Relationship between CTF1 gene expression and prognosis and tumor immune microenvironment in glioma by Hongqing Cai, Shen Tian, Angsi Liu, Guanchao Xie, Hongsheng Zhang, Xiaogang Wu, Jinghai Wan, Sai Li

“…To assess the impact on glioma cell proliferation and apoptosis, we employed various assays, including the Cell Counting Kit-8, colony formation assay, and flow cytometry. …”
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Repurposing the prostaglandin analogue treprostinil and the calcium-sensing receptor modulator cinacalcet to revive cord blood as an alternate source of hematopoietic stem and prog... by Michaela Prchal-Murphy, Julia Zehenter, Marlene Fischer, Anita Pirabe, Madeleine Themanns, Behnaz Afrashteh, Eva Maria Putz, Karoline Kollmann, José Basílio, José Basílio, Manuel Salzmann, Wolfgang Strohmaier, Günther Krumpl, Alex Farr, Veronika Sexl, Michael Freissmuth, Eva Zebedin-Brandl

“…In our study, we investigated the combined effects of treprostinil, a prostaglandin analog, and cinacalcet, a calcium-sensing receptor modulator, on the reconstitution of hematopoiesis.MethodsA Lineage Cell Depletion Kit was employed to isolate lineage-negative (lin−) HSPCs from mouse bone marrow. …”
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Position in proton Bragg curve influences DNA damage complexity and survival in head and neck cancer cells by Tim Heemskerk, Celebrity Groenendijk, Marta Rovituso, Ernst van der Wal, Wouter van Burik, Konstantinos Chatzipapas, Danny Lathouwers, Roland Kanaar, Jeremy M.C. Brown, Jeroen Essers

“…A custom water phantom setup is employed to accurately position the samples within the Bragg curve. …”
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Predictive Factors for Failure of Noninvasive Ventilation in Adult Intensive Care Unit: A Retrospective Clinical Study by Qimin Chen, Ming Liu, Bo Liu, Wei Li, Daixiu Gao, Lulu Xie, Yu Wu, Liang Li, Ying Liu, Ying Wang, Tang Yan, Yuanyi Liu, Yumei Cheng, Xu Liu, Feng Shen

“…Logistic regression was employed to determine the risk factor(s) for NIV, and a predictive model for NIV outcome was set up using risk factors. …”
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Discovery of anti-Ebola virus multi-target inhibitors from traditional Chinese medicine database using molecular screening, biophysical investigation, and binding free energy calcu... by Abbas Khan, Abrar Mohammad Sayaf, Anwar Mohammad, Fahad M. Alshabrmi, Tarek Benameur, Dong-Qing Wei, Kar Kheng Yeoh, Abdelali Agouni

“…Materials and methods: In this study, we employed in silico methods to assess the inhibitory activity of natural products from traditional Chinese medicine (TCM) against four EBOV proteins that are crucial for viral replication and assembly: VP40, VP35, VP30, and VP24. …”
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Baseline atherogenic index of plasma and its trajectory predict onset of type 2 diabetes in a health screened adult population: a large longitudinal study by Yongbing Sun, Xinbei Lin, Zhi Zou, Caiwen Zhao, Ao Liu, Jing Zhou, Zhonglin Li, Xiaoling Wu, Shewei Dou, Jiadong Zhu, Tao Li, Xue Lv, Yong Wang, Yongli Li

“…The Kaplan-Meier method was employed to analyze the incidence of T2DM across different AIP groups. …”
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Differentiating Pulmonary Nodule Malignancy Using Exhaled Volatile Organic Compounds: A Prospective Observational Study by Guangyu Lu, Zhixia Su, Xiaoping Yu, Yuhang He, Taining Sha, Kai Yan, Hong Guo, Yujian Tao, Liting Liao, Yanyan Zhang, Guotao Lu, Weijuan Gong

“…Analyzing exhaled volatile organic compounds (VOCs) shows promise, yet its effectiveness in assessing the malignancy of pulmonary nodules remains underexplored. Methods Employing a prospective study design from June 2023 to January 2024 at the Affiliated Hospital of Yangzhou University, we assessed the malignancy of pulmonary nodules using the Mayo Clinic model and collected exhaled breath samples alongside lifestyle and health examination data. …”
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A preliminary study of linear accelerator-based spatially fractionated radiotherapy by Young Kyu Lee, Yunji Seol, Byeong Jin Kim, Kyu Hye Choi, Ji Hyun Hong, Chan-beom Park, Sun Hwa Kim, Hyeong Wook Park, Wonjoong Cheon, Young Nam Kang, Byung‑Ock Choi

