Liquid biopsy approaches to capture tumor evolution and clinical outcomes during cancer immunotherapy by Susan Scott, Mark Sausen, Valsamo Anagnostou, Jennifer Jackson, Vincent Lam, Joseph C Murray, Lavanya Sivapalan, Jenna Vanliere Canzoniero, Blair Landon, Benjamin P. Levy

“…Tracking the landscape of genomic alterations detected in ctDNA may reveal the clonal architecture of the metastatic cascade and thus improve our understanding of the molecular wiring of therapeutic responses. …”
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The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation by Malihe Moradzadeh, Sirous Tayebi, Hossein Poustchi, Kourosh Sayehmiri, Parisa Shahnazari, Elnaz Naderi, Ghodratollah Montazeri, Ashraf Mohamadkhani

“…Fifty-one patients with chronic hepatitis B, negative for HBeAg and detectable HBV DNA, were examined for the presence of mutations in pre-core region of HBV genome by direct sequencing. …”
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Identification of Potential Serum Metabolic Biomarkers of Diabetic Kidney Disease: A Widely Targeted Metabolomics Study by Hang Zhang, Jing-jing Zuo, Si-si Dong, Yuan Lan, Chen-wei Wu, Guang-yun Mao, Chao Zheng

“…Comprehensive analysis of metabolomics characteristics was conducted to detect the candidate metabolic biomarkers and assess their capability and feasibility. …”
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Unveiling the UNESCO Biosphere Reserve of the Berlengas Archipelago in Portugal as a Hotspot of Fish Species Using eDNA Metabarcoding and the Collaboration of Fishing Crews by Marco Simões, Cátia Costa, Maria da Luz Calado, Nuno Vasco-Rodrigues, Maria Jorge Campos, Sérgio Miguel Leandro, Agostinho Antunes

“…Fisher-collected samples were generally less diverse than those by the research team but detected species typical of deeper and open-ocean habitats, validating this sampling method. …”
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Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique andof probabilities of measurable concentrations of interleukins IL-2, IL-4, IL-5,... by Klabusay Martin, Kohutova Viera, Coupek Petr, Nenickova Marie, Tesarova Eva

“…Authors used the ELISA method to detect soluble cytokines and costimulatory molecules simultaneously in the plasma of 30 blood donors. …”
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Presence of dengue virus RNA in urine and oral fluid of laboratory-confirmed dengue patients: Implications for wastewater surveillance by Christine Stauber, Leile Camila Jacob-Nascimento, Caroline Grosch, Moisés da Silva Sousa, Moyra M. Portilho, Rosângela O. Anjos, Margo A. Brinton, Uriel Kitron, Mitermayer G. Reis, Guilherme S. Ribeiro

“…Of 53 serum RT-qPCR-positive patients, 6 (11.3 %) showed detectable DENV RNA in acute- or convalescent-phase urine. …”
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Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania by Agricola Joachim, Sabrina J. Moyo, Lillian Nkinda, Mtebe Majigo, Sima Rugarabamu, Elizabeth G. Mkashabani, Elia J. Mmbaga, Naboth Mbembati, Said Aboud, Eligius F. Lyamuya

“…A high proportion of MRSA carriage was detected among nurses (35, 45.5%). MRSA isolates showed high resistance toward kanamycin (83.7%), gentamicin (83.1%), ciprofloxacin (71.2%), and trimethoprim-sulphamethoxazole (46.8%) compared to methicillin-sensitive S. aureus isolates (p≤0.001). …”
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The Pro12Ala Polymorphism of PPAR-γ Gene Is Associated with Sepsis Disease Severity and Outcome in Chinese Han Population by Guoda Ma, Haiyang Wang, Guixi Mo, Lili Cui, You Li, Yiming Shao, Xin Liu, Yuliu Xie, Jia Li, Jiawu Fu, Hua Tao, Bin Zhao, Liangqing Zhang, Keshen Li

“…Genotyping was performed using the polymerase chain reaction-ligation detection reaction (PCR-LDR) method. No significant differences were detected in the allele and genotype distributions of the PPAR-γ Pro12Ala SNP between septic patients and controls (P=0.622 for genotype; P=0.629 for allele). …”
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Decoding the m6A epitranscriptomic landscape for biotechnological applications using a direct RNA sequencing approach by Chuwei Liu, Heng Liang, Arabella H. Wan, Min Xiao, Lei Sun, Yiling Yu, Shijia Yan, Yuan Deng, Ruonian Liu, Juan Fang, Zhi Wang, Weiling He, Guohui Wan

“…Traditional computational methods for detecting m6A from Nanopore direct RNA sequencing (DRS) data are constrained by their reliance on experimentally validated labels, often resulting in the underestimation of modification sites. …”
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Three-Dimensional Assessment of Dental Enamel Microcrack Progression After Orthodontic Bracket Debonding Using Optical Coherence Tomography by Ahmed Haj Hamdan, Sm Abu Saleah, Daewoon Seong, Naresh Kumar Ravichandran, Ruchire Eranga Wijesinghe, Sangyeob Han, Jeehyun Kim, Mansik Jeon, Hyo-Sang Park

