KMT2C/KMT2D-dependent H3K4me1 mediates changes in DNA replication timing and origin activity during a cell fate transition by Deniz Gökbuget, Liana Goehring, Ryan M. Boileau, Kayla Lenshoek, Tony T. Huang, Robert Blelloch

“…Using machine learning, we quantify 21 chromatin features predicting local RT and RT changes during differentiation in embryonic stem cells (ESCs). About one-third of the genome shows RT changes during differentiation. …”
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Single-Cell RNA Sequencing Reveals LEF1-Driven Wnt Pathway Activation as a Shared Oncogenic Program in Hepatoblastoma and Medulloblastoma by Christophe Desterke, Yuanji Fu, Jenny Bonifacio-Mundaca, Claudia Monge, Pascal Pineau, Jorge Mata-Garrido, Raquel Francés

“…The analysis of LEF1 genome binding occupancy in H1 embryonic stem cells identified 141 LEF1 proximal targets activated in WNT medulloblastoma, 13 of which are involved in Wnt pathway regulation: <i>RNF43</i>, <i>LEF1</i>, <i>NKD1</i>, <i>AXIN2</i>, <i>DKK4</i>, <i>DKK1</i>, <i>LGR6</i>, <i>FGFR2</i>, <i>NXN</i>, <i>TCF7L1</i>, <i>STK3</i>, <i>YAP1</i>, and <i>NFATC4</i>. …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

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Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines by Ruttachuk Rungsiwiwut, Praewphan Ingrungruanglert, Pranee Numchaisrika, Pramuan Virutamasen, Tatsanee Phermthai, Kamthorn Pruksananonda

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Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage by Francesca Diomede, Thangavelu Soundara Rajan, Valentina Gatta, Marco D’Aurora, Ilaria Merciaro, Marco Marchisio, Aurelio Muttini, Sergio Caputi, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

“…In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer. …”
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The Potential Use of Mesenchymal Stem Cells and Their Derived Exosomes as Immunomodulatory Agents for COVID-19 Patients by Faisal A. Alzahrani, Islam M. Saadeldin, Abrar Ahmad, Dipak Kumar, Esam I. Azhar, Arif Jamal Siddiqui, Bassem Kurdi, Abdulrahim Sajini, Abdulmajeed F. Alrefaei, Sadaf Jahan

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Transcriptome and DNA Methylome Dynamics during Triclosan-Induced Cardiomyocyte Differentiation Toxicity by Guizhen Du, Mingming Yu, Lingling Wang, Weiyue Hu, Ling Song, Chuncheng Lu, Xinru Wang

“…Here, we used human embryonic stem cell- (hESC-) derived cardiomyocytes (CMs) to determine the effects of TCS exposure on cardiac development. …”
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Characterization of Human Mesenchymal Stem Cells Isolated from the Testis by Letizia De Chiara, Elvira Smeralda Famulari, Sharmila Fagoonee, Saskia K. M. van Daalen, Stefano Buttiglieri, Alberto Revelli, Emanuela Tolosano, Lorenzo Silengo, Ans M. M. van Pelt, Fiorella Altruda

“…Recent gene expression profiling studies have demonstrated that embryonic stem cell- (ESC-) like cells obtained from the human testis are indeed closer to mesenchymal stem cells (MSCs) than to pluripotent stem cells. …”
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Human Motor Neuron Progenitor Transplantation Leads to Endogenous Neuronal Sparing in 3 Models of Motor Neuron Loss by Tanya J. Wyatt, Sharyn L. Rossi, Monica M. Siegenthaler, Jennifer Frame, Rockelle Robles, Gabriel Nistor, Hans S. Keirstead

“…In order to investigate the neurotrophic effects of a motor neuron lineage graft, we transplanted human embryonic stem cell-derived motor neuron progenitors (hMNPs) and examined their histopathological effect in three animal models of motor neuron loss. …”
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Pluripotent Stem Cell Metabolism and Mitochondria: Beyond ATP by Jarmon G. Lees, David K. Gardner, Alexandra J. Harvey

“…Metabolism is central to embryonic stem cell (ESC) pluripotency and differentiation, with distinct profiles apparent under different nutrient milieu, and conditions that maintain alternate cell states. …”
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Pluripotency Factors on Their Lineage Move by Clair E. Weidgang, Thomas Seufferlein, Alexander Kleger, Martin Mueller

