Cultivating crayfish (Procambarus clarkii) significantly enhances the quantity and diversity of soil microorganisms: evidence from the comparison of rice-wheat and rice-crayfish ro... by Hui Xu, Hui Xu, Dan Wang, Dan Wang, Dan Wang, Xuguang Li, Xuguang Li, Jiajia Li, Jiajia Li, Yu Xu, Yu Xu, Zhiqiang Xu, Zhiqiang Xu

“…Random forest prediction analysis was employed to identify potential biomarkers to distinguish the two microbial communities. …”
Get full text

Eco-friendly alginate/PCL-TiO2 hybrid biocomposites: Preparation, properties, and methylene blue photodegradation by Bunga Fisikanta Bukit, Agus Wedi Pratama, Erna Frida, Bakti Berlyanto Sedayu, Dina Fransiska, Deni Purnomo, Emma Rochima, Istiqomah Rahmawati, Sona Suhartana, Firda Aulya Syamani

“…Various characterization techniques, including XRD, FT-IR, UTM, DSC, TGA, biodegradation testing, and methylene blue photodegradation, were employed to evaluate the properties of the composites. …”
Get full text

A Native LH1–RC–HiPIP Supercomplex from an Extremophilic Phototroph by Kazutoshi Tani, Ryo Kanno, Kenji V. P. Nagashima, Mai Kawakami, Naho Hiwatashi, Kazuna Nakata, Sakiko Nagashima, Kazuhito Inoue, Shinichi Takaichi, Endang R. Purba, Malgorzata Hall, Long-Jiang Yu, Michael T. Madigan, Akira Mizoguchi, Bruno M. Humbel, Yukihiro Kimura, Zheng-Yu Wang-Otomo

“…Abstract Halorhodospira (Hlr.) halophila strain BN9622 is an extremely halophilic and alkaliphilic purple phototrophic bacterium and has been widely used as a model for exploring the osmoadaptive and photosynthetic strategies employed by phototrophic extreme halophiles that enable them to thrive in hypersaline environments. …”
Get full text

Computer-guided identification of novel inhibitors of apoptosis-signaling kinase 1 from Spondia mombim bioactive compounds against colorectal cancer by Olusola Olalekan Elekofehinti, Hannah Oluwaseun Popoola, Adedotun Olayemi Oluwatuyi, Opeyemi Iwaloye, Moses Orimoloye Akinjiyan, Oluwamodupe Cecilia, Frank Abimbola Ogundolie, Olalekan Isaac Olatunde, Oussama Abchir, Samir Chtita, Joao Batista Texeira Rocha

“…Method In this study, computational models such as flexible docking, induced fit docking, and binding free energy calculation were employed to identify small molecule inhibitors from known bioactive compounds of Spondias mombin in reference to 5-fluorouracil (Colorectal cancer standard drug) targeting apoptosis-signaling kinase 1. …”
Get full text

T-cell receptor beta variable gene polymorphism predicts immune-related adverse events during checkpoint blockade immunotherapy by Linghua Wang, Siqing Fu, Aung Naing, Funda Meric-Bernstam, Carl Morrison, Joud Hajjar, Mingxuan Xu, Anas Alshawa, Bettzy Stephen, Ying Yuan, Jordi Rodon Ahnert, Abdulrazzak Zarifa, Shrutii Sarda, Timothy Looney, Sapna P Patel, Geoffrey M Lowman, Dzifa Yawa Duose, Jeffrey M Conroy, Evan Kwiatkowski

“…However, the repetitive nature of the TCRB locus and incomplete genome assembly has hampered the evaluation of TRBV polymorphisms in the past.Patients and methods We used a novel method for long-amplicon next generation sequencing of rearranged TCRB chains from peripheral blood total RNA to evaluate the link between TRBV polymorphisms and irAEs in patients treated with immunotherapy for cancer. We employed multiplex PCR to create amplicons spanning the three beta chain complementarity-determining regions (CDR) regions to enable detection of polymorphism within the germline-encoded framework and CDR1 and CDR2 regions in addition to CDR3 profiling. …”
Get full text

Agricultural GDP exposure to drought and its machine learning-based prediction in the Jialing River Basin, China by Xinzhi Wang, Qingxia Lin, Zhiyong Wu, Yuliang Zhang, Changwen Li, Ji Liu, Shinan Zhang, Songyu Li

“…Finally, four machine learning methods (Bayesian, BiGRU, CLA, and MLP) were employed to predict hydrometeorological variables from 2021 to 2030, and the agricultural economic exposures to drought under five shared socioeconomic pathways (SSPs) were also predicted. …”
Get full text

