Experience on postmortem minimally invasive tissue sampling to ascertain the cause of death determination in South African children: A case for implementing as standard of care by J du Toit, K Storath, I Dunn, P Makekeng, M Moosa, K Mothibi, N Umuneza, C A Rees, D Blau, S Lala, Y Adam, S Velaphi, M Hale, P Swart, J Wadula, L Mothibi, A Wise, V Baba, P Jaglal, S Mahtab, S Madhi, Z Dangor

“…MITS proves a valid alternative to full diagnostic autopsies, especially in low- and middle-income countries. …”
Get full text

Identification and validation of a novel autoantibody biomarker panel for differential diagnosis of pancreatic ductal adenocarcinoma by Metoboroghene O. Mowoe, Metoboroghene O. Mowoe, Hisham Allam, Joshua Nqada, Marc Bernon, Karan Gandhi, Sean Burmeister, Urda Kotze, Miriam Kahn, Christo Kloppers, Suba Dharshanan, Zafirah Azween, Pamela Maimela, Paul Townsend, Eduard Jonas, Jonathan M. Blackburn, Jonathan M. Blackburn

“…Subsequently, the specificity of the 11-biomarker panel was validated against other cancers (PDAC vs PC: AUC = 70·3%; PDAC vs CRC: AUC = 84·3%; PDAC vs PRC: AUC = 80·2%) and healthy controls (PDAC vs HC: AUC = 80·9%), confirming that this novel AAb biomarker panel is able to selectively detect PDAC amongst other confounding diseases.ConclusionThis AAb panel may therefore have the potential to form the basis of a novel diagnostic test for PDAC.…”
Get full text

STR profiling in a cohort of Saudi patients with acute leukemia by Husein A. Alhatim, PhD Student, Muhammad NH Abdullah, PhD, Suhaili A. Jamaludin, PhD, Armania B. Nurdin, PhD, Sayed A. Amer, PhD

“…Further study applying next generation sequencing technology is necessary to validate these findings and explore the clinical applications of forensically used STRs as diagnostic tools for leukemia.…”
Get full text

Pragmatic algorithm for visual assessment of 4-Repeat tauopathies in [18F]PI-2620 PET Scans by Theresa Bauer, Matthias Brendel, Mirlind Zaganjori, Alexander M. Bernhardt, Alexander Jäck, Sophia Stöcklein, Maximilian Scheifele, Johannes Levin, Thilo van Eimeren, Alexander Drzezga, Osama Sabri, Henryk Barthel, Robert Perneczky, Günter Höglinger, Nicolai Franzmeier, Johannes Gnörich

“…Combined assessment of multiple brain regions did not significantly improve diagnostic sensitivity, compared to assessing the basal ganglia alone (76% each). …”
Get full text

Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis... by Insaf Bel Hadj Ali, Yusr Saadi-Ben Aoun, Imen Khammeri, Hejer Souguir, Emna Harigua-Souiai, Hamed Chouaieb, Ahmed S. Chakroun, Meryem Lemrani, Aicha Kallel, Kalthoum Kallel, Nabil Haddad, Oussaima El Dbouni, Rhea N. Coler, Rhea N. Coler, Rhea N. Coler, Steven G. Reed, Akila Fathallah-Mili, Akila Fathallah-Mili, Ikram Guizani

“…Here, we aimed for a simple and rapid molecular diagnostic test to detect and identify L. major, a predominant CL causal species, which could be prone to become a control tool at the point of care, in endemic areas, using isothermal recombinase DNA amplification (recombinase polymerase amplification, RPA, or recombinase aided amplification, RAA) coupled to detection by the lateral flow (LF) chromatography on a PCRD cassette.MethodsTo develop an L. major species-specific RPA-LF assay, computational analysis of 70 Leishmania DNA targets, identified through bibliography and database searches, selected five targets. …”
Get full text

Blood Pressure Variability during Angiography in Patients with Ischemic Stroke and Intracranial Artery Stenosis by Hui Pan, Rong Zhao, Feng-Di Liu, Yi-Lan Wu, Ge-Fei Li, Yan-Hui Shi, Yi-Sheng Liu, Ying Zhao, Mei-Ting Zhuang, Tian-Yu Hou, Qi-Ting Zhang, Qian Yao, Yuan Qiao, Rui-Jue Huang, Li-Na Chen, Yi-Min Zhu, Liang Shu, Jing-Jing Su, Jie Fang, Xue-Mei Tang, Shuo Wang, Guo-Hong Cui, David Z. Wang, Jian-Ren Liu

