Cryo-electron tomography pipeline for plasma membranes by Willy W. Sun, Dennis J. Michalak, Kem A. Sochacki, Prasanthi Kunamaneni, Marco A. Alfonzo-Méndez, Andreas M. Arnold, Marie-Paule Strub, Jenny E. Hinshaw, Justin W. Taraska

“…Abstract Cryo-electron tomography (cryoET) provides sub-nanometer protein structure within the dense cellular environment. …”
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The big chill: Growth of in situ structural biology with cryo-electron tomography by Mikhail Kudryashev

“…In situ structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. …”
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MitoSeg: Mitochondria segmentation tool by Faris Serdar Taşel, Efe Çi̇ftci̇

Subjects: “…Electron tomography…”
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Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure by Ranhao Zhang, Yuan Shen, Xueming Li

“…Summary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. …”
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Cellular electron tomography of the apical complex in the apicomplexan parasite Eimeria tenella shows a highly organised gateway for regulated secretion. by Alana Burrell, Virginia Marugan-Hernandez, Richard Wheeler, Flavia Moreira-Leite, David J P Ferguson, Fiona M Tomley, Sue Vaughan

“…Using a combination of cellular electron tomography and serial block face-scanning electron microscopy (SBF-SEM) we have reconstructed the entire apical end of Eimeria tenella sporozoites; we report a detailed dissection of the three- dimensional organisation of the conoid and show there is high curvature of the tubulin-containing fibres that might be linked to the unusual comma-shaped arrangement of protofilaments. …”
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Ion beam induced secondary electron tomography of acrylonitrile-styrene-acrylate/polycarbonate polymer blends for fused filament fabrication and injection moulding by J. Hernández-Saz, D. Moreno-Sanchez, L. M. Valencia, Y. Gómez, S. I. Molina, M. Herrera

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Rapid structural analysis of bacterial ribosomes in situ by Barrett M. Powell, Tyler S. Brant, Joseph H. Davis, Shyamal Mosalaganti

“…In contrast, analogous structural analysis in cells via cryo-electron tomography (cryo-ET) remains challenging due to critical technical bottlenecks, including low-throughput sample preparation and imaging, and laborious data processing methods. …”
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Size-controlled assembly of phase separated protein condensates with interfacial protein cages by Hyeok Jin Oh, Yongsuk Lee, Haerang Hwang, Kibeom Hong, Hyeongjoo Choi, Jin Young Kang, Yongwon Jung

“…In addition, the 3D structures of intact protein condensates with interfacial cages are visualized with cryo-electron tomography (ET). This work offers a versatile platform for designing size-controlled, surface-engineered protein condensate materials.…”
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Sub-nanometer depth resolution and single dopant visualization achieved by tilt-coupled multislice electron ptychography by Zehao Dong, Yang Zhang, Chun-Chien Chiu, Sicheng Lu, Jianbing Zhang, Yu-Chen Liu, Suya Liu, Jan-Chi Yang, Pu Yu, Yayu Wang, Zhen Chen

“…Attaining better depth resolution often requires large sample tilt angles and numerous projections, as demonstrated in atomic electron tomography. Here, we introduce an extension of multislice electron ptychography, which couples only a few small-angle projections to improve depth resolution by more than threefold, reaching the sub-nanometer scale and potentially approaching the atomic level. …”
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Dynamics of the mammalian pyruvate dehydrogenase complex revealed by in-situ structural analysis by Chen Wang, Cheng Ma, Yuanyou Xu, Shenghai Chang, Hangjun Wu, Chunlan Yan, Jinghua Chen, Yongping Wu, Shaoya An, Jiaqi Xu, Qin Han, Yujie Jiang, Zhinong Jiang, Xiakun Chu, Haichun Gao, Xing Zhang, Yunjie Chang

“…Here, we report the in-situ architecture of intact mammalian PDHc by cryo-electron tomography. The organization of peripheral E1 and E3 components varies substantially among the observed PDHcs, with an average of 21 E1 surrounding each PDHc core, and up to 12 E3 locating primarily along the pentagonal openings. …”
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DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp. by Oleg N. Reva, Violetta La Cono, Laura Marturano, Francesca Crisafi, Francesco Smedile, Manasi Mudaliyar, Debnath Ghosal, Elena A. Selivanova, Marina E. Ignatenko, Manuel Ferrer, Laura Fernandez-Lopez, Mart Krupovic, Michail M. Yakimov

“…Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. …”
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High-resolution Cryo-EM Structure Determination of a-Synuclein—A Prototypical Amyloid Fibril by Juan Sanchez, Joshua Pierson, Collin Borcik, Chad Rienstra, Elizabeth Wright

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Anti-Ebola virus mAb 3A6 protects highly viremic animals from fatal outcome via binding GP(1,2) in a position elevated from the virion membrane by Kathryn M. Hastie, Zhe Li Salie, Zunlong Ke, Peter J. Halfmann, Lisa Evans DeWald, Sara McArdle, Ariadna Grinyó, Edgar Davidson, Sharon L. Schendel, Chitra Hariharan, Michael J. Norris, Xiaoying Yu, Chakravarthy Chennareddy, Xiaoli Xiong, Megan Heinrich, Michael R. Holbrook, Benjamin Doranz, Ian Crozier, Yoshihiro Kawaoka, Luis M. Branco, Jens H. Kuhn, John A. G. Briggs, Gabriella Worwa, Carl W. Davis, Rafi Ahmed, Erica Ollmann Saphire

“…Using X-ray crystallography and cryo-electron tomography of mAb 3A6 complexed with its stalk–MPER epitope, we reveal a previously undescribed mechanism in which 3A6 binds to a conformation of GP1,2 that is lifted from the virion membrane. …”
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A single residue switch mediates the broad neutralization of Rotaviruses by Yang Huang, Feibo Song, Yuanjun Zeng, Hui Sun, Roufang Sheng, Xuechun Wang, Liqin Liu, Guoxing Luo, Yanan Jiang, Yaling Chen, Mengxuan Zhang, Shiyin Zhang, Ying Gu, Hai Yu, Shaowei Li, Tingdong Li, Qingbing Zheng, Shengxiang Ge, Jun Zhang, Ningshao Xia

“…A combination of time-resolved cryo-electron microscopy (cryo-EM) and in situ cryo-electron tomography (cryo-ET) analysis reveals a counterintuitive dynamic process of virus inactivation, in which 7H13 asymmetrically binds to a conserved epitope in the capsid-proximal aspect of VP4, triggers a conformational switch in a critical residue—F418—thereby disrupts the meta-stable conformation of VP4 essential for normal viral infection. …”
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