Modeling the determinants of attrition in a two-stage epilepsy prevalence survey in Nairobi using machine learning by Daniel M. Mwanga, Isaac C. Kipchirchir, George O. Muhua, Charles R. Newton, Damazo T. Kadengye, Abankwah Junior, Albert Akpalu, Arjune Sen, Bruno Mmbando, Charles R. Newton, Cynthia Sottie, Dan Bhwana, Daniel Mtai Mwanga, Damazo T. Kadengye, Daniel Nana Yaw, David McDaid, Dorcas Muli, Emmanuel Darkwa, Frederick Murunga Wekesah, Gershim Asiki, Gergana Manolova, Guillaume Pages, Helen Cross, Henrika Kimambo, Isolide S. Massawe, Josemir W. Sander, Mary Bitta, Mercy Atieno, Neerja Chowdhary, Patrick Adjei, Peter O. Otieno, Ryan Wagner, Richard Walker, Sabina Asiamah, Samuel Iddi, Simone Grassi, Sloan Mahone, Sonia Vallentin, Stella Waruingi, Symon Kariuki, Tarun Dua, Thomas Kwasa, Timothy Denison, Tony Godi, Vivian Mushi, William Matuja

“…Important predictors of attrition included proximity to industrial areas, male gender, employment, education, smaller households, and a history of complex partial seizures. …”
Get full text

Exploring the shared gene signatures and mechanism among three autoimmune diseases by bulk RNA sequencing integrated with single-cell RNA sequencing analysis by Xiaofang Liu, Bin Li, Yuxi Lin, Xueying Ma, Yingying Liu, Lili Ma, Xiaomeng Ma, Xia Wang, Nanjing Li, Xiaoyun Liu, Xiaohong Chen

“…Weighted Gene Co-Expression Network Analysis (WGCNA) was employed to construct gene co-expression networks and identify disease-associated modules. …”
Get full text

A dextrorotatory residues-incorporated bioactive dodecapeptide against enterohemorrhagic Escherichia coli by Ping Zeng, Xuemei Yang, Kwok-Yin Wong, Sheng Chen, Kin-Fai Chan, Sharon Shui Yee Leung, Lanhua Yi

“…Finally, the potential application of zp39 in controlling EHEC in food was tested with spinach juice and the Galleria mellonella larvae model was employed to assess the in vivo efficacy. Results: Peptide zp39 presented an amphiphilic helical structure. …”
Get full text

Association between clinical-biological characteristics of Klebsiella pneumoniae and 28-day mortality in patients with bloodstream infection by Xiaofeng Yu, Yi Zhao, Xiao Sun, Jiahui Luan, Haiyan Wang, Tianyu Sun, Tongtong Lin, Xia Zhou, Wei Yang, Ziguang Deng, Bo Liu, Hongyun Cao

“…Logistic regression modeling was employed to evaluate the risk factors associated with 28-day mortality in patients with KP BSI, considering both host characteristics and the characteristics of the causative Klebsiella pneumoniae (KP) isolates. …”
Get full text

Lymphatic leaks – success of intranodal lymphangiogram first strategy by Alan Campbell, Diana Velazquez-Pimentel, Matthew Seager, Richard Hesketh, Julian Hague, Jowad Raja, Jocelyn Brookes, An Ngo, Miles Walkden, Anthie Papadopoulou, Daron Smith, Borzoueh Mohammadi, Ravi Barod, Mohammed Rashid Akhtar, Jimmy Kyaw Tun, Deborah Elise Low, Ian Daniel Renfrew, Tim Fotheringham, Conrad von Stempel

“…Conclusions ILAG can be employed a first line interventional therapeutic technique to treat clinically significant lymphatic leaks that are refractory to conservative and medical management. …”
Get full text

Comparative impact of tertiary lymphoid structures and tumor-infiltrating lymphocytes in cholangiocarcinoma by Chun-Nan Yeh, Yu-Chan Chang, Yi-Chen Yeh, Yu-Chao Wang, Dennis Shin-Shian Hsu, Meng-Lun Lu, Michael Hsiao, Ming-Huang Chen, Nai-Jung Chiang, Shin-Yi Chung, Chien-Jung Huang, Ming-Hsien Chan, Tzu-Sheng Hsu, Yi-Ping Hung

“…Immunohistochemistry and RNA sequencing were employed to assess TLSs and TILs presence and activity. …”
Get full text

Blockchain-assisted improved interval type-2 fuzzy deep learning-based attack detection on internet of things driven consumer electronics by Rana Alabdan, Bayan Alabduallah, Nuha Alruwais, Munya A. Arasi, Somia A. Asklany, Omar Alghushairy, Fouad Shoie Alallah, Abdulrhman Alshareef

“…The ICOA-IT2FDL technique employs a linear scaling normalization (LSN) approach for data normalization. …”
Get full text

