Assessment of upper respiratory and gut bacterial microbiomes during COVID-19 infection in adults: potential aerodigestive transmission by Hafez Al-Momani, Andrew Nelson, Hadeel Al Balawi, Dua’a Al Balawi, Iman Aolymat, Ashraf I. Khasawneh, Hala Tabl, Ayman Alsheikh, AbdelRahman M. Zueter, Jeffrey Pearson, Christopher Ward

“…A significantly higher RSI score and salivary pepsin level were detected among those with COVID-19. The data indicates that COVID-19 is associated with a dysregulation of both the gut and lung microbiome with a more marked perturbation in the lung, particularly among COVID-19 patients who had been admitted to the ICU. …”
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The direct and urinary electrolyte-mediated effects of ambient temperature on population blood pressure: A causal mediation analysis by Ayesha Mukhopadhyay, Momenul Haque Mondol, Mahbubur Rahman, Leanne Unicomb, Rizwana Khan, Hoimonty Mazumder, Mohammad Nahian Ferdous, Emily V. Pickering, Konstantinos C. Makris, Alberto J. Caban-Martinez, Faruk Ahmed, Mohammad Shamsudduha, Fawaz Mzayek, Chunrong Jia, Hongmei Zhang, Anwar Musah, Lora E. Fleming, Matthew P. Smeltzer, Howard H. Chang, John L. Jefferies, Csaba P. Kovesdy, Xichen Mou, Abu Mohd Naser

“…For the combined mediators (sodium, potassium, calcium, and magnesium), for every 5°C increase in average daily temperature: the direct effect on systolic BP was −1.42 (95 % CI: −1.94, −0.92) mmHg and urine sodium mediated effect was −0.12 (95 % CI: −0.20, −0.05) mmHg; while urine potassium mediated effect was 0.15 (95 % CI: 0.08, 0.25) mmHg; urine calcium-mediated effect 0.06 (95 % CI: 0.01, 0.12) mmHg; and urine magnesium mediated effect −0.00 (95 % CI: −0.03, 0.02) mmHg. We detected similar associations for diastolic BP, pulse pressure, and mean arterial pressure. …”
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Investigation of vitamin D deficiency in girls with growth and development variations—a single center study by Panwang Huang, Panwang Huang, Beilei Zeng, Beilei Zeng, Feng Ren, Yuan Zhou, Yuan Zhou, Ye Li, Ye Li, Yinyin Huang, Yinyin Huang, Xingyu Liu, Xingyu Liu, Jiaxiu Zhou, Jiaxiu Zhou, Yaping Ma, Yaping Ma

“…PurposeTo understand the status of vitamin D deficiency in girls with growth and developmental variations, as well as the impact of COVID−19 on their vitamin D levels, and to provide reference for the prevention and treatment of vitamin D deficiency in children.MethodsA retrospective analysis was conducted on 1,345 instances of girls with growth and developmental variations who visited our pediatric endocrinology department and completed vitamin D detection. A total of 279 girls with complete data were included in this study. …”
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Mycotoxilogical monitoring. Part 2. Wheat, barley, oat and maize grain by G. P. Kononenko, A. A. Burkin, Ye. V. Zotova

“…Fusarium toxins including T-2 toxin, diacetoxyscirpenol, deoxynivalenol, zearalenone and fumonisins of group B as well as alternariol, ochratoxin A, citrinin, aflatoxin В1, sterigmatocystin, cyclopiazonic acid, mycophenolic acid, ergot alkaloids and emodin were detected and measured according to the validated competitive ELISA procedure. …”
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Calf immunization protocols with low-virulence isolates of Anaplasma marginale: Analysis of post-inoculation effects and protection against natural challenge by Filipe Lucas de Melo Mendonça, Matheus Figueiredo Coelho, Camila Valgas Bastos, Júlia Angélica Gonçalves da Silveira, Rafael Romero Nicolino, Jose Azael Zambrano Uribe, Múcio Flávio Barbosa Ribeiro, Bruna Torres Silvestre, Rodrigo Melo Meneses, Antônio Último de Carvalho, Tiago Facury Moreira, Elias Jorge Facury-Filho

