Geometric Simulation Approach for Grading and Assessing the Thermostability of CALBs by B. Senthilkumar, D. Meshachpaul, R. Rajasekaran

“…Moreover, simulated thermal denaturation of CALB, a process involving dilution of hydrogen bond, significantly shielded against different intervals of energy application in mutant CALB revealing its augmentation of structural rigidity against native CALB. …”
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Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning by P. Janczyk, R. Pieper, V. Urubschurov, K. R. Wendler, W. B. Souffrant

“…Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. …”
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Anticancer, Antibacterial, Antioxidant, and DNA-Binding Study of Metal-Phenalenyl Complexes by Subhadeep Sen, Nilkanta Chowdhury, Tae-Wan Kim, Mohuya Paul, Dilip Debnath, Seob Jeon, Angshuman Bagchi, Jungkyun Im, Goutam Biswas

“…The DNA-binding properties of these metal-PLY complexes were investigated using absorption spectroscopy, fluorescence spectroscopy, viscosity measurements, and thermal denaturation methods. Experimental evidence revealed that the complexes bind to DNA through intercalation, and the molecular docking study supported this conclusion.…”
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Using Commercial Bio-Functional Fungal Polysaccharides to Construct Emulsion Systems by Associating with SPI by Laixin Dai, Qingfu Wang, Lining Wang, Qinghua Huang, Biao Hu

“…Heat treatment had a limited effect on electrostatically driven complexation but resulted in larger particles through a protein-denaturation-induced increase of hydrophobic interactions. …”
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Analysis of quality-related proteins in golden pompano (Trachinotus ovatus) fillets with modifi ed atmosphere packaging under superchilling storage by Chuang Pan, Xiaofan Zhang, Shengjun Chen, Yong Xue, Yanyan Wu, Yueqi Wang, Di Wang

“…Total sulfhydryl content, TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation. The Fresh (FS), MAP, and air packaging (AP) groups were set. …”
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Association of Xmn I Polymorphism and Hemoglobin E Haplotypes on Postnatal Gamma Globin Gene Expression in Homozygous Hemoglobin E by Supachai Ekwattanakit, Yuwarat Monteerarat, Suchada Riolueang, Kalaya Tachavanich, Vip Viprakasit

“…No association was found on a specific haplotype or Xmn I in these individuals with high Hb F, measured by alkaline denaturation. Conclusion. The cis-regulation of γ globin gene expression might not be apparent under a milder condition with lesser globin imbalance such as homozygous Hb E.…”
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Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates by Shugang Li, Zhihao Li, Xiuting Li, Ping Wang, Xiongwei Yu, Qinli Fu, Sihai Gao

“…The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g, α-helix decreased and β-sheet decreased in secondary structure, and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g, enthalpy (ΔH) and denaturation temperature increased (Td) (P < 0.05). By mass spectrometry results of simulated gastric digestion products, it was found that oxidation adversely affected the digestion characteristics of WMP. …”
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Recent Progress in Enzyme Immobilization to Metal–Organic Frameworks to Enhance the CO₂ Conversion Efficiency by Yunhan Cao, Pengyan Yang, Rui Zhao, Fenghuan Wang

“…However, free enzymes are prone to inactivation and denaturation under reaction conditions, which limit their practical applications. …”
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Challenges and strategies in the soluble expression of CTA1-(S14P5)4-DD and CTA1-(S21P2)4-DD fusion proteins as candidates for COVID-19 intranasal vaccines. by Simson Tarigan, Gita Sekarmila, Apas, Sumarningsih, Ronald Tarigan, Riyandini Putri, Damai Ria Setyawati

“…Inducing expression with IPTG at the early log phase significantly increased soluble CTA1-(S21P2)4-DD but not CTA1-(S14P5)4-DD. Adding non-denaturing detergents (Nonidet P40, Triton X100, or Tween 20) to the extraction buffer significantly enhanced solubility. …”
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Resin-Dentin Bonding Interface: Mechanisms of Degradation and Strategies for Stabilization of the Hybrid Layer by D. E. Betancourt, P. A. Baldion, J. E. Castellanos

“…Thus, unprotected fibrils are exposed to undergo denaturation and are susceptible to cyclic fatigue rupture after being subjected to repetitive loads during function. …”
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Impact of frequent ARID1A mutations on protein stability provides insights into cancer pathogenesis by Rajen K. Goutam, Gangtong Huang, Exequiel Medina, Feng Ding, William J. Edenfield, Hugo Sanabria

“…By combining computational stability from web server tools, the structural dynamics from replica exchange discrete molecular simulation (rexDMD), and thermal and chemical denaturation experiments, we found that the R1020S mutation severely decreases structural stability, making it the most impactful, while M1022K has minimal effect, and others lie in between. …”
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The Stability Improvement of α-Amylase Enzyme from Aspergillus fumigatus by Immobilization on a Bentonite Matrix by Yandri Yandri, Ezra Rheinsky Tiarsa, Tati Suhartati, Heri Satria, Bambang Irawan, Sutopo Hadi

