NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing by Zhongliang Chen, Liangxia Jiang, Min Su, Qibing Zeng, Peng Luo, Liangzhao Chu

“…Here, we show that NLRP7 knockout affects the genetic stability, resulting in increased DNA damage in both human embryonic stem cells and blastoids, making embryonic cells in blastoids more susceptible to apoptosis. …”
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Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice by Tatsuhito Himeno, Hideki Kamiya, Keiko Naruse, Zhao Cheng, Sachiko Ito, Taiga Shibata, Masaki Kondo, Jiro Kato, Tetsuji Okawa, Atsushi Fujiya, Hirohiko Suzuki, Tetsutaro Kito, Yoji Hamada, Yutaka Oiso, Kenichi Isobe, Jiro Nakamura

“…These results suggest that transplantation of angioblast like cells induced from embryonic stem cells appears to be a novel therapeutic strategy for diabetic polyneuropathy.…”
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Vitamin A: A Key Inhibitor of Adipocyte Differentiation by Manal A. Malibary

“…Many recent studies have confirmed the relationship between retinoic acid (RA) and the conversion of embryonic stem cells into adipocytes; however, these studies have shown that RA potently blocks the differentiation of preadipocytes into mature adipocytes. …”
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Highly efficient construction of monkey blastoid capsules from aged somatic cells by Junmo Wu, Tianao Shao, Zengli Tang, Gaojing Liu, Zhuoyao Li, Yuxi Shi, Yu Kang, Jiawei Zuo, Bo Zhao, Guangyu Hu, Jiaqi Liu, Weizhi Ji, Lei Zhang, Yuyu Niu

“…Prior research has predominantly utilized embryonic stem cells. In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. …”
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Asymmetric Cell Kinetics Genes: The Key to Expansion of Adult Stem Cells in Culture by James L. Sherley

“…A singular challenge in stem cell research today is the expansion and propagation of functional adult stem cells. Unlike embryonic stem cells, which are immortal in culture, adult stem cells are notorious for the difficulty encountered when attempts are made to expand them in culture. …”
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Corps embryoïdes : biologie moléculaire et cellules vivantes by Noémie Merleau-Ponty

“…The human embryo must first be inserted into a kinship—then detached from that kinship: one begins humanising it, then halts the humanising process to metamorphose it in a lineage of human embryonic stem cells. Embryoid bodies are constructed laboratory materials that are also steeped in emotions and profit margins, pasts and futures, in the recesses of which beats the organic pulse of the living.…”
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Mitochondrial Dynamics: In Cell Reprogramming as It Is in Cancer by Javier Prieto, Josema Torres

“…Somatic cells can be reprogrammed into a pluripotent cellular state similar to that of embryonic stem cells. Given the significant physiological differences between the somatic and pluripotent cells, cell reprogramming is associated with a profound reorganization of the somatic phenotype at all levels. …”
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Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding by Debora Salerno, Alessandro Rosa

“…Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. …”
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Mesenchymal Stem Cells as a Potent Cell Source for Bone Regeneration by Elham Zomorodian, Mohamadreza Baghaban Eslaminejad

“…A few cell types, including embryonic stem cells (ESCs), adult osteoblast, and adult stem cells, can be used for this purpose. …”
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Current Bioengineering Methods for Whole Kidney Regeneration by Shuichiro Yamanaka, Takashi Yokoo

“…Here, we review recent advances in the field of kidney regeneration, including (i) the directed differentiation of induced pluripotent stem cells/embryonic stem cells into kidney cells; (ii) blastocyst decomplementation; (iii) use of a decellularized cadaveric scaffold; (iv) embryonic organ transplantation; and (v) use of a nephrogenic niche for growing xenoembryos for de novo kidney regeneration from stem cells. …”
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Histone Deacetylase Inhibitors in Cell Pluripotency, Differentiation, and Reprogramming by Androniki Kretsovali, Christiana Hadjimichael, Nikolaos Charmpilas

“…Under distinct developmental conditions, they can promote either self-renewal or differentiation of embryonic stem cells. In addition, they can promote directed differentiation of embryonic and tissue-specific stem cells along the neuronal, cardiomyocytic, and hepatic lineages. …”
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Bone Marrow Stem Cell as a Potential Treatment for Diabetes by Ming Li, Susumu Ikehara

“…Recently, research has focused on stem cells which can be classified into embryonic stem cells (ESCs) and tissue stem cells (TSCs) to generate functional β cells. …”
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New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells by Xiangjun He, Yin-Xiong Li, Bo Feng

“…Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). …”
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Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate by Lijuan Pei, Hongjie Zhang, Meihui Zhang, Yixuan Wang, Ke Wei

“…We utilized a Rcor2-/- mouse embryonic stem cell (mESC) line to investigate the role of Rcor2 in mESC differentiation. …”
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Mesenchymal Stem and Progenitor Cells in Regeneration: Tissue Specificity and Regenerative Potential by Rokhsareh Rohban, Thomas Rudolf Pieber

“…The potential use of embryonic stem cells and invention of induced pluripotent stem cells raised hope for clinical regenerative purposes; however, the use of these interventions for regenerative therapy showed its dark side, as many health concerns and ethical issues arose in terms of using these cells in clinical applications. …”
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Long Noncoding RNA Regulation of Pluripotency by Alessandro Rosa, Monica Ballarino

“…As such, human Embryonic Stem Cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) provide a unique opportunity for studying the earliest steps of human embryogenesis and, at the same time, are of great therapeutic interest. …”
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Generation of live mice from haploid ESCs with germline-DMR deletions or switch by Yongjian Ma, Meng Yan, Zhenfei Xie, Hongling Zhang, Zhoujie Li, Yuanyuan Li, Suming Yang, Meiling Zhang, Wen Li, Jinsong Li

“…Here, we establish a stepwise system to reconstruct a development-related imprinting network, in which diploid embryonic stem cells (ESCs) are derived by fusing between parthenogenetic (PG)- and androgenetic (AG)-haploid embryonic stem cells (haESCs) with different DMR deletions (termed Ha-Ha-fusion system), followed by tetraploid complementation to produce all-haESC fetuses. …”
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Prospect of Human Pluripotent Stem Cell-Derived Neural Crest Stem Cells in Clinical Application by Qian Zhu, Qiqi Lu, Rong Gao, Tong Cao

“…As human pluripotent stem cells including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) can serve as an unlimited cell source to generate NCSCs, hESC/hiPSC-derived NCSCs can be a valuable tool to study the underlying mechanisms of NCSC-associated diseases, which paves the way for future therapies for these abnormalities. …”
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Human epicardial organoids from pluripotent stem cells resemble fetal stage with potential cardiomyocyte- transdifferentiation by Fanwen Wang, Xinle Zou, Huilin Zheng, Tianci Kong, Duanqing Pei

“…Here we use a three-step manipulation of WNT signalling entwined with BMP and RA signalling for generating a self-organized epicardial organoid that highly express with epicardium makers WT1 and TCF21 from human embryonic stem cells. After 8-days treatment of TGF-beta following by bFGF, cells enter into epithelium-mesenchymal transition and give rise to smooth muscle cells. …”
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A biallelically active embryonic enhancer dictates GNAS imprinting through allele-specific conformations by Yorihiro Iwasaki, Monica Reyes, Harald Jüppner, Murat Bastepe

“…Here, we show that the STX16-ICR forms different chromatin conformations with each GNAS parental allele and enhances two GNAS promoters in human embryonic stem cells. When these cells differentiate toward proximal renal tubule cells, STX16-ICR loses its effect, accompanied by a transition to a somatic cell-specific GNAS imprinting status. …”
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