“…Our aim was to investigate factors predicting blood pressure (BP) variability during diagnostic cerebral angiography and associations between BP variability and clinical outcomes in patients with acute and subacute ischemic stroke and intracranial artery stenosis. 114 patients with ischemic stroke and intracranial artery stenosis (stenosis rate >50%) were recruited. …”
Get full text

Clonal hematopoiesis in patients with autoimmune thrombocytopenia: an international multicenter study by Bruno Fattizzo, Alfredo Marchetti, Alessandro Bosi, Carmelo Gurnari, Juri Alessandro Giannotta, Giacinto Luca Pedone, Elena Rossi, Valentina Carrai, Andrea Guido, Filippo Brioschi, Monica Carpenedo, Monica Crugnola, Domenica Caramazza, Livia Leuzzi, Monia Marchetti, Gabriele Merati, Simona Malato, Fabrizio Vianello, Andrea Patriarca, Hussein Awada, Marta Bortolotti, Marta Canzi, Niccolò Bolli, Marco Capecchi, Frederick Chen, Andrea Artoni, Jaroslaw P. Maciejewski, Wilma Barcellini

“…Abstract: Diagnostic boundaries between immune thrombocytopenia (ITP) and other thrombocytopenic states, such as thrombocytopenic myelodysplastic syndromes, may be difficult to establish, and the detection of somatic mutations by next-generation sequencing (NGS) may be of aid. …”
Get full text

Détection de différents rétrovirus chez des primates non-humains infectés par STLV-1 ou SFV et pathologie viro-induite by Jocelyn Turpin, Sandrine Alais, Ambroise Marçais, Olivier Hermine, Réjane Rua, Antoine Gessain, Romain Lacoste, Renaud Mahieux

“…Des lésions de la peau évocatrices de celles retrouvées chez les patients ATL étaient observées. Le diagnostic d'ATL lymphomateux fut posé. Un traitement expérimental associant Combivir (AZT) et viraferon (interféron-alpha) et qui est utilisé chez les patients ATL chronique ou aiguë fut proposé. …”
Get full text

Labeling tumor-associated extracellular vesicles with antibody-DNA conjugates for quantitative analysis by Xiao Du, Xiao Du, Xiao Du, Hongxiu Li, Hongxiu Li, Hongxiu Li, Shiyi Shen, Shiyi Shen, Shiyi Shen, Chao Tian, Chao Tian, Chao Tian, Xiaohuan Cao, Xiaohuan Cao, Xiaohuan Cao, Xingang Xu, Nan Xu, Shuling Wang, Shuling Wang, Shuling Wang, Qingchang Tian, Qingchang Tian, Qingchang Tian

“…Antibody-DNA conjugates were used to recognize the epithelial cell adhesion molecule (EpCAM) antigen on the TAE surface and quantify the antigen using qPCR for cancer analysis.DiscussionThis method proposed a new quantitative detection approach for TAEs, which aim to identify specific EV-associated markers for diagnostic or therapeutic, this method could inspire a new idea for tumor diagnosis and detection of other diseases.…”
Get full text

Congenital T-cell activation impairs transitional-to-follicular B-cell maturation in humans by Hugues Allard-Chamard, Kirsty Hillier, Michelle L. Ramseier, Alice Bertocchi, Naoki Kaneko, Katherine Premo, Grace Yuen, Marshall Karpel, Vinay S. Mahajan, Christina Tsekeri, Joseph S. Hong, Jean Vencic, Rory Crotty, Anish V. Sharda, Sara Barmettler, Emma Westermann-Clark, Jolan E. Walter, Musie Ghebremichael, Alex K. Shalek, Jocelyn R. Farmer, Shiv Pillai

Get full text

Differentiation of high risk prostate cancer with a facile urinary exosome detection workflow by Shuai Qiu, Yue Li, Zheng Zhang, Chunchang Li, Haoyu Wang, Ao Chen, Zhi Yan, Yang Liu, Zifei Li, Hua Huang, Yi Liu, Yiqi Seow, Ruibing Chen, Jinhong Guo, Simeng Wen, Jing Tian, Hongtuan Zhang, Ranlu Liu, Gang Han, Baolong Wang, Yong Wang, Yuanjie Niu, HaiFang Yin

