Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning by P. Janczyk, R. Pieper, V. Urubschurov, K. R. Wendler, W. B. Souffrant

“…Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. …”
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Using Commercial Bio-Functional Fungal Polysaccharides to Construct Emulsion Systems by Associating with SPI