Metabolomic profiling and antibacterial efficacy of probiotic-derived cell-free supernatant encapsulated in nanostructured lipid carriers against canine multidrug-resistant bacteri... by Nay Zin Myo, Nay Zin Myo, Ratchnida Kamwa, Ratchnida Kamwa, Thitirat Jamnong, Busaba Swasdipisal, Papavarin Somrak, Phanchompoo Rattanamalakorn, Vipada Neatsawang, Prasert Apiwatsiri, Teerapong Yata, David J. Hampson, Nuvee Prapasarakul, Nuvee Prapasarakul

Get full text

Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction by Muktiningsih Nurjayadi, Gladys Indira Putri, Jefferson Lynford Declan, Ismaya Krisdawati, Dandy Akbar Juliansyah, Maharanianska Azzahra, Irvan Maulana, Irma Ratna Kartika, Fera Kurniadewi, Tiara Fahriza, Adinda Myra Amalia Putri, Ayu Berkahingrum, Atikah Nur Rahmawati, Rosita Gio Anggraeni, Dalia Sukmawati, Sri Rahayu, Vira Saamia, I Made Wiranatha, Bassam Abomoelak, Hesham Ali El-Enshasy

Get full text

Targeted Next-Generation Sequencing in Pneumonia: Applications in the Detection of Responsible Pathogens, Antimicrobial Resistance, and Virulence by Liu B, Bao Z, Chen W, Xi X, Ge X, Zhou J, Zheng X, Zhang P, Deng W, Ding R, Zhou M, Fang J

“…Bing Liu,1– 3,* Zhiyao Bao,1– 3,* Wei Chen,1– 3,* Xiaotong Xi,4,5,* Xiao Ge,1– 3 Jun Zhou,1– 3 Xiaoyan Zheng,6 Peipei Zhang,4,5 Wanglong Deng,4,5 Ran Ding,4,5 Min Zhou,1– 3 Jie Fang7 1Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People’s Republic of China; 2Institute of Respiratory Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People’s Republic of China; 3Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases, Shanghai, 200025, People’s Republic of China; 4State Key Laboratory of Neurology and Oncology Drug Development, Jiangsu Simcere Diagnostics Co., Ltd., Nanjing Simcere Medical Laboratory Science Co., Ltd., Nanjing, 210018, People’s Republic of China; 5Nanjing Simcere Medical Laboratory Science Co., Ltd., Nanjing, 210018, People’s Republic of China; 6Department of Pulmonary and Critical Care Medicine, Zhoushan Branch of Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Zhoushan, 316000, People’s Republic of China; 7Department of Pharmacy, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jie Fang, Department of Pharmacy, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Rui Jin 2nd Road, Shanghai, 200025, People’s Republic of China, Email fj40517@rjh.com.cn Min Zhou, Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197, Rui Jin 2nd Road, Shanghai, 200025, People’s Republic of China, Email doctor_zhou_99@163.comBackground: Targeted next-generation sequencing (tNGS) is a high-throughput and cost-effective diagnostic alternative for pneumonia, with the ability to simultaneously detect pathogens, antimicrobial resistance genes, and virulence genes. …”
Get full text

Unveiling GPT-4V's hidden challenges behind high accuracy on USMLE questions: Observational Study by Zhichao Yang, Zonghai Yao, Mahbuba Tasmin, Parth Vashisht, Won Seok Jang, Feiyun Ouyang, Beining Wang, David McManus, Dan Berlowitz, Hong Yu

“…MethodsThis cross-sectional study tested GPT-4V, GPT-4, and ChatGPT-3.5 Turbo on a total of 227 multiple-choice questions with images from USMLE Step 1 (n=19), Step 2 clinical knowledge (n=14), Step 3 (n=18), the Diagnostic Radiology Qualifying Core Exam (DRQCE) (n=26), and AMBOSS question banks (n=150). …”
Get full text

Small Intestinal Bacterial Overgrowth in Various Specialties of Medical Practice (Literature Review and Expert Council Resolution) by V. T. Ivashkin, V. V. Fomin, O. N. Tkacheva, O. S. Medvedev, E. A. Poluektova, D. I. Abdulganieva, S. R. Abdulkhakov, O. P. Alexeeva, S. A. Alekseenko, D. N. Andreev, A. Yu. Baranovsky, M. S. Zharkova, O. Yu. Zolnikova, K. V. Ivashkin, I. L. Kliaritskaia, N. V. Korochanskaya, S. N. Mammaev, R. V. Maslennikov, R. G. Myazin, M. V. Perekalina, A. V. Povtoreyko, A. I. Ulyanin, M. V. Fadeeva, I. B. Khlynov, V. V. Tsukanov, O. S. Shifrin

