Comparison of the adequacies of the OSCE and vOSCE to assess the competencies required under Brazilian medical curriculum guidelines: a multicenter study by Hervaldo Sampaio Carvalho, Maria do Carmo Barros Melo, Fabiana Maria Kakehasi, Priscila Menezes Ferri Liu, Fernanda Gontijo Minafra Silveira Santos, Ricardo Luiz de Melo Martins, Marilene Vale de Castro Monteiro, Marcelo Esteves Chaves Campos, Roberto Zonato Esteves, Matheus Belloni Torsani, Iolanda de Fátima Lopes Calvo Tiberio, Akihito Inca Atahualpa Urdiales, Andrea Mora De Marco Novellino, Rosiane Viana Zuza Diniz, Helena Ferreira Moura, Maria Alice de Vilhena Toledo, Célia Regina Machado Saldanha, Andrea Pedrosa Ribeiro Alves Oliveira, Patricia Zen Tempski, Itamar de Souza Santos, Milton de Arruda Martins

“…Conclusion The vOSCE can be employed to assess relevant competencies. However, it faces limitations regarding 8% (8/100) of the items, and some items (3%) cannot be assessed via either method. …”
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Artificial intelligence analysis of thermal energy for convectively heated ternary nanofluid flow in radiated channel considering viscous dissipations aspects by Hamid Qureshi, Amjad Ali Pasha, Muhammad Asif Zahoor Raja, Zahoor Shah, Salem Algarni, Talal Alqahtani, Waqar Azeem Khan, Moinul Haq

“…Advance computational technique with combination of AI with Python, Mathematica and Matlab (AIPMM) employing Levenberg Marquardt Neural Network Algorithm (LMNNA), is used for solving a transformed system of ODEs, which was obtained from the system of PDEs of present model. …”
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Association between exposure to ambient air pollutants and metabolic syndrome in the vicinity of the Taklamakan Desert by Zhe Wang, Weidong Ji, Yushan Wang, Lin Li, Kai Wang, Hongze Liu, Yining Yang, Yi Zhou

“…Spatio-temporal data from satellite observations were employed to estimate ambient pollution levels, encompassing particulate matter with diameters of up to 1.0 µm (PM1), 2.5 µm (PM2.5), and 10 µm (PM10), along with Ozone (O3) and Carbon monoxide (CO). …”
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Beta-Blockers After PCI for Stable Coronary Artery Disease and Preserved Left Ventricular Ejection Fraction by Safi U. Khan, MD, MS, Usman Ali Akbar, MD, Muhammad Shahzeb Khan, MD, Kershaw V. Patel, MD, Amna Nadeem, MBBS, Samarth Thakkar, MD, Hassaan B. Arshad, MD, Salim S. Virani, MD, PhD, Khurram Nasir, MD, MPH, Sachin S. Goel, MD, Alpesh R. Shah, MD, William Zoghbi, MD, Neal S. Kleiman, MD

“…Methods: This retrospective cohort study employed target trial emulation and incident user design, utilizing the TriNetx database (2009-2024). …”
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Bulk and single-cell transcriptome revealed the metabolic heterogeneity in human glioma by Yong Xiao, Mengjie Zhao, Ran Wang, Liang Liu, Chong Xiang, Taiping Li, Chunfa Qian, Hong Xiao, Hongyi Liu, Yuanjie Zou, Xianglong Tang, Kun Yang

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were employed to elucidate the biological functions of genes of interest. …”
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Identification of standing dead trees in Robinia pseudoacacia plantations across China’s Loess Plateau using multiple deep learning models by Li Zhang, Xiaodong Gao, Shuyi Zhou, Zhibo Zhang, Tianjie Zhao, Yaohui Cai, Xining Zhao

“…Subsequently, the model was employed to quantify the spatial distribution of SDTs across the CLP by estimating the number of dead trees per unit area. …”
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EV Hybrid Battery With Integrated Multilevel Neutral-Point-Clamped Interfacing and Lossless Intermodule State-of-Charge Balancing by Gabriel Garcia-Rojas, Sergio Busquets-Monge, Alber Filba-Martinez, Turev Sarikurt, Salvador Alepuz, Josep Bordonau

