Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples by Ibrahim N. Mwangi, Eric L. Agola, Robert M. Mugambi, Esther A. Shiraho, Gerald M. Mkoji

“…The amplified DNA was either visualized under ultraviolet light after electrophoresis or by directly observing the color change after staining the amplicons with CYBR Green dye. …”
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Study on Management of Blood Transfusion Therapy in Patients with Hereditary Spherocytosis by Shiyue Ma, Lingjian Tang, Chaoli Wu, Hui Tang, Xue Pu, Jinhong Niu

“…Based on the sequencing results, the type of mutated membrane protein in each proband was analyzed using the eosin-5′-maleimide (EMA) binding test and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The hemoglobin level was reduced in all 3 probands after different levels of infection. …”
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Composition and Significance of Bacterial Microbiota and Volatile Organic Compounds of Swiss-Dutch-Type Cheese as Determined by PCR-DGGE and HS-GC by Beata Nalepa, Magdalena Anna Olszewska, Lidia Hanna Markiewicz, Marek Aljewicz

“…Bacterial diversity and VOCs (acetaldehyde; ketones: acetone, diacetyl, acetoin; alcohols: methanol, ethanol; esters: ethyl acetate, ethyl propionate, ethyl butyrate; fatty acids: acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, isocaproic acid, caproic acid, heptanoic acid) were determined by polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE), and headspace gas chromatography (HS-GC), respectively. …”
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Antibiotics Resistance Pattern of Food-Borne Bacteria Isolated from Ice Cream in Bangladesh: A Multidisciplinary Study by Md Sohel, Moushumi Akter, Md. Fahmid Hasan, Shahin Mahmud, Mohammod Johirul Islam, Ashekul Islam, Khairul Islam, Abdullah Al Mamun

“…Kado and Liu method, with some modifications, was used to extract plasmid from the isolated bacteria and visualized through gel electrophoresis. The demographic characteristics showed that the degree of symptoms regarding microorganisms mediated disorders and rate of antibiotics intake in ice cream consumers were significantly higher than the nonconsumers. …”
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Biomorphological and useful agronomic traits of the hexaploid winter triticale cultivar ‘Bilinda’ approved for cultivation in the Northwestern Region of the Russian Federation by L. P. Bekish, V. A. Uspenskaja, T. I. Peneva, N. N. Chikida

“…Conventional techniques were used in the experiments, phenological observations, field and laboratory evaluation tests, and statistical data processing. Electrophoresis of gliadin in the grain of cv. ‘Bilinda’ and its registration as a “protein formula” were performed using the technique developed and approbated by the Molecular Biology Dept. of VIR. …”
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Isolation, Purification and In Vitro Characterization of a Newly Isolated Alkalophilic Phytase Produced by the Halophile <i>Cobetia marina</i> Strain 439 for Use as Animal Food Sup... by Ivanka Boyadzhieva, Kaloyan Berberov, Nikolina Atanasova, Nikolay Krumov, Lyudmila Kabaivanova

“…Its molecular weight was determined to be 43 kDa by gel filtration and 40 kDa by SDS–polyacrylamide gel electrophoresis. The purified enzyme had maximum activity at pH 8.0 and 45 °C, while at 70 °C, it was 80% and about 50% at 80 °C for 40 min, showing its thermostability. …”
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Targeted CQA analytical control strategy for commercial antibody products: Replacing ion-exchange chromatography methods for charge heterogeneity with multi-attribute monitoring by Adam R. Evans, Joseph Mulholland, Michael J. Lewis, Ping Hu

“…Thus, removal of the IEC method from the DP specification was achieved using a validated LC-MS MAM method on a QDa system to directly measure the charge variant PTM CQAs in combination with size exclusion chromatography (SE-HPLC) and capillary electrophoresis (CE-SDS) to measure the non-PTM charge variant CQAs. …”
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Epidemiological Analysis of a <i>K. pneumoniae</i> NDM Outbreak in a Temporary Ward for Patients with Primary COVID-19 Infection by Agata Pruss, Klaudia Miładowska, Helena Masiuk, Paweł Kwiatkowski, Joanna Jursa-Kulesza, Bartosz Wojciuk, Stefania Giedrys-Kalemba, Barbara Dołęgowska

“…The phylogenetic similarity of the obtained strains was examined using pulsed-field electrophoresis. Most strains were isolated from bronchoalveolar lavage. …”
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SSR loci potentially associated with high amylopectine content in maize kernel endosperm by S. I. Vakula, O. A. Orlovskaya, L. V. Khotyleva, A. V. Kilchevsky

