Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context by Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, Kaixiang Zhang, Jinghong Li

“…The capacity of CRISPR/Cas13a for discriminating single nucleotide variant (SNV) in viral genome is leveraged by designing synthetic mismatches and hairpin structure in CRISPR RNA (crRNA), enabling robust discrimination of different SARS-CoV-2 variants. …”
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Derivation and validation of a prediction model for inadequate bowel preparation in Chinese outpatients by Huifang Yin, Yan Wang, Hangfang Wang, Tian Li, Xiangxiang Xu, Fengyu Li, Lihua Huang

“…The predictive model was constructed based on multivariate logistic regression, and the discrimination and calibration of the prediction model were evaluated via internal and external validation; additionally, a clinical decision curve was drawn to evaluate the clinical utility of the model. …”
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Analyzing the serum biochemical factors that influence early neurological deterioration in ischemic Stroke patients and developing a nomogram prediction model by Xiaoni Zhan, Yunyun Xu, Rongrong Ma

“…Model performance was evaluated using internal validation with the Bootstrap method, assessing discrimination, calibration, and clinical validity. Results: Factors such as history of diabetes, fasting plasma glucose (FBG), triglyceride (TG), homocysteine (Hcy), and C-reactive protein (CRP) were identified as single factors for early functional deterioration in IS patients (P<0.05). …”
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A qualitative study of stigma in patients with low anterior resection syndrome of rectal cancer (直肠癌低位前切除综合征患者病耻感的质性研究) by WEN Ruizhen (温瑞珍), CHEN Piao (陈飘), LI Yalan (李亚兰), CAO Aifang (曹爱芳), WANG Miaolan (王淼兰), ZHENG XiaoXuan (郑筱暄), PENG Hui (彭慧)

“…Theme 1: Stigma experiences, including feelings of shame, discrimination, and loneliness; Theme 2: Determinants of stigma, including disease factors and lack of public knowledge; Theme 3: Stigma sensing approaches, including avoiding social contact, concealing the condition, and actively coping. …”
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Adapting a Mobile Health App for Smoking Cessation in Black Adults With Anxiety Through an Analysis of the Mobile Anxiety Sensitivity Program Proof-of-Concept Trial: Qualitative St... by Marshall K Cheney, Adam C Alexander, Lorra Garey, Matthew W Gallagher, Emily T Hébert, Anka A Vujanovic, Krista M Kezbers, Cameron T Matoska, Michael J Zvolensky, Michael S Businelle

“…MethodsThe MASP smoking cessation app was adapted from an evidence-based app by adding culturally tailored narration and images specific to the Black community, educational content on tobacco use in the Black community and the role of menthol, culturally tailored messages, and addressing tobacco use and racial discrimination. The MASP app was piloted with 24 adults with high anxiety sensitivity who identified as Black, smoked daily, and were not currently using medications or psychotherapy for smoking cessation. …”
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Investigating mental health disparities in rural sexual and gender minority adults: protocol for the rural exploration and approaches to LGBTQ + Mental Health (REALM) prospective c... by Shoshanna L. Fine, Kalai Willis, Iaah L. Lucas, Kirsten F. Siebach, Jennifer L. Glick, Mariah Valentine-Graves, Savannah Winter, Michael Smith, Thomas Waltz, Gina Bailey Herring, Marissa Hannah, Holly C. Wilcox, Travis Sanchez, Stefan D. Baral, Sarah M. Murray

“…Few existing studies, however, have explored how pervasive experiences of SGM-related stigma, discrimination, and trauma (i.e., minority stress) contribute to adverse mental health outcomes among diverse sub-populations of SGM adults living in rural areas of the United States. …”
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The role of the TAPSE/sPAP ratio as a predictor of mortality in Pulmonary Arterial Hypertension: Its value for patient risk stratification by Paul Palacios-Moguel, MD, Guillermo Cueto-Robledo, MD, Héctor González-Pacheco, MD, Jorge Ortega-Hernández, MD, María Berenice Torres-Rojas, MD, Dulce Iliana Navarro-Vergara, MD, Marisol García-Cesar, MD, Cinthia Alejandra González-Nájera, MD, Carlos Alfredo Narváez-Oríani, MD, Julio Sandoval, MD

