<i>Staphylococcus aureus</i> Proteins Implicated in the Reduced Virulence of <i>sarA</i> and <i>sarA/agr</i> Mutants in Osteomyelitis by Karen E. Beenken, Mara J. Campbell, Stephanie D. Byrum, Rick D. Edmondson, Samuel G. Mackintosh, Alan J. Tackett, Mark S. Smeltzer

“…A total of 1039 proteins were detected in conditioned media (CM) from overnight cultures of LAC, and protease-mediated degradation was shown to contribute to the reduced abundance of 224 of these (21.6%) in CM from the <i>sarA</i> and <i>sarA/agr</i> mutants. …”
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Runs of homozygosity in spontaneous abortions from families with recurrent pregnancy loss by N. A. Skryabin, S. A. Vasilyev, T. V. Nikitina, D. I. Zhigalina, R. R. Savchenko, N. P. Babushkina, M. E. Lopatkina, A. A. Kashevarova, I. N. Lebedev

“…The high frequency of extended ROHs detected in the present study may point to a role of inbreeding in RPL etiology. …”
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Antioxidant and Laxative Effects of Methanol Extracts of Green Pine Cones (<i>Pinus densiflora</i>) in Sprague-Dawley Rats with Loperamide-Induced Constipation by Hee-Jin Song, Ayun Seol, Jumin Park, Ji-Eun Kim, Tae-Ryeol Kim, Ki-Ho Park, Eun-Seo Park, Su-Jeong Lim, Su-Ha Wang, Ji-Eun Sung, Youngwoo Choi, Heeseob Lee, Dae-Youn Hwang

“…Furthermore, significant improvements in the stool parameters, gastrointestinal (GI) transit, intestine length, and histopathological structure of the mid colon were detected in the Lop-induced constipation rats after MPC treatment. …”
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Computerised cognitive training tools and online nutritional group counselling for people with mild cognitive impairment: study protocol of a completely digital, randomised, contro... by Stephanie Book, Elmar Graessel, Julia Schneider, Michael Jeitler, Petra Scheerbaum, Michael Jank, Etienne Hanslian, Melanie DellO’ro, Julia-Sophia Scheuermann, Sophia Bösl, Christian Kessler

“…With N=200, effects with an effect size of f≥0.24 (comparable to Cohen’s d≥0.48) can be detected. Screening, baseline, t6 and t12 testing will be conducted via a videoconferencing assessment, telephone, and online survey. …”
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Vesicoureteral Reflux in Children with Accidental Diagnosis of Unilateral Small Size Kidney by Mohsen Mohammadi, Khadijeh Ebrahimi, Soraya Khafri, Maryam Nikpour, Hadi Sorkhi

“…All children were followed for at least for 1 year with urine culture and urinalysis every 1-2 months for detection of UTI. Significance was set at P less than .05. …”
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Seropositivity to tick-borne pathogens in nature management workers in the Netherlands by B.J.A. Hoeve-Bakker, G. Çelik, O.E. van den Berg, C.C. van den Wijngaard, A. Hofhuis, J.H.J. Reimerink, S.F.T. Thijsen, K. Kerkhof

“…For B. burgdorferi s.l. antibody detection, the recommended two-tier testing strategy was used. …”
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Photometric Determination of Unresolved Main-sequence Binaries in the Pleiades: Binary Fraction and Mass-ratio Distribution by Rongrong Liu, Zhengyi Shao, Lu Li

“…This approach enables an accurate photometric determination of f _b and q distribution in a cluster. The detectable mass ratio can be down to the ${q}_{{\rm{lim}}}$ , limited by the minimum stellar mass in theoretical models. …”
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Strategies for H-score Normalization of Preanalytical Technical Variables with Potential Utility to Immunohistochemical-Based Biomarker Quantitation in Therapeutic Reponse Diagnost... by William E. Pierceall, Michele Wolfe, Jessica Suschak, Hua Chang, Yan Chen, Kam M. Sprott, Jeffery L. Kutok, Stella Quan, David T. Weaver, Brian E. Ward

