PIVKA-II as a surrogate biomarker for therapeutic response in Non-AFP-secreting hepatocellular carcinoma by San-Chi Chen, Hsiang-Ling Ho, Chien-An Liu, Yi‑Ping Hung, Nai-Jung Chiang, Ming‑Huang Chen, Yee Chao, Muh-Hwa Yang

“…Prothrombin induced by vitamin K absence II (PIVKA-II) is more sensitive for HCC detection, though its role in systemic therapy remains underexplored. …”
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The correlation of the forkhead transcription factor foxo1 expression and histopathological pancreatic lesions in dogs by Firas M. Abed, Ahmed N. Abduljawaad, Omar A. Al-Mahmood, Karam H. Yehia, Hana Kh. Ismail

“…Among the 14 samples, degenerative changes, congestion, edema, coagulative and fat necrosis, as well as inflammation were detected in some samples. Multiple lesions, including fibrosis, hyperplasia, and atrophy, were observed in other samples. …”
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Proinflammatory Cytokine IL-6 and JAK-STAT Signaling Pathway in Myeloproliferative Neoplasms by Vladan P. Čokić, Olivera Mitrović-Ajtić, Bojana B. Beleslin-Čokić, Dragana Marković, Marijana Buač, Miloš Diklić, Nada Kraguljac-Kurtović, Svetozar Damjanović, Pavle Milenković, Mirjana Gotić, Puri K. Raj

“…Moreover, thrombocytosis was reduced by JAK2V617F allele burden in essential thrombocythemia (ET) and PMF. 261 significantly changed genes have been detected in PV, 82 in ET, and 94 genes in PMF. The following JAK-STAT signaling pathway related genes had augmented expression in CD34+ cells of MPNs: CCND3 and IL23A regardless of JAK2V617F allele burden; CSF3R, IL6ST, and STAT1/2 in ET and PV with JAK2V617F mutation; and AKT2, IFNGR2, PIM1, PTPN11, and STAT3 only in PV. …”
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Effects of asiaticoside on the model of gestational diabetes mellitus in HTR-8/svneo cells via PI3K/AKT pathway by Zhilan Hu, Ya Long, Xiangyue Li, Zhiqin Jia, Mingyan Wang, Xuemei Huang, Xiaolan Yu

“…The cell proliferation and migration were detected by CCK-8 assay and scratch test, respectively. …”
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Breeding for sustainability: how reproductive biotechnologies can help buffalo farmers combat climate change by Pietro Sampaio Baruselli, Laís Ângelo de Abreu, Vanessa Romário de Paula, Sofía Albertini, Guilherme Felipe Ferreira dos Santos, Lígia Mattos Rebeis, Emanuelle Almeida Gricio, Nelcio A.T. de Carvalho, Otavio Bernardes

“…Furthermore, it allows fixed-time embryo transfer in recipients, with high efficiency and no need for estrus detection. Thus, ARTs, such as AI and ET, are applied for buffalo’s targeted multiplication and dispersal with defined production and environmental credentials. …”
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Comparative analysis of EGFR mutations in circulating tumor DNA and primary tumor tissues from lung cancer patients using BEAMing PCR by Duhita Mirikar, Nandini Banerjee, Kumar Prabhash, Rajiv Kumar Kaushal, Vanita Naronha, C. S. Pramesh, George Karimundackal, Amit Joshi, Swapnil Rane, Ranjan Basak

“…Noninvasive mutation detection by assessing circulating tumor DNA (ctDNA) offers many advantages over tumor biopsy. …”
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A SARS‐CoV‐2 EG.5 mRNA vaccine induces a broad‐spectrum immune response in mice by Hongyu Wang, Qinhua Peng, Xinxian Dai, Zhifang Ying, Xiaohong Wu, Xinyu Liu, Hongshan Xu, Jia Li, Leitai Shi, Jingjing Liu, Yunpeng Wang, Danhua Zhao, Yanqiu Huang, Lihong Yang, Ren Yang, Guangzhi Yue, Yue Suo, Qiang Ye, Shouchun Cao, Yuhua Li