Enhancing the ductility of cast Mg-Li alloys via dispersed α-Mg phase mitigating the dimension and distribution of interspersed eutectics along grain boundaries by Yu Wang, Ziyang Xia, Jingpeng Xiong, Gang Zeng, Penghao Wang, Lan Luo, Ruizhi Wu, Jian Wang, Yong Liu

“…In-situ stretching techniques were employed to scrutinize the microstructural evolution of alloys during tensile deformation, elucidating that the deformation compatibility of alloys correlates with the average size of the I-phase and their distribution along GBs and PBs. …”
Get full text

Staged angioplasty using a full-length balloon catheter to achieve maturation of arteriovenous fistulas by Chung Won Lee, Chang Ho Jeon, Sung Woon Chung, Chung Won Lee, Up Huh, Jongwon Kim, Hyuncheol Jeong

“…To minimize balloon overlap and the risk of barotrauma to the immature vein, serial-staged upsizing balloon angioplasty with a long balloon catheter covering the entire vein segment was employed approximately 2 weeks apart. RESULTS: Twenty-three patients (mean age, 69.50 years; mean follow-up, 620.62 days) with average diameters of the radial artery and cephalic vein at 2.14 ± 0.5 mm and 2.43 ± 0.5 mm, respectively, were enrolled. …”
Get full text

Spatial heterogeneity of soil factors enhances intraspecific variation in plant functional traits in a desert ecosystem by Yong-chang Wang, Yong-chang Wang, Yong-chang Wang, Xue-ni Zhang, Xue-ni Zhang, Xue-ni Zhang, Ji-fen Yang, Ji-fen Yang, Ji-fen Yang, Jing-ye Tian, Jing-ye Tian, Jing-ye Tian, Dan-hong Song, Dan-hong Song, Dan-hong Song, Xiao-hui Li, Xiao-hui Li, Xiao-hui Li, Shuang-fu Zhou, Shuang-fu Zhou, Shuang-fu Zhou

“…IntroductionFunctional traits of desert plants exhibit remarkable responsiveness, adaptability and plasticity to environmental heterogeneity.MethodsIn this study, we measured six crucial plant functional traits (leaf carbon, leaf nitrogen, leaf phosphorus, leaf thickness, chlorophyll concentration, and plant height) and employed exemplar analysis to elucidate the effects of soil environmental heterogeneity on intraspecific traits variation in the high-moisture-salinity and low-moisture-salinity habitats of the Ebinur LakeWetland National Nature Reserve.ResultsThe results showed that (1) The soil moisture and electrical conductivity heterogeneity showed significant differences between the two moisture-salinity habitats. …”
Get full text

Seasonal Variations in Chemical Composition and Antibacterial and Antioxidant Activities of <i>Rosmarinus officinalis</i> L. Essential Oil from Southwestern Romania by Ludovic Everard Bejenaru, Adina-Elena Segneanu, Cornelia Bejenaru, Andrei Biţă, Felicia Tuţulescu, Antonia Radu, Maria Viorica Ciocîlteu, George Dan Mogoşanu

“…To analyze the constituents of RoEO, a comprehensive gas chromatography/mass spectrometry (GC/MS) method was employed. The analysis aimed to identify and quantify the various components by comparing their mass spectra with reference spectra from the National Institute of Standards and Technology (NIST) Library 2020. …”
Get full text

Analysis Model of Giant Prawns Population (Macrobrachium rosenbergii) in Estuary Edge of Sembakung Waters, Nunukan, Indonesia by Agus Indarjo, Gazali Salim, Christine Dyta Nugraeni, Lukman Yudho Prakoso, Permana Ari Soejarwo, Rukisah Rukisah, Yen Thi Hong Pham, Achmad Daengs GS, Hariyadi Hariyadi, Abdul Jabarsyah

“…The data collection included data of sex, total length, and the total weight of giant prawns. While analysis was employed for growth pattern, condition index, Von Bertalanffy growth, and mor- tality. …”
Get full text

CD276 regulates the immune escape of esophageal squamous cell carcinoma through CXCL1–CXCR2 induced NETs by Yu Liang, Shuang Chen, Zhi Chen, Fang Peng, Qianwen Liu, Gan Xiong, Caihua Zhang, Maosheng Cheng, Rongsong Ling, Demeng Chen, Qimin Zhou

“…Moreover, IHC, flow cytometry, and immunofluorescence techniques were employed to measure immune cell proportions in ESCC. …”
Get full text

Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile aci... by Jian Chen, Kanwar K. Malhi, Xiaowei Li, Xiangwen Xu, Jianxun Kang, Bichen Zhao, Yaru Xu, Xuenan Li, Jinlong Li

“…Western blotting and immunofluorescence were employed to assess protein expression levels. Our results indicated that metasilicate-based AMW effectively alleviated weaning-induced colonic or liver morphological injury and inflammatory response, as well as liver cholesterol metabolism disorders. …”
Get full text