Genetic Variation of the DGAT1 gene in Dual-Purpose Dairy Cows and its Influence on Economically Important Breeding Traits by Mark E Armitage, Emma Swan, Denise O'Meara

“…MethodsDNA was extracted from hair samples, and the genotypes of the DGAT1 gene were determined using a combination of conventional PCR, restriction fragment length polymorphism (RFLP), gel electrophoresis, DNA sequencing and novel real-time PCR assays. …”
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Application of Food-Grade Proteolytic Enzyme for the Hydrolysis of Regenerated Silk Fibroin from Bombyx mori by Ji An Joung, Mi Na Park, Ji Young You, Bong Joon Song, Joon Ho Choi

“…The regenerated SF and its enzymatic hydrolysates were characterized by performing UV-visible spectra, gel electrophoresis, and size-exclusion chromatography analyses. …”
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Biochemistry of the Thrombin-Like TLBpic and Its Purification from Bothrops pictus “Jergon de la Costa” (Reptilia: Viperidae) by Victor Ludgardo Álvarez Tohalino, Hugo Guillermo Jiménez Pacheco, Juan Raúl Jesús Zumaran Farfán, Pavel Kewin Delgado Sarmiento, Paulino Rodolfo Zegarra Panca

“…The ESI (electrospray ionisation), corresponding to the electrophoresis of proteins in polyacrylamide gels and to their denaturing solubilization in the presence of the SDS ionic detergent, uniting the proteins, breaking hydrophobic interactions, showing a molecular mass of ∼30 kDa, demonstrating high molecular homogeneity that exists in this family of proteins, is a soft ionization method, in which the samples were ionized by the addition or removal of a proton, with very little extra energy to cause fragmentation of the produced ions. …”
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Polypeptide Expression in Prostate Hyperplasia and Prostate Adenocarcinoma by Ayodele Alaiya, Uwe Roblick, Lars Egevad, Adelaide Carlsson, Bo Franzén, Daniela Volz, Sören Huwendiek, Stig Linder, Gert Auer

“…Cells were collected from prostate hyperplasias (n=6) and prostate carcinomas (n=6) and subjected to two‐dimensional gel electrophoresis (2‐DE). The resulting polypeptide patterns were analysed with the PDQUEST computer software. …”
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Studies of the Antiproliferative Activity of Ruthenium (II) Cyclopentadienyl-Derived Complexes with Nitrogen Coordinated Ligands by Virtudes Moreno, Julia Lorenzo, Francesc X. Aviles, M. Helena Garcia, João P. Ribeiro, Tânia S. Morais, Pedro Florindo, M. Paula Robalo

“…Changes in the tertiary structure of pBR322 plasmid DNA were also observed in gel electrophoresis experiment and the images obtained by atomic force microscopy (AFM) suggest strong interaction with pBR322 plasmid DNA; the observed decreasing of the viscosity with time indicates that the complexes do not intercalate between DNA base pairs. …”
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Occupationally Acquired American Cutaneous Leishmaniasis by Maria Edileuza Felinto de Brito, Maria Sandra Andrade, Éricka Lima de Almeida, Ângela Cristina Rapela Medeiros, Roberto Pereira Werkhäuser, Ana Isabele Freitas de Araújo, Sinval Pinto Brandão-Filho, Alzira Maria Paiva de Almeida, Eduardo Henrique Gomes Rodrigues

“…One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as Leishmania (Viannia) naiffi through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. …”
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Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Ex... by Jian Wang, Bo Xiang, Jixian Deng, Darren H. Freed, Rakesh C. Arora, Ganghong Tian

“…After seven-day exposure to 0.5-Tesla Static Magnetic Field (SMF), Adipose-derived Stem Cells (ASCs) and those labeled by superparamagnetic iron oxide (SPIO) nanoparticles were examined for viability by methyl thiazol tetrazolium (MTT) assay, proliferation by cell counting and bromodeoxyuridine (BrdU) incorporation, DNA integrity by single cell gel electrophoresis, surface antigen by flow cytometry analysis, and the expression of cytokines and genetic markers by reverse transcription-PCR and underwent adipogenic and osteogenic differentiation assessed by quantifying related specific genes expression. …”
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Effect of Selenate on Viability and Selenomethionine Accumulation of Chlorella sorokiniana Grown in Batch Culture by Živan Gojkovic, Carlos Vílchez, Rafael Torronteras, Javier Vigara, Veronica Gómez-Jacinto, Nora Janzer, José-Luis Gómez-Ariza, Ivana Márová, Ines Garbayo

“…Unlike control cultures, SDS PAGE electrophoresis of crude extracts from selenate-exposed cell cultures revealed appearance of a protein band identified as 53 kDa Rubisco large subunit of Chlorella sorokiniana, suggesting that selenate affects expression of the corresponding chloroplast gene as this subunit is encoded in the chloroplast DNA. …”
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Associations of CAG repeat polymorphism in the androgen receptor gene with steroid hormone levels and anthropometrics among men: the role of the ethnic factor by L. V. Osadchuk, G. V. Vasiliev, A. V. Osadchuk

“…Genotyping of the AR CAG repeats was performed using fragment analysis and capillary electrophoresis. Ethnic differences in all anthropometric and hormonal indicators have been established, with higher anthropometric indicators in Slavs than Buryats, and in most cases higher than in Yakuts. …”
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Detection of t(14;16)(q32;q22) and Monosomy 13 by FISH Analysis in a Patient with Multiple Myeloma Associated with Sjögren’s Syndrome: The First Case Report from India by Rupesh R. Sanap, Arundhati S. Athalye, Prochi F. Madon, Boman N. Dhabhar, Mahendra B. Sute, Amit A. Mahabale, Dhanashree J. Warang, Firuza R. Parikh

