Decoding circRNA translation: challenges and advances in computational method development by Jingjing Zhang, Jingjing Zhang, Rui Zhou, Rui Zhou, Huiling Zhang, Yin Peng, Jintao Meng, Wenhui Xi, Yanjie Wei

“…Our benchmarking revealed that: (1) cirCodAn achieved superior predictive accuracy while maintaining user accessibility; (2) the training data selection during method development critically impacts model performance. …”
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Antibiotic carry over is a confounding factor for cell-based antimicrobial research applications by Miran Yousri Elfar, Helen Louise Brown, Aled Clayton, Phil Stephens

“…Researchers should carefully consider medium selection and supplementation during method development as accurately determining the antimicrobial mechanisms of any CM is essential for validating future cell-based therapeutic applications.…”
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Latest Advances in Structural Insights and Quantification Techniques for Type I Collagen Biomarkers: A path toward standardization? by Justine Demeuse, Alix Mackowiak, Elodie Grifnée, Philippe Massonnet, Loreen Huyghebaert, Thomas Dubrowski, Stéphanie Peeters, Caroline Le Goff, Etienne Cavalier

“…For accurate assay development, a gold-standard technique like LC-MS/MS is essential, requiring full peptide characterization during method development. This review aims to present recent advances in the characterization of Type I collagen-derived peptides, providing a foundation for improved biomarker standardization and application in clinical practice.…”
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Development and Validation of a Stability-Indicating RP-HPLC Method for Edaravone Quantification by Riuna O’Neill, Okhee Yoo, Philip Burcham, Minh Nguyen, Lee Yong Lim

“…Ten RP-HPLC methods based on the previously published literature were evaluated during method development. The optimal method employed a gradient method on an Agilent ZORBAX Extend-C18 column (150 × 4.6 mm, 5 µm) and produced a sharp and symmetrical drug peak. …”
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A Novel Liquid Chromatography–Tandem Mass Spectrometry Method to Quantify Tryptophan and Its Major Metabolites in Serum to Support Biomarker Studies in Patients with Cancer Undergo... by Anna Siemiątkowska, Barbara Kuźnar-Kamińska, Katarzyna Kosicka-Noworzyń, Kamila Nowaczewska, Hanna Winiarska, Dominika Popiołek, Filip Kamiński, Franciszek K. Główka

“…Although analyzing TRP derivatives in biological matrices is not a new topic, we encountered multiple challenges during method development. One of them was the phenomenon of cross-interferences between the analyzed molecules, which has not been explored in most published papers. …”
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Greenness assessment profile of experimentally designed chromatographic method for the simultaneous analysis of ofloxacin and racecadotril in the presence of racecadotril impuritie... by Nada Nabil, Ghada A. Sedik, Hala E. Zaazaa, Sally S. El-Mosallamy

“…Experimental design using face-centred composite design was utilized during method optimization to obtain the highest level of resolution with the fewest number of experimental trials for the determination of ofloxacin (OFLU) and racecadotril (RAC) in the presence of RAC impurities. …”
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Development of acceptance criteria for the results of anti-D IgG quantification in human anti-D (Rh_о) immunoglobulin preparations by enzyme immunoassay by E. V. Shvedova, S. A. Leshina, E. Yu. Kudasheva, I. V. Borisevich, V. A. Merkulov, D. V. Shvedov

“…It is possible to use the CCDee phenotype, as long as the user adheres to the acceptance criteria for the results, developed during method validation. The aim of this study was to develop the acceptance criteria for the results of anti-D IgG quantification in human anti-D immunoglobulin preparations by the ELISA method that would allow using CCDee erythrocytes to prepare the immunosorbent should there be no erythrocytes of the ccDEE phenotype available. …”
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LC–MS/MS quantification of 20(S)-protopanaxadiol in complex biological matrices for bioanalytical method validation and pharmacokinetic analysis by Pengfei Li, Shanshan Liu, Hongyan Zhuang, Mengxi niu, Fei Pan, Nan Wang, Sha Sha, Qian Wang, Jing Wang

“…A key aspect of our work is the use of a “programmed injection” technique, which significantly reduces the analysis time from 4.2 min during method exploration to 2.4 min. These methods have achieved a relatively low quantification limit of 2.5 ng/mL. …”
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