“…Meanwhile different immunization strategies and doses were performed to detect the immunogenicity of this mRNA vaccine. Our results show that two doses of 5 µg CoV072 or a single dose of 15 µg CoV072 both induced broad‐spectrum cross‐protection ability in mice. …”
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Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepe... by O. P. Gavrilova, T. Yu. Gagkaeva, A. S. Orina, A. S. Markova, A. D. Kabashov, I. G. Loskutov

“…The DNA amount of toxin-producing species from genus Fusarium Link in the grain was detected using the real-time PCR, and the amount of mycotoxins was measured by the enzyme immunoassay.Results. …”
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Retrospective Investigation of Cytomegalovirus and Epstein–Barr Virus Positivity in Inflammatory Bowel Disease Patient Biopsies by Hasan Bostancı, Kürşat Dikmen, Ahmet Çağrı Büyükkasap, Sidre Erganis, Kayhan Çağlar, Ayşe Dursun, Gülendam Bozdayı

“…EBV positivity in the control group was 16%, and CMV was not detected. The presence of EBV and CMV in CD (p=0.049; p=0.024) and in UC (p=0.013; p=0.001) patients was statistically significantly higher than that in the control group. …”
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Automated diagnosis and classification of metacarpal and phalangeal fractures using a convolutional neural network: a retrospective data analysis study by Michael Axenhus, Anna Wallin, Jonas Havela, Sara Severin, Ablikim Karahan, Max Gordon, Martin Magnéli

“…The use of artificial intelligence (AI) in fracture detection has shown promise, but research focusing on hand metacarpal and phalangeal fractures remains limited. …”
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Discovery of conserved peptide-MHC epitopes for directly alloreactive CD8+ T cells by Alexandra E. Hill, Eric T. Son, Moumita Paul-Heng, Chuanmin Wang, Shivanjali Ratnaseelan, Martina Denkova, Pouya Faridi, Asolina Braun, Anthony W. Purcell, Nicole A. Mifsud, Alexandra F. Sharland

“…Eight peptide-Kd epitopes were selected for inclusion in a tetramer panel to detect directly alloreactive CD8+ T cells. This panel bound over 30% of activated alloreactive CD8+ T cells after a prime-boost against Kd. …”
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Significance of Endoscopic Redness of Duodenum in Health Checkup by Atsushi Nakayama, Motohiko Kato, Yoshiki Sakaguchi, Yu Takahashi, Shinya Kodashima, Ai Fujimoto, Nobutake Yamamichi, Kazumasa Miki, Naohisa Yahagi

“…Conclusion To evaluate the difference in the degree of redness between WLI and LCI of duodenal bulb in esophagogastroduodenoscopy may help in early detection of metabolic syndrome, which rarely has symptoms.…”
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Event-Based Slip Estimation Framework for Space Rovers Traversing Soft Terrains by Ruqayya Alhammadi, Yahya Zweiri, Ahmad Abubakar, Mubarak Yakubu, Laith Abuassi, Lakmal Seneviratne

“…These limitations prevent the immediate detection of slip events, which can occur in microseconds, making such methods unsuitable for highly variable terrain environments such as lunar surfaces. …”
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Adverse childhood experiences and health outcomes: a 20-year real-world study by Bárbara Martins, Tiago Taveira-Gomes, Tiago Taveira-Gomes, Tiago Taveira-Gomes, Joana Costa Gomes, Maria João Vidal-Alves, Maria João Vidal-Alves, Teresa Magalhães, Teresa Magalhães, Teresa Magalhães, Teresa Magalhães

“…Social problems, injuries and intoxications, mental health disorders, and physical disorders were observed at higher percentages in suspected victims than in the total analyzed population.DiscussionThese results reveal that child victims of ACEs may be underdiagnosed, which, given the aforementioned (and described in the literature) severe consequences for their current and future health, should be taken as a critical warning for healthcare professionals. Detections and reports are fundamental for early treatment, aiming to avoid an escalation of damage and prevent re-victimization.…”
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Activation of Opioid Receptors Attenuates Ischemia/Reperfusion Injury in Skeletal Muscle Induced by Tourniquet Placement by Yue-Xian Guo, Gui-Ying Wang, Wen-jie Cheng, Cai-Zhen Yan, Shuang Zhao, Zhao Li, Peng Liu, Xiu-Li Wang