“…Here, we developed a proprietary urinary exosome isolation approach (EVLatch) and established a facile diagnostic workflow. We discovered that EEF1A1 levels, abundantly expressed on urinary exosomes, positively correlate to urinary exosome counts irrespective of source and collection time and demonstrated that EEF1A1 enables in-assay quantification of urinary exosomes. …”
Get full text

Psychological discomfort in carriers and non-carriers of the Huntington disease mutation and its relationship with disease burden by Y. Rodríguez-Agudelo, M. Chávez-Oliveros, A. Ochoa-Morales, L. Martínez-Ruano, A. Camacho-Molina, F. Paz-Rodríguez

“…Method: A sample of 175 participants in a HD Predictive Diagnostic Program (PDP-HD) was divided into HEP carriers (39.4%) and NPEH non-carriers (61.6%) of the HD-causing mutation. …”
Get full text

SECTM1 acts as an immune-related biomarker of poor prognosis and promotes cancer progression by modulating M2 macrophage polarization in esophageal squamous cell carcinoma by Pengzhou Kong, Pengzhou Kong, Pengzhou Kong, Ye Jiao, Ye Jiao, Meng Sun, Meng Sun, Zhinan Zhou, Zhinan Zhou, Yingying Zhang, Yingying Zhang, Xin Yang, Xin Yang, Jing Ren, Jing Ren, Mengyuan Yang, Mengyuan Yang, Yanyan Dong, Yanyan Dong, Yanyan Dong, Bin Song

“…Esophageal squamous cell carcinoma (ESCC) is the most prevalent primary malignant esophageal tumor in China and has a poor prognosis, but lacks effective diagnostic and prognostic biomarkers. Through single-sample gene set enrichment analysis (ssGSEA), we conducted immune genomic analysis based on 28 immune features using transcriptomic data from 155 ESCC cases. …”
Get full text

The effect of the number of endometrial CD138+ cells on the pregnancy outcomes of infertile patients in the proliferative phase by Yuye Li, Yuye Li, Shuyi Yu, Shuyi Yu, Wenjuan Liu, Yawen Chen, Xiaobing Yang, Juanhua Wu, Mingjuan Xu, Guanying You, Ruochun Lian, Chunyu Huang, Chunyu Huang, Wanru Chen, Yong Zeng, Yong Zeng, Fenghua Liu, Lianghui Diao

“…The analysis demonstrated that the smallest number of stromal CD138+ cells suggestive of CE patients requiring treatment was defined as ≥ 3 positive lesions during the proliferation.ConclusionsDifferent diagnostic criteria for CE should be created for the proliferative and mid-luteal phases. …”
Get full text

Multi-omics analysis reveals the interplay between intratumoral bacteria and glioma by Ting Li, Zhanyi Zhao, Meichang Peng, Lu Zhang, Cheng Wang, Feiyang Luo, Meiqin Zeng, Kaijian Sun, Zhencheng Fang, Yunhao Luo, Yugu Xie, Cui Lv, Jiaxuan Wang, Jian-Dong Huang, Hongwei Zhou, Haitao Sun

“…Through in vivo and in vitro experiments, we identified the crucial role of Fn in promoting glioma progression, suggesting that Fn could be a potential diagnostic and therapeutic target for glioma patients. …”
Get full text

Quantifying the spatial patterns of retinal ganglion cell loss and progression in optic neuropathy by applying a deep learning variational autoencoder approach to optical coherence... by Jui-Kai Wang, Jui-Kai Wang, Brett A. Johnson, Zhi Chen, Zhi Chen, Honghai Zhang, Honghai Zhang, David Szanto, Brian Woods, Brian Woods, Michael Wall, Young H. Kwon, Young H. Kwon, Edward F. Linton, Edward F. Linton, Andrew Pouw, Mark J. Kupersmith, Mark J. Kupersmith, Mark J. Kupersmith, Mona K. Garvin, Mona K. Garvin, Mona K. Garvin, Mona K. Garvin, Randy H. Kardon, Randy H. Kardon

“…Future work will focus on integrating additional image modalities to further refine the model’s diagnostic capabilities.…”
Get full text