“…Additional statistical measures, such as the area under the curve (AUC), net reclassification index (NRI), and integrated discrimination improvement, assessed the predictive ability of TAPSE/sPAP in combination with the ESC/ERS risk score, and other risk assessment strategies (COMPERA and Reveal Lite 2). …”
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Proposal for a revised Barthel index classification based on mortality risk assessment in functional dependence for basic activities of daily living by Vicente Martín Moreno, Vicente Martín Moreno, María Inmaculada Martínez Sanz, Amanda Martín Fernández, Sara Guerra Maroto, Eva Sevillano Fuentes, Elena Pérez Rico, Irene Sánchez González, Miriam Fernández Gallardo, Julia Herranz Hernando, María Palma Benítez Calderón, Laura Calderón Jiménez, Elena Sánchez Rodríguez, Miguel Recuero Vázquez, Helena Alonso Samperiz, Irene León Saiz, Juana Marcos Guerra

“…The study suggests that the Barthel Index may have limitations in adequately discriminating mortality risk.…”
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Mitigation of polycyclic aromatic hydrocarbons (PAHs) in roasted beef patties by cold plasma treatment and products quality evaluation by Yuke Hou, Yangjian Hu, Min Li, Jiahui Nong, Fengyuan Xie, Yuhan Fan, Jianhao Zhang, Xianming Zeng, Minyi Han, Xinglian Xu, Xia Wang

“…ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids, which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased. Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls, utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality. …”
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A qualitative study of stigma among disabled elders with stroke and their primary caregivers (脑卒中失能老人及其主要照顾者病耻感的质性研究) by KE Ke (柯珂), HE Juanfeng (贺娟凤), WANG Wei (汪维)

“…Results By analyzing and sorting out the data, 3 themes and 9 sub-themes related to the experience of stigma were put forward: cognitive attribution (label recognition, abnormal behavior, attitudes of others, lack of social support), negative emotional response (guilt and remorse, anomaly discrimination, belief in suicide) and non-adaptive coping style (social withdrawal, conceal illness). …”
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Effects of macitentan and selexipag across prognostic age groups in patients with pulmonary arterial hypertension by Richard Channick, MD, Sarah Medrek, MD, Marion Delcroix, MD, Sean Gaine, MD, Pavel Jansa, MD, PhD, Irene Lang, MD, Vallerie McLaughlin, MD, Sanjay Mehta, MD, Tomas Pulido, MD, Bhagavatula Sastry, MD, Rogerio Souza, MD, PhD, Adam Torbicki, MD, Carol Zhao, MS, Paul Strachan, MD, Peter Agron, PhD, Joseph Yen, PhD, Olivier Sitbon, MD, PhD

“…RESULTS: Three age groups (< 35, 35–64, ≥ 65 years) showed good M/M risk discrimination (c-statistic 0.69, SERAPHIN; 0.66, GRIPHON). …”
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Clinical determinants and prognostic implications of renin and aldosterone in patients with symptomatic heart failure by Masatake Kobayashi, Susan Stienen, Jozine M. terMaaten, Kenneth Dickstein, Nilesh J. Samani, Chim C. Lang, Leong L. Ng, Stefan D. Anker, Macro Metra, Gregoire Preud'homme, Kevin Duarte, Zohra Lamiral, Nicolas Girerd, Patrick Rossignol, Dirk J. vanVeldhuisen, Adriaan A. Voors, Faiez Zannad, João Pedro Ferreira

“…By reflecting both the disease and its treatments, the prognostic discrimination of these biomarkers was poor. Our data suggest that the “point” measurement of renin and aldosterone in HF is of limited clinical utility.…”
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Influencing factors of IgA nephropathy combined with hyperuricemia and a nomogram to predict the risk of hyperuricemia in IgA nephropathy patients by Zhou Jie, Xian Wei-ying, Li Ji-tian, Zeng Jin-cheng

“…The receiver operating characteristic （ROC） curve was plotted to evaluate the discriminability of the nomogram in predicting HUA in IgAN patients. …”
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Exploring the Interaction between Local Communities and Environmental Policies: A Qualitative Study of Conflicts between Natives and Rangers around the Protected Areas of Bojaq Nat... by Yaser Khoshsirat Salimi, Ali Ruhani, Hossein Afrasiabi, Sadegh Salehi

“…Discussion of Results & Conclusion The findings revealed 140 subthemes and 7 main themes, which included: employment challenges, feelings of discrimination, inheritance and disinheritance, internal solidarity and resistance, protection and management issues, and effective management practices. …”
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Research on formant estimation algorithm for high order optimal LPC root value screening by Hua LONG, Shumeng SU

