Recombinant AAV batch profiling by nanopore sequencing elucidates product-related DNA impurities and vector genome length distribution by Florian Dunker-Seidler, Kathrin Breunig, Magdalena Haubner, Florian Sonntag, Markus Hörer, Rebecca C. Feiner

“…We contrasted recent nanopore sequencing advancements such as the V14 chemistry and dorado basecalling software with the widespread V9 chemistry and detected a markedly increased read quality. Our data highlight a high vector batch quality with low plasmid-derived and host cell DNA impurities of random origin, critical for mitigating associated safety risks. …”
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Blinder Oaxaca and Wilk Neutrosophic Fuzzy Set-Based IoT Sensor Communication for Remote Healthcare Analysis by Osamah Ibrahim Khalaf, Rajesh Natarajan, Natesh Mahadev, Prasanna Ranjith Christodoss, Thangarasu Nainan, Carlos Andres Tavera Romero, Ghaida Muttashar Abdulsahib

“…The result is conducted by using PYTHON application to analyze stress detection with the WESAD dataset. The proposed BO-SWNF method achieved an overall accurate data analysis of 12 % with minimum time ensuring 56 % improvement and minimizing energy consumption by 54 %.…”
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A Multicenter Observational Prospective Cohort Study of Association of the Prehospital National Early Warning Score 2 and Hospital Triage with Early Mortality by Francisco Martín-Rodríguez, Raúl López-Izquierdo, Carlos del Pozo Vegas, Juan F. Delgado-Benito, Carmen del Pozo Pérez, Virginia Carbajosa Rodríguez, Agustín Mayo Iscar, José Luis Martín-Conty, Carlos Escudero Cuadrillero, Miguel A. Castro-Villamor

“…The Prehospital Emergency Medical Services should evaluate the implementation of the NEWS2 as a routine evaluation, which, together with the structured hospital triage system, effectively serves to predict early mortality and detect high-risk patients.…”
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Adapting to evolving MRI data: A transfer learning approach for Alzheimer’s disease prediction by Rosanna Turrisi, Sarthak Pati, Giovanni Pioggia, Gennaro Tartarisco

“…Integrating 3D magnetic resonance imaging (MRI) with machine learning has shown promising results in healthcare, especially in detecting Alzheimer’s Disease (AD). However, changes in MRI technologies and acquisition protocols often yield limited data, leading to potential overfitting. …”
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Macrophage Migration Inhibitory Factor Interacting with Th17 Cells May Be Involved in the Pathogenesis of Autoimmune Damage in Hashimoto’s Thyroiditis by Haibo Xue, Yuhua Yang, Ying Zhang, Shoujun Song, Li Zhang, Lei Ma, Tingting Yang, Huan Liu

“…The percentages of Th17 cells and expressions of MIF, interleukin 17A (IL-17A) mRNA in PBMCs, as well as serum concentrations of MIF, and IL-17A, and thyroid functions, and thyroid-specific autoantibodies (TPOAb, TgAb) were detected by flow cytometry, real-time RT-PCR, ELISA, and ECLIA in all subjects. …”
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The Mechanisms of the Regulation of Immune Response in Patients with Comorbidity of Chronic Obstructive Pulmonary Disease and Asthma by Elena P. Kalinina, Yulia K. Denisenko, Tatyana I. Vitkina, Elena G. Lobanova, Tatyana P. Novgorodtseva, Marina V. Antonyuk, Tatyana A. Gvozdenko, Vera V. Knyshova, Anna V. Nazarenko

“…The elevated levels of TNF-α and IL-4 and inhibition of IFN-γ production were detected. The content of LTB4 was maximal; TXB2 levels were higher than in control group but lower than in COPD and asthma. …”
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Accurate prediction for electro-oxidation regeneration of chromium- containing waste acid based on artificial neural network by SHI Yaqi, MENG Guangyuan, CHEN Peng, ZHANG Xinwan, FU Tao, YANG Zhengwu, ZHANG Liansheng, ZHANG Lehua

“…Since it was difficult to achieve real-time detection of Cr(Ⅵ) content in this highly acidic system, a study based on artificial neural network was conducted to accurately predict the electro-oxidation regeneration effect of chromium-containing waste acid. …”
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Biallelic SLC13A1 loss-of-function variants result in impaired sulfate transport and skeletal phenotypes, including short stature, scoliosis, and skeletal dysplasia by Christina G. Tise, Katie Ashton, Lachlan de Hayr, Kun-Di Lee, Omkar L. Patkar, Emma Krzesinski, Jennifer A. Bassetti, Erin M. Carter, Cathleen Raggio, Andreas Zankl, Anas M. Khanshour, Kristhen N. Atala, Jonathan J. Rios, Carol A. Wise, Ying Zhu, Futao Zhang, Tony Roscioli, Michael Buckley, Robert J. Harvey, Paul A. Dawson

“…A reduction in plasma sulfate level and/or increase in urinary sulfate excretion was detected in 2 of 2 probands evaluated. Functional studies were consistent with SLC13A1 variants resulting in the complete loss of sulfate transport activity. …”
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Optimisation and validation of Plume Chasing for robust and automated NOx and particle vehicle emission measurements by Christina Schmidt, C. David Carslaw, J. Naomi Farren, N. René Gijlswijk, Markus Knoll, E. Norbert Ligterink, Jan Pieter Lollinga, Martin Pechout, Stefan Schmitt, Michal Vojtíšek, Quinn Vroom, Denis Pöhler

