High levels of histone acetylation modifications promote the formation of PGCs by Ziduo Zhao, Yuhui Wu, Fufu Cheng, Zhe Wang, Qingqing Geng, Yingjie Niu, Qisheng Zuo, Yani Zhang

“…This study investigates the role of histone acetylation in the differentiation of chicken embryonic stem cells (ESCs) into primordial germ cells (PGCs). …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

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Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines by Ruttachuk Rungsiwiwut, Praewphan Ingrungruanglert, Pranee Numchaisrika, Pramuan Virutamasen, Tatsanee Phermthai, Kamthorn Pruksananonda

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Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage by Francesca Diomede, Thangavelu Soundara Rajan, Valentina Gatta, Marco D’Aurora, Ilaria Merciaro, Marco Marchisio, Aurelio Muttini, Sergio Caputi, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

“…In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer. …”
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The Potential Use of Mesenchymal Stem Cells and Their Derived Exosomes as Immunomodulatory Agents for COVID-19 Patients by Faisal A. Alzahrani, Islam M. Saadeldin, Abrar Ahmad, Dipak Kumar, Esam I. Azhar, Arif Jamal Siddiqui, Bassem Kurdi, Abdulrahim Sajini, Abdulmajeed F. Alrefaei, Sadaf Jahan

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胚胎干细胞体外诱导分化为 CD34+造血前体细胞 by 周其锋, 冯炼强, 李树浓

“…【目的】胚胎干细胞( embryonic stem cell, ES)体外诱导分化,制备CD34+造血前体细胞。【方法】胚胎干细胞在含甲基纤维素培 养液中分化形成胚胎体( embryoid body, EB) ,然后加入造血刺激因子诱导产生 CD34+造血前体细胞,间接免疫荧光及流式细胞仪检测分化结 果。…”
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Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity by Guizhen Du, Mingming Yu, Lingling Wang, Weiyue Hu, Ling Song, Chuncheng Lu, Xinru Wang

“…Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. …”
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Pharmacological or genetic inhibition of LTCC promotes cardiomyocyte proliferation through inhibition of calcineurin activity by Lynn A. C. Devilée, Abou bakr M. Salama, Jessica M. Miller, Janice D. Reid, Qinghui Ou, Nourhan M. Baraka, Kamal Abou Farraj, Madiha Jamal, Yibing Nong, Todd K. Rosengart, Douglas Andres, Jonathan Satin, Tamer M. A. Mohamed, James E. Hudson, Riham R. E. Abouleisa

“…A drug screen targeting proteins involved in CM calcium cycling in human embryonic stem cell-derived cardiac organoids (hCOs) revealed that only the inhibition of L-Type Calcium Channel (LTCC) induced the CM cell cycle. …”
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Characterization of Human Mesenchymal Stem Cells Isolated from the Testis by Letizia De Chiara, Elvira Smeralda Famulari, Sharmila Fagoonee, Saskia K. M. van Daalen, Stefano Buttiglieri, Alberto Revelli, Emanuela Tolosano, Lorenzo Silengo, Ans M. M. van Pelt, Fiorella Altruda

“…Recent gene expression profiling studies have demonstrated that embryonic stem cell- (ESC-) like cells obtained from the human testis are indeed closer to mesenchymal stem cells (MSCs) than to pluripotent stem cells. …”
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Human Motor Neuron Progenitor Transplantation Leads to Endogenous Neuronal Sparing in 3 Models of Motor Neuron Loss by Tanya J. Wyatt, Sharyn L. Rossi, Monica M. Siegenthaler, Jennifer Frame, Rockelle Robles, Gabriel Nistor, Hans S. Keirstead

“…In order to investigate the neurotrophic effects of a motor neuron lineage graft, we transplanted human embryonic stem cell-derived motor neuron progenitors (hMNPs) and examined their histopathological effect in three animal models of motor neuron loss. …”
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Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development by Xiaolei Zhang, Shaorong Gao, Xiaoyu Liu

“…Also, due to the scarcity of human oocytes, low efficiency of blastocyst development and embryonic stem cell line derivation from nuclear transfer embryo (ntESCs), it is far away from the application of this technology on human therapeutics to date. …”
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Pluripotent Stem Cell Metabolism and Mitochondria: Beyond ATP by Jarmon G. Lees, David K. Gardner, Alexandra J. Harvey

“…Metabolism is central to embryonic stem cell (ESC) pluripotency and differentiation, with distinct profiles apparent under different nutrient milieu, and conditions that maintain alternate cell states. …”
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Living biobank: Standardization of organoid construction and challenges by Ruixin Yang, Yao Qi, Xiaoyan Zhang, Hengjun Gao, Yingyan Yu, Yuanyuan Ji

“…Organoids cover two mainstream fields: Adult stem cell-derived organoids (also known as patient-derived organoids) and induced pluripotent stem cell-derived and/or embryonic stem cell-derived organoids. Given the increasing importance of organoids in the development of new drugs, standardized operation, and management in all steps of organoid construction is an important guarantee to ensure the high quality of products. …”
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Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent by Hossein Azizi, Sabine Conrad, Ursula Hinz, Behrouz Asgari, Daniel Nanus, Heike Peterziel, Akbar Hajizadeh Moghaddam, Hossein Baharvand, Thomas Skutella

“…Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age. …”
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Pluripotency Factors on Their Lineage Move by Clair E. Weidgang, Thomas Seufferlein, Alexander Kleger, Martin Mueller

“…Thereby, subsets of pluripotency-maintaining factors have been shown to adopt new roles during lineage specification and have been globally defined towards neuroectodermal and mesendodermal sets of embryonic stem cell genes. However, detailed underlying insights into how these transcription factors orchestrate cell fate decisions remain largely elusive. …”
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The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming by Bharathi Suresh, Junwon Lee, Kye-Seong Kim, Suresh Ramakrishna

“…Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal embryonic stem cell maintenance and differentiation. There are two switches: an E3 ubiquitin ligase enzyme that tags ubiquitin molecules to the target proteins for proteolysis and a second enzyme, the deubiquitinating enzyme (DUBs), that performs the opposite action, thereby preventing proteolysis. …”
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Reprogramming of Melanoma Tumor-Infiltrating Lymphocytes to Induced Pluripotent Stem Cells by Hidehito Saito, Keisuke Okita, Noemi Fusaki, Michael S. Sabel, Alfred E. Chang, Fumito Ito

“…TIL-derived iPSCs display embryonic stem cell-like morphology, have normal karyotype, express stem cell-specific surface antigens and pluripotency-associated transcription factors, and have the capacity to differentiate in vitro and in vivo. …”
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TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6 by Sheng Luo, Wenjin Wang, Jingfang Feng, Rui Li

“…As a novel core part of pluripotency circuitry, testicular expression 10 (TEX10) plays an actively noticeable effect on reprogramming, early embryo development, and embryonic stem cell self-renewal. Nevertheless, TEX10 expressions and functions within bladder carcinoma are still not known. …”
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Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells by Salam Salloum-Asfar, Rudolf Engelke, Hanaa Mousa, Neha Goswami, I. Richard Thompson, Freshteh Palangi, Kamal Kamal, Muna N. Al-Noubi, Frank Schmidt, Sara A. Abdulla, Mohamed M. Emara

“…Other pluripotent stem cell lines (hiPSCs/CRTD5 and hESCs/H9 (human embryonic stem cell line)) as well as nonpluripotent cell lines (BHK-21 and MCF-7) were used to confirm this phenomenon. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…We developed HIOs from the human embryonic stem cell line, H1, and examined the expression of mesenchymal markers during their maturation process. …”
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