“…There were no significant differences in rickettsemia levels, reduction in PCV, or antibody detection among the different inoculation strategies. …”
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Psychometric properties and meaningful change thresholds for the QOL-E instrument in patients with myelodysplastic neoplasms by Esther Natalie Oliva, Shien Guo, Jennifer Lord-Bessen, Aylin Yucel, Roberto Latagliata, Massimo Breccia, Giuseppe A. Palumbo, Grazia Sanpaolo, Marta Riva, Valeria Santini, Uwe Platzbecker, Guillermo Garcia-Manero, Pierre Fenaux, Christopher G. Pelligra

“…The QOL-E domains/summary scores, except for QOL-SEX, had an adequate ability to detect change from baseline to Week 24. MWPC thresholds were proposed for all other domains and summary scores.ConclusionThe study results demonstrate that the QOL-E is generally fit for purpose to assess treatment effects in populations with LR-MDS and the proposed MWPC thresholds can be used to assess within-patient treatment effect on PROs, as assessed by the QOL-E, in future studies.…”
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A multidimensional assessment of adverse events associated with paliperidone palmitate: a real-world pharmacovigilance study using the FAERS and JADER databases by Siyu Lou, Zhiwei Cui, Yingyong Ou, Junyou Chen, Linmei Zhou, Ruizhen Zhao, Chengyu Zhu, Li Wang, Zhu Wu, Fan Zou

“…Furthermore, gender-based difference in risk signals was detected. Females are more likely to experience elevated blood prolactin and weight increase, whereas males are more prone to sexual dysfunction. …”
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Yishenyangsui granule for degenerative cervical myelopathy: a randomized, double-blind, placebo-controlled trial with long-term follow-up by He Yin, Xin Chen, Zhiwei Liu, Zhiwei Liu, Bo Xu, Zhefeng Jin, Yan Liu, Baoyu Qi, Bin Tang, Ping Wang, Fanping Xu, Xu Wei, Jie Yu, Liguo Zhu, Liguo Zhu

“…However, there was a significant difference detected in re-treatment data between groups with a lower rate observed among those receiving Yishenyangsui granule compared to those receiving placebo [25 (43.10%) vs. 40 (68.97%); P = 0.033], indicating its effectiveness for treating mild-to-moderate Degenerative Cervical Myelopathy.ConclusionYishenyangsui granule was effective in treating mild to moderate Degenerative Cervical Myelopathy. …”
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Clinical and microbiological analyses of colistin-resistant strains among carbapenem-resistant Enterobacter cloacae complex clinical isolates by Jinyoung Yang, Jin Yang Baek, Jae-Hoon Ko, Kyungmin Huh, Sun Young Cho, Doo Ryeon Chung, Kyong Ran Peck, Hee Jae Huh, Kwan Soo Ko, Cheol-In Kang

“…A total of 73 sequence types (STs), including 28 newly identified STs, were detected, demonstrating significant clonal diversity. …”
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Health checks for autistic adults: study protocol for a cluster randomised controlled trial by Jeremy R. Parr, Helen Taylor, Colin Wilson, Clare Scarlett, Sarah Al-Asmori, Carole Buckley, Sally-Ann Cooper, Cristina Fernandez-Garcia, Tracy Finch, Rhianna Lees, Nicholas Lennox, Hannah Merrick, Sebastian Moss, Christina Nicolaidis, Malcolm Osbourne, Dora M. Raymaker, Tomos Robinson, Anna Urbanowicz, James M. S. Wason, Barry Ingham