“…So the amplitude of speech frequency is corrected, and the energy matching between the spectral peak of the speech signal and the spectral peak of LPC is enhanced, the distance between poles is extended, the prediction error caused by harmonic generation interference is reduced, and the peak frequency discrimination of spectrum is improved. Results: As can be seen from the algorithm structure, the speech signal is preprocessed, in which the low frequency information is reweighted to reduce the interference of fundamental frequency to formant detection.And the high frequency information is enhanced to increase the amplitude distinction of the third formant in the high spectrum line. …”
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Construction of a C-reactive protein-albumin-lymphocyte index-based prediction model for all-cause mortality in patients on maintenance hemodialysis by Junmin Huang, Junfeng Hao, Huasheng Luo, Lu Chen, Hongying Luo, Huafeng Liu, Yongzhi Xu, Peng Wang

“…Multivariate logistic regression analysis was used to screen factors associated with death, and nomograms were developed to estimate the risk of death in MHD patients. The discrimination and calibration of nomograms were validated using the area under the receiver operating characteristic (ROC) curve (AUC) and calibration curve. …”
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Prognostic Value of Skull Base Foramen Invasion Subclassification in T Category Modification and Induction Chemotherapy Management for Nasopharyngeal Carcinoma: Post‐Hoc Analysis o... by Siyu Zhu, Shuqi Li, Di Cao, Chao Luo, Zhiying Liang, Shaobo Liang, Guoyi Zhang, Qin Zhao, Guangying Ruan, Lizhi Liu, Gui Fu, Haojiang Li

“…Reclassifying T3 with severe SBFI as the T4 category yields an improved T category discrimination. Additionally, patients in the severe SBFI group gain significant survival benefits from induction chemotherapy ((IC). …”
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Effect of Dam Emplacement and Water Level Changes on Sublacustrine Geomorphology and Recent Sedimentation in Jackson Lake, Grand Teton National Park (Wyoming, United States) by Michael M. McGlue, John R. Dilworth, Hillary L. Johnson, Samuel J. Whitehead, J. Ryan Thigpen, Kevin M. Yeager, Edward W. Woolery, Summer J. Brown, Sarah E. Johnson, Cooper S. Cearley, Gillian M. Clark, T. Spencer Dixon, Ryan C. Goldsby, Autumn L. Helfrich, Bailee N. Hodelka, Edward L. Lo, Leandro Domingos-Luz, Nicholas E. Powell, Giliane G. Rasbold, William R. Swanger

“…Because the Teton fault is a major seismic hazard, end-member characterization of turbidites is a critical first step for accurate discrimination of mass transport deposits controlled by earthquakes in more ancient Jackson Lake strata. …”
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Association of thoracic aortic calcium with incident cardiovascular disease and all-cause mortality across the spectrum of coronary artery calcium burden by Alexander C. Razavi, Omar Dzaye, Miguel Cainzos-Achirica, Zeina Dardari, Marly Van Assen, Arshed A. Quyyumi, Khurram Nasir, J. Jeffrey Carr, Matthew J. Budoff, Roger S. Blumenthal, Paolo Raggi, Carlo N. De Cecco, Laurence S. Sperling, Michael J. Blaha, Seamus P. Whelton

“…The addition of TAC to traditional risk factors and CAC did not improve CVD discrimination (ΔC-statistic=+0.002, p=0.12), but incrementally improved prediction of all-cause mortality (CVD: ΔC-statistic=+0.002, p=0.02). …”
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Associations between local COVID-19 policies and anxiety in the USA: a longitudinal digital cohort study by Jeffrey E Olgin, Gregory M Marcus, Rita Hamad, John Kornak, Carmen R Isasi, Ana Sanchez-Birkhead, Mark Pletcher, Soo Park, Natasha Williams, Thomas Carton, Aaron E Cozen, Madelaine Faulkner Modrow, Amy Chiang, Matthew Brandner, Jaime H Orozco, Kristen Azar, Sylvia E K Sudat, Pelin Ozluk, Heather Kitzman, Sara J Knight

“…More comprehensive containment policies were associated with elevated anxiety among people working in strongly affected sectors, and more comprehensive public health policies were associated with elevated anxiety among people vulnerable to racial discrimination.…”
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