“…Instead, hybrid batteries, combining only two battery cell chemistries, with distinct particular strengths, such as high specific energy or high specific power, offer an opportunity to cover a wide range of vehicle battery specifications while avoiding oversizing and dispersion in the cells to be employed. This work introduces a novel hybrid battery configuration, where the interfacing between the two sets of cells is accomplished through a bidirectional multilevel neutral-point-clamped dc–dc converter. …”
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Electrochemotherapy with bleomycin, oxaliplatin, or cisplatin in mouse tumor models, from tumor ablation to in situ vaccination by Katja Uršič Valentinuzzi, Katja Uršič Valentinuzzi, Urška Kamenšek, Urška Kamenšek, Simona Kranjc Brezar, Chloe Heranney, Chloe Heranney, Tilen Komel, Tilen Komel, Simon Buček, Simon Buček, Maja Čemažar, Maja Čemažar, Gregor Serša, Gregor Serša

“…By evaluating ECT’s potential for in situ vaccination, our goal was to pave the way for future advancements for its combination with emerging (immuno)therapies, leading to enhanced responses and outcomes.MethodsWe employed two mouse tumor models, the immunologically cold B16F10 melanoma and 4T1 mammary carcinoma, to explore both local and systemic (i.e., abscopal) antitumor effects following equieffective intratumoral ECT with BLM, OXA, and CDDP. …”
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Clinicopathological Significance of FOXO4 Expression and Correlation with Prx1 in Head and Neck Squamous Cell Carcinoma by Yunping Lu, Yajun Shen, Lingyu Li, Min Zhang, Min Wang, Lihua Ge, Jing Yang, Xiaofei Tang

“…FOXO4 expression is negatively related to Prx1 significantly in HNSCC tissues. By employing a 4NQO-induced oral carcinogenesis mouse model, we confirmed that FOXO4 expression was reduced in 4NQO-induced squamous cell carcinoma (SCC) tongue tissues compared with those in normal tissues. …”
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The quality of Tibetan sheep meat from pastures was synergistically regulated by the rumen microbiota and related genes at different phenological stages by Xiaowei Chen, Yuzhu Sha, Xiu Liu, Yanyu He, Wenhao Li, Liangwei Yao, Jiqing Wang, Wenxin Yang, Qianling Chen, Min Gao, Wei Huang, Bin Ma, Bin Ma

“…In this study, RT-qPCR and 16S omics were employed to assess meat quality and intestinal microbiota. …”
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Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2 by Zhilin Sui, Xianxian Wu, Jiaxin Wang, ShihJye Tan, Chao Zhao, Zhentao Yu, Chuanyue Wu, Xiaoxiao Wang, Ling Guo

“…To overcome this limitation, we have created an in vitro system that employs mesenchymal stromal cells (MSCs) or fibroblasts to serve as a supportive scaffold for lung cancer cells that do not form organoids. …”
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Helicobacter pylori SlyD stabilizes TPT1 via hnRNPK and enhances OCT1-mediated CDX2 transcriptional activation to drive gastric intestinal metaplasia by Shuwen Zheng, Yingying Wang, Chuxuan Ni, Rui Guo, Xunan Qiu, Jijun Chen, Lu Wang, Xiaohu Sun, Moye Chen, Yunen Liu, Yuan Yuan, Yuehua Gong

“…Real-time qPCR and western blot were subsequently used to assess the expression levels of hnRNPK, TPT1, OCT1, and GIM markers. RNA sequencing was employed to identify differentially expressed genes associated with H. pylori SlyD infection. …”
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Cutting-edge Bioinformatics strategies for synthesizing Cyclotriazadisulfonamide (CADA) analogs in next-Generation HIV therapies by Jay Gabriel B. Larga, Wrynan T. Munabirul, Abu Tayab Moin, Md Maisum Sarwar Jyoti, Mst Sanjida Nasrin, Minhaz Abdullah Al Mueid, Abdul Ahad, Anwar Parvez, Mst Sabrina Yeasmin, Rupali M. Barhate, Rajesh B. Patil, Margel C. Bonifacio

“…AutoDock Vina version 1.2.4 was employed to predict the binding energies of these analogs. …”
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Comprehensive analysis of metabolomics and transcriptomics reveals varied tepal pigmentation across Gloriosa varieties by Yue Sun, Pinli Hu, Yanan Jiang, Zhenzhen Wang, Jiaxing Chang, Yiwei Zhou, Haojing Shao