“…Furthermore, the soluble enzyme was immobilized using 0.1 M phosphate buffer of pH 7.5 on a bentonite matrix, after which it was characterized with the following parameters such as optimum temperature, Michaelis constant (KM), maximum velocity Vmax, thermal inactivation rate constant (ki), half-life (t1/2), and the change of energy due to denaturation (ΔGi). The results showed that the soluble enzyme has an optimum temperature of 55°C, KM of 3.04 mg mL−1 substrate, Vmax of 10.90 μmole mL−1 min−1, ki of 0.0171 min−1, t1/2 of 40.53 min, and ΔGi of 104.47 kJ mole−1, while the immobilized enzyme has an optimum temperature of 70°C, KM of 8.31 mg mL−1 substrate, Vmax of 1.44 μmole mL−1 min−1, ki of 0.0060 min−1, t1/2 of 115.50 min, and ΔGi of 107.37 kJ mole−1. …”
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Mechanical Properties of Different Lithological Rocks: A Case Study of the Coal Measure Strata in the Eastern Margin of Ordos Basin, China by Xiong Jian, Wu Jianjun, Liu Junjie, Li Bing, Liu Xiangjun, Liang Lixi

“…Under the uniaxial compression condition, the peak strain of different lithological rocks is obviously different, and the denaturation characteristics are also obviously different, and the failure modes of rocks are mainly the tensile fracture mode, suggesting that the rock samples have strong brittle characteristics. …”
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Gastroprotective properties of flavonoid-rich extract of Pulicaria odora against ethanol-induced gastric ulcer in mice by Khadidja Boudebbaz, Manel Brouk, Roumaissa Laalem, Nada Zabaiou

“…In vitro anti-inflammatory activity was investigated through the human red blood cell membrane stabilization method and the bovine serum albumin denaturation method. The in vivo anti-inflammatory properties of the crude extract were assessed in mice via a carrageenan-induced acute paw edema model. …”
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Biorefinery—inspired, two-step valorization strategy to manage plant-based recalcitrant organic waste, involving solvent extraction, and fermentation with Bacillus clausii—a proof... by Sreejith Meppoyilam, Ajith Madhavan, Chinchu Bose, Jayasree Pooja, Swetha Suresh, Bipin G. Nair, Sanjay Pal

“…This novel two-step valorization workflow involves the extraction of small molecules using organic solvents, and fermentation of resulting denatured residues (increased biodegradability or decreased recalcitrance) of reduced microbial load. …”
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The Stability Improvement of Aspergillus fumigatus α-Amylase by Immobilization onto Chitin-Bentonite Hybrid by Ezra Rheinsky Tiarsa, Yandri Yandri, Tati Suhartati, Heri Satria, Bambang Irawan, Sutopo Hadi

“…The free and immobilized enzymes were characterized by optimum temperature, Michaelis constant (KM), maximum velocity Vmax, thermal inactivation rate constant (ki), half-life (t1/2), and transformation of free energy because of denaturation (ΔGi). The free enzyme has optimum temperature of 55°C, KM = 3.04 mg mL−1 substrate, Vmax=10.90 μmolemL−1min−1, ki = 0.0171 min−1, t1/2 = 40.53 min, and ΔGi = 104.47 kJ mole−1. …”
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Study on the regulation of tea polyphenols on the structure and gel properties of myofibrillar protein from Neosalanx taihuensis by Xiangxiang Ni, Mengting Li, Zhiwei Huang, Yinyin Wei, Chaoyi Duan, Ruixi Li, Yajie Fang, Xiu Wang, Mingfeng Xu, Rongrong Yu

“…However, myofibrillar proteins (MPs), as the major component of muscle, are prone to oxidative denaturation. Tea polyphenols (TPs), as natural antioxidants, have broad applications in the area of aquatic product manufacturing. …”
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Development of a Cost-Effective Plasma Thawer: A Potential Game-Changer in Blood Transfusion Technology by Shamrez Shahid, Muhammad Danish Mujib Mujib, Ahmad Zahid Rao, Muhammad Abul Hasan, Saad Ahmed Qazi

“…The samples were clear of solid and gelatinous particles, indicating no protein denaturation or overheating. A buzzer signaled the completion of each cycle. …”
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Preparation of Cod Gelatin Antifreeze Peptides and Its Application of Frozen-Thawed Surimi by Jin ZHANG, Tao YUAN, Xiaomei SHA, Yuelun XU, Yating KUANG, Zizi HU

“…DcAFPs effectively prevented the reduction of myofibrillar protein content during freeze-thaw cycles, inhibited changes in the structure and composition of myofibrillar proteins, and delayed protein denaturation. In conclusion, DcAFPs exhibited significant cryoprotective effects on surimi that subjected to repeated freeze-thaw cycles and outperformed commercial antifreeze agents.…”
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Impact of Microwave Time on the Structure and Functional Properties of Glycosylated Soy 7S Globulins by Jixin Zhang, Tao Cui, Lan Zhang, Huiqing Xu, Jingguo Xu, Jun Wang

“…It also showed the lowest endogenous fluorescence and surface hydrophobicity, and the highest thermal denaturation temperature (76.8 °C). Additionally, microwaving for ≤90 s led to increased peptide modifications, with carbamylation and deamidation being the most prevalent. …”
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