Expression of PD-1 on CD4+ Tumor-Infiltrating Lymphocytes in Tumor Microenvironment Associated with Pathological Characteristics of Breast Cancer by Yan-Jie Zhao, Jian Zhang, Feng Shi, Zhi-Ping Hu, Jiang-Ping Wu, Guang-Jiang Wu, Rui-Bin Wang, Quan Zhou, Hong Chang, Ying-Nan Li, Qing-Kun Song

“…The expression of CD4, programmed cell death protein 1 (PD-1), CK7, CK20, E-cadherin, or Ki-67 was detected by immunohistochemistry. The associations between CD4+/PD-1+ or CD4+/PD-1− tumor-infiltrating lymphocytes and pathological characteristics were evaluated. …”
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Time-resolved Hubble Space Telescope UV Observations of an X-Ray Quasiperiodic Eruption Source by T. Wevers, M. Guolo, S. Lockwood, A. Mummery, D. R. Pasham, R. Arcodia

“…Here, we report on time-resolved, coordinated Hubble Space Telescope far-ultraviolet (FUV) and XMM-Newton X-ray observations of the shortest period X-ray QPE source currently known, eRO-QPE2. We detect a bright UV point source ( L _FUV ≈ few × 10 ^41 erg s ^−1 ) that does not show statistically significant variability between the X-ray eruption and quiescent phases. …”
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Unleashing the potential of a low CpG Passer transposon for superior CAR-T cell therapy by Jianyao Zeng, Yan Sun, Yan Sun, Yuan Fang, Xiaodie Wang, Qian Huang, Pingjing Zhang, Meiqi Shao, Pei Wang, Jingbo Cheng, Meng Di, Tao Liu, Qijun Qian, Qijun Qian, Qijun Qian

“…BALB/c mice and M-NSG mice were used to evaluate the inflammatory response and efficacy of LCG CAR-T in vivo, with TIL grouping detected via immunohistochemistry.ResultsIn this study, we modified the newly discovered Passer (JL) transposon to construct a low-CpG content transposon for CAR-T cell (LCG CAR-T cell) preparation. …”
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Pleiotropic Effects of a KCNQ1 Variant on Lipid Profiles and Type 2 Diabetes: A Family-Based Study in China by Xiaowen Wang, Junhui Wu, Yao Wu, Mengying Wang, Zijing Wang, Tao Wu, Dafang Chen, Xun Tang, Xueying Qin, Yiqun Wu, Yonghua Hu

“…Furthermore, the common genetic predisposition to T2DM and lipids was not fully detected. Methods. In total, 5839 individuals (2220 were T2DM patients) across 2885 families were included. …”
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Reverse-Phase Chromatographic Determination and Intrinsic Stability Behavior of 5-[(4-Chlorophenoxy)Methyl]-1,3,4-Oxadiazole-2-Thiol by Shehzadi Naureen, Hussain Khalid, Islam Muhammad, Bukhari Nadeem Irfan, Khan Muhammad Tanveer, Salman Muhammad, Siddiqui Sabahat Zahra, Rehman Aziz-Ur, Abbasi Muhammad Athar

“…Isocratic elution was carried out at a flow rate of 1.0 mL min-1 through a Promosil C18 column maintained at 25 °C, using the mobile phase comprising acetonitrile and aqueous o-H3PO4 (pH 2.67) (1:1, V/V). Detection was performed at 258 nm. The response of the detector was linear in a concentration range of 1.25-50.00 μg mL-1 with the correlation coefficient of 0.9996 ± 0.0001. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…The concentrations of IFN-γ and TNF-α were calculated using ELISA kits, and the proteins were detected using western blot. Results. In CD11c+ DCs derived from the spleens with hyperglycemia, the expression of CD83 and CD86 in diabetic mice was significantly upregulated, coupled with a higher secretion level of cytokines and chemokines, and increased phosphorylation of NF-κB and IκB. …”
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The important roles of ERAP1, ERAP2 genes polymorphisms and their DNA methylation levels in pulmonary tuberculosis by Shi-Hong Su, Xue-Qian Cai, Yong-Huai Li, Ai-Hui Xu, Qian Huang, Hua Niu, Qing-Hai You, Geng-Yun Sun