Protocol for Home-Based Solution for Remote Atrial Fibrillation Screening to Prevent Recurrence Stroke (HUA-TUO AF Trial): a randomised controlled trial by Jing Tan, Yan Liang, Yong Liu, Wei Jiang, Duo Huang, Ning Tan, Chung-Wah Siu, Jo Jo Hai, Xiaoyu Huang, Jianfeng Ye, Ji-yan Chen, Chun Ka Wong, Mi Zhou, Li-Xue Yin, Ming-Liang Zuo, Kui Kai Lau, Zhen Lei, Yingqing Feng, Bryan P Yan, Wen-Sheng Yue, Wen-Jun Zhang, Yuk-Ming Lau, Hin-Wai LUI, Koon-Ho Chan, Toi Meng Mok, Weng-Chio Tam, Kun-Chong Tam, Xiuhua Feng, Xiaofei Jiang, Guanming Tan, Mario Alberto Evora

“…All remotely obtained data will be automatically transmitted in real-time through the study smartphone application to a secured cloud hosting and analysed using an artificial intelligence-based diagnostic system. When a diagnosis of AF is made with the system, the patients will be called back for a formal cardiology consultation within 1 week. …”
Get full text

Correlations between primary tumour location, biomarkers of inflammation and lung injury, and postoperative pulmonary complications in patients underwent laparoscopic colorectomy:... by Hui-xian Li, Li Che, Yuan Li, Tai-hang Wang, Fang-di Min, Lei Xu, Meng Wang, Zhao-xu Zheng, Shi-ning Qu, Fei Wang, Wei Tang, Shi-jing Wei, Yu-lin Sun, Hui Zheng, Tao Yan

Get full text

Application of cellular microstructural diffusion MRI (cell size imaging) in rectal lesions: a preliminary study by Peisi Kou, Liangjie Lin, Ying Li, Hui Qin, Kun Zhang, Wenhua Zhang, Juan Li, Yong Zhang, Jingliang Cheng

“…The receiver operating characteristic (ROC) curve analysis was used to evaluate diagnostic power. The sensitivity, specificity, and the corresponding area under the curves (AUCs) were calculated.ResultsOur preliminary results illustrated that malignant lesion showed higher vin and cellularity ([0.2867 ± 0.0697] vs. [0.1856 ± 0.1011], [2.3508 ± 0.6055] vs. [1.2716 ± 0.4574], all P<0.05), lower dex and ADC values (ADCPGSE, ADC17Hz, and ADC of conventional DWI) compared to benign lesion ([2.1637 ± 0.3303 μm2/ms] vs. [2.5595 ± 0.5085 μm2/ms], [0.9238 (0.7959, 1.0741) ×10-3 mm2/s] vs. [1.3373 ± 0.3902×10-3 mm2/s], [1.3204 ± 0.2342×10-3 mm2/s] vs. [1.8029 ± 0.3119×10-3 mm2/s], [0.7400 (0.6750, 0.8375) ×10-3 mm2/s] vs. [1.0550 ± 1.1191×10-3 mm2/s], all P<0.05), while no significant difference was seen for dmean. …”
Get full text

Health systems analysis and evaluation of the barriers to availability, utilisation and readiness of sexual and reproductive health services in COVID-19-affected areas: a WHO mixed... by José Guilherme Cecatti, Kun Tang, Igor Toskin, Moazzam Ali, Caron Kim, Stefano Uccella, Simone Garzon, Ge Yang, Vanessa Brizuela, Pisake Lumbiganon, Seni Kouanda, Alexandra Sawyer, Nigel Sherriff, Alessia Savoldi, Anna Thorson, Marleen Temmerman, Jörg Huber, Soe Soe Thwin, Maddalena Cordioli, Massimo Mirandola, Jaime Vera, Armando Seuc, Laila Ladak, Debbie Williams, Luis Bahamondes, Deda Ogum Alangea, Abdu Mohiddin, Eunice Nahyuha Chomi, Sothornwit Jen, Modey Emefa, Hamsadvani Kuganantham, Armando Humberto Seuc, Eunice Chomi, Hanxiyue Zhang, Yifan Zhu, Yueping Guo, Chunxiao Peng, Kwasi Tropsey, Emefa Judith Modey, Rozina Karmaliani, Jen Sothornwit, Ferdinand Okwaro, Ranieri Poli, Joy Jerop Chebet, Hugo Gamerro Abrego

Get full text