Endoscopic Ultrasound-Guided Pancreatic Cyst Ablation: A Systematic Review and Meta-Analysis by Ahmed Al Qady, Kapil Dev Nayar, Fatima Elmustafa, Mohamed Salih, Joseph Emran, Amir Beirat, Sasmith Menakuru, Dana Harris, Dan J. Echols, Baoan Ji, John M. DeWitt, Zhen Wang, Fernando F. Stancampiano, Yan Bi

“…The effectiveness of this treatment is further enhanced when chemoablative agents are employed.…”
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Ethanol extract of Polygonatum cyrtonema Hua mitigates non-alcoholic steatohepatitis in mice by Dongliang Chen, Yue Shen, Yue Shen, Fang Huang, Bo Huang, Shangfu Xu, Shangfu Xu, Lisheng Li, Lisheng Li, Jie Liu, Zheng Li, Xia Li

“…One hand, liver injury was assessed by serum enzymes, biochemistry, and histopathology; On the other hand, RNA-seq, qPCR, and Western blot were employed to investigate the related molecular mechanisms.Results211 metabolites were identified through UHPLC-MS/MS analysis. …”
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Chronic Inhibition of FAAH Reduces Depressive-Like Behavior and Improves Dentate Gyrus Proliferation after Chronic Unpredictable Stress Exposure by A. R. Tejeda-Martínez, J. M. Viveros-Paredes, G. V. Hidalgo-Franco, E. Pardo-González, V. Chaparro-Huerta, R. E. González-Castañeda, M. E. Flores-Soto

“…Most of these auspicious responses to enzyme inhibitor administration were blocked by employing a cannabinoid receptor antagonist. In conclusion, the chronic inhibition of FAAH generated an antidepressant effect, promoting neural progenitor proliferation and BDNF expression, while reducing adrenal gland weight and oxidative stress in mice under the CUS model.…”
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