NGS Analysis of Clonality and Minimal Residual Disease in a Patient with Concurrent Richter’s Transformation and CLL/SLL by Shrihari S. Kadkol, Joshua Bland, Ashley Kavanaugh, Hongyu Ni, Vijeyaluxmi Nehru, David Peace

“…Clonality is generally determined by PCR amplification of VDJ rearrangements in the IgH heavy chain or VJ rearrangements in Igκ/Igλ light chain genes followed by capillary electrophoresis. More recently, next-generation sequencing (NGS) has been used to detect clonality in B-cell lymphomas because of the exponential amount of information that is obtained beyond just detecting a clonal population. …”
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Comparison of Deep Learning Techniques in Detection of Sickle Cell Disease. by Mabirizi, Vicent, Kawuma, Simon, Kyarisiima, Addah, Bamutura, David, Atwiine, Barnabas, Nanjebe, Deborah, Oyesigye, Adolf Mukama

“…Recently, the transfer learning technique has proved to be powerful in enhancing the development of deep learning methods for sickle cell disease (SCD) detection as a complement to the clinical method where a hemoglobin electrophoresis machine is used. This is evidenced by some models and algorithms with ≥90% prediction accuracy. …”
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A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy by Jun-Fa Liang, Cheng Peng, Peiyu Li, Qiu-Xiong Ye, Yu Wang, Yun-Ting Yi, Zi-Sheng Yao, Gui-Yun Chen, Bin-Bin Zhang, Jia-Jian Lin, Qizhi Luo, Xuncai Chen

“…Traditional analytical methods, such as liquid chromatography, liquid chromatography mass spectrometry, and capillary electrophoresis, involve time-consuming sample preparation and complicated operation and require expensive instrumentation. …”
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Incidence and Evaluation of Incidental Abnormal Bone Marrow Signal on Magnetic Resonance Imaging by Gunjan L. Shah, Aaron S. Rosenberg, Jamie Jarboe, Andreas Klein, Furha Cossor

“…Twenty two percent underwent some evaluation, most commonly a complete blood count, serum protein electrophoresis, or bone scan. With median follow-up of 41 months, 6% of patients were diagnosed with malignancies including multiple myeloma, non-Hodgkins lymphoma, metastatic non-small cell lung cancer, and metastatic adenocarcinoma. …”
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Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning by P. Janczyk, R. Pieper, V. Urubschurov, K. R. Wendler, W. B. Souffrant

“…Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. Individual microbial communities were identified at each farm. …”
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Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells by Michelle T. Barati, James C. Gould, Sarah A. Salyer, Susan Isaacs, Daniel W. Wilkey, Michael L. Merchant

“…Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose (HG) growth conditions as compared to isoosmotic/normal glucose control (NG⁎) conditions. …”
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Evaluation of DNA Damage in Common Carp (Cyprinus carpio L.) by Comet Assay for Determination of Possible Pollution in Lake Mogan (Ankara) by İsmet Çok, Onur Kenan Ulutas, Öncü Okusluk, Emre Durmaz, Nilsun Demir

“…One of the commonly used techniques to detect DNA damage in aquatic organisms is single-cell gel electrophoresis (comet assay). This study was carried out using Cyprinus carpio in order to identify the possible pollution in Lake Mogan, near Ankara, Turkey, where the city's sewer system and pesticides used in agriculture are believed to be the common causes of pollution. …”
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Characterization of IXINITY® (Trenonacog Alfa), a Recombinant Factor IX with Primary Sequence Corresponding to the Threonine-148 Polymorph by Dougald M. Monroe, Richard J. Jenny, Kevin E. Van Cott, Shelly Buhay, Laura L. Saward

“…The goal of these studies was to extensively characterize the first recombinant FIX therapeutic corresponding to the threonine-148 (Thr-148) polymorph, IXINITY (trenonacog alfa [coagulation factor IX (recombinant)]). Gel electrophoresis, circular dichroism, and gel filtration were used to determine purity and confirm structure. …”
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Phylogenetic Aspects of Antibiotic Resistance and Biofilm Formation of P. aeruginosa Isolated from Clinical Samples by Maryam Motevasel, Masoud Haghkhah, Negar Azimzadeh

“…Fifty-nine P. aeruginosa isolates detected from clinical specimens were used to determine antibiotic susceptibility patterns, prevalence of the genes using PCR, biofilm formation, biofilm eradication concentration assay (MBEC), and epidemiological characteristics using pulsed-field gel electrophoresis (PFGE). Results. The results showed that 35.6% and 16.94% of all the samples were isolated from urine and wounds, 81.33% of the isolates were biofilm producers, 27.11% were multidrug-resistant (MDR), and 100% of the main biofilm former genes belonged to pslA. 94.91% of the isolates possessed brlR and mexA, and 91.5% of them expressed pslA. …”
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Resistance to antibiotics and presence of plasmid in enterobacteria and staphylococcus aureus in the sector of diet of a public hospital by Simone Cardoso Lisboa Pereira, Roberta Silva Ribeiro, Denise Mara Soares Bazzolli, Maria Cristina Dantas Vanetti

