Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification by Jin-Ha Song, Seung-Eun Son, Ho-Won Kim, Seung-Ji Kim, Se-Hee An, Chung-Young Lee, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Kang-Seuk Choi, Kang-Seuk Choi

“…In this study, we introduce a centrifuge-free, rapid detection method for on-site detection of H5Nx viruses that combines magnetic bead-based ribonucleoprotein (RNP) purification and concentration with a CRISPR-Cas12a system that is independent of the protospacer adjacent motif (PAM) sequence. Our approach employs anti-NP monoclonal antibodies for the targeted isolation of RNA bound to RNPs, facilitating a quick and specific RNA extraction process that negates the need for centrifugation. …”
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Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model by Tiantian Hu, Rui Chen, Yu Qian, Ke Ye, Xingyao Long, Kun-Young Park, Xin Zhao

“…Thus, LF-HFY02 may be potentially employed as a probiotic strain. In conclusion, soybean milk fermented by LF-HFY02 can increase the content of antioxidant factors and the activity of antioxidant enzymes by regulating gene and protein expression, and finally inhibit the process of tissue cell peroxidation, and improve the oxidative damage of mouse skin and liver. …”
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Genomic insights into the specialisation and selection of the Jinding duck by H. Chen, Y. Huang, J. Xue, K. luo, H. Tang, S. Zheng, Y. Xiong, Y. Wu, J. Li, R. Xuan, R. Xiong, Y. Gong, X. Fang, L. Wang, J. Miao, J. Zhou, H. Tan, Y. Wang, L. Wu, J. Ouyang, Y. Shen, X. Yan

“…Here, we focus on the Jinding Duck (JDD), a breed renowned for its prolific egg-laying traits. Employing whole-genome resequencing data from 325 individuals across five Chinese indigenous duck breeds, we aimed to dissect the unique population structure and assess the genetic diversity within the JDD cohort. …”
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Serological insights from SARS-CoV-2 heterologous prime and boost responses in Thailand by Daniel Ward, Lapasrada Pattarapreeyakul, Rujiraporn Pitaksalee, Naphatcha Thawong, Waritta Sawaengdee, Suthida Tuntigumthon, Catriona Patterson, Kevin Tetteh, Susana Campino, Panadda Dhepakson, Surakameth Mahasirimongkol, Taane G. Clark

“…Abstract During the COVID-19 pandemic, heterologous vaccination strategies were employed to alleviate the strain on vaccine supplies. …”
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Selection of suitable reference lncRNAs for gene expression analysis in Osmanthus fragrans under abiotic stresses, hormone treatments, and metal ion treatments by Yingting Zhang, Yingting Zhang, Qingyu Yan, Hui Xia, Xiangling Zeng, Xiangling Zeng, Xiangling Zeng, Jie Yang, Jie Yang, Jie Yang, Xuan Cai, Xuan Cai, Xuan Cai, Zeqing Li, Zeqing Li, Hongguo Chen, Hongguo Chen, Hongguo Chen, Jingjing Zou, Jingjing Zou, Jingjing Zou

“…Despite its importance, research on long non-coding RNAs (lncRNAs) in O. fragrans has been constrained by the absence of reliable reference genes (RGs).MethodsWe employed five distinct algorithms, i.e., delta-Ct, NormFinder, geNorm, BestKeeper, and RefFinder, to evaluate the expression stability of 17 candidate RGs across various experimental conditions.Results and discussionThe results indicated the most stable RG combinations under different conditions as follows: cold stress: lnc00249739 and lnc00042194; drought stress: lnc00042194 and lnc00174850; salt stress: lnc00239991 and lnc00042194; abiotic stress: lnc00239991, lnc00042194, lnc00067193, and lnc00265419; ABA treatment: lnc00239991 and 18S; MeJA treatment: lnc00265419 and lnc00249739; ethephon treatment: lnc00229717 and lnc00044331; hormone treatments: lnc00265419 and lnc00239991; Al3+ treatment: lnc00087780 and lnc00265419; Cu2+ treatment: lnc00067193 and 18S; Fe2+ treatment: lnc00229717 and ACT7; metal ion treatment: lnc00239991 and lnc00067193; flowering stage: lnc00229717 and RAN1; different tissues: lnc00239991, lnc00042194, lnc00067193, TUA5, UBQ4, and RAN1; and across all samples: lnc00239991, lnc00042194, lnc00265419 and UBQ4. …”
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The ArcB kinase sensor participates in the phagocyte-mediated stress response in Salmonella Typhimurium by Coral Pardo-Esté, Coral Pardo-Esté, Francisca Urbina, Nicolas Aviles, Nicolas Pacheco, Alan Briones, Carolina Cabezas, Vicente Rojas, Valentina Pavez, Yoelvis Sulbaran-Bracho, Yoelvis Sulbaran-Bracho, Alejandro A. Hidalgo, Juan Castro-Severyn, Juan Castro-Severyn, Claudia P. Saavedra

