Recognition of HER2 expression in hepatocellular carcinoma and its significance in postoperative tumor recurrence by Ji‐Hua Shi, Wen‐Zhi Guo, Yang Jin, Hua-Peng Zhang, Chun Pang, Jie Li, Pål‐Dag Line, Shui‐Jun Zhang

“…Western Blotting and immunohistochemistry were employed to test expression of HER2, E‐cadherin, and Vimentin. …”
Get full text

A combined wavelet analysis-quantile mapping (WA-QM) method for bias correction: capturing the intra-annual temporal patterns in climate model precipitation simulations and project... by Xia Wu, Zhu Liu, Qingyun Duan

“…Furthermore, WA could be an effective tool to overcome QM’s limitation in capturing intra-annual temporal patterns. The WA approach employed discrete wavelet transformation to decompose GCM data into various frequency bands and then adjusted their standard deviations and signs to ensure that their relative relationships were consistent with those in observations. …”
Get full text

TransferBAN-Syn: a transfer learning-based algorithm for predicting synergistic drug combinations against echinococcosis by Haitao Li, Haitao Li, Yuanyuan Chu, Yuanyuan Chu, Liyuan Jiang, Liyuan Jiang, Lei Li, Lei Li, GuoDong Lv, Yuansheng Liu, Chunhou Zheng, Chunhou Zheng, Yansen Su, Yansen Su

“…Specifically, TransferBAN-Syn contains a drug interaction feature representation module, a disease feature representation module, and a prediction module, where the bilinear attention network is employed in the drug interaction feature representation module to deeply extract the fusion feature of drug combinations. …”
Get full text

Study on optimization of extraction and purification processes for total flavonoids from Lycopi herba roots and their anti-proliferative effects on fibrous synoviocytes in human rh... by Jie Chen, Chunyang Jiang, Zhenyu Liu, Panpan Wang, Qiang Ma, Nong Zhou

“…Subsequently, macroporous resin chromatography was employed for flavonoid purification, with AB-8 resin exhibiting the highest performance, achieving adsorption and desorption rates of 79.64 ± 1.51 % and 88.61 ± 1.02 %, respectively. …”
Get full text

A semantic strength and neural correlates in developmental dyslexia by Sladjana Lukic, Sladjana Lukic, Sladjana Lukic, Fei Jiang, Maria Luisa Mandelli, Maria Luisa Mandelli, Ting Qi, Sarah M. Inkelis, Sarah M. Inkelis, Emily Rosenthal, Emily Rosenthal, Zachary Miller, Zachary Miller, Emma Wellman, Emma Wellman, Silvia A. Bunge, Silvia A. Bunge, Silvia A. Bunge, Maria Luisa Gorno-Tempini, Maria Luisa Gorno-Tempini, Christa Watson Pereira, Christa Watson Pereira

“…First, we adjusted performance on semantic fluency by letter fluency and created a residual score that was used to separate participants into high (residual >0) or average (residual <0) semantic performance groups. We then employed a psycholinguistic clustering and switching approach to the semantic fluency task and performed dynamic task-free rs-fMRI connectivity analysis to reveal group differences in brain dynamics.ResultsHigh and average semantic fluency groups were well-matched on demographics and letter fluency but differed on their psycholinguistic patterns on the semantic fluency task. …”
Get full text

Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in Greece by Anestis Tsitsos, Alexandros Damianos, Konstantinos Kiskinis, Vasilios Tsiouris, Ilias Tirodimos, Nikolaos Soultos, Anna Papa, Vangelis Economou

“…Strains were selectively isolated and identified via MALDI-TOF MS, which was also employed to identify possible relatedness. <i>E. coli</i> isolates were further classified into phylogenetic groups. …”
Get full text

Influence of Irradiance, Flow Rate, Reactor Geometry, and Photopromoter Concentration in Mineralization Kinetics of Methane in Air and in Aqueous Solutions by Photocatalytic Membra... by Ignazio Renato Bellobono, Mauro Rossi, Andrea Testino, Franca Morazzoni, Riccardo Bianchi, Giulia de Martini, Paola Maria Tozzi, Rodica Stanescu, Cristina Costache, Liliana Bobirica, Mauro Luigi Bonardi, Flavia Groppi

“…A kinetic model was employed, from which, by a set of differential equations, four final optimised parameters, k1 and K1, k2 and K2, were calculated, which is able to fit the whole kinetic profile adequately. …”
Get full text

Analysis of Soil Microbial Community Structure and Function in <i>Morchella esculenta</i> Habitats in Jilin Province by Qi Yan, Peng Wang, Zhushan Liu, Ya Yu, Xiao Tan, Xiao Huang, Jiawei Wen, Weidong Zhang

“…Metagenome sequencing technology was employed to analyze the structure and function of the rhizosphere microbial communities. …”
Get full text

Eco-friendly synthesis of silver nanoparticles: Enhancing optimization reaction, characterization, and antimicrobial properties with Lantana camara from geothermal area by Pati Kemala, Rinaldi Idroes, Khairan Khairan, Muliadi Ramli, Binawati Ginting, Zuchra Helwani, Rifki Aulia, Ghazi Mauer Idroes, Muhammad Yusuf, Rustam Efendi

“…Reaction optimization was performed using response surface methodology (RSM), employing a central composite design (CCD) approach. …”
Get full text

Interrelationships between social exclusion, mental health and wellbeing in adolescents: insights from a national Youth Survey by K. Filia, S. M. Teo, N. Brennan, T. Freeburn, D. Baker, V. Browne, A. Watson, J. Menssink, A. Prasad, E. Killackey, P. D. McGorry, S. M. Cotton, C. X. Gao