“…The susceptibility to antibiotics of therapeutic use was determined by the diffusion technique in agar and plasmidial DNA detection was done by agarose gel electrophoresis. Fourteen genera and 27 species of enterobacteria and others Gram negative bacteria were identified out of 150 isolates obtained. …”
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Unusual Cutaneous Manifestations in a Patient with a History of Hepatitis B: A Case of Scleromyxedema and Literature Review by Gao A, Tian X, Lang D, Chen Y

“…Histopathological examination revealed mucin deposition, fibroblast proliferation, and fibrosis, supporting the diagnosis. Serum protein electrophoresis identified IgG-λ type paraproteinemia. …”
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Proteomic Analysis of Hippocampus and Cortex in Streptozotocin-Induced Diabetic Model Mice Showing Dementia by Kenji Matsuura, Mieko Otani, Masaoki Takano, Keiichi Kadoyama, Shogo Matsuyama

“…The proteomic analysis of the hippocampus and cortex in streptozotocin- (STZ-) treated mouse diabetic model showing dementia was performed using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (n=3/group). …”
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Comprehensive Assessment of Biomolecular Interactions of Morpholine-Based Mixed Ligand Cu(II) and Zn(II) Complexes of 2,2′-Bipyridine as Potential Anticancer and SARS-CoV-2 Agents:... by Karunganathan Sakthikumar, Rui Werner Maçedo Krause, Bienfait Kabuyaya Isamura

“…A new class of pharmacologically active mixed-ligand complexes (1a-2a) [MII(L)2 (bpy)], where L = 2-(4-morpholinobenzylideneamino)phenol), bpy = 2,2′-bipyridine, MII = Cu (1a), and Zn (2a), were assigned an octahedral geometry by analytical and spectral measurements. Gel electrophoresis showed that complex (1a) demonstrated the complete DNA cleavage mediated by H2O2. …”
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Dynamics in the activity of peroxidase and its isoforms in leaves of different apple cultivars by A. E. Mishko, E. O. Lutskiy

“…In the summers of 2018–2019, their leaf samples were analyzed to assess peroxidase activity and its isozyme composition under control and stress conditions. Native electrophoresis in polyacrylamide gel was used for separation of peroxidase isoforms. …”
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Determination of Nicotine in Tobacco by Chemometric Optimization and Cation-Selective Exhaustive Injection in Combination with Sweeping-Micellar Electrokinetic Chromatography by Yi-Hui Lin, Chia-Hsien Feng, Shih-Wei Wang, Po-Yun Ko, Ming-Hsun Lee, Yen-Ling Chen

“…In this paper, an online stacking capillary electrophoresis (CE) method with cation-selective exhaustive injection sweeping-micellar electrokinetic chromatography (CSEI-sweeping-MEKC) is proposed for the optimized analysis of nicotine in tobacco. …”
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Development of a novel, high-throughput imaged capillary isoelectric focusing-Western method to characterize charge heterogeneity of monoclonal antibody heavy and light chains by Gangadhar Dhulipala, Alanna Broszeit, Kun Lu, Nisha Palackal, Erica Pyles

“…Two-dimensional gel electrophoresis has traditionally been used to characterize antibody heavy chain (HC) and light chain (LC) charge variants. …”
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Proteomic Analysis of the Vitreous following Experimental Retinal Detachment in Rabbits by Nakul Mandal, Geoffrey P. Lewis, Steven K. Fisher, Steffen Heegaard, Jan U. Prause, Morten la Cour, Henrik Vorum, Bent Honoré

“…The vitreous samples were evaluated with two-dimensional polyacrylamide gel electrophoresis and the differentially expressed proteins were identified with tandem mass spectrometry. …”
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High-throughput sequence-based microsatellite genotyping for the non-model Neotropical tree species Anadenanthera colubrina (Leguminosae) by Alejandra Lorena Goncalves, María Victoria García, Emilie Chancerel, Olivier Lepais, Myriam Heuertz

“…The main aim was to develop microsatellite markers for sequence-based high-throughput genotyping (SSRseq) in the species and to characterize their information content against traditional capillary electrophoresis-based microsatellite data by estimating the amount of molecularly accessible size homoplasy of each locus. …”
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Increased Small Dense LDL and Decreased Paraoxonase Enzyme Activity Reveals Formation of an Atherogenic Risk in Streptozotocin-Induced Diabetic Guinea Pigs by Mutay Aslan, Filiz Ozcan, Ertan Kucuksayan

“…Paraoxonase activity was measured kinetically by the formation of phenol while LDL subfraction analysis was done by disc polyacrylamide gel electrophoresis. Results. Plasma glucose and high-density lipoprotein (HDL) cholesterol were significantly increased while total cholesterol and LDL cholesterol were significantly decreased in diabetic guinea pigs compared to controls. …”
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YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress by Min Wang, Ping Feng, Xun Chen, Haifang Zhang, Bin Ni, Xiaofang Xie, Hong Du

“…Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. …”
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