Encapsulation of a Pediocin PA-1 producer Pediococcus acidilactici and its impact on enhanced survival and gut microbiota modulation by M. Mokrani, A. Elie, C. Jacquot, F. Kiran, M. Urdaci

“…Furthermore, the impact of either encapsulated (E) or non-encapsulated (NE) P. acidilactici in mice gut microbiota was determined using quantitative Polymerase Chain Reaction (qPCR) and Denaturing Gradient Gel Electrophoresis (DGGE). Results: Mass spectrometry analysis and the sequence of the amplified pediocin gene identified the bacteriocin as a pediocin PA-1. …”
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Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process by Claudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas Filho

“…Burns are lesions in which the thermal energy of the causative agent transfers heat to the surface of the body, causing superficial or deep damage to the skin with protein denaturation in cells and biochemical maladjustments, which delay and disrupt the cicatricial process, increasing the chances of functional and aesthetic sequelae. …”
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Effect of HTST Thermal Treatments on End-Use Quality Characteristics of Goat Milk by B. D. Rohitha Prasantha, K. M. S. Wimalasiri

“…Total protein (TP%) and nonprotein nitrogen contents were stable IAP but TP reduced significantly (P<0.05) at two weeks storage. The whey protein denaturation increased with pasteurization treatments and storage time. …”
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INVESTIGATING THE BIOCHEMICAL PROFILE AND NUTRITIONAL COMPOSITION OF VERNONIA AMYGDALINA LEAVES: A STUDY ON ANTIOXIDANT PROPERTIES, PHYTOCHEMICAL COMPOSITION, ENZYME INHIBITION, AN... by Olugbenga D. Oloruntola, Fehintoluwa S. Oladebeye, Samuel A. Adeyeye, Simeon O. Ayodele, Ojurereoluwa A. Ayodele, Olufemi E. Adeniji

“…Moreover, the study unveiled the extract’s inhibitory effects on key enzymes and proteins involved in metabolic processes, with notable rates of 29.34% for anti-glucosidase activity, 43.28% for alpha-amylase inhibition, 34.44% for albumin denaturation inhibition, and 57.04% for anti-proteinase activity. …”
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Effects of Acid and Enzymatic Extraction on the Structure and Physicochemical Characterization of Collagen from Chinemys reevesii Skin by Dandan YU, Wenzhi WEI, Li GUAN, Wenzhuo CHEN, Hui CAO

“…ASC and PSC exhibited similar secondary structures, according to their comparable characteristic absorption peaks. The thermal denaturation temperatures of ASC and PSC were 27.21±0.28 ℃ and 28.37±0.23 ℃, respectively. …”
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Elucidating the role of carrier proteins in cytokine stabilization within double emulsion‐based polymeric nanoparticles by Emily R. Rhodes, Nicole B. Day, Emma C. Aldrich, C. Wyatt Shields IV, Kayla G. Sprenger

“…We utilized an integrated computational and experimental approach for studying the governing mechanisms by which interleukin‐12 (IL‐12), a clinically relevant cytokine, is protected from denaturation by albumin, a common stabilizing protein, at an organic‐aqueous solvent interface formed during double emulsification. …”
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Age and diet affect self-resilience of intestinal microbiome in mice by Chenyi Shao, Shenmin Chen, Huan Yang, Mufan Li, Yinhui Liu, Shu Wen, Jing Xiao, Li Tang

“…In addition, 16S rRNA sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis were performed to identify the bacterial taxa and microbiome structure, respectively.ResultsThe diversity of the gut microbiota in infant mice was recovered by the sixth week, while relative abundance of Ruminococcaceae_UCG_014 was low and did not return to normal levels. …”
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Effect of Carbonation on the Leachability of Solidified/Stabilized Lead-Contaminated Expansive Soil by Fusheng Zha, Congmin Liu, Bo Kang, Long Xu, Chengbin Yang, Chengfu Chu, Chuang Yu, Wei Zhang, Jiwen Zhang, Zhenghong Liu

“…Finally, with literature and experimental results, we found that the main reason for the increase of lead ion filtration of the carbonation reduced the pH value of the matrix and made the hydration products denatured and decomposed.…”
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Composition and Significance of Bacterial Microbiota and Volatile Organic Compounds of Swiss-Dutch-Type Cheese as Determined by PCR-DGGE and HS-GC by Beata Nalepa, Magdalena Anna Olszewska, Lidia Hanna Markiewicz, Marek Aljewicz

“…Bacterial diversity and VOCs (acetaldehyde; ketones: acetone, diacetyl, acetoin; alcohols: methanol, ethanol; esters: ethyl acetate, ethyl propionate, ethyl butyrate; fatty acids: acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, isocaproic acid, caproic acid, heptanoic acid) were determined by polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE), and headspace gas chromatography (HS-GC), respectively. …”
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Changes of Quality Characteristics before and after Embrittlement of Oreochromis niloticus×O. aureus by Mei CHEN, Jingkai QIN, Ziyi YANG, Zhongyi QIN, Jie ZHOU, Jiequn JIANG, Xiaoling LIU

“…The freezing point of CT was −0.81℃, lower than that of NT was −0.73 ℃, and the enthalpy of protein denaturation was 21.48% higher than that of NT. The muscle fiber structure of CT was more compact and dense, and the hardness, stickiness and mastication of CT were significantly higher than those of NT (P<0.05). …”
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Emulsifying Properties of Dried Soy-Whey, Dried Tofu-Whey, and Their Isolated Proteins by Sobral Pablo A., Ossa J. Sebastian Henao, Palazolo Gonzalo G., Wagner Jorge R.

