Assessing molecular changes underlying isopropylated phenyl phosphate (IPP)-induced larval sensorimotor response deficits in zebrafish by Sunil Sharma, Alfredo Rojas, Abhishek Gour, Rosemaria Serradimigni, Connor Leong, Abhisheak Sharma, Subham Dasgupta

“…Within this study, we used zebrafish embryos to systemically study these biological targets. …”
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Evaluation of developmental competence of Sus scrofa domesticus (L.) oocyte-cumulus complexes after intra- and extraovarian vitrification by T. I. Kuzmina, I. V. Chistyakova

“…The methods of fertilization and embryo culture are presented in the guidelines developed by us. …”
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Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene by L. A. Elkonin, N. V. Borisenko, T. E. Pylaev, O. A. Kenzhegulov, S. Kh. Sarsenova, N. Yu. Selivanov, V. M. Panin

“…Using Agrobacterium-mediated genetic transformation of immature embryos of the grain sorghum cv. Avans we have obtained a mutant with improved digestibility of endosperm proteins (up to 92 %) carrying a genetic construct for RNA silencing of the gKAF1 gene. …”
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Controlled ovarian hyperstimulation with or without letrozole for fertility preservation in breast cancer patients: study protocol for a randomised controlled trial by Ping Li, Kun Wang, Qi Fan, Peng Sun, Yuhua Shi, Yanqiu Xie, Weifen Deng, Jiajing Kang

“…The standard options for fertility preservation are oocyte and embryo cryopreservation; both require controlled ovarian hyperstimulation (COH). …”
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Comparison of Endocrine Profile and In Vitro Fertilization Outcome in Patients with PCOS, Ovulatory PCO, or Normal Ovaries by Yi-Ping Zhong, Ying Ying, Hai-Tao Wu, Can-Quan Zhou, Yan-Wen Xu, Qiong Wang, Jie Li, Xiao-Ting Shen, Jin Li

“…The basic clinical features and in vitro fertilization and embryo transfer outcome in patients receiving IVF or intracytoplasmic sperm injection (ICSI) were retrospectively analyzed. …”
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Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes by Hope M Healey, Hayden B Penn, Clayton M Small, Susan Bassham, Vithika Goyal, Micah A Woods, William A Cresko

“…We created a single-cell RNA sequencing atlas of Gulf pipefish embryos to understand the developmental basis of four traits: derived head shape, toothlessness, dermal armor, and male pregnancy. …”
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In Vitro and In Ovo Evaluation of <i>Oenothera biennis</i> L. Oil as an Alternative Preservative for Oil-Based Products by Ramona Fecker, Ștefana Avram, Ileana Cocan, Ersilia Alexa, Larisa Bora, Daliana Minda, Ioana Zinuca Magyari-Pavel, Cristina Adriana Dehelean, Corina Danciu

“…Various methods were applied to assess oxidative stability by calculating the peroxide value, the <i>p</i>-anisidine value, and the total oxidation value, while biological safety was evaluated using the chick embryo’s chorioallantoic membrane and histological analysis. …”
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The potential for gene-editing to increase muscle growth in pigs: experiences with editing myostatin by A. C. Dilger, X. Chen, L. T. Honegger, B. M. Marron, J. E. Beever

“…One male and one female cell line with a deletion of one thymidine residue (− 1 T) were selected for somatic cell nuclear transfer. Of the ~ 900 embryos transferred, there were 12 live births (1 male, 11 females) but only 5 female pigs survived to sexual maturity. …”
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Epidemic Zika virus strains from the Asian lineage induce an attenuated fetal brain pathogenicity by Maïlis Darmuzey, Franck Touret, Emily Slowikowski, Ivan Gladwyn-Ng, Karan Ahuja, Lorena Sanchez-Felipe, Xavier de Lamballerie, Catherine Verfaillie, Pedro E. Marques, Johan Neyts, Suzanne J. F. Kaptein

“…All studied Asian ZIKV strains are neurovirulent, but pre-epidemic strains are consistently more pathogenic in the embryos than their epidemic equivalents. Pathogenicity is not directly linked to viral replication. …”
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BIOTECHNOLOGICAL SYSTEM FOR THE SEARCH OF SUBSTANCES WITH POTENTIAL ACTIVITY AGAINST CORONAVIRUS by M.P. Smetiukh, O.P. Trokhimenko, S.O. Soloviov, I.V. Dziublyk, O.A. Kamatskyi, I.V. Savchuk, N.A. Bobyr