“…Thereby, subsets of pluripotency-maintaining factors have been shown to adopt new roles during lineage specification and have been globally defined towards neuroectodermal and mesendodermal sets of embryonic stem cell genes. However, detailed underlying insights into how these transcription factors orchestrate cell fate decisions remain largely elusive. …”
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Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent by Hossein Azizi, Sabine Conrad, Ursula Hinz, Behrouz Asgari, Daniel Nanus, Heike Peterziel, Akbar Hajizadeh Moghaddam, Hossein Baharvand, Thomas Skutella

“…Here, we aimed to answer important and fundamental questions in germ cell biology with special focus on the age of the male donor cells and the possibility to generate embryonic stem cell- (ESC-) like cells. While it is believed that spermatogonial stem cells (SSCs) and truly pluripotent ESC-like cells can be isolated from adult mice, it remained unknown if the spontaneous conversion of SSCs to ESC-like cells fails at some age. …”
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The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming by Bharathi Suresh, Junwon Lee, Kye-Seong Kim, Suresh Ramakrishna

“…Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal embryonic stem cell maintenance and differentiation. There are two switches: an E3 ubiquitin ligase enzyme that tags ubiquitin molecules to the target proteins for proteolysis and a second enzyme, the deubiquitinating enzyme (DUBs), that performs the opposite action, thereby preventing proteolysis. …”
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Reprogramming of Melanoma Tumor-Infiltrating Lymphocytes to Induced Pluripotent Stem Cells by Hidehito Saito, Keisuke Okita, Noemi Fusaki, Michael S. Sabel, Alfred E. Chang, Fumito Ito

“…TIL-derived iPSCs display embryonic stem cell-like morphology, have normal karyotype, express stem cell-specific surface antigens and pluripotency-associated transcription factors, and have the capacity to differentiate in vitro and in vivo. …”
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TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6 by Sheng Luo, Wenjin Wang, Jingfang Feng, Rui Li

“…As a novel core part of pluripotency circuitry, testicular expression 10 (TEX10) plays an actively noticeable effect on reprogramming, early embryo development, and embryonic stem cell self-renewal. Nevertheless, TEX10 expressions and functions within bladder carcinoma are still not known. …”
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Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells by Salam Salloum-Asfar, Rudolf Engelke, Hanaa Mousa, Neha Goswami, I. Richard Thompson, Freshteh Palangi, Kamal Kamal, Muna N. Al-Noubi, Frank Schmidt, Sara A. Abdulla, Mohamed M. Emara

“…Other pluripotent stem cell lines (hiPSCs/CRTD5 and hESCs/H9 (human embryonic stem cell line)) as well as nonpluripotent cell lines (BHK-21 and MCF-7) were used to confirm this phenomenon. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…We developed HIOs from the human embryonic stem cell line, H1, and examined the expression of mesenchymal markers during their maturation process. …”
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Causal Association Between Obstructive Sleep Apnea and Migraine: A Bidirectional Mendelian Randomization Study by Wang YP, Wei HX, Hu YY, Zhang C, Niu YM

“…Yu-Pei Wang,1,&ast; Hui-Xia Wei,2,&ast; Yuan-Yuan Hu,3 Chao Zhang,4 Yu-Ming Niu3– 5 1Department of Stomatology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People’s Republic of China; 2Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China; 3Department of Stomatology, Gongli Hospital of Shanghai Pudong New Area, Shanghai, People’s Republic of China; 4Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People’s Republic of China; 5Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei Provincial Clinical Research Center for Umbilical Cord Blood Hematopoietic Stem Cells, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yu-Ming Niu, Department of Stomatology, Gongli Hospital of Shanghai Pudong New Area, Shanghai, People’s Republic of China, Tel +86 13581370999, Email niuyuming@yeah.net Chao Zhang, Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People’s Republic of China, Email zhangchao0803@126.comObjective: Patients with obstructive sleep apnea (OSA) frequently suffer from migraine, however the causal relationship between OSA and migraine is unknown. …”
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miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken by Qingqing Geng, Cai Hu, Ziduo Zhao, Zhe Wang, Fufu Cheng, Jing Chen, Qisheng Zuo, Yani Zhang

“…Overall, our findings suggest that miR-1458 is involved in SSCs formation, which is inhibited by low concentrations of Vitamin B6 and subsequently regulates the formation of SSCs by targeting TBX6, an essential gene involved in embryonic stem cell differentiation. Our study demonstrates the critical role of the Vitamin B6-miR-1458-TBX6 regulatory axis in spermatogonial stem cell formation in Rugao Yellow Chicken, providing new insights into the regulatory mechanisms by which miRNAs affect SSCs formation. …”
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