“…To this end, a complex of biochemical (calorimetry, bichromate method), molecular-genetic (SSR-PCR, capillary gel electrophoresis with fluorescent detection of fragments) and statistical (descriptive statistics, cluster analysis, χ2) analysis methods was used. …”
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Gastric Precancerous Lesions: From Progenitor Cell and Microsatellite Instability to Clinical Interpretation of Gastric Cancer Risk by A. V. Kononov, V. A. Rubtsov, M. N. Parygina, A. G. Shimanskaya, S. I. Mozgovoi, E. G. Pomorgailo, M. V. Markelova, Yu. A. Fedotova

“…MSI was studied with multiplex PCR evaluation of DNA microsatellites (NR-21, NR-24, NR-27, BAT-25, BAT-26) from paraffin sections, their analysis with capillary electrophoresis. The obtained data were processed with the Statistica 10.0 (StatSoft, USA), presented using descriptive, analytical statistics. …”
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Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening by Channabasavaiah B. Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C. Kent Lloyd

“…Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.…”
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Impact of three different processing methods on the digestibility and allergenicity of Chinese mitten crab (Eriocheir sinensis) tropomyosin by Yufeng Lu, Huafeng Cheng, Shaotong Jiang, Lin Lin, Jianfeng Lu

“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM. …”
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Genome Characteristic of NDM-5-Producing Klebsiella Quasipneumoniae and Klebsiella Pneumoniae in China by Chen L, Liu R, Ye H, Qian J, Lou D

“…Specifically, S1-pulsed-field gel electrophoresis (PFGE), southern blotting, and whole-genome sequencing revealed that ACESH02628 contained an IncHI2/IncHI2A-type plasmid, while ACESH02857 carried an IncX3-type plasmid, both associated with the regional spread of NDM-5. …”
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Development of Fermentation-induced Soymilk Gel: Effects of Different Lactic Acid Bacteria on the Physicochemical Characteristics by Fatemeh Rahmani, Ali Moayedi, Marteza Khomeiri, Mahboobeh Kashiri

“…To understand the effects of acidifying and proteolytic activity of starter culture, syneresis, cell counts, free amino acid content (O-phthalaldehyde method), evaluation of proteolysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and textural parameters of soymilk gels during fermentation were investigated. …”
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Metabolomic profiles of preterm small-for-gestational age infants by Koh Okuda, Nobuhiko Nagano, Kimitaka Nakazaki, Kengo Matsuda, Wataru Tokunaga, Kazumasa Fuwa, Ryoji Aoki, Aya Okahashi, Ichiro Morioka

“…Metabolomic analyses were performed using capillary electrophoresis time-of-flight mass spectrometry. The median GA at birth was not significantly different between the two groups [SGA, 32 (26–36) weeks; non-SGA, 32 (25–35) weeks; p = 0.661)]. …”
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Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer by Christian Arstad, Kristin Taskén, Paulo Refinetti, Ulrika Axcrona, Karl-Erik Giercksky, Per Olaf Ekstrøm

“…From a total number of 1728 microsamples, mitochondrial DNA target sequences were amplified and base substitutions detected by cycling temperature capillary electrophoresis. Real-time PCR was used as a quantitative assay to determine the relative mtDNA copy number of 12 tumors studied, represented by two samples from each (N = 24); a high degree (75%) demonstrated tumor specimen heterogeneity. …”
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High-quality DNA extraction for Desmodium gangeticum: A medicinal shrub of Shivalik Himalayas by Shiwani Singh, Maneesh S. Bhandari, Neeraj Dwivedi

“…Results: With the standardised buffer conditions and protocol, high-quality DNA with purity indicated by a 260/280 nm absorbance ratio in a range of 1.8 to 2.0 with 250 ng/µL – 950 ng/µL concentration from 0.5 g of leaf sample and also validated by agarose gel electrophoresis showing reduced contaminations and intact DNA was isolated. …”
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Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells by Corinne Kai Leng Chew, Hannah O'Toole, Daniela M Zisterer, Seema M. Nathwani, Jade K. Pollock

“…Gene expression was determined using gel electrophoresis and visualised using G:BOX. The intensity of DNA bands for each sample was analysed through gel densitometry. …”
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Profiling Proteins in the Hypothalamus and Hippocampus of a Rat Model of Premenstrual Syndrome Irritability by Mingqi Qiao, Peng Sun, Yang Wang, Sheng Wei, Xia Wei, Chunhong Song, Fushun Wang, Jibiao Wu

“…This study employed two-dimensional gel electrophoresis (2DE) for proteomic map analysis of the hypothalamus and hippocampus of rat models of premenstrual syndrome (PMS) irritability. …”
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Use of Photoreactive Riboflavin and Blue Light Irradiation in Improving Dentin Bonding—Multifaceted Evaluation by Ping-Ju Chen, Jung-Pei Hsieh, Hsiao-Tzu Chang, Yuh-Ling Chen, Shu-Fen Chuang

“…The effects of RF/BL on collagen crosslinking were validated by gel electrophoresis. A nanoindentation test showed that both RF/UVA and RF/BL treatments enhanced the elastic modulus and nanohardness of demineralized dentin. …”
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