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181
Changes in wheat protein digestibility and allergenicity: Role of Pediococcus acidilactici XZ31 and yeast during dough fermentation
Published 2023-11-01“…Protein digestibility were evaluated using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and enzyme-linked immunosorbent assay. …”
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182
Flavor release from traditional dry-cured pork during oral processing
Published 2023-01-01“…Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that oral processing has a certain effect on the protein composition and concentration in saliva. …”
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183
Differentiation of Lynch syndrome from other forms of non-polyposis colorectal cancer among Russian patients
Published 2014-08-01“…At its detection search of germline mutations by polymerase chain reaction methods, electrophoresis and direct sequencing was carried out.Results. …”
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184
Spore-Derived Isolates from a Single Basidiocarp of Bioluminescent <i>Omphalotus olivascens</i> Reveal Multifaceted Phenotypic and Physiological Variations
Published 2025-01-01“…At least three separate fluorescent bands were observed by gel electrophoresis from one of the cultures, while only one was observed in others. …”
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185
Zymography of proteases in honey bees (Apis Mellifera) infected with Nosema ceranae
Published 2024-01-01“…Also, the protein pattern of infected honey bee tissue extracts was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Bands with molecular weights of 25, 35, 48, 63, 75, and 100 kDa were observed. …”
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186
Optimization of the DNA isolation procedure for assessing the genetic diversity of essential oil roses (<i>Rosa</i> L.)
Published 2024-10-01“…Effectiveness of the technique was assessed using spectrophotometry, horizontal electrophoresis, and ISSR-PCR. Results. DNA preparations extracted with the modified technique were well visualized on the electropherogram and demonstrated high spectrophotometric values. …”
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187
Effects of Different Heat Treatments on the Structure and Physicochemical Properties of Proteins from Skimmed Camel’s Milk
Published 2024-12-01“…In this study, we investigated the changes of physicochemical properties and structure of defatted camel’s milk proteins with four different heat treatments (65 ℃/30 min (T1), 85 ℃/15 s (T2), 120 ℃/15 s (T3), and 135 ℃/5 s (T4)) by measuring particle size, potential, turbidity, secondary structure, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pattern, tertiary structure, surface hydrophobicity, free sulfhydryl group and disulfide bond content. …”
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188
Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods
Published 2020-01-01“…The multiplex PCR performed on DNA extracted from stool elicited the expected band at 129 bp on gel electrophoresis of the PCR yield providing molecular evidence of intestinal strongyloidiasis. …”
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189
Expression of the Gene Encoding the Brucella abortus BAB1-0278 Antigen in Probiotic Lactococcus lactis Bacteria
Published 2024-10-01“…BAB1-0278 expression was confirmed by reverse polymerase chain reaction (RT-PCR) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot techniques. …”
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190
Cytogenetic impact of gamma radiation and its effects on growth, yield and drought tolerance of maize (Zea mays L.)
Published 2025-02-01“…The study also investigates the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) banding patterns of proteins in grains yielded under the influence of gamma radiation and drought treatments. …”
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191
Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain
Published 2025-01-01“…The partially purified azurin protein was characterized using polyacrylamide gel electrophoresis, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. …”
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192
Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas
Published 2023-01-01“…The isolated RNA was evaluated for concentration and purity by spectrophotometry, for structural and functional integrity by electrophoresis and expression of the RNase P gene, and usefulness in serotype-specific DENV detection by conventional and real-time RT-PCR. …”
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193
Lipid Mediator Informatics and Proteomics in Inflammation-Resolution
Published 2006-01-01“…This review presents detailed protocols used in our lab for LM informatics and proteomics using LC-UV-MS/MS, GC-MS, ELISA, novel databases and searching algorithms, and 2-dimensional gel electrophoresis and LC-nanospray-MS/MS peptide mapping.…”
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194
Betaine Treatment Attenuates Chronic Ethanol-Induced Hepatic Steatosis and Alterations to the Mitochondrial Respiratory Chain Proteome
Published 2012-01-01“…Chronic ethanol ingestion resulted in triglyceride accumulation which was attenuated in the ethanol plus betaine group. Blue native gel electrophoresis (BN-PAGE) revealed significant decreases in the content of the intact oxidative phosphorylation complexes in mitochondria from ethanol-fed animals. …”
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195
Effect of Black Cumin on the Expression of Genes Related to Lipid Metabolism in Adipose Tissue: A Mouse Model Study
Published 2024-02-01“…Total RNA was extracted, and after the qualification by agarose gel electrophoresis, cDNA was synthesized. The expression changes of Leptin, AdipoQ, and PPARG genes were measured by real-time RT-PCR. …”
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196
Binding Investigation of Some Important Metal Ions Copper (I), Nickel (II), and Aluminium (III) with Bovine Serum Albumin Using Valid Spectroscopic Techniques
Published 2023-01-01“…These findings were similar to our previous findings using affinity capillary electrophoresis (ACE). Therefore, it can be inferred that the FT-IR and UV techniques might be utilised effectively to assess the metal-protein interaction and can have wide application in routine analysis. …”
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197
ALKALOHALOPHILIC BACTERIA OF THE FAMILY BACILLACEAE IN THE LAKES OF THE BADAIN JARAN DESERT (CHINA)
Published 2018-05-01“…To study the hydrochemical indicators of water, the following methods were used: atomic emission spectrometry with inductively coupled plasma, ion chromatography and capillary electrophoresis. As a result, it was found that a sodium cation and anions of carbonate, bicarbonate of chlorine and sulphate dominate in the soda-salt lakes of the Badain Jaran desert in a multicomponent composition. …”
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198
Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals
Published 2012-01-01“…The zeta potential of nanoparticle/DNA complexes drops down from +29.4 mV to +23.1 mV comparing with pure nanoparticles. Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease. …”
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199
Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome
Published 2022-07-01“…SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. Using them, the corresponding gene SCA was mapped on Linkage Group V. …”
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200
Isolation and Identification of Some Probiotic Bacteria and Their Potential Role in Improving Immune Response and Resistance of Nile Tilapia (Oreochromis niloticus) in Comparison w...
Published 2020-01-01“…The potential probiotic based on the in vitro antimicrobial activity test was identified as P. putida using routine and gel electrophoresis and 16S rRNA sequencing. During the first and the second month of experiment, there was a highly significant increase in the survival percent of the experimental fish in both treated groups with probiotics. …”
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