“…Aim: to discuss current views on the clinical significance, diagnostic opportunities, and therapeutic approaches in the treatment of small intestinal bacterial overgrowth (SIBO) as an important component in the gut microbiota function assessment, to assess the awareness of physicians and the opportunities in the diagnosis and treatment of this disease in patients in the Federal districts of the Russian Federation, as well as to present the proceedings of the Expert Council held on December 16, 2023 in Moscow.Key points. …”
Get full text

Hidden Urban Biodiversity: A New Species of the Genus <i>Scincella</i> Mittleman, 1950 (Squamata: Scincidae) from Chengdu, Sichuan Province, Southwest China by Ru-Wan Jia, Zong-Yuan Gao, Di-Hao Wu, Guan-Qi Wang, Gang Liu, Min Liu, Ke Jiang, De-Chun Jiang, Jin-Long Ren, Jia-Tang Li

“…This discovery underscores the underestimated diversity of <i>Scincella</i> in China and highlights the importance of urban habitats as reservoirs of hidden biodiversity. A diagnostic key to the <i>Scincella</i> species of China is also provided.…”
Get full text

Sources and Health Risks of PM2.5-bound PAHs in a Small City along with the “Clean Heating” Policy by Zhiyong Li, Ziyuan Yue, Jixiang Liu, Zhen Zhai, Jianwei Meng, Lei Wang, Jihong Wei, Dingyuan Yang, Ailian Hu, Wenjia Zhu, Ning Ding, Xiaoxia Lu, Chunhao Lin

“…The far lower PAHs in this study than those of small cities before CH implementation indicated the CH effectiveness. Higher diagnostic ratios (DRs) of FA/(FA + PY), BaA/(BaA + CHR), and IP/(IP + BgP) in the HS were in agreement with the increased coal/biomass usage. …”
Get full text

Subclinical Plasmodium spp. Infections in a Community Setting in Bangui, Central African Republic by Nzoumbou-Boko R, Denissio Morissi Nalingbo MCI, Yambiyo BM, Detol R, Moussa ERF, Nalinga D, Namsenei-Dankpea LJVDLG, Manirakiza A, Ayong L, Boum II Y

“…Proportions of positive tests for malaria parasites were determined by combining the results of antigen-based malaria rapid diagnostic test (mRDT), thick blood smear microscopy, and polymerase chain reaction (PCR).Results: A total of 638 participants (mean age, 26.44 years (range, [1– 75] years) with a sex ratio (M/F) of 1.22) were tested for malaria using all three detection methods. …”
Get full text

ATP1B3 may promote glioma proliferation and migration through MAPK/NF-KB signaling pathway by Qikang Yan, Quan Sun, Yan Feng, Qingyun Hu, Jinling Zhu

“…As a result, the ATP1B3 gene might be a biological target for treatment and a possible neurotumor diagnostic.…”
Get full text

Reduced Serum Glucagon Like Peptide-1 In Children with Osteoporosis of Chronic Liver Diseases: A Single Center Trial by Nashwa F. Mohamed Elmetwaly, Ola G. Behairy, Manal S. EL-Defrawy, Ola E. Seleim, Dina S. Abdelmotaleb

“…Low serum 25(OH) vit D is a sensitive and specific diagnostic marker of osteoporosis in CLD.…”
Get full text

Neovascular glaucoma as a complication of proton therapy for uveal melanoma by I.E. Panova, E.M. Svistunova, N.A. Vorobyov, E.V. Samkovich, N.I. Martynova, A.A. Bykhovsky

“…Petersburg,&nbsp;&nbsp; Russian Federation </p> <p> ⁴Medical and Diagnostic Center of the International Institute of Biological Systems&nbsp;&nbsp; named after Berezin Sergei, St. …”
Get full text

Safety and efficacy of telaprevir in treatment of a chronic hepatitis C in patients of the Russian population included in early access program study by D. T. Abrurakhmanov, V. G. Morozov, I. G. Nikitin, P. O. Bogomolov, N. I. Geyvandova, Ye. N. Bessonova, I. G. Bakulin, V. A. Isakov, K. V. Zhdanov, M. V. Mayevskaya, Ye. A. Nurmukhametova, E. Z. Burnevich, Ye. V. Chesnokov, V. D. Pasechnikov, A. Hill, I. Lonjon-Domanec, S. N. Kizhlo

Get full text

Integrative single-cell and bulk RNA-seq analyses identify CD4+ T-cell subpopulation infiltration and biomarkers of regulatory T cells involved in mediating the progression of athe... by Yifeng Zhang, Yifeng Zhang, Shuxian Lu, Shuxian Lu, Liang Qiu, Liang Qiu, Manman Qin, Manman Qin, Dan Shan, Lianhua Xie, Yao Yi, Yao Yi, Jun Yu

“…We also employed high dimensional weighted gene co-expression network analysis (hdWGCNA) and cell-cell communication analysis to elucidate the modules and cascade reaction of Tregs in the progression of AS. The key genes diagnostic potential was assessed via receiver operating characteristic (ROC) curve analysis. …”
Get full text

