Performance of the AmpliSeq NGS panel in thyroid nodules with indeterminate cytology by Wiame Potonnier, Erell Guillerm, Claude Bigorgne, Cécile Ghander, Malanie Roy, Florence Coulet, François Ansart, Fabrice Menegaux, Laurence Leenhardt, Isabelle Brocheriou, Gabrielle Deniziaut, Camille Buffet

“…Conclusions: The performances of the AmpliSeq panel are promising; however, the NPV is not sufficient to avoid diagnostic surgery in cytologically indeterminate thyroid nodules.…”
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Optical genome mapping: Unraveling complex variations and enabling precise diagnosis in dystrophinopathy by Jiahui Mai, Jing Duan, Xiaoyu Chen, Liqin Liu, Dachao Liang, Tao Fu, Gang Lu, Wai Yee Chan, Xufeng Luo, Feiqiu Wen, Jianxiang Liao, Zhuo Li, Xinguo Lu

“…Interpretation OGM is a valuable diagnostic tool for dystrophinopathy patients with negative results from conventional genetic tests. …”
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New pneumococcal serotype 20C is a WciG O-acetyltransferase deficient variant of canonical serotype 20B by Jigui Yu, Neil Ravenscroft, Peter Davey, Roshan Liyanage, Oliver Lorenz, Michelle M. Kuttel, Stephanie W. Lo, Feroze A. Ganaie, Moon H. Nahm

“…Since the appearance of new capsule types can directly affect pneumococcal conjugate vaccine (PCV) implementation, a deeper understanding of capsule PS at the genetic, immunological, and biochemical levels is critical for the development of future diagnostic tools and vaccines.…”
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Evaluation of three novel antigens and costimulatory agents for improvement of M. Tuberculosis specific interferon gamma release assays by Sandra Schwarzlose-Schwarck, Mark Reinwald, Torsten Bauer, Florian Hentschel, Til Kiderlen, Dorinja Zapf, Victor Herbst, Stefan Lüth, David Krieger, Werner Dammermann

“…Furthermore, we specifically investigated the potential of the complement factor C5a and the toll like receptor (TLR) agonists CpG ODN as well as Poly(I: C) as costimulators in order to increase diagnostic quality of MT IGRAs. Three MT IGRAs were evaluated, i.e. our in-house IGRA, a prototypic EUROIMMUN Quan-T-Cell TB assay and the gold standard QuantiFERON Tb-Gold Plus assay. …”
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Myocardial Infarction-Associated Shock: A Comprehensive Analysis of Phenotypes, SCAI Classification, and Outcome Assessment by Stanislav Dil, Maria Kercheva, Oleg Panteleev, Sergey Demianov, Aleksandr Kanev, Nina Belich, Boris Kornienko, Vyacheslav Ryabov

“…MS is associated with elevated mortality rates and presents unique diagnostic and therapeutic challenges. This study aimed to analyze the clinical, historical, instrumental, and laboratory characteristics of the primary phenotypes of MI-CS, stratified according to the Society for Cardiovascular Angiography and Interventions (SCAI) shock severity scale. …”
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Study Protocol of the Korean EGFR Registry: A Multicenter Prospective and Retrospective Cohort Study in Nonsmall Cell Lung Cancer Patients With EGFR Mutation by Chang Dong Yeo, Dong Won Park, Seong Hoon Yoon, Eun Young Kim, Jeong Eun Lee, Shin Yup Lee, Chang‐Min Choi, In‐Jae Oh, Do Jin Kim, Jeong Seon Ryu, Jae Cheol Lee, Young‐Chul Kim, Tae Won Jang, Kye Young Lee, Seung Hun Jang, Seung Joon Kim, for the Korean EGFR Registry Investigators

“…The study will explore the diagnostic methods for EGFR mutations, clinical outcomes based on treatment modalities, and metastatic patterns in EGFR‐mutated NSCLC patients. …”
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Evaluation of the double-zone hemolysis (DZH) test for the detection of livestock-associated methicillin-resistant Staphylococcus aureus by Javier Latorre-Fernández, Carmen Aspiroz, Idris Nasir Abdullahi, Allelen Campaña-Burguet, Paula Eguizábal, Carmen González-Azcona, Carmen Tenorio, Myriam Zarazaga, Adebayo O. Shittu, Carmen Lozano, Carmen Torres

“…The DZH is a reliable strategy to detect and distinguish LA-MRSA in the clinical microbiology laboratory and is recommended as an adjunct diagnostic test.IMPORTANCEThis study evaluated a simple and reliable phenotypic test that can be very useful in the clinical microbiology laboratory to detect livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and S. aureus of potential animal origin. …”
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Genome-Wide Association Study of Bone Mineral Density in Korean Men by Ye Seul Bae, Sun-Wha Im, Mi So Kang, Jin Hee Kim, Soon Hang Lee, Be Long Cho, Jin Ho Park, You-Seon Nam, Ho-Young Son, San Deok Yang, Joohon Sung, Kwang Ho Oh, Jae Moon Yun, Jong Il Kim