“…Most interestingly, detectable expression of SNRPA and SnRNP70 is covariant to test biomarkers under distinct fixation conditions and so these hold the potential for serving as calibration standards for general antigen preservation and reactivity.…”
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Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells by Erwei Hao, Jianfeng Qin, Wei Wei, Jianhua Miao, Yan Xie, Xianglong Pan, Hangxuan Wu, Jinling Xie, Xiaosu Fan, Zhengcai Du, Xiaotao Hou, Jiagang Deng

“…By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG. Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester. …”
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Quality identification of Amomi fructus using E-nose, HS-GC-IMS, and intelligent data fusion methods by Pan-Pan Zhang, Xin-Jing Gui, Xin-Jing Gui, Xin-Jing Gui, Xue-Hua Fan, Han-Li, Hai-Yang Li, Xiao-Peng Li, Feng-Yu Dong, Yan-Li Wang, Jing-Yao, Jing-Yao, Jun-Han Shi, Jun-Han Shi, Rui-Xin Liu, Rui-Xin Liu, Rui-Xin Liu, Rui-Xin Liu, Rui-Xin Liu

“…A total of 111 VOCs were detected by HS-GC-IMS, with 101 tentatively identified. …”
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Pollen contamination and mating structure in maritime pine (Pinus pinaster Ait.) clonal seed orchards revealed by SNP markers by Bouffier, Laurent, Debille, Sandrine, Alazard, Pierre, Raffin, Annie, Pastuszka, Patrick, Trontin, Jean-François

“…All parental genotypes from the seed orchards contributed to the offspring as pollen parents, but differences in paternal reproductive success were detected. Finally, the overall self-fertilization rate was estimated at 5.4%, with considerable variability between genotypes (from 0% to 26%). …”
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The interplay between RNA m6A modification and radiation biology of cancerous and non-cancerous tissues: a narrative review by Yajia Cheng, Yue Shang, Shuqin Zhang, Saijun Fan

“…It begins with an overview of m6A modification and its detection methods, followed by a detailed examination of how m6A dynamically regulates the sensitivity of cancerous tissues to RT, the injury response in non-cancerous tissues, and the toxicological effects of UV exposure. …”
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Ethanol Extracts of Solanum lyratum Thunb Regulate Ovarian Cancer Cell Proliferation, Apoptosis, and Epithelial-to-Mesenchymal Transition (EMT) via the ROS-Mediated p53 Pathway by Chen Zhang, Zheming Li, Jie Wang, Xuelu Jiang, Mengting Xia, Jianfen Wang, Shenyi Lu, Shouye Li, Hanmei Wang

“…Furthermore, A2780 and SKOV3 cells cocultured with M0 macrophages showed that SLT activated the polarization of M0 macrophages to M1 macrophages and inhibited the polarization to M2 macrophages, with the increased percentage of CD86+ cells and decreased percentage of CD206+ cells were detected. In summary, this study illustrated the anticancer effects of SLT on ovarian cancer cells, suggesting that SLT may have the potential to provide basic evidence for the discovery of antiovarian cancer agents.…”
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Abdominal fat distribution and its relationship to brain changes: the differential effects of age on cerebellar structure and function: a cross-sectional, exploratory study by Yvonne Böttcher, Michael Stumvoll, Kees Straatman, Matthias Raschpichler, Matthias Leopold Schroeter, Katrin Arelin, Haiko Schlögl, Dominik Fritzsch, Meinhard Mende, André Pampel, Arno Villringer, Karsten Mueller