“…Expression levels of TLR4, NF-κB, SIRT1, and PGC-1α in the skeletal muscles were detected by western blot. Blood TNF-α levels, gastrocnemius muscle contractile force, and ATP concentration were examined. …”
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Assessment of Fish Species Biodiversity in the Yong River Basin Based on Environmental DNA Metabarcoding by Shengming Deng, Bai-an Lin, Yan Luo, Xiaolong Dang, Chengdan Ma, Yongcan Zhou, Xiang Zhang, Yang Zhang, Ning Xu, Shousong Jiang, Jiana Li, Jianlong Li

“…However, a decline in species biodiversity was observed in the whole Yong River basin, with a significant difference in community composition across the Upper Yao River (UYAR) and Yong River (YOR). Moreover, eDNA detected fish and potentially invasive species consistent with their geographic distribution. …”
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Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC by Yujie Ai, Zhi Yu, Yuqiong Chen, Xiaojing Zhu, Zeyi Ai, Shuyuan Liu, Dejiang Ni

“…TPS sample was hydrolyzed with trifluoroacetic acid, subjected to pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone (PMP), and separated on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with UV detection at 250 nm. A mixture of ten PMP derivatives of standard monosaccharides (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, arabinose, and fucose) could be baseline separated within 20 min. …”
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Triglyceride glucose-waist circumference as a useful predictor for diabetes mellitus: a secondary retrospective analysis utilizing a Japanese cohort study by Yawei Li, Linlin Shan, Qiong Wen, Changchun Cao, Meiling Huang, Chunxia Zhang, Xiaoping Li, Kun Wang, Tianlun Zhou, Fubing Zha, Yulong Wang

“…Furthermore, a Receiver Operating Characteristic (ROC) curve was created to detect the predictive capability of TyG-WC for diabetes risk. …”
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Molecular assessment of splicing variants in a cohort of patients with inborn errors of immunity: methodological approach and interpretation remarks by Laura Miguel Berenguel, Carla Gianelli, Carla Gianelli, Carla Gianelli, Elisabet Matas Pérez, Teresa del Rosal, Ana Méndez Echevarría, Ángel Robles Marhuenda, Marta Feito Rodríguez, Maria Teresa Caballero Molina, Maria Teresa Caballero Molina, Lorena Magallares García, Brenda Sánchez Garrido, Samantha Hita Díaz, Luis Allende Martínez, Luis Allende Martínez, Pilar Nozal Aranda, Pilar Nozal Aranda, Pilar Nozal Aranda, Carmen Cámara Hijón, Carmen Cámara Hijón, Carmen Cámara Hijón, Eduardo López Granados, Eduardo López Granados, Eduardo López Granados, Rebeca Rodríguez Pena, Rebeca Rodríguez Pena, Rebeca Rodríguez Pena, María Bravo García-Morato, María Bravo García-Morato, María Bravo García-Morato

“…Reverse transcription-polymerase chain reaction (RT-PCR) is the molecular assay employed for pathogenicity validation.ResultsWe detected 23 patients of 20 pedigrees with splicing variants in IEI genes, which constitutes the 12.3% of our cohort. …”
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Feasibility Study of the Use of Handheld NIR Spectrometer for Simultaneous Authentication and Quantification of Quality Parameters in Intact Pineapple Fruits by Charles L. Y. Amuah, Ernest Teye, Francis Padi Lamptey, Kwasi Nyandey, Jerry Opoku-Ansah, Peter Osei-Wusu Adueming

“…The results generally indicated that portable NIR spectrometer coupled with the appropriate chemometric tools could be employed for rapid nondestructive examination of pineapple quality and also to detect pineapple fraud due to mislabeling of conventionally produced fruits as organic ones. …”
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