Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro by Jianeng Li, Changle Zhao, Lei Liu, Shuqing Zheng, Deshou Wang, Changwei Shao, Jing Wei

“…Furthermore, the sustained proliferation of SC1 was found to depend on a combination of various factors, particularly the extract from Nile tilapia embryos. Collectively, our findings indicate the successful establishment of a Southern catfish spermatogonial stem cell line that exhibits sustained proliferation and sperm production in vitro. …”
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Microtubule inhibition as a proposed mechanism for the anthelmintic effect of phytochemicals isolated from Cicerbita alpina by Mark James Horgan, Ines Sigg, Ioanna Poulopoulou, Francisco J. Rodriguez-Mejias, Eva Albertini, Pietro Fusani, Florian Fischer, Eftychia Martinidou, Daniela Schuster, Stefan Martens, Pidder Jansen Dürr, Matthias Gauly, Hermann Stuppner, Alexander Weiss, Veronika Temml, Bianka Siewert

“…Liquid–liquid fractions were prepared from a methanolic extract of the aerial parts and were submitted in parallel to embryo development (ED), worm motility (WMT), and cytotoxicity assays for anthelmintic and toxicity evaluations. …”
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Generation of live mice from haploid ESCs with germline-DMR deletions or switch by Yongjian Ma, Meng Yan, Zhenfei Xie, Hongling Zhang, Zhoujie Li, Yuanyuan Li, Suming Yang, Meiling Zhang, Wen Li, Jinsong Li

“…Strikingly, diploid ESCs derived from the fusion of PG-haESCs with 7 maternal DMR deletions and AG-haESCs with 2 paternal DMR deletions and maternal Snrpn-DMR deletion also support full-term embryonic development. Moreover, embryos reconstructed by injection of AG-haESCs with hypomethylated H19-DMR into oocytes with H19-DMR deletion develop into live mice sustaining inverted allelic gene expression. …”
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Preimplantation genetic testing for aneuploidy in severe male factor infertility: protocol for a multicenter randomised controlled trial by Li Wang, Li Jin, Yao Lu, Chen Zhang, He-Feng Huang, Yun Sun, Xian-hua Lin, Meng-xi Guo, Dan-dan Wu, Jian-lin Zhang, Cheng-liang Zhou, Chen-ming Xu, Song-chang Chen, Song-ying Zhang, Xiao-xi Sun, Yan-ting Wu

“…Secondary outcomes will be embryo implantation rate, biochemical pregnancy rate, clinical pregnancy rate, spontaneous abortion rate, ongoing pregnancy rate, preterm birth rate, fetal chromosomal abnormality rate, birth defect rate and treatment complications. …”
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Impact of sperm DNA fragmentation index on assisted reproductive outcomes: a retrospective analysis by Bin Yang, Leizhen Xia, Rufei Deng, Liping Wu, Zhiqin Zhang, Xingwu Wu, Tao Ding, Yan Zhao, Jialyu Huang, Jialyu Huang, Zhihui Huang

“…High DFI affected the blastocyst formation rate (56.44%, 55.32%, 53.72%, respectively; P=0.045) and the rate of transferable embryos (3.97 ± 2.71, 3.90 ± 2.7, 3.38 ± 2.4, respectively; P<0.001); however, no significant difference in pregnancy outcomes was observed among the three groups. …”
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Endothelial calcium firing mediates the extravasation of metastatic tumor cells by Marina Peralta, Amandine Dupas, Annabel Larnicol, Olivier Lefebvre, Ruchi Goswami, Tristan Stemmelen, Anne Molitor, Raphael Carapito, Salvatore Girardo, Naël Osmani, Jacky G. Goetz

“…Using high-resolution intravital imaging in zebrafish embryos, we demonstrate that the actomyosin network of ECs controls tissue remodeling and subsequent TC extravasation. …”
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Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish by . Alimuddin, G. Yoshizaki, O. Carman, T. Takeuchi

“…Each of the constructs in circular DNA form was microinjected into 1-cell stage embryos at a concentration of 30mg/ml. Transgenic individuals were identified by polymerase chain reaction (PCR) and their expression levels were analyzed by reverse transcription PCR.  …”
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PATL2 mutations affect human oocyte maternal mRNA homeostasis and protein interactions in cell cycle regulation by Yin-Li Zhang, Zhanhong Hu, Huifang Jiang, Jiamin Jin, Yan Zhou, Mengru Lai, Peipei Ren, Siya Liu, Ying-Yi Zhang, Yan Rong, Wei Zheng, Shen Zhang, Xiaomei Tong, Songying Zhang

