Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

“…Here, we present a protocol to identify the overall phenotype by overlaying images of lymphatic vessels acquired from multiple zebrafish embryos of a genetic background. We describe steps for preparing samples, obtaining confocal images, and their computational alignment. …”
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Cardiac Expression of Tnnt1 Requires the GATA4-FOG2 Transcription Complex by Nikolay L. Manuylov, Sergei G. Tevosian

“…Since the myocardial-restricted Fog2 re-expression in the Fog2 null embryos is sufficient to extend their life span, identification of GATA4-FOG2 target genes in cardiomyocytes could shed light on the molecular mechanism of GATA4-FOG2 action in these cells. …”
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Cancer as an Embryological Phenomenon and Its Developmental Pathways: A Hypothesis regarding the Contribution of the Noncanonical Wnt Pathway by Jaime Cofre, Kay Saalfeld, Eliana Abdelhay

“…For gastrulation to occur in human embryos, a mechanism that simultaneously regulates many different processes, such as cell differentiation, proliferation, migration, and invasion, is required to consistently and effectively create a human being during embryonic morphogenesis. …”
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Effects of in-ovo injection of Yu ping feng polysaccharides on growth performance, intestinal development, and immunity in broiler chickens by Li-Gang Xue, Tian-Kui Guo, Juan Wang, Yu-Quan Shan, Li Guo, Dong-Xing Zhang, Zhong Wei, Dan Wang

“…Compared with the other three groups, the in-ovo YPF injection did not affect the hatching rate of chicken embryos; however, the initial body weight of hatchlings significantly increased and the feed conversion ratio decreased. …”
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Can Humanity Thrive Beyond the Galaxy? by Sayaka WAKAYAMA, Teruhiko WAKAYAMA

“…For example, when humans begin living and reproducing in space habitats or on other planets in the solar system, are there risks that future generations may suffer from adverse mutations induced by space radiation, or that embryos and fetuses will develop abnormally in gravitational environments that differ from that of Earth? …”
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Repeatability of Nest Site Selection of Green Turtles on Samandağ Beach by Bektaş Sönmez

“…Sea turtles depend on sandy beaches for nesting, and females perform nest site selection on the beach for the successful development of their embryos. This study investigated the repeatability of nest site selection on Samandağ beach, a major green turtle nesting beach in the Mediterranean. …”
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Application of genome tagging technology in elucidating the function of sperm-specific protein 411 (Ssp411) by Xue-Hai Zhou, Min-Min Hua, Jia-Nan Tang, Bang-Guo Wu, Xue-Mei Wang, Chang-Gen Shi, Yang Yang, Jun Wu, Bin Wu, Bao-Li Zhang, Yi-Si Sun, Tian-Cheng Zhang, Hui-Juan Shi

“…Ssp411 is not detectable in metaphase II (MII) oocytes, zygotes, or 2-cell stage embryos, highlighting its intricate role in early embryonic development. …”
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Determination of the incidence of embryonic death by means of real-time ultrasound scanning in ewes by M. Kemal SARIBAY, Hüseyin ERDEM

“…Of 73 ewes, 60 ewes were pregnant and 79 embryos were detected and embryonic mortality rate was 8.9 % (7 of 79 embriyos) on day 18. …”
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Vertex model with internal dissipation enables sustained flows by Jan Rozman, KVS Chaithanya, Julia M. Yeomans, Rastko Sknepnek

“…Here we extend an active nematic vertex model by replacing substrate friction with internal viscous dissipation, dominant in epithelia not supported by a substrate or the extracellular matrix, which are found in many early-stage embryos. When coupled to cell shape anisotropy, the internal viscous dissipation allows for long-range velocity correlations and thus enables the spontaneous emergence of flows with a large degree of spatiotemporal organisation. …”
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Kank Is an EB1 interacting protein that localises to muscle-tendon attachment sites in Drosophila. by Sara M R Clohisey, Nikola S Dzhindzhev, Hiroyuki Ohkura

“…Kank protein is expressed throughout fly development and increases during embryogenesis. In late embryos, it accumulates to sites of attachment between muscle and epidermal cells. …”
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Fertility Preservation for Cancer Patients: A Review by Tosin Ajala, Junaid Rafi, Peter Larsen-Disney, Richard Howell

“…Oncologists must be aware of situations where their treatment will affect fertility in patients who are being treated for cancer and they must also be aware of the pathways available for procedures such as cryopreservation of gametes and/or embryos. Improved cancer care associated with increased cure rates and long term survival, coupled with advances in fertility treatment means that it is now imperative that fertility preservation is considered as part of the care offered to these patients. …”
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Heterotopic Triplet Pregnancy after In Vitro Fertilization with Favorable Outcome of the Intrauterine Twin Pregnancy Subsequent to Surgical Treatment of the Tubal Pregnancy by Theodoros Felekis, Christodoulos Akrivis, Panagiotis Tsirkas, Ioannis Korkontzelos