“…The primary outcome will be the incidence of new health needs/conditions detected and met at 9 months (data gathered from participant’s GP records). …”
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Clinical expression of facioscapulohumeral muscular dystrophy in carriers of 1–3 D4Z4 reduced alleles: experience of the FSHD Italian National Registry by Antonio Di Muzio, Corrado Angelini, Tiziana Mongini, Liliana Vercelli, Lucia Morandi, Giovanni Antonini, Gabriele Siciliano, Lucio Santoro, Maurizio Moggio, Giacomo Brisca, Luisa Villa, Giuliano Tomelleri, Francesco Sera, Carmelo Rodolico, Lorenzo Maggi, Giulia Ricci, Angela Berardinelli, Elena Pegoraro, Sabrina Ravaglia, Chiara Fiorillo, Michelangelo Cao, Rossella Tupler, Ana Nikolic, Elisabetta Bucci, Monica Govi, Fabiano Mele, Marta Rossi, Lucia Ruggiero, Maria Chiara D'Amico, Maria Grazia D'Angelo, Claudio Bruno

“…Comparison of the disease severity outcome between index cases with age at onset before and over 10 years of age, failed to detect statistical significance (Wald test p value=0.064). …”
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Genetic Predisposition and Salt Sensitivity in a Chinese Han Population: The EpiSS Study by Kuo Liu, Bo Xi, Zheng Liu, Han Qi, Bin Liu, Jie Zhang, Han Cao, Yuxiang Yan, Min Zhao, Yan He, Ling Zhang

“…A modified Sullivan’s acute oral saline load and diuresis shrinkage test was used to detect salt sensitivity. Logistic regression was used to estimate the joint effect of the SNPs on SS both overall and after stratification by hypertension. …”
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Prevalence, Incidence and Predictors of Anal HPV Infection and HPV-Related Squamous Intraepithelial Lesions in a Cohort of People Living with HIV by Margherita Sambo, Alessandra Bailoni, Federico Mariani, Massimo Granai, Natale Calomino, Virginia Mancini, Anna D’Antiga, Francesca Montagnani, Mario Tumbarello, Stefano Lazzi, Franco Roviello, Massimiliano Fabbiani

“…<b>Background:</b> Anal HPV infection can cause squamous intraepithelial lesions (SILs), which are precursors of anal squamous cell carcinoma (SCC). The early detection of HPV infections and improvement of effective screening programmes are, therefore, essential to prevent progression from pre-cancerous lesions to SCC, especially in people living with HIV (PLWH), who represent a population at higher risk of HPV infection and associated lesions. …”
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Impact of using time-averaged exposure metrics on binary endpoints in exposure-response analyses by Yu-Wei Lin, Yu-Wei Lin, Yu-Wei Lin, Yu-Wei Lin, Yu-Wei Lin, Anna Largajolli, Anna Largajolli, A. Yin Edwards, A. Yin Edwards, S. Y. Amy Cheung, S. Y. Amy Cheung, Kashyap Patel, Stefanie Hennig, Stefanie Hennig

“…The derivation of CavTE at different time points demonstrated significant impact on trends detected in logistic ER relationships that could bias subsequent event projection, dose selection and Go/No-Go decisions. …”
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Significance of KLK7 expression, polymorphisms, and function in sheep horn growth by Mingzhu Shan, Hao Li, Xinyue Li, Guoqing Zhang, Jianxin Shi, Pingjie Feng, Lisheng Zhou, Zhangyuan Pan, Mingxing Chu

“…Finally, the WGS data were linked with sheep horn length for association analysis, and significantly different single-nucleotide polymorphisms (SNPs) were detected and validated by Kompetitive Allele Specific PCR (KASP) genotyping. …”
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SARS-CoV-2 infection and antibody seroprevalence in routine surveillance patients, healthcare workers and general population in Kita region, Mali: an observational study 2020–2021... by Ulla Ashorn, Per Ashorn, Nigel Klein, Samba O Sow, Camilla Ducker, Elaine Cloutman-Green, Dagmar Alber, Fadima Cheick Haidara, Juho Luoma, Laura Adubra, Henry Badji, Fatoumata Diallo, Rikhard Ihamuotila, Owen Martell, Uma U Onwuchekwa, Oumar Samaké, Awa Traore, Kevin Wilson, Yue-Mei Fan