“…Investigating functional brain architecture showed again inverse VAT–cerebellum correlations, whereas now regions involved in cognitive and emotional processing were significant. Although we detected similar results for EC using BMI, significant age interaction for both brain structure and functional architecture was only found using VAT.Conclusions Visceral adiposity is associated with cerebellar changes of both structure and function, whereas the regions involved contribute to motor, cognitive and emotional processes. …”
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Exploring the effects of Tianma Gouteng granules on L-NAME-induced hypertensive rats based on 16S rDNA gene sequencing and metabolomics by Li Cheng, Zhenyang Huang, Jiawei He, Xinyi Zhang, Jiangxue Di, Hanmei Jiang, Yi Liu

“…After drug administration, 16S rDNA gene sequencing and non-targeted metabolomics were applied to detect and analyze the intestinal flora and fecal metabolites of the rats in each group. …”
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Standardised protocol for a prospective cross-sectional multicentre clinical utility evaluation of two dual point-of-care tests in non-clinical settings for the screening of HIV an... by James Kiarie, Karel Blondeel, Igor Toskin, Jordi Casabona, Soe Soe Thwin, Massimo Mirandola, Rosanna Peeling, Ronald Ballard, Laura Fernàndez-López, Inga Upmace, Juliana Reyes-Urueña, Mara Vaselova, Mitja Ćosić, Simon Maljevac, William Mejías, Ander Pazos, Andrii Chernyshev

“…Introduction Point-of-care dual tests (POCTs) for simultaneously detecting antibodies to HIV and syphilis (dual HIV-syphilis POCTs) have been developed recently and show encouraging performance compared with the reference tests in laboratory-based studies. …”
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Interaction effect between vitamin D and gestational diabetes mellitus in the second trimester by MA Ziwen, DING Xiaoying, CHU Liming, ZHANG Yan, ZHANG Aifeng, ZHU Yanjun, WANG Xun, XIE Zhenyu

“…The level of serum 25(OH)D at the time of OGTT were detected and other clinical indicators were followed up.ResultsThe age, systolic blood pressure in early pregnancy, pre-pregnancy BMI, FPG, OGTT 1-hour and 2-hour glucose, GHb, HOMA-IR, TG, AST, Cr, D-D, FDP and SF at 35 weeks’ gestation of the pregnant women were higher in the GDM group than that of the pregnant women in the normal group, while gestational weight gain and serum 25(OH)D level were significantly lower than that of the pregnant women in the normal group. …”
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Microsatellite analyses reveal genetic diversity and population structure of Cipangopaludina chinensis in Guangxi, China by Xiao-kai Wei, Kang-qi Zhou, Xin-xi Zou, Xue-ling Zhang, Yu-Sen Li, Hui Luo, Yin Huang, Xue-song Du, Jun-qi Qin, Zhong Chen, Yong Lin, Xian-hui Pan, Hua Ye

“…The results showed that a total of 176 alleles in the populations were detected by 12 microsatellite markers. The polymorphism information content (PIC) was 0.523–0.918, indicating that these markers were highly polymorphic (PIC > 0.5). …”
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Evaluating the Accessibility of Urban Public Open Spaces Based on an Improved 2SFCA Model: A Case Study Within Chengdu’s Second Ring Road by Ling Jian, Xiaojiang Xia, Yinbing Zhao, Yang Zhang, Yuanqiao Wang, Yi Tang, Jie Chang, Changliu Wang

“…The accessibility results are further analyzed using hotspot analysis, and blind zone detection. In terms of supply improvements, the model incorporates additional indicators beyond the spatial area of UPOS, including service quality and the diversity of surrounding environmental service functions, to better evaluate the overall attractiveness of UPOS to residents. …”
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Identification of Potential Biomarkers and Immune Features of Sepsis Using Bioinformatics Analysis by Fang-Chen Gong, Ran Ji, Yu-Ming Wang, Zhi-Tao Yang, Ying Chen, En-Qiang Mao, Er-Zhen Chen

“…Markers currently in use have shortcomings such as a lack of specificity and failures in the early detection of sepsis. In this study, we aimed to identify key genes involved in the molecular mechanisms of sepsis and search for potential new biomarkers and treatment targets for sepsis using bioinformatics analyses. …”
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