“…We report a case of a woman diagnosed with Sjögren’s syndrome, who was later suspected to have multiple myeloma on serum protein electrophoresis. Fluorescence in situ hybridization (FISH) was carried out to check for deletions of loci 13q14.3, ATM, p53, and IGH (14q32) rearrangements on a bone marrow aspirate. …”
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Microsatellite loci variability in apple cultivars developed at VIR by A. V. Shlyavas, A. A. Trifonova, I. S. Chepinoga, A. S. Sidnin, K. V. Boris

“…Fluorescently labeled PCR products were separated by capillary electrophoresis on ABI Prism 3130xl.Results. In 35 apple cultivars from VIR, 97 alleles were identified at ten studied loci. …”
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The Characteristic of Biofilm Formation in ESBL-Producing K. pneumoniae Isolates by Xiaofang Gao, Haili Wang, Zhijuan Wu, Pan Sun, Wei Yu, Donghua Chen, Yuhua Mao, Lili Fang, Jia Qian, Li Li, Qian Peng, Yanping Han

“…Thirty ESBL-producing K. pneumoniae isolates from 25 inpatients at Ruijin Hospital, Shanghai, were subjected to pulsed-field gel electrophoresis (PFGE) to estimate genomic relatedness. …”
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Elucidating genetic variability between randomly bred domestic cats and Persian domestic cats from different geographical locations using microsatellite markers by Shirin Mahmoodi, Ali Hojabr Rajeoni, Mehrshad Zeinolabedini, Arash Javanmard, Mohammad Hossein Banabazi

“…DNA extraction was performed using tail hair root, PCR and electrophoresis, and gel staining was performed according to routine laboratory protocol. …”
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Monoclonal Gammopathy of Undetermined Significance with Amyloid Deposition in the Lung and Non-Amyloid Eosinophilic Deposition in the Brain: A Case Report by Francois Abi-Fadel, Nisarg R. Desai, Gita Vatandoust, Rabih Said, Aaron Gottesman, Terenig Terjanian

“…The MGUS was diagnosed 9 months ago on serum protein electrophoresis (SPEP) as patient was referred to the outpatient clinic for hyperglobulinemia on routine blood work. …”
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Analysis of the Muscle Structure and Protein Composition of Different Varieties of Cultured Large Yellow Croaker (Larimichthys crocea) by Yuan MA, Quanyou GUO, Yao ZHENG, Weibo SUN, Liu YANG, Xiaoyan SONG

“…The water-soluble protein content among the five varieties ranged from 58.72 mg/g to 74.85 mg/g, while the electrophoresis results showed no differences in the muscle protein molecular weight distribution across different varieties. …”
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The Construction of Heterothallic Strains of <i>Komagataella kurtzmanii</i> Using the I-SceI Meganuclease by Daria D. Sokolova, Philipp I. Akentyev, Kristina O. Petrova, Lyudmila V. Lyutova, Aleksei A. Korzhenkov, Irek I. Gubaidullin, Stepan V. Toshchakov, Dmitry G. Kozlov

“…This finding was subsequently confirmed by pulsed-field gel electrophoresis of yeast chromosomes. However, the ability of the Y-727-2(alpha)-derived producers to efficiently secrete recombinant β-galactosidase was unaffected by this genomic rearrangement.…”
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Switchable Adhesion of Hydrogels to Plant and Animal Tissues by Leah K. Borden, Morine G. Nader, Faraz A. Burni, Samantha M. Grasso, Irene Orueta‐Ortega, Mahima Srivastava, Paula Montero‐Atienza, Metecan Erdi, Sarah L. Wright, Rajabrata Sarkar, Anthony D. Sandler, Srinivasa R. Raghavan

“…This underscores that EA is mediated by the electrophoresis of chain segments across the gel‐tissue interface.…”
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Seasonal Variations in the Biological Value of Kyrgyz Donkey’s Milk Proteins by Nadira K. Turganbaeva, Mukarama M. Musulmanova, Nurudin A. Kydyraliev

“…The amino acid composition of milk proteins was determined by capillary electrophoresis. The biological value of proteins was assessed by amino acid score, index of essential amino acids (Auxerre index), utility ratio of essential amino acids, and utility ratio of amino acid composition. …”
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Molecular Detection of Bartonella spp. in China and St. Kitts by Ke Huang, Patrick John Kelly, Jilei Zhang, Yi Yang, Weiguo Liu, Anwar Kalalah, Chengming Wang

“…The pan-Bartonella FRET-qPCR is convenient requiring no gel electrophoresis and providing copy numbers, while the gltA-based Bartonella PCR reliably differentiates individual Bartonella species. …”
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Glycosylation Patterns in <i>Meccus (Triatoma) pallidipennis</i> Gut: Implications for the Development of Vector Control Strategies by Elia Torres-Gutiérrez, Frida Noelly Candelas-Otero, Olivia Alicia Reynoso-Ducoing, Berenice González-Rete, Mauro Omar Vences-Blanco, Margarita Cabrera-Bravo, Martha Irene Bucio-Torres, Paz María Silvia Salazar-Schettino

“…Proteins were extracted from each tissue sample and profiled by one- and two-dimensional electrophoresis; protein glycosylation was analyzed by lectin affinity. …”
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