“…Bone mineral density (BMD), a highly heritable trait, has been proven a useful diagnostic factor in predicting fracture. Because medical information is lacking about male osteoporotic genetics, we conducted a genome-wide association study of BMD in Korean men. …”
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Prevalence and factors associated with childhood malaria and anaemia in Osun state, Nigeria: a baseline household malariometric study by Olusola Ajibaye, Semiu Adebayo Rahman, Oluwagbemiga Olanrewaju Aina, Chinazo Ujuju, Chimere Obiora Agomo, Samuel Akindele, Olakiigbe Abiodun, Tolulope Victoria Uzoka, Nnenna Ogbulafor, Olufemi Oroge, Rufai-Ahmed Garba, Michael Ekholuenetale, Kolawole Maxwell, Ridwan Akorede Awosanya, Mary Abosede Adekola, Benjamin Bukky Ilesanmi, Adekemi Ajayi, Olusola Oresanya, James K. Tibenderana, Adeola Yetunde Olukosi

“…Malaria test positivity was determined by rapid diagnostic test (RDT) and microscopy. In addition, haemoglobin levels were measured using Haemocue® Hb 201. …”
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Phenotypic and genotypic characterization of Mycobacterium tuberculosis pyrazinamide resistance—India, 2018–2020 by Sembulingam Tamilzhalagan, Evanslin Santus Justin, Ashok Selvaraj, Karthick Venkateswaran, Arun Kumar Sivakumar, Suganthi Chittibabu, Heather P. McLaughlin, Patrick K. Moonan, Jonathan P. Smith, Sakthi Suba, Mukesh Kumar Sathya Narayanan, Christine S. Ho, Nishant Kumar, Srikanth P. Tripathy, Srikanth P. Tripathy, Siva K. Shanmugam, Patricia J. Hall-Eidson, Uma Devi Ranganathan

“…This nationwide study was conducted to explore the prevalence of mutations conferring PZA resistance, catalogue mutation diversity, investigate the associations of PZA resistance with specific lineages, examine co-resistance to 13 first- and second-line drugs, and evaluate the diagnostic accuracy of sequencing pncA and panD genes for predicting PZA resistance. …”
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Micro- and ultramorphological features of the leaf cells of Myrtus communis L. as a parameter for the standardization of medicinal plant syrup are the basis for new herbal remedies by V. M. Odyntsova, O. Ye. Matsehorova, S. I. Tertyshnyi, Yu. F. Polkovnikov

“…The aim of the work is to study the morphological and anatomical structure and determine the general diagnostic microscopic features of leaves and the structure of meristem cells of common myrtle. …”
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Dual‐Enhanced SERS Satellite Immuno‐Nanocomplex for Multiple PSA‐Mediated PHI Assay Toward Clinical Prostate Cancer Screening by Dong Chen, Yilin Ma, Annan Yang, Liping Hu, Hanlin Zhou, Jun Xu, Shanze Chen, Dingmeng Nie, Weifeng Feng, Huaihong Cai, Yanguang Cong, Jiang Pi, Lang Rao, Xueqin Huang, Pinghua Sun, Haibo Zhou

“…Unlike the previous studies focus on the tPSA and fPSA/tPSA ratio (f/t PSA%), this work introduces a multiple PSA‐mediated Prostate Health Index (PHI) assay with significantly increased the predictive accuracy and specificity of PCa, especially the patients with a tPSA level in the “diagnostic gray zone”. Practical analysis of clinical samples demonstrated the SERS immunoassay is capable of identifying the PCa subjects from healthy candidates (N = 13), and monitoring PCa patients before and after surgery (N = 5), as well as screening suspicious ones who are in the “gray zone” (N = 10). …”
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Towards a histological diagnosis of childhood small vessel CNS vasculitis by Maryam Nabavi Nouri, Anastasia Dropol, Pascal N. Tyrrell, Sheila Sheikh, Marinka Twilt, Jean Michaud, Benjamin Ellezam, Harvey B. Sarnat, Christopher Dunham, Peter W. Schutz, Julia Keith, David G. Munoz, Harry V. Vinters, Cynthia Hawkins, Susanne M. Benseler