“…This study contributes to the understanding of the pathogenicity and adaptation mechanisms that Salmonella employs to establish a successful infection in its host.…”
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Effects of acteoside from Cistanche tubulosa on the plasma metabolome of cancer-related fatigue mice inoculated with colon cancer cells by Shilei Zhang, Fukai Gong, Jiali Liu, Shuping You, Tao Liu, Jianhua Yang, Junping Hu

“…Finally, liquid chromatography-mass spectrometry (LC/MS) was employed to investigate alterations in the plasma metabolic profile of tumor-bearing mice with CRF in response to ACT treatment, and the affinity between metabolite-associated proteins and ACT was verified by Surface plasmon resonance (SPR) assay.ResultsOur study demonstrated the presence of CRF in the colon cancer mouse model, with the severity of fatigue increasing alongside tumor growth. …”
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A Method for Quantifying Mung Bean Field Planting Layouts Using UAV Images and an Improved YOLOv8-obb Model by Kun Yang, Xiaohua Sun, Ruofan Li, Zhenxue He, Xinxin Wang, Chao Wang, Bin Wang, Fushun Wang, Hongquan Liu

“…In this work, an unmanned aerial vehicle (UAV) was employed to capture visible light images of mung bean seedlings in a field across three height gradients of 2 m, 5 m, and 7 m following a time series approach. …”
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Deciphering the role of miRNA-mRNA interactions in cerebral vasospasm post intracranial hemorrhage by Xiang Chu, Xiang Chu, Xiyan Zhu, Honghao Xu, Wenbing Zhao, Debin Guo, Xing Chen, Jinze Wu, Lei Li, Hao Wang, Jun Fei

“…Further functional enrichment and KEGG analysis revealed that these DEGs were enriched in several terms and pathways, including the PI3K/AKT/mTOR signaling pathway, oxidative phosphorylation pathway, RNA degradation, and folate biosynthesis signaling pathway. By employing the degree scores method for each gene, we identified the top 10 genes and developed a protein-protein interaction (PPI) network. …”
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Effect of pretreatment type on the physico-chemical properties of activated carbons derived from an invasive weed Prosopis juliflora: potential applications by Subramaniyasharma Sivaraman, Saravanan Ramiah Shanmugam, Ponnusami Venkatachalam, Ratheeshkumar Shanmugam, Arul Chan Basha, Noori M Cata Saady

“…Principal component analysis was employed to elucidate the relationships between different activation methods and the physico-chemical properties of the activated carbons. …”
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Determination of Pharmaceutical Residues by UPLC-MS/MS Method: Validation and Application on Surface Water and Hospital Wastewater by Bui Van Hoi, Cam-Tu Vu, Lan-Anh Phung-Thi, Thao Thi Nguyen, Phuong Thanh Nguyen, Huong Mai, Phuong-Thu Le, Thanh-Hien Nguyen, Dao Thanh Duong, Hue Nguyen Thi, Dung Le-Van, Dinh Binh Chu

“…The method includes enrichment and clean-up steps by solid phase extraction using mix-mode cation exchange, followed by identification and quantification using an ultrahigh-performance liquid chromatography and tandem mass spectrometry and employing electrospray ionization (UPLC-ESI-MS/MS). …”
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Density functional theory calculation for understanding the roles of biochar in immobilizing exchangeable Al3 + and enhancing soil quality in acidic soils by Debo He, Xinyi Liu, Dongni Hu, Ping Lei, Jinbo Zhang, Zhixin Dong, Bo Zhu