Indocyanine Green Fluorescence Plus Blue Dye for Sentinel Lymph Node Biopsy in Patients Undergoing Neoadjuvant Chemotherapy for Breast Cancer: A Multicenter, Prospective Cohort Stu... by Miao Liu, Yang Yang, Bin Hua, Rui Feng, Tianyu Xu, Mengyuan Wang, Xiaowei Qi, Yingming Cao, Bo Zhou, Fuzhong Tong, Peng Liu, Hongjun Liu, Lin Cheng, Houpu Yang, Fei Xie, Siyuan Wang, Chaobin Wang, Yuan Peng, Danhua Shen, Lei Chen, Jun Jiang, Shu Wang

“…This study aims to examine diagnostic performance of dual indocyanine green (ICG) and methylene blue tracing for SLNB in patients who have completed NAC for breast cancer with initially cN1 disease. …”
Get full text

Specific immune response to M. tuberculosis and ability to in vitro control mycobacterial replication are not impaired in subjects with immune-mediated inflammatory disease and tub... by Chiara Farroni, Anna Maria Gerarda Altera, Andrea Salmi, Valentina Vanini, Valentina Vanini, Gilda Cuzzi, Cecilia S. Lindestam Arlehamn, Alessandro Sette, Giovanni Delogu, Giovanni Delogu, Ivana Palucci, Settimia Sbarra, Alessandra Aiello, Andrea Picchianti-Diamanti, Gina Gualano, Fabrizio Palmieri, Delia Goletti, Elisa Petruccioli

Get full text

Application of lacrimal gland ultrasonography in the evaluation of chronic ocular graft-versus-host-disease by Mingxia Zhong, Siyuan Liu, Jinghan Luo, Qin Zhang, Zhou Yang, Shanshan Zhang

“…Patients were categorized into groups according to the International Chronic oGVHD (ICCGVHD) consensus group diagnostic criteria or the 2005 National Institutes of Health (NIH) classification criteria for Chronic GVHD. …”
Get full text

The Relationship Between Serum miRNAs and Surgical Prognostic Factors in Gastric Cancers by Çağrı Büyükkasap, Engin Küçükdiler, Mahir Nasirov, Niyazi Samet Yılmaz, Hasan Dağlı, İlke Önen, Furkan Aydoğdu, Aydın Yavuz, Hüseyin Göbüt, Asiye Uğraş Dikmen, Kürşat Dikmen, Hasan Bostancı, Özlem Gülbahar, Abdulkadir Bedirli

“…Conclusion: Serum levels of miR-17-5p, miR-21-5p, miR-27a-3p, and miR-203a-3p were found to be diagnostic biomarkers in gastric cancers. The expression levels of serum miR-106b-5p and miR-146a-5p were found to be related to tumor location, whereas the expression levels of serum miR-27a-3p and miR-148a-5p were found to be related to the T-stage of the tumor. …”
Get full text

Evidence Gaps and Lessons in the Early Detection of Atrial Fibrillation: A Prospective Study in a Primary Care Setting (PREFATE Study) by Josep L. Clua-Espuny, Alba Hernández-Pinilla, Delicia Gentille-Lorente, Eulàlia Muria-Subirats, Teresa Forcadell-Arenas, Cinta de Diego-Cabanes, Domingo Ribas-Seguí, Anna Diaz-Vilarasau, Cristina Molins-Rojas, Meritxell Palleja-Millan, Eva M. Satué-Gracia, Francisco Martín-Luján

“…<b>Conclusions:</b> The study underscores the feasibility and utility of AF screening in primary care but identifies critical gaps in diagnostic criteria, anticoagulation thresholds, and guideline recommendations.…”
Get full text

Disrupting USP39 deubiquitinase function impairs the survival and migration of multiple myeloma cells through ZEB1 degradation by Jessy Sirera, Saharnaz Sarlak, Manon Teisseire, Alexandrine Carminati, Victoria J. Nicolini, Coline Savy, Patrick Brest, Thierry Juel, Christophe Bontoux, Marcel Deckert, Mickael Ohanna, Sandy Giuliano, Maeva Dufies, Gilles Pages, Frederic Luciano

“…Conclusions Our findings highlight the critical role of the deubiquitinase USP39, suggesting that the USP39/ZEB1 axis could serve as a potential diagnostic marker and therapeutic target in MM.…”
Get full text

The role of mNGS in the diagnosis of talaromycosis and case series by Jing Zhao, Hui-Ting Su, Dong Zhang, Xue-Tong Li, Min-Ya Lu, Juan Du, Jia-Yu Guo, Yi Gao, Xin-Fei Chen, Dong-Hua Wen, Shi-Yu Jia, Yao Wang, Qi-Wen Yang

“…Conclusions Traditional diagnostic methods are limited by factors such as the long time and low sensitivity of fungal culture, the effect of prior antibiotic treatment, the invasiveness and complexities of tissue biopsies, and so on. …”
Get full text