“…Methods We genotyped ten single nucleotide polymorphisms (SNPs) in ERAP1, ERAP2 genes among 497 PTB patients and 502 controls by SNPscan technique. Additionally, we detected the ERAP1, ERAP2 genes methylation levels in 98 PTB patients and 97 controls through Illumina Hiseq platform. …”
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Harnessing genomic and bioinformatic data to broaden understanding of leukaemia across continents by Gumelar Gugun, Ulfa Mia Maria, Amukti Danang Prasetyaning, Irham Lalu Muhammad, Yuliani Sapto, Adikusuma Wirawan, Khairi Sabiah, Darmawi Darmawi, Chong Rockie, Ates Ilker, Singh Dilpreet, Chavan Aditya Ashok

“…The data was then screened using SNPnexus to detect potentially protein-damaging variants. Furthermore, the gene expression of these variants was analysed using the GTEx portal. …”
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Spatial-temporal distribution of phytoplankton HAB species and contamination status of oyster toxins under intensive oyster farming in Jiangmen coasts, the South China Sea by Jian Zou, Yayuan Xiao, Jiaqi Su, Yang Liu, Peng Wu, Teng Wang, Lin Lin, Chunhou Li, Yu Liu, Yong Liu

“…Only spring oyster samples were detected paralytic shellfish toxins (PSTs) (1/15), and the composition included gonyautoxins (GTX1&2) and decarbamoyl gonyautoxin 2 (dcGTX2), with a total toxicity level of 9.96 µg STXeq kg−1. …”
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DKK3 promotes renal fibrosis by increasing MFF-mediated mitochondrial dysfunction in Wnt/β-catenin pathway-dependent manner by Jianling Song, Yanxia Chen, Yan Chen, Minzi Qiu, Wenliu Xiang, Ben Ke, Xiangdong Fang

“…Western blotting, IHC, and qRT-PCR were performed to detect molecular expression levels. Immunofluorescence was applied to determine the subcellular localization. …”
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An Enhanced Three-Band Algorithm for Retrieving Phycocyanin Concentration from Satellite Data in Plateau Inland Lake by Dajun Yuan, Bo-Hui Tang, Dong Wang, Junyi Chen, Zhen Zhang, Zhitao Fu

“…Phycocyanin (PC), a characteristic pigment of cyanobacteria, plays a crucial role in detecting cyanobacterial blooms and providing early warnings. …”
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Evaluation of potential productivity in coniferous forests by integrating field data and aerial laser scanning in Hidalgo, México by Rodrigo Ramos-Madrigal, Héctor M. de los Santos-Posadas, José René Valdez-Lazalde, Efraín Velasco-Bautista, Gregorio Ángeles-Pérez, Alma Delia Ortiz-Reyes

“… Aim of study: To predict the productivity potential of a managed conifer forest by estimating the site index from Light Detection and Ranging (LiDAR) data. Study area: Intensive Carbon Monitoring Site Atopixco, Hidalgo, Mexico. …”
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Noninvasive prenatal diagnosis (NIPD) of non-syndromic hearing loss (NSHL) for singleton and twin pregnancies in the first trimester by Huanyun Li, Shaojun Li, Zhenhua Zhao, Lingrong Kong, Xinyu Fu, Jingqi Zhu, Jun Feng, Weiqin Tang, Di Wu, Xiangdong Kong

“…Moreover, it provides a novel and advanced diagnostic approach for families at high risk of NSHL during pregnancy, offering earlier detection, enhanced safety, and improved accuracy.…”
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CHROMOGRANIN-A IN THE DIAGNOSIS OF PROSTATE CANCER by A. V. Sivkov, N. G. Keshishev, G. D. Efremov, G. A. Kovchenko, A. A. Trudov, L. M. Nikonova, F. D. Romikh