“…The presence of leptomeningeal inflammation in isolation was non-diagnostic. Conclusion Distinct histological features were identified in sv-cPACNS biopsies, including moderate to severe angiocentric inflammatory infiltrates in the cortex or white matter, consisting of CD3, CD4, CD8, and CD20 cells, alongside reactive endothelium with specificity of 95%. …”
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Intraspecific variation and new morphological characters revealed by multimodal imaging analysis on the Late Cretaceous coleoid Dorateuthis syriaca by Alison J. Rowe, Isabelle Kruta, Loïc Villier, Pierre Gueriau, Marie Radepont, Oulfa Belhadj, Katharina Müller, Romain Jattiot, Dirk Fuchs, Thomas Clements, Sylvain Charbonnier, Isabelle Rouget

“…Despite being the most abundant coleoid from these localities, the taxon lacks clear, unambiguous diagnostic characteristics of both the gladius, and soft tissue anatomy. …”
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New Record of <i>Encarsia protransvena</i> and Confirmed Occurrence of <i>Encarsia hispida</i> (Hymenoptera: Aphelinidae) as Parasitoids of <i>Singhiella simplex</i> (Hemiptera: Al... by Giuliano Cerasa, Luigi Tomasello, Gianluca Melone, Elia Russo, Gaetano Siscaro, Carmelo Cavallaro, Annamaria Ienco, Francesca Laudani, Vincenzo Palmeri, Orlando Campolo, Francesca Garganese, Francesco Porcelli, Paolo A. Pedata, Vittorio Farina, Giovanni Gugliuzza, Roberto Rizzo, Stefania Laudonia, Gabriella Lo Verde

“…Comprehensive illustrations and diagnostic features are provided for the host and the parasitoids. …”
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Comparative abundance and diversity of populations of the Pseudomonas syringae and Soft Rot Pectobacteriaceae species complexes throughout the Durance River catchment from its Fren... by Morris, C.E., Lacroix, C., Chandeysson, C., Guilbaud, C., Monteil, C., Piry, S., Rochelle Newall, E., Fiorini, S., Van Gijsegem, F., Barny, M.A., Berge, O.

“…Our observations suggest that surveillance of river water for SRP could be leveraged to signal diagnostic and management reactions to avoid disease outbreaks.  …”
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Seroprevalence of Malaria and Hepatitis B Coinfection among Pregnant Women in Tamale Metropolis of Ghana: A Cross-Sectional Study by Gideon Kofi Helegbe, Paul Armah Aryee, Baba Sulemana Mohammed, Anthony Wemakor, David Kolbila, Abdul-Wahid Abubakari, Salam Askanda, Rashid Alhassan, Collins Barnie, Afua Aboagyewaa Donkoh, Ernest Ofosu

“…Methods. By means of rapid diagnostic tests (RDTs), pregnant women attending the Tamale Teaching Hospital (TTH) were screened for malaria, HBV infection, HIV infection, and syphilis from March 2013 to February 2015. …”
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Volume contracted state, mortality and functional outcomes in patients with acute ischaemic stroke due to large vessel occlusion by Risheng Xu, Max Wintermark, Gregory W Albers, Jeremy J Heit, Adam A Dmytriw, Adrien Guenego, Yasmin Aziz, Kambiz Nael, Benjamin Pulli, Victor Urrutia, Dhairya A Lakhani, Tobias D Faizy, Argye Hillis, Vivek Yedavalli, Dylan Wolman, Licia P Luna, Elisabeth B Marsh, Hamza Adel Salim, Janet Mei, Vaibhav Vagal, Aakanksha Sriwastwa, Raf Llinas, Hanzhang Lu, Mona N Bahouth

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Development of a Predictive Model of Occult Cancer After a Venous Thromboembolism Event Using Machine Learning: The CLOVER Study by Anabel Franco-Moreno, Elena Madroñal-Cerezo, Cristina Lucía de Ancos-Aracil, Ana Isabel Farfán-Sedano, Nuria Muñoz-Rivas, José Bascuñana Morejón-Girón, José Manuel Ruiz-Giardín, Federico Álvarez-Rodríguez, Jesús Prada-Alonso, Yvonne Gala-García, Miguel Ángel Casado-Suela, Ana Bustamante-Fermosel, Nuria Alfaro-Fernández, Juan Torres-Macho

“…<i>Conclusions</i>: This is the first risk score developed for identifying patients with VTE who are at increased risk of occult cancer using ML tools, obtaining a remarkably high diagnostic accuracy. This study’s limitations include potential information bias from electronic health records and a small cancer sample size. …”
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Isomeric Differentiation of Four Triterpene Acids Using Full Collision Energy Ramp-MS2 Analysis by Li-juan WU, Rui-ru NAN, Qian WANG, Xin CHEN, Wen-jing LIU, Yue-lin SONG

“…The four isomers were firstly divided into two groups, designated as group I (ursolic acid and oleanolic acid) and group II (α-boswellic acid and β-boswellic acid) through the existences of the so-called diagnostic fragment ions. Moreover, the two isomers belonging to group I show different OCE and RIImax values for m/z 411 and 439, respectively. …”
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