“…Three-dimensional OCT imaging monitored existing EMCs at higher risk of progression and detected new EMCs following orthodontic bracket debonding. …”
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The first two mitochondrial genomes for the genus Ramaria reveal mitochondrial genome evolution of Ramaria and phylogeny of Basidiomycota by Qiang Li, Lijiao Li, Ting Zhang, Peng Xiang, Qian Wu, Wenying Tu, Zhijie Bao, Liang Zou, Cheng Chen

“…A total of 4499 bp and 7746 bp aligned fragments were detected between the mitochondrial and nuclear genomes of R. cfr. rubripermanens and R. rubella, respectively, indicating possible gene transferring events. …”
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Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea by Lu Zhang, Jun Bai, Qian Guo, Long Li, Yanqing Jia, Xinxin Qiu, Dong Zhou, Zhencang Zhang, Huafeng Niu

“…Additionally, antibiotic resistance genes for quinolones, β-lactams, chloramphenicol, tetracyclines, and aminoglycosides were detected, with significantly higher prevalence in diarrheic calves. …”
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Reduced frontotemporal connectivity during a verbal fluency task in patients with anxiety, sleep, and major depressive disorders by Fanxi Ding, Yiyang Ying, Yuqing Jin, Xuanru Guo, Xuanru Guo, You Xu, Zhenghe Yu, Haiteng Jiang, Haiteng Jiang, Haiteng Jiang

“…Previous studies have investigated the verbal fluency task (VFT) for detecting executive function impairment in different psychiatric disorders, but the sensitivity and specificity of this task in different psychiatric disorders have not been explored. …”
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Diaphragmatic crus indentation to the renal artery: Is it a new etiology for renovascular hypertension in adults? by Dilek Akkurt Acar, Atilla Hikmet Çilengir, Mehtap Balaban, Eren Çamur, Betül Akdal Dölek, Nilgün Işıksalan Özbülbül

“…Diaphragmatic crus indentation (DCI) was detected in 8.4% (n=21) of all patients, and 76.2% (n=16) of these were men. …”
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Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis by K. T. Sultankulova, O. V. Chervyakova, N. S. Kozhabergenov, K. A. Shorayeva, V. M. Strochkov, M. B. Orynbayev, N. T. Sandybayev, A. R. Sansyzbay, A. V. Vasin

“…The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. The tests were evaluated with use of 185 specimens from humans, animals, and birds. …”
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Tuberculosis and drug resistance in a region of Southern Italy among native and foreign-born populations: A twelve-year province-based study by Salvatore Rotundo, Salvatore Nisticò, Helen Linda Morrone, Luigia Gallo, Saveria Dodaro, Carmelo Papola, Pasquale Minchella, Giovanni Matera, Francesca Greco, Luigi Principe, Lorenzo Antonio Surace, Francesco Lucia, Francesca Serapide, Alessandro Russo, Carlo Torti, Enrico Maria Trecarichi

“…Tuberculosis (TB) remains a significant global health challenge, with the World Health Organization (WHO) aiming for a 95% reduction in TB deaths by 2030. Disparities in TB detection persist, particularly regarding gender, immigration status, and resistance patterns. …”
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Future changes in compound explosive cyclones and atmospheric rivers in the North Atlantic by F. Lopez-Marti, F. Lopez-Marti, M. Ginesta, D. Faranda, A. Rutgersson, A. Rutgersson, P. Yiou, L. Wu, G. Messori, G. Messori, G. Messori

“…To accomplish this, we independently detect and track atmospheric rivers and extratropical cyclones and study their concurrence in both ERA5 reanalysis and CMIP6 historical and future climate simulations. …”
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Predictive Value of Systemic Immune Inflammation Index for Postoperative Atrial Fibrillation in Patients Undergoing Isolated Coronary Artery Bypass Grafting by Murat Selçuk, Tufan Çınar, Faysal Şaylık, Selami Doğan, Ismail Selçuk, Ahmet L. Orhan

“…The AUC value of SII in detecting PoAF was much greater than the AUC values of both the neutrophil to lymphocyte ratio (NLR) and the platelet to lymphocyte ratio (PLR) (AUC: 0.6740 and AUC: 0.6426, respectively). …”
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Evaluating the toxicity of sea-dumped conventional and chemical munition degradation products to fish and human cells using a combination of cell viability assays by João Barbosa, Colin R. Janssen, Marijke Neyts, Koen Parmentier, Frédéric Laduron, Kris Geukens, Philippe François, Jana Asselman

“…Notably, the EC10 and EC50 values for tetryl and 1,3-DNB in RTgill-W1 align with concentrations detected near North American dumpsites, reflecting environmentally relevant conditions. …”
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Prevalence of <i>Batrachochytrium dendrobatidis</i> in Amphibians in Northwestern Italy’s Protected Areas by Arianna Meletiadis, Matteo Riccardo Di Nicola, Stefano Bovero, Marco Favelli, Marzia Pezzolato, Stefania Grella, Giusi Rezza, Pier Luigi Acutis

“…In the spring of 2023, Bd presence was detected in different amphibian species within two protected areas near Turin, Piedmont, Italy, following an unusual mortality event among the common toad (<i>Bufo bufo</i>). …”
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