“…The plans were optimized employing a 3 × 3 × 3 lattice structure with vertex diameters ranging from 0.5 to 2.0 cm and separations from 1.0 to 5.0 cm. …”
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Disrupting USP39 deubiquitinase function impairs the survival and migration of multiple myeloma cells through ZEB1 degradation by Jessy Sirera, Saharnaz Sarlak, Manon Teisseire, Alexandrine Carminati, Victoria J. Nicolini, Coline Savy, Patrick Brest, Thierry Juel, Christophe Bontoux, Marcel Deckert, Mickael Ohanna, Sandy Giuliano, Maeva Dufies, Gilles Pages, Frederic Luciano

“…Gene Set Enrichment Analysis (GSEA) was employed to correlate USP39 mRNA levels with clinical outcomes in MM patients. …”
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The AMD-associated genetic polymorphism CFH Y402H confers vulnerability to Hydroquinone-induced stress in iPSC-RPE cells by Angela Armento, Inga Sonntag, Ana-Cristina Almansa-Garcia, Merve Sen, Sylvia Bolz, Blanca Arango-Gonzalez, Ellen Kilger, Ruchi Sharma, Kapil Bharti, Rosario Fernandez-Godino, Berta de la Cerda, Simon J. Clark, Simon J. Clark, Simon J. Clark, Marius Ueffing

“…In our study, we examined the cell characteristics of the retinal pigment epithelium (RPE) cells, which play a major role in retinal homeostasis and stability and which are synonymously linked to AMD.MethodsHere, we employ RPE cells derived from induced pluripotent stem cells (iPSC) generated from donors, carrying either homozygous 402Y (low risk) or 402H (high risk) variants of the CFH gene. …”
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A new signature associated with anoikis predicts the outcome and immune infiltration in nasopharyngeal carcinoma by Yonglin Luo, Wenyang Wei, Yaxuan Huang, Jun Li, Weiling Qin, Quanxiang Hao, Jiemei Ye, Zhe Zhang, Yushan Liang, Xue Xiao, Yonglin Cai

“…Methods By using data from the Gene Expression Omnibus (GEO) database, we employed machine learning methods to discover prognostic ARGs and create a risk score. …”
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Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists by Fabiana Evaristo-Mendonça, Gabriela Sardella-Silva, Tais Hanae Kasai-Brunswick, Raquel Maria Pereira Campos, Pablo Domizi, Marcelo Felippe Santiago, Ricardo Augusto de Melo Reis, Rosalia Mendez-Otero, Victor Túlio Ribeiro-Resende, Pedro Moreno Pimentel-Coelho

“…Although rat BM-MSCs (rBM-MSCs) have been widely tested in preclinical research, most preconditioning studies have employed human and mouse BM-MSCs. Herein, we investigated whether rBM-MSCs modify their phenotype and paracrine functions in response to Toll-like receptor (TLR) agonists. …”
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Speciation Analysis of Arsenic Compounds by High-Performance Liquid Chromatography in Combination with Inductively Coupled Plasma Dynamic Reaction Cell Quadrupole Mass Spectrometry... by Hai Anh Vu, Manh Ha Nguyen, Hong-An Vu-Thi, Quan Do-Hong, Xuan Hoang Dang, Thi Ngoc Bich Nguyen, Hong Quan Trinh, Thuy Ly Bich, Tien-Thanh Nguyen, Dung Le-Van, Minh Binh Tu, Dinh Binh Chu

“…Five arsenic compounds were separated on a Hamilton PRP X100 strong anion-exchange column employed with the mobile phase that is compatible with mass spectrometry, containing ammonium carbonate, methanol, and disodium ethylenediaminetetraacetic acid. …”
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Exploring Disparities in Gill Physiological Responses to NaHCO₃-Induced Habitat Stress in Triploid and Diploid Crucian Carp (<i>Carassius auratus</i>): A Comprehensive I... by Shicheng Han, Lin Han, Fangying Yuan, Wenzhi Liu, Jing Wang, Xiaofeng Jin, Yanchun Sun