“…Again, this improvement was more pronounced for samples obtained from soy-whey, probably due to partial protein denaturation associated to treatment with the organic solvent. …”
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T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses by Billaud, Maud, Theodorou, Ilias, Lamy-Besnier, Quentin, Shah, Shiraz A., Lecointe, François, De Sordi, Luisa, De Paepe, Marianne, Petit, Marie-Agnès

“…A more recent alternative permits to bypass the amplification step, as library adapters are ligated to sheared and denatured DNA, after an end-modification step (xGen kit). …”
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Comparative analysis of acids and temperatures in gelatin extraction from the skin of yellowfin tuna (Thunnus albacares) by Vicky Prajaputra, Sri Agustina, Nadia Isnaini, Agillia Nabila, Hikma Kasih Margolang, Sahra Apriani, Siti Maqfirah

“…Both acid-extracted gelatins showed α-chains and β-chains, characteristic of denatured collagen. In conclusion, acetic acid proved to be more effective than citric acid in producing higher yield and superior quality gelatin from yellowfin tuna skin. …”
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Preliminary Study by Differential Scanning Calorimetric Analysis of Red Blood Cells in Peripheral Artery Disease Patients Treated with Cilostazol: Correlation with Improvements in... by Dénes Lőrinczy, Dorottya Szabó, László Benkő

“…<b>Results</b>: The DSC analysis revealed shifts in thermal properties, including change in peak (melting or denaturation) temperature (<b>T_p</b>) and calorimetric enthalpy (<b>ΔH_cal</b>), which indicate significant structural changes in red blood cells. …”
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Biochemistry of the Thrombin-Like TLBpic and Its Purification from Bothrops pictus “Jergon de la Costa” (Reptilia: Viperidae) by Victor Ludgardo Álvarez Tohalino, Hugo Guillermo Jiménez Pacheco, Juan Raúl Jesús Zumaran Farfán, Pavel Kewin Delgado Sarmiento, Paulino Rodolfo Zegarra Panca

“…The ESI (electrospray ionisation), corresponding to the electrophoresis of proteins in polyacrylamide gels and to their denaturing solubilization in the presence of the SDS ionic detergent, uniting the proteins, breaking hydrophobic interactions, showing a molecular mass of ∼30 kDa, demonstrating high molecular homogeneity that exists in this family of proteins, is a soft ionization method, in which the samples were ionized by the addition or removal of a proton, with very little extra energy to cause fragmentation of the produced ions. …”
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Effects of L-carnitine and pentoxifylline on long-term preservation of the human sperms: An experimental study by Elham Aliabadi, Zohre Nateghian, Mohammad Hossein Nasr-Esfahani, Marziyeh Tavalaee, Tahereh Talaei-Khozani

“…However, DNA was resistant to denaturation regardless of the treatments. …”
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Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification by Jin-Ha Song, Seung-Eun Son, Ho-Won Kim, Seung-Ji Kim, Se-Hee An, Chung-Young Lee, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Kang-Seuk Choi, Kang-Seuk Choi

“…Our approach employs anti-NP monoclonal antibodies for the targeted isolation of RNA bound to RNPs, facilitating a quick and specific RNA extraction process that negates the need for centrifugation. Additionally, by denaturing the RT-RPA amplicon using 60% DMSO, we activate the trans-ssDNA cleavage activity of the Cas12a protein without the need for a specific PAM (5’-TTTV-3′) sequence. …”
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Deciphering In Vitro and In Vivo Pharmacological Properties of Seed and Fruit Extracts of Flacourtia jangomas (Lour.) Raeusch by Farhana Alam Ripa, Fowzia Alam, Fahmida Haque Riya, Yesmin Begum, Sharmin Akter Eti, Nusratun Nahar, Zebunnesa Ahmed, Sabrina Sharmin

“…The thrombolytic activity was assessed through rapid clot analysis of human blood samples, revealing that SFJC demonstrated the highest thrombolytic activity (60.99 ± 2.28%) compared to streptokinase (72.89 ± 2.19%). In the protein denaturation antiarthritic test, the PFJE and SFJC extracts exhibited significant potency, achieving results of 74.28 ± 1.16% and 79.25 ± 0.83%, respectively, at a dose of 500 μg/mL. …”
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Exploring the prevalence of gout among underrepresented low socioeconomic status type 2 diabetes populations by Jessica Smith, Abey Martin, Jane Mundadan, Michael Roberts, Youssef Roman, Arturo Bravo-Nuevo, Farzaneh Daghigh

“…Type 2 Diabetes is a condition characterized by the denaturation of the insulin receptors, due to chronically high blood glucose levels, leading to impaired regulation of blood sugar. …”
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A comparative analysis of alpha olefin sulfonate and dodecyl sulfate in aphronic fluid containing xanthan gum in a wide range of temperatures by Mohsen Riazi, Abdolhossein Hemmati-Sarapardeh, Mahin Schaffie, Reza Zabihi, Masoud Riazi

“…This difference can be attributed to SDS’s tendency to denature the XG polymer, leading to a notable decrease in viscosity. …”
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