“…Abstract Gloriosa L. possesses exceptional ornamental value, with its floral hues exhibiting a wide range of variations. In this study, we employed sophisticated colorimetry, Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS), and transcriptome sequencing to investigate the phenotypic expression of tepal colors, the composition of carotenoids and anthocyanins, and the differential gene expression in four Gloriosa varieties during their full bloom phase. …”
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Protein kinases MpkA and SepH transduce crosstalk between CWI and SIN pathways to activate protective hyphal septation under echinocandin cell wall stress by Alexander G. Doan, Jessica E. Schafer, Casey M. Douglas, Matthew S. Quintanilla, Meredith E. Morse, Harley Edwards, Walker D. Huso, Kelsey J. Gray, JungHun Lee, Joshua K. Dayie, Steven D. Harris, Mark R. Marten

“…The echinocandin micafungin, an inhibitor of β-(1,3)-glucan synthase, was employed to induce this stress. We observed a strong positive correlation between micafungin treatment and septum density in wild-type strains. …”
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Modulation of DAPK1 expression by its alternative splice variant DAPK1-215 in cancer by QingShui Wang, ShuYun Weng, WenTing Zhong, YouYu Lin, Yan Yu, YiMin Huang, LiLin Ge, XiuLi Zhang, FangQin Xue, Yehuda G. Assaraf, Yao Lin

“…Cell migration, invasion, and proliferation assays were conducted in vitro, and a zebrafish model was employed to evaluate metastatic potential. RNA pull-down and CLIP-seq analyses were performed to identify potential RNA-binding proteins. …”
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The steps that young people and suicide prevention professionals think the social media industry and policymakers should take to improve online safety. A nested cross-sectional stu... by Jo Robinson, Jo Robinson, Pinar Thorn, Pinar Thorn, Samuel McKay, Samuel McKay, Hannah Richards, Hannah Richards, Rikki Battersby-Coulter, Rikki Battersby-Coulter, Michelle Lamblin, Michelle Lamblin, Laura Hemming, Laura Hemming, Laura Hemming, Louise La Sala, Louise La Sala

“…The proportion of participants “strongly agreeing”, “somewhat agreeing”, “neither agreeing nor disagreeing”, and “somewhat disagreeing” or “strongly disagreeing” for each item were calculated; items that achieved =>80% of agreement from both panels were strongly endorsed.ResultsThere was limited consensus across the two groups regarding the utility of the safety strategies currently employed by companies. However, both groups largely agreed that self-harm and suicide-related content should be restricted. …”
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Targeted ErbB4 receptor activation prevents D-galactose-induced neuronal senescence via inhibiting ferroptosis pathway by Ji-Ji Dao, Ji-Ji Dao, Wei Zhang, Wei Zhang, Chong Liu, Chong Liu, Qian Li, Qian Li, Chen-Meng Qiao, Chen-Meng Qiao, Chun Cui, Chun Cui, Yan-Qin Shen, Yan-Qin Shen, Shuang-Xi Chen, Shuang-Xi Chen, Wei-Jiang Zhao, Wei-Jiang Zhao

“…Immunofluorescence staining, western blot, and biochemical assays were primarily employed to assess changes in both senescence- and ferroptosis-related molecules in mouse hippocampal tissues in response to each treatment. …”
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Assessing and predicting the value of ecosystem services in Sanya City, Hainan Island, China by Peihong Song, Qiu Yang, Wenyin Wu, Tianyan Su, Yamin Jiang, Jingli Lu, Zhongyi Sun, Jie Zhang, Rui Yu, Peng Wang, Lan Wu, Huai Yang, Wenjie Liu

“…Additionally, the Future land-use simulation (FLUS) model was employed to predict land-use changes in 2030 under five different scenarios. …”
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Clinical and cost-effectiveness of Knee Arthroplasty versus Joint Distraction for Osteoarthritis (KARDS): protocol for a multicentre, phase III, randomised control trial by Deborah D Stocken, Dennis G McGonagle, David Murray, Catherine Reynolds, Catherine Fernandez, David R Ellard, Andrew Metcalfe, Hemant Sharma, Paul Harwood, Hamish Simpson, Hemant Pandit, Susanne Arnold, Cerys Joyce Tassinari, Ruchi Higham, Isabelle Louise Smith, Ruben Mujica-Mota, Thomas William Hamilton, Julie Croft

“…The KARDS trial will investigate whether KJD is non-inferior to KR in terms of patient-reported postoperative pain 12 months post-surgery.Methods and analysis KARDS is a phase III, multicentre, pragmatic, open-label, individually randomised controlled non-inferiority trial comparing KJD with KR in patients with severe knee OA, employing a hybrid-expertise design, with internal pilot phase and process evaluation. 344 participants will be randomised (1:1) to KJD or KR. …”
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