“…We describe a case of a woman with heterotopic triplets at 9+4 gestational week following transfer of three embryos obtained by in vitro fertilization techniques. …”
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Protective Effects of Phycobiliproteins from <i>Arthrospira maxima</i> (Spirulina) Against Cyclophosphamide-Induced Embryotoxicity and Genotoxicity in Pregnant CD1 Mice by Yuliana García-Martínez, Amparo Celene Razo-Estrada, Ricardo Pérez-Pastén-Borja, Candelaria Galván-Colorado, Germán Chamorro-Cevallos, José Jorge Chanona-Pérez, Oscar Alberto López-Canales, Hariz Islas-Flores, Salud Pérez-Gutiérrez, Joaquín Cordero-Martínez, José Melesio Cristóbal-Luna

“…<b>Conclusions</b>: Phycobiliproteins from <i>Arthrospira maxima</i> (spirulina) effectively reduced Cp-induced morphological and vascular alterations in embryos and genotoxicity in pregnant mice. These findings highlight their potential as a complementary therapy to mitigate teratogenic risks during chemotherapy. …”
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FERTILITY CARE IN LOW AND MIDDLE INCOME COUNTRIES: Embryologists’ practices of care in IVF-clinics in sub-Saharan Africa by Trudie Gerrits, Andrea Whittaker, Lenore Manderson

“…They enacted care in several ways, towards gametes and embryos, patients, clinics and the profession. These multiple tasks and care practices make for dynamic and fulfilling careers of the interviewed embryologists but also stretch their capacities. …”
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Advancing nuclear transfer cloning in zebrafish (Danio rerio) into a translational pathway using interdisciplinary tools. by Sarah Bodenstein, William Poulos, Fermin Jimenez, Michael Stout, Yue Liu, Zoltan M Varga, Jose Cibelli, Terrence R Tiersch

“…Training, for most steps in the SCNT protocol, could be completed within two months; however, steps that involved micromanipulation of eggs required more than four months of training. Dechorionation of embryos and egg micromanipulation were identified as major constraints because the processes were performed manually and required advanced operator manual skills. …”
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Quantitative perturbation-phenotype maps reveal nonlinear responses underlying robustness of PAR-dependent asymmetric cell division. by Nelio T L Rodrigues, Tom Bland, KangBo Ng, Nisha Hirani, Nathan W Goehring

“…This decoupling helps maintain the embryo along the correct developmental trajectory, thereby ensuring that asymmetric division is robust to perturbation. …”
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Histogenesis of the Oesophagus of Guinea Fowl (Numida meleagris) at Prehatch and Posthatch by Innocent Jonah Gosomji, Sulaiman Olawoye Salami, James Oliver Nzalak, Muhammed Umar Kawu, Emmanuel Vandi Tizhe, Yilzem George Gurumyen, Edward Christopher Dung

“…The histogenesis of the primordial oesophagus was studied to determine the period in which the tunics of the oesophagus developed and became functional in the helmeted guinea fowl (Numida meleagris). Eighteen embryos and nine keets were studied at prehatch and posthatch, respectively. …”
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A chromosome-level genome assembly of wild silkmoth, Bombyx mandarina by Jung Lee, Takashi Kiuchi, Katsushi Yamaguchi, Shuji Shigenobu, Atsushi Toyoda, Toru Shimada

“…The assembly harbors 14,859 protein-coding genes and 628 piRNA clusters across three tissues: ovaries, testis, and embryos. ATAC-seq data comprehensively detected open chromosome regions, which will benefit when CRISPR/Cas9-mediated genome editing is conducted.…”
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Modeling Musculoskeletal Disorders in Zebrafish: Advancements in Muscle and Bone Research by Luca Dalle Carbonare, Michele Braggio, Arianna Minoia, Mattia Cominacini, Maria Grazia Romanelli, João Pessoa, Natascia Tiso, Maria Teresa Valenti

“…Key genes and biological processes in zebrafish that closely mirror those in humans, rapid development, and transparent embryos make zebrafish ideal for the in vivo studies of bone and muscle formation, as well as the molecular mechanisms underlying musculoskeletal disorders. …”
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Interspecific incompatibility in wide hybridization of plants and ways to overcome by L. A. Pershina, N. V. Trubacheeva

“…Early stages of the postzygotic period are crucial for developing hybrid seeds due to embryo’s death, including those associated with uniparental chromosome elimination in hybrid cells and abnormal development of the endosperm. …”
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