“…The use of intermediate CEF and CEF cell cultures facilitated the gradual adaptation of the virus to the new cultivation conditions due to the antigenic affinity between chicken embryo fibroblast cells and avian embryos. Conclusions: As a result of the conducted research, the vaccine virus IBV H-120 was successfully adapted to cultivation conditions in ВНК-21 cell cultures, using primary trypsinized chicken embryo fibroblast cells as an intermediate system. …”
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Three-dimensional diffractive acoustic tomography by Luca Menozzi, Tri Vu, Aidan J. Canning, Harshal Rawtani, Carlos Taboada, Marie Elise Abi Antoun, Chenshuo Ma, Jesse Delia, Van Tu Nguyen, Soon-Woo Cho, Jianing Chen, Theresa Charity, Yirui Xu, Phuong Tran, Jun Xia, Gregory M. Palmer, Tuan Vo-Dinh, Liping Feng, Junjie Yao

“…Using 3D-DAT on small animal models, we mapped the distribution of the biliverdin-binding serpin complex in glassfrogs, tracked gold nanoparticle accumulation in a mouse tumor model, imaged genetically-encoded photoswitchable tumors, and investigated polyfluoroalkyl substances exposure on developing embryos. With its enhanced imaging performance and high accessibility, 3D-DAT may find broad applications in fundamental life sciences and biomedical research.…”
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Ochratoxin A induces immunotoxicity by targeting Annexin A1 mediated neutrophil apoptosis in zebrafish by Yihong Zheng, Yinuo Liu, Jin Tian, Shuhong Liu, Gaowei Ma, Yupeng Xie, Chenhua Zheng, Zekai Wu

“…However, limited understanding exists regarding its immunotoxicity and the underlying mechanisms, particularly in relation to innate immunity.MethodsZebrafish embryos were exposed to varying concentrations of OTA to assess its impact on embryonic development, innate immune cell formation, and immune response. …”
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Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens by Changjing Zhao, Cheng Yuan, Qingsen Zhao, Aihong Xia, Yaqing Dong, Shuying Xu, Shihan Chen, Yue Yuan, Yongjuan Wang

“…It was subsequently applied to the identification of purposefully randomly mixed infections of two, three, four or five IBV strains mentioned above in samples of chicken embryos. All of the intentionally infected samples showed the expected amplified bands. …”
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Breeding for sustainability: how reproductive biotechnologies can help buffalo farmers combat climate change by Pietro Sampaio Baruselli, Laís Ângelo de Abreu, Vanessa Romário de Paula, Sofía Albertini, Guilherme Felipe Ferreira dos Santos, Lígia Mattos Rebeis, Emanuelle Almeida Gricio, Nelcio A.T. de Carvalho, Otavio Bernardes

“…Additionally, it allows the initiation of superstimulatory treatments at a self-appointed time, providing opportunities to superstimulate buffaloe donors associated with ovum pick-up (OPU) and in vitro embryo production (IVEP). Furthermore, it allows fixed-time embryo transfer in recipients, with high efficiency and no need for estrus detection. …”
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Molecular identification of Newcastle disease virus isolated on the poultry farm of the Moscow Oblast in summer of 2022 by А. А. Treshchalina, А. А. Rtishchev, Е. Yu. Shustova, А. V. Belyakova, A. S. Gambaryan, Е. Yu. Boravleva

“…It was found that the average death time of developing chicken embryos, infected with a minimum infectious dose, was 52 hours, which is typical for the velogenic pathotype. …”
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Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease by S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina

“…In the present study, an evaluation of in vitro regeneration efciency of immature embryos of ten Siberian barley cultivars revealed that only one of these is on a par with the experimental standard cultivar Golden Promise. …”
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Prediction of Reproductive Outcomes of Intracytoplasmic Sperm Injection Cycles Using a Multivariate Scoring System by Ahmed Abuelsoud Abden, Momen Ahmed Kamel, Ahmed Nabil Fetih, Ali Haroun Yousef

“…Women who have a low score have a high chance of having frozen embryos. Likewise, women who have a high score have a very high chance of cycle cancellation. …”
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Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis by K. T. Sultankulova, O. V. Chervyakova, N. S. Kozhabergenov, K. A. Shorayeva, V. M. Strochkov, M. B. Orynbayev, N. T. Sandybayev, A. R. Sansyzbay, A. V. Vasin

“…The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. …”
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Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress by Na Wang, Han Yang, Yelei Chen, Hekun Wang, Chaorui Wang, Jianglin Fan, Yajie Chen, Yinghua Li, Maobi Zhu

“…This is the first study to report that FMN supplementation benefits the in vitro culture of oocytes and early embryo development, potentially by regulating oxidative stress, mitochondrial function, and autophagy.…”
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Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s acti... by Manickam Rajkumar, S. I. Davis Presley, Nathiya Thiyagarajulu, Koyeli Girigoswami, Gopalarethinam Janani, Chinnaperumal Kamaraj, Bharathi Madheswaran, Bhupendra Prajapati, Nemat Ali, Mohammad Rashid Khan

“…In vivo studies of zebrafish embryos showed that Ge/PLA/AuNCs have excellent biocompatibility and nontoxicity. …”
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