“…HGA is a high interest for the early detection CRPC. Also with the help of HGA it seem to be quite promising to discover the most aggressive forms of prostate cancer. …”
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Inflammatory rheumatic diseases with onset after SARS-CoV-2 infection or COVID-19 vaccination: a report of 267 cases from the COVID-19 and ASD group by Laura Massaro, Annamaria Iagnocco, Florenzo Iannone, Corrado Campochiaro, Maria De Santis, Ilaria Cavazzana, Piero Ruscitti, Roberto Giacomelli, Rosario Foti, Lorenzo Dagna, Giovanna Cuomo, Giacomo De Luca, Francesco Caso, Ilenia Di Cola, Clodoveo Ferri, Vincenzo Raimondo, Francesco Ursini, Veronica Brusi, Giuseppe Varcasia, Roberta Pellegrini, Domenico Olivo, Giuseppe Murdaca, Carlo Selmi, Olga Addimanda, Erika Pigatto, Francesca Francioso, Rossella De Angelis, Jacopo Ciaffi, Luana Mancarella, Marcella Visentini, Francesca Motta, Virginia Caira, Alberto Lo Gullo, Caterina Naclerio, Elena Marchetti, Sebastiano Lorusso, Jessica Luppino, Roberta Foti, Massimo Reta

“…No differences were detected in the percentage of patients diagnosed with connective tissue diseases (CTD 19.7% vs 20.7%, p=0.837) or vasculitis (6.6% vs 9.0%, p=0.467). …”
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Comprehensive Hematological and molecular Characterization of hemoglobin Hekinan [α27(B8)Glu→Asp(α1), HBA1:c.84G > T] in a Large Thai cohort by Amornchai Suksusut, Jidapa Jaitheang, Manussavee Prapphal, Pranee Sutcharitchan, Ponlapat Rojnuckarin, Noppacharn Uaprasert

“…Additional genetic determinants, including α-thalassemia, β-thalassemia and other variants, were detected using multiplex GAP-PCR, ARMS-PCR or direct DNA sequencing as appropriate.Results: Among 61,997 Hb typing samples, 149 cases of Hb Hekinan were identified in Thai individuals and classified into 8 genotypic groups. …”
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Subclinical patterns of cardiac involvement by transthoracic echocardiography in individuals with mild initial COVID-19 by Anastasia Shchendrygina, Mame Madjiguène Ka, Carlos Rodriguez, Safaa Alsoufi, Jedrzej Hoffmann, Parveen Kumar, Maria Ludovica Carerj, Byambasuren Vanchin, Niels Holm, Argyro Karyou, Mijidsuren Ganbat, Eike Nagel, Valentina O. Puntmann

“…All the measured differences were small in magnitude and thus unlikely to be detectable clinically at the individual level.…”
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Periodontitis promotes tumor growth and immune evasion via PD-1/PD-L1 by Suli Wang, Fujiao Nie, Qiuyue Yin, Haoyang Tian, Pizhang Gong, Jinhong Ju, Jiayi Liu, Pishan Yang, Chengzhe Yang

“…Tumor volume and weight were recorded and the levels of relevant immune-suppressive cells and T cells were detected by flow cytometry or immunofluorescence. THP-1 cells were stimulated using conditioned media of LPS-stimulated Cal-27 cells and PD-L1 expression was measured by quantitative real-time PCR, western blotting and immunofluorescence. …”
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Therapeutic target genes and regulatory networks of gallic acid in cervical cancer by Zhixi You, Ye Lei, Yongkang Yang, Zhihui Zhou, Xu Chao, Keyi Ju, Songyi Wang, Yuanyuan Li, Yuanyuan Li

“…The upstream 13 miRNAs, 4 lncRNA, and 42 circRNAs were detected and used to construct a lncRNA/circRNA-miRNA-mRNA-pathway regulatory network.ConclusionThis study identified candidate genes and the regulatory networks underlying the therapeutic effects of GA on CC using GA data mining methods, thus establishing a theoretical basis for targeted therapy of CC.…”
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New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments by Dejan Stojković, Nina Dragičević, Marija Ivanov, Nevena Gajović, Milena Jurišević, Ivan Jovanović, Marina Tomović, Jelena Živković

“…Results: The presence of numerous flavonoid and phenolic compounds such as sulfuretin, gallic acid, rutin, hyperoside, and isoquercitrine was detected. <i>Micrococcus luteus</i>, <i>Streptococcus parasanguinis</i>, and <i>Candida tropicalis</i> were the most sensitive microbiological species, with MICs of 0.12 mg/mL for the most effective extracts. …”
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