“…The methods were easy to automate and suitable for high emitter detection and quantification of emissions from two-wheeled vehicles. …”
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Accuracy and Usability of Smartphone-Based Distance Estimation Approaches for Visual Assistive Technology Development by Giles Hamilton-Fletcher, Mingxin Liu, Diwei Sheng, Chen Feng, Todd E. Hudson, John-Ross Rizzo, Kevin C. Chan

“…<italic>Methods:</italic> We tested five smartphone-based distance-estimation approaches in the image center and periphery at 1&#x2013;3 meters, including machine learning (CoreML), infrared grid distortion (IR_self), light detection and ranging (LiDAR_back), and augmented reality room-tracking on the front (ARKit_self) and back-facing cameras (ARKit_back). …”
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Emergence of Pituitary Adenoma in a Child during Surveillance: Clinical Challenges and the Family Members’ View in an AIP Mutation-Positive Family by Pedro Marques, Sayka Barry, Amy Ronaldson, Arla Ogilvie, Helen L. Storr, Peter J. Goadsby, Michael Powell, Mary N. Dang, Harvinder S. Chahal, Jane Evanson, Ajith V. Kumar, Joan Grieve, Márta Korbonits

“…Two years later, his brother was diagnosed with a somatolactotrophinoma, and a small Rathke’s cleft cyst and a microadenoma were detected on screening in their 17-year-old sister. …”
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Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress by Hao Zhang, Xiaobo Zhao, Quanxi Sun, Caixia Yan, Juan Wang, Cuiling Yuan, Chunjuan Li, Shihua Shan, Fengzhen Liu

“…A comprehensive analysis between two groups detected 3,425 differentially expressed genes (DEGs) including 2,013 upregulated genes and 1,412 downregulated genes. …”
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Effect of Narrow Band Ultraviolet B Therapy versus Methotrexate on Serum Levels of Interleukin-17 and Interleukin-23 in Egyptian Patients with Severe Psoriasis by Tarek Mahmoud Elghandour, Sahar El Sayed Youssef, Dalia Gamal Aly, Mohamed Said Abd Elhameed, Mehrevan Mostafa Abdel Moneim

“…A positive correlation was detected between the percent of reduction of IL-17, IL-23 and the percent of reduction of PASI score for patients receiving both treatments. …”
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Urine amino acid and gamma aminobutyric acid level in COVID 19 patients by Cemal Kazezoglu, Arzu İrvem, Yasemin Tuba Sutasir, Büşra Çakiroglu, Banu Kirgiz, Habip Yilmaz, Ali Kocatas

“…Elevated GABA in 28/30 cases, high hydroxylysine amino acid in 27/30 cases, low glycine in 30/30 cases were detected in the urine. The creatinine levels of the patients were found to be normal. …”
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Antibacterial activity of selenium nanoparticles/copper oxide (SeNPs/CuO) nanocomposite against some multi-drug resistant clinical pathogens by Ahmed Morad Asaad, Sara A. Saied, Mohammad M. Torayah, N. I. Abu-Elsaad, Samah Mohammed Awad.

“…The fabricated Se-NPs/Cu2O nanocomposites were detected as stable and highly crystallized nanospheres with an average size of 98.6 nm. …”
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Cooperation-Based Modeling of Sustainable Development: An Approach from Filippov’s Systems by Jorge A. Amador, Johan Manuel Redondo, Gerard Olivar‐Tost, Christian Erazo

“…For an isolated community and through a bifurcation analysis in which the Hopf bifurcation and the heteroclinic cycle bifurcation are detected, 4 prospective scenarios are found, but only one is sustainable under different extraction policies. …”
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HER2 expression and pathway status in male breast cancer patients: results of an integrated analysis among 6,150 patients by Boqiang Lyu, Shidi Zhao, Hui Wang, Shouping Gong, Biyuan Wang

“…Altered genes in HER2 + MBC, such as ERBB2, AGO2, RECQL4, and CLTC, were not detected in HER2-MBC. Genomic analysis revealed differences between the patients with HER2 + MBC and those with HER2 + FBC. …”
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Erectile Dysfunction Preceding Clinically Diagnosed α-Synucleinopathies: A Case-Control Study in Olmsted County by Shemonti Hasan, Michelle M. Mielke, J. Eric Ahlskog, James Bower, Pierpaolo Turcano, Rodolfo Savica

“…We reviewed complete medical records of cases and controls to detect erectile dysfunction prior to the clinical-motor onset of α-synucleinopathies of any type. …”
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Association of haplotypes for SNPs in the LTR regions of bovine leukemia virus with hematological indices of cattle by N. V. Blazhko, S. Kh. Vyshegurov, A. S. Donchenko, K. S. Shatokhin, T. I. Krytsyna, V. A. Ryabinina

“…Only viruses of haplotypes I (0.33±0.03) and III (0.67±0.03) of the eight possible were detected. The ratio of hematologically sick, healthy, and suspected carriers of BLV of haplotypes I and II was comparable with the results of other researchers. …”
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Leuko-glycemic Index as a Prognostic Factor after Acute Myocardial Infarction with ST-segment Elevation by Ailed Rodríguez Jiménez, Raikel Fardales Rodríguez, Enrique Toledo Rodríguez, Guillermo Quintana Cañizarez

“…<br /><strong>Conclusions:</strong> the leuko-glycemic index in the course of an acute myocardial infarction can be associated with a higher in-hospital mortality. Its detection would be useful in the prognostic stratification of acute coronary syndrome.…”
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