“…Through incubation experiments, we compared the effects of biochar and lime on soil properties. Additionally, we employed density functional theory (DFT) calculations to elucidate the mechanisms underlying biochar's ability to immobilize exchangeable Al3+. …”
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Exploring the role of ELOVLs family in lung adenocarcinoma based on bioinformatic analysis and experimental validation by Zihan Wang, Wenjing Cui, Long Liang, Jingge Qu, Yuqiang Pei, Danyang Li, Ying Luo, Yue Zhang, Yifan Qiu, Yongchang Sun

“…Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, cell counting kit-8 (CCK8), colony formation, wound healing, transwell migration assays and fatty acid metabolism detection were employed to confirm the significant role of ELOVL6 in vitro experiment. …”
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Bidirectional relationship between depression and activities of daily living and longitudinal mediation of cognitive function in patients with Parkinson’s disease by Yue Xu, Yue Xu, Durong Chen, Meiqi Dong, Yun Zhang, Yun Zhang, Hongmei Yu, Hongmei Yu, Hongmei Yu, Yanqing Han, Yanqing Han, Yanqing Han

“…The cross-lagged panel model (CLPM) was employed to analyze the reciprocal relationship between depression and ADL. …”
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Ganciclovir Resistance-Linked Mutations in the HCMV <i>UL97</i> Gene: Sanger Sequencing Analysis in Samples from Transplant Recipients at a Tertiary Hospital in Southern Brazil by Anna Caroline Avila da Rocha, Grazielle Motta Rodrigues, Alessandra Helena da Silva Hellwig, Dariane Castro Pereira, Fabiana Caroline Zempulski Volpato, Afonso Luís Barth, Fernanda de-Paris

“…<b>Methods:</b> A nested-PCR approach combined with a touchdown PCR method was employed to enhance the sensitivity and specificity of the sequencing analysis. …”
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Exploring the impact of genetic factors and fatty acid quality on visceral and overall Fat levels in overweight and obese women: a genetic risk score study by Niloufar Rasaei, Atefeh Tavakoli, Saba Mohammadpour, Mehdi Karimi, Alireza Khadem, Azam Mohamadi, Seyedeh Fatemeh Fatemi, Fatemeh Gholami, Khadijeh Mirzaei

“…We have used a comprehensive food frequency questionnaire (FFQ) to evaluate dietary intake and the fatty acids quality indexes. We have employed standard methods to measure biochemical factors, anthropometrics, and physical activity levels. …”
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DNMT1-driven methylation of RORA facilitates esophageal squamous cell carcinoma progression under hypoxia through SLC2A3 by Wenjian Yao, Linlin Shang, Yinghao Wang, Lei Xu, Yu Bai, Mingyu Feng, Xiangbo Jia, Sen Wu

“…DNA pull-down assay and proteomic analysis were employed to identify proteins bound to the RORA promoter. …”
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A Simple Machine Learning-Based Quantitative Structure–Activity Relationship Model for Predicting pIC₅₀ Inhibition Values of FLT3 Tyrosine Kinase by Jackson J. Alcázar, Ignacio Sánchez, Cristian Merino, Bruno Monasterio, Gaspar Sajuria, Diego Miranda, Felipe Díaz, Paola R. Campodónico

“…<b>Methods:</b> Using a dataset which was 14 times larger than those employed in prior studies (1350 compounds with 1269 molecular descriptors), we trained a random forest regressor, chosen due to its superior predictive performance and resistance to overfitting. …”
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Cytoplasmic genetic diversity of potato varieties bred in Russia and FSU countries by Т. A. Gavrilenko, N. S. Klimenko, N. V. Alpatieva, L. I. Kostina, V. A. Lebedeva, Z. Z. Evdokimova, O. V. Apalikova, L. Y. Novikova, O. Yu. Antonova

“…In this work, a set of 6 cytoplasmic markers was employed to describe cytoplasmic genetic diversity of 185 potato cultivars bred in Russia and FSU countries. …”
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Monitoring and evaluation in disaster management courses: a scoping review by George Teo Voicescu, Hamdi Lamine, Andra Elena Loșonți, Eugenia Maria Lupan-Mureșan, Sonia Luka, José García Ulerio, Luca Ragazzoni, Francesco Della Corte, Marta Caviglia

“…Results Fifty-seven articles met the inclusion and exclusion criteria, described DM training targeting healthcare and non-healthcare professionals and employed diverse teaching methods and topics. Five studies reported using monitoring, while all reported an evaluation methodology. …”
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