“…RNA sequencing revealed that PATL2 mutations decreased mRNA storage in human germinal vesicle (GV) oocytes and impeded mRNA decay during maturation and in early embryos. We demonstrate that PATL2 interacts with CPEB1 and TUT7 in human oocytes to maintain mRNA homeostasis. …”
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Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro by Lixiang Liu, Lixiang Liu, Yu Tang, Yu Tang, Jing Shao, Jing Shao, Bingfeng Fan, Bingfeng Fan, Yifeng Yang, Yifeng Yang, Ying Zhang, Ying Zhang, Xiangyuan Zhao, Xiangyuan Zhao, Hailong Xue, Hailong Xue, Huimin Sun, Huimin Sun, Xulin Zhang, Xulin Zhang, Yushi Zhang, Yushi Zhang, Baozeng Xu, Baozeng Xu

“…IntroductionEarly preimplantation embryos of mammals exhibit pronounced sensitivity to hyperosmotic conditions, which results in an embryonic developmental block. …”
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Effect of exposure to environmental phenols and parabens on folate concentrations among 3–19 years old children and adolescents: A cross-sectional study in NHANES 2005–2016 by Meng Ni, Zhenying Lin, Ze Chen, Ziwen Xu, Qianqian Zhang, Jiuru Zhao, Wei Li, Yanan Tang, Zelin Cao, Baihe Li, Dongting Yao, Chunyu Cheng, Yi Hu, Xiaorui Liu, Jiji Chen, Zhiwei Liu

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Study on the Imprinting Status of Insulin-Like Growth Factor II (IGF-II) Gene in Villus during 6–10 Gestational Weeks by Jianhong Chen, Qun Fang, Baojiang Chen, Yi Zhou, Yanmin Luo

“…To compare the difference of imprinting status of insulin-like growth factor II (IGF-II) gene in villus between normal embryo development group and abnormal embryo development group and to investigate the relationship between karyotype and the imprinting status of IGF-II gene. …”
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β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway by Yu Cai, Hua Yang, Hui Xu, Shanglai Li, Bingru Zhao, Zhibo Wang, Xiaolei Yao, Feng Wang, Yanli Zhang

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Seed Morphometry of Native Indonesian Orchids in the Genus Dendrobium by Sucipto Hariyanto, Intan Ayu Pratiwi, Edy Setiti Wida Utami

“…All the presently investigated seeds showed are transparent with visible embryo and remarkable embryo color variations (such as pale yellow, light yellow, shiny yellow to yellow, orange, and white). …”
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IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice by Tingwenyi Hu, Xiangrong Tang, Tiechao Ruan, Shunhua Long, Guicen Liu, Jing Ma, Xueqi Li, Ruoxuan Zhang, Guoning Huang, Ying Shen, Tingting Lin

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Evaluation of superovulation responses in some domestic ewe breeds by Ayşe Merve Köse, Sakine Ülküm Çizmeci, İrfan Tur, Mehmet Güler

“…Minimum fertilization (embryo) and recovery rates (embryo + UFO) were 55% and 68% for Daglic, 32% and 63% for Herik, and 32% and 62% for Norduz, respectively. …”
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Successful Same-Cycle Blastocyst Transfer following Laparoscopic Ovarian Detorsion: A Report of Two Cases and Literature Review by Mohamad Irani, Reshef Tal, David B. Seifer, Richard V. Grazi

“…Moreover, no cases of ovarian torsion occurring following oocyte retrieval but prior to same-cycle embryo transfer have been reported. Such cases present a clinical dilemma with respect to optimal timing of embryo transfer. …”
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Reproductive Technologies and Genomic Selection in Cattle by Patrice Humblot, Daniel Le Bourhis, Sebastien Fritz, Jean Jacques Colleau, Cyril Gonzalez, Catherine Guyader Joly, Alain Malafosse, Yvan Heyman, Yves Amigues, Michel Tissier, Claire Ponsart

“…In this context, the value of the reproductive techniques including assisted embryo based reproductive technologies (Multiple Ovaluation Embryo Transfer and Ovum pick up associated to in vitro Fertilization) is also revisited. …”
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Sinensetin attenuates hepatic ischemia-reperfusion injury through suppressing GRP78/CHOP-mediated endoplasmic reticulum (ER) stress in mice by Yihong Yang, Guanghua Xiong, Huangqi Shi, Yulin Peng, Jinghong Liu, Yaxuan Jiang, Min Lu, Hongbo Liu, Yong Liu

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Relationship between in ovo feeding and eggshell temperature of breeder eggs during incubation by Maxwell Ansong Okai, Maxwell Ansong Okai, Francis Kruenti, Jacob Alhassan Hamidu, Kokou Tona, Lin Hai

“…The hatching egg biologically provides protection and nutrition for its embryo during the pre-incubation and incubation periods. …”
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Effect of partial oocyte maturation before ovum pick up on developmental competence of buffalo oocytes by Amjad Riaz, Muhammad K. Ashraf, Talha Ashraf, Ali Husnain, Muhammad Yaseen, Muhammad H. Mushtaq, Burhan E. Azam

“…Similarly, oocytes treated with hCG had greater (p = 0.04) development from cultured oocytes to Day 7 embryo (52.5 vs. 18.1 ± 10.3%). Treatment with hCG did not affect the development of cleaved oocytes to Day 7 embryo. …”
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