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201
Type-Specific Persistence/Clearance Results in Human Papillomavirus Infections in Turkish Women
Published 2021-12-01“…STUDY DESIGN: Data of 115 patients whose human papillomavirus and genotype detection was made with multiplex Polymerase chain reaction, and capillary electrophoresis were categorized as low-risk human papillomavirus/high risk human papillomavirus and single/multiple human papillomavirus infections, and clearance/persistence data of two years were investigated. …”
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202
Composition of high-molecular-weight glutenin subunits and gluten quality in wheat lines with alien genetic material
Published 2024-05-01“…Glutenins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis and HMW-GSs were identified using the Payne nomenclature system. …”
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203
Correlation of precisely fabricated geometric characteristics of DNA-origami nanostructures with their cellular entry in human lens epithelial cells
Published 2025-01-01“…Atomic force microscopy and agarose gel electrophoresis showed that four DONs self-assembled within 3.5h with up to 59% yield and exhibited structural intactness in cell culture medium for 4 h. …”
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204
Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
Published 2018-01-01“…Using single cell gel electrophoresis, a dose-related increase was found for both DNA breaks and oxidative lesions (formamidopyrimidine DNA glycosylase-sensitive sites) upon exposure of hADMSCs to hydrogen peroxide. …”
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205
Preparation of Cod Gelatin Antifreeze Peptides and Its Application of Frozen-Thawed Surimi
Published 2025-02-01“…Additionally, the protective effects of DcAFPs on surimi subjected to repeated freeze-thaw cycles were assessed by measuring myofibrillar protein content and utilizing intrinsic fluorescence spectroscopy and SDS-PAGE gel electrophoresis techniques. The results showed that the optimal preparation process for DcAFPs involved using compound protease, with a substrate concentration of 3.5% (w/v), an enzyme-to-substrate ratio of 5% (w/w), a hydrolysis pH of 8, a hydrolysis temperature of 55 ℃, and a hydrolysis time of 2 hours. …”
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206
Chitosan-DNA nanoparticles: synthesis and optimization for long-term storage and effective delivery
Published 2025-01-01“…Chitosan/DNA complexes remained stable in solution for at least 72 h at a ratio ≥2:1 as assessed by agarose gel electrophoresis. A lower surface charge with lower N:P ratios was indicated by zeta potential measurements. …”
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207
DNA-marker based identification of the RPV3 gene determining downy mildew resistance in grapevines
Published 2018-09-01“…The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. …”
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208
Molecular genetic characteristics of broccoli (Brassica oleracea L. var. italica Plenck) from the VIR collection
Published 2020-10-01“…PCR products were separated by electrophoresis on a 3% agarose gel.Results and conclusions. …”
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209
A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System
Published 2012-12-01“…Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular-weight DNA from both samples was cloned into fosmid vectors. …”
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210
Neutralization by Insulin of the Hypertensive Effect of Dermcidin Isoform 2: An Environmentally Induced Diabetogenic and Hypertensive Protein
Published 2014-01-01“…Dermcidin was prepared by electrophoresis using plasma from the subjects with acute ischemic heart disease. …”
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211
Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells
Published 2017-01-01“…In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. …”
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212
Serum HDL and their subfractions are impaired in multisystem inflammatory syndrome in children (MIS-C)
Published 2025-01-01“…We analyzed serum cholesterol, HDL and HDL-SUB (by capillary electrophoresis) in relation to serum values of biomarkers of inflammation and endothelial damage (by microfluidic immunoassays) in 48 patients with MIS-C at hospital admission and in 48 age- and sex-matched healthy controls. …”
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213
Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor
Published 2023-05-01“…Diameter, assembly process, and morphology of NPs were observed by Dynamic Light Scattering, Native‐Polyacrylamide Gel Electrophoresis, and Atomic Force Microscopy. Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. …”
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214
The Effect of Lipids on the Structure and Function of Egg Proteins in Response to Pasteurization
Published 2025-01-01“…The protein structural changes were analyzed using polypropylene gel electrophoresis, circular dichroism, ultraviolet absorption spectra, and exogenous fluorescence spectra, and the functional properties were assessed through solubility measurements and particle size analysis, while protein antigenicity was evaluated using an enzyme-linked immunosorbent assay. …”
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215
Glucose-6-Phosphate Dehydrogenase Deficiency and Sickle Cell Trait among Prospective Blood Donors: A Cross-Sectional Study in Berekum, Ghana
Published 2016-01-01“…Sickle cell haemoglobin variants were determined using cellulose acetate electrophoresis (pH 8.6). Qualitative G6PD status and quantitative G6PD enzyme activity were determined using methaemoglobin reduction and Trinity Biotech G6PD test kit, respectively. …”
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216
Prevalence and methodologies for detection, characterization and subtyping of Listeria monocytogenes and L. ivanovii in foods and environmental sources
Published 2017-09-01“…In this review, we (A) described L. monocytogenes and L. ivanovii, world-wide incidence of listeriosis, and prevalence of various L. monocytogenes strains in food and environmental sources; (B) comprehensively reviewed different types of traditional and newly developed methodologies, including culture-based, antigen/antibody-based, LOOP-mediated isothermal amplification, matrix-assisted laser desorption ionization-time of flight-mass spectrometry, DNA microarray, and genomic sequencing for detection and characterization of L. monocytogenes in foods and environmental sources; (C) comprehensively summarized different subtyping methodologies, including pulsed-field gel electrophoresis, multi-locus sequence typing, ribotyping, and phage-typing, and whole genomic sequencing etc. for subtyping of L. monocytogenes strains from food and environmental sources; and (D) described the applications of these methodologies in detection and subtyping of L. monocytogenes in foods and food processing facilities.…”
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217
The impact of terahertz radiation on an extremophilic archaean Halorubrum saccharovorum proteome
Published 2017-02-01“…We identified under- or overexpressed proteins in response to terahertz radiation using 2D electrophoresis with subsequent MALDI-TOF mass spectrometry. …”
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218
Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan
Published 2025-01-01“…DNA was extracted from leaves, amplified by PCR and separated by polyacrylamide gel electrophoresis. Statistical analysis included dendrogram construction and principal coordinate analysis (PCoA). …”
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219
Age and diet affect self-resilience of intestinal microbiome in mice
Published 2025-01-01“…In addition, 16S rRNA sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis were performed to identify the bacterial taxa and microbiome structure, respectively.ResultsThe diversity of the gut microbiota in infant mice was recovered by the sixth week, while relative abundance of Ruminococcaceae_UCG_014 was low and did not return to normal levels. …”
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220
A Rapid Screening Method for Sibutramine Hydrochloride in Natural Herbal Medicines and Dietary Supplements
Published 2021-01-01“…While a variety of chromatography- and electrophoresis-based systems have been reported to measure this analyte, they generally suffer from high costs, complicated sample preparation, and a costly analytical infrastructure. …”
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