“…<b>Methods</b>: This investigation explores the effects of 20 and 60 mmol NaHCO₃ stress over 30 days on the gills of diploid and triploid crucian carp, employing histological, biochemical, and multi-omic analyses. …”
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Development and validation of a novel artificial intelligence algorithm for precise prediction the postoperative prognosis of esophageal squamous cell carcinoma by Zichen Wang, Zhihan Xiao, Tongyu Zhang, Meiyou Lu, Hai Li, Jing Cao, Jianan Zheng, Yichan Zhou, Juncheng Dai, Cheng Wang, Liang Chen, Jing Xu

“…Methods We conducted LASSO regression analysis of protein-coding CTGs and clinical characteristics from 119 pathologically confirmed ESCC patients to recognize powerful predictive variables. We employed nine supervised machine learning classifiers and integrated best predictive machine learning classifiers by weighted voting method to construct an ensemble model called PPMESCC. …”
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Improving daily reference evapotranspiration forecasts: Designing AI-enabled recurrent neural networks based long short-term memory by Mumtaz Ali, Jesu Vedha Nayahi, Erfan Abdi, Mohammad Ali Ghorbani, Farzan Mohajeri, Aitazaz Ahsan Farooque, Salman Alamery

“…The main purpose of this investigation was to forecast the daily ETo trends at Melbourne and Sydney stations in Australia, where several cutting-edge machine learning methodologies were employed. The modeling approach encompassed the implementation of Neural Network (NN), Deep Learning (DL), Recurrent Neural Networks (RNN), RNN based Long Short-Term Memory (RNN-LSTM), and Convolutional Neural Network based LSTM (CNN-LSTM) to forecast daily ETo using historical meteorology data. …”
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Admission outcomes and their associated factors among children admitted to the paediatric emergency unit within 24 hours of Dilla University Referral Hospital, Ethiopia, 2023: a cr... by Binyam Tariku Seboka, Tomas Yeheyis, Yihenew Ayehu Dessie, Samuel Jigso Dube, Abel Desalegn Demeke, Mekdes Mekonnen Feleke, Abdo Hajo Jarso, Asresu Feleke Bati, Etaferaw Udo, Selam Tadele Markos, Chalachew Kassaw, Amdehiwot Aynalem Debebe, Ephrem Geja Gechere

“…Background Children in paediatric emergency units are those who need special attention, and unless treated early, they are a vulnerable population to unwanted outcomes like death, discharge against medical advice or referral to other institutions within 24 hours.Objectives To assess admission outcomes and their associated factors among children admitted to the paediatric emergency unit of Dilla University Referral Hospital, Ethiopia, 2023.Methods An institution-based cross-sectional study design was employed among children admitted to the paediatric emergency unit at Dilla University Referral Hospital from 8 May 2023 to 8 June 2023. …”
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A novel way to use cross-validation to measure connectivity by machine learning allows epilepsy surgery outcome prediction by Karla Ivankovic, Alessandro Principe, Justo Montoya-Gálvez, Linus Manubens-Gil, Riccardo Zucca, Pablo Villoslada, Mara Dierssen, Rodrigo Rocamora

“…The classifier generalization performance estimated by cross-validation was employed as a continuous measure of connectivity change. …”
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The relationship between impostor phenomenon and emotional exhaustion among Chinese nurses: the mediating role of bi-directional work-family conflict by Yuan Li, Yuan Li, Yuan Li, Fangxinrui Qiu, Biru Luo, Biru Luo, Yanling Hu, Yanling Hu, Yanling Hu, Jie Li, Ying Xin Li, Ying Xin Li, Ying Xin Li, Ping He, Jinbo Fang

“…SPSS with Hayes’s PROCESS v4.2 Macro was employed to examine the mediation model using bootstrap techniques.ResultsAfter controlling for confounding factors, impostor phenomenon was found to have a direct positive effect on emotional exhaustion (𝛽 = 0.134, 95% CI [0.122 to 0.145]); the two dimensions of work–family conflict, work interfering with family (𝛽 = 0.099, 95% CI [0.090 to 0.109]) and family interfering with work (𝛽 = 0.017, 95% CI [0.012 to 0.022]), served as parallel mediators in the relationship between impostor phenomenon and emotional exhaustion. …”
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S100A13-driven interaction between pancreatic adenocarcinoma cells and cancer-associated fibroblasts promotes tumor progression through calcium signaling by Liuyuan Xia, Xin Guo, Dong Lu, Yixin Jiang, Xiaohui Liang, Yiwen Shen, Jiayi Lin, Lijun Zhang, Hongzhuan Chen, Jinmei Jin, Xin Luan, Weidong Zhang

“…Methods Bioinformatic analysis was employed to predict the pathogenic potential of CAFs and S100A13. …”
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