“…Abstract Aims Adolescence is a critical developmental phase during which young people are vulnerable to the experiences of mental ill-health and social exclusion (consisting of various domains including education and employment, housing, finances and social supports and relationships). …”
Get full text

Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal... by Nanquan Rao, Xiaotong Wang, Jing Xie, Jingzhi Li, Yue Zhai, Xiaoxia Li, Tengjiaozi Fang, Yuanyuan Wang, Yuming Zhao, Lihong Ge

“…At 8 weeks after administration, real-time PCR, immunohistochemistry (IHC), and electron microscopy were employed to examine pathological changes in glomerular and tubulointerstitial tissue. …”
Get full text

Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. …”
Get full text

Assessment approach for conservation effectiveness and gaps for endangered species based on habitat suitability: A case study of alpine musk deer in western China by Feng Jiang, Pengfei Song, Jingjie Zhang, Dongwu Wang, Renhong Li, Chengbo Liang, Tongzuo Zhang

“…This study integrated data from 274 alpine musk deer occurrence points, obtained through infrared camera monitoring, field surveys, and literature reviews, with environmental variables, employing the MaxEnt model to analyze the current spatial distribution of suitable habitats and priority conservation areas in Western China. …”
Get full text

Causal associations between osteoporosis and HBV infection across Asian and European populations: evidence from Mendelian randomization and colocalization analysis by Zhengnan Li, Jiarui Cao, Ke Li, Yixin Wu, Zhanpeng Luo, Rui Cao, Zhiheng Cheng, Zhendong Tian, Yiyang Han, Yuping Lai, Bangqi Wang, Shen Chen

“…Various MR methods, including inverse variance weighted (IVW), MR Egger, weighted median, simple median and simple mode were employed to estimate the association between HBV infection and osteoporosis. …”
Get full text

Enhanced fetal hemoglobin production via dual-beneficial mutation editing of the HBG promoter in hematopoietic stem and progenitor cells for β-hemoglobinopathies by Prathibha Babu Chandraprabha, Manoj Kumar K. Azhagiri, Vigneshwaran Venkatesan, Wendy Magis, Kirti Prasad, Sevanthy Suresh, Aswin Anand Pai, Srujan Marepally, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, David I. K. Martin, Saravanabhavan Thangavel

“…However, achieving this goal requires precise nucleotide conversions in hematopoietic stem and progenitor cells (HSPCs) at therapeutic efficiency, which remains a challenge. Methods We employed Cas9 RNP-ssODN-mediated homology-directed repair (HDR) gene editing to mimic two naturally occurring HBG promoter point mutations; -175T > C, associated with high HbF levels, and −158 C > T, a common polymorphism in the Indian population that induces HbF under erythropoietic stress, in HSPCs. …”
Get full text

Manganese-based immunotherapy synergized with novel supramolecular hydrogel: Advancing localized immune activation strategies in squamous cell carcinoma by Tianqing Wang, Xiaopei Hu, Xin Tian, Ying Wang, Shiyu Zhang, Silu Sun, Ning Ji, Xiaobo Luo, Qianming Chen, Xin Zeng, Hang Zhao, Jing Li

“…In this study, we introduce a novel approach employing manganese-based immunotherapy (Mn-IT) for the effective treatment of HNSCC. …”
Get full text

Predicting breast cancer prognosis using PR and PIK3CA biomarkers: a comparative analysis of diagnostic groups by Yuting Feng, Qingzhen Song, Lei Yan, Ruoqi Li, Mengqin Yang, Peng Bu, Jing Lian

“…To evaluate the variability of clinicopathologic features among these groups, t tests and ANOVA were employed. The influence of these variables on survival was analyzed via Cox regression. …”
Get full text

A systematic review and mapping exercise to assess the content validity of patient-reported outcome measures for adults having reconstructive surgery of the lower limb by Jennie Lister, Sophie James, Hemant K. Sharma, Catherine Hewitt, Helen Fulbright, Heather Leggett, Catriona McDaid

“…Many patient-reported outcome measures (PROMs) are employed to assess these impacts; however, there are concerns that they do not adequately capture all outcomes important to patients, and may lack content validity in this context. …”
Get full text

Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network by Qi Xiao, Jingjing Shi, Lixian Wang, Guoping Zhao, Yanfei Zhang

“…While enhanced production of isopentanol has been achieved in a variety of chassis, the fermentation yield has not yet reached levels suitable for industrial-scale production. In this study, we employed a continuous perturbation tool to construct a genome-scale perturbation library, combined with an isopentanol biosensor to screen for high-yielding mutants. …”
Get full text

Gene networks for use in metabolomic data analysis of blood plasma from patients with postoperative delirium by V. A. Ivanisenko, N. V. Basov, A. A. Makarova, A. S. Venzel, A. D. Rogachev, P. S. Demenkov, T.  V. Ivanisenko, M. A. Kleshchev, E. V. Gaisler, G. B. Moroz, V. V. Plesko, Y. S. Sotnikova, Y. V. Patrushev, V. V. Lomivorotov, N. A. Kolchanov, A. G. Pokrovsky

“…The analysis revealed four significantly different sphingomyelin contents in patients with POD compared to those who did not develop POD (control group). Employing gene network reconstruction, we perceived a set of 82 regulatory enzymes affiliated with the genetic coordination of the sphingolipid metabolism pathway. …”
Get full text