“…Objective To estimate the degree of SARS-CoV-2 transmission among healthcare workers (HCWs) and general population in Kita region of Mali.Design Routine surveillance in 12 health facilities, HCWs serosurvey in five health facilities and community serosurvey in 16 villages in or near Kita town, Mali.Setting Kita region, western Mali; local health centres around the central (regional) referral health centre.Participants Patients in routine surveillance, HCWs in local health centres and community members of all ages in populations associated with study health centres.Main outcome measures Seropositivity of ELISA test detecting SARS-CoV-2-specific total antibodies and real-time RT-PCR confirmed SARS-CoV-2 infection.Results From 2392 routine surveillance samples, 68 (2.8%, 95% CI: 2.2% to 3.6%) tested positive for SARS-CoV-2 by RT-PCR. …”
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Impaired hemodynamic forces assessed by routine CMR and its determinants in different duration T2DM patients with normal LV function and myocardial strain by Guozhu Shao, Guozhu Shao, Guozhu Shao, Yukun Cao, Yukun Cao, Yukun Cao, Yue Cui, Yue Cui, Yue Cui, Hongyan Li, Heshui Shi, Heshui Shi, Heshui Shi

“…BackgroundEarly detection of subclinical myocardial dysfunction in asymptomatic patients with type 2 diabetes mellitus (T2DM) is essential before overt changes in left ventricular ejection fraction (LVEF) and myocardial strain occur. …”
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Short-term variability of Alzheimer’s disease plasma biomarkers in a mixed memory clinic cohort by Frederikke Kragh Clemmensen, Mathias Holsey Gramkow, Anja Hviid Simonsen, Nicholas J. Ashton, Hanna Huber, Kaj Blennow, Henrik Zetterberg, Gunhild Waldemar, Steen Gregers Hasselbalch, Kristian Steen Frederiksen

“…Secondly, we established the minimum change required to detect a difference between two given blood samples that exceeds intra-individual variability and analytical variation (reference change value, RCV). …”
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Integrated metabolome and transcriptome analysis reveals key genes and pathways associated with egg yolk percentage in chicken by Wen Li, Yuxing Luo, Shoujia Zhu, Mengyuan Wang, Xuli Zhao, Zhonghua Ning

“…A total of 322 differentially expressed genes (DEGs) and 128 significantly differential metabolites (SDMs) (VIP>1, P < 0.05) were identified in the liver, whereas 419 DEGs and 215 SDMs were detected in the ovary, and 238 DEGs along with 47 SDMs were found in the magnum. …”
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Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies by Camilla T. Ekanger, Camilla T. Ekanger, Camilla T. Ekanger, Nilima Dinesh Kumar, Rosanne W. Koutstaal, Fan Zhou, Martin Beukema, Joanna Waldock, Simon P. Jochems, Noa Mulder, Cécile A. C. M. van Els, Cécile A. C. M. van Els, Othmar G. Engelhardt, Nathalie Mantel, Kevin P. Buno, Karl Albert Brokstad, Karl Albert Brokstad, Agnete S. T. Engelsen, Agnete S. T. Engelsen, Rebecca J. Cox, Rebecca J. Cox, Barbro N. Melgert, Barbro N. Melgert, Anke L. W. Huckriede, Puck B. van Kasteren

“…Following infection, a reproducible antiviral cytokine response, including a consistent increase in CXCL10, IL-6, IFN-λ1, IFN-λ2/3, and IFN-β, was detected across all models. Finally, neutralization was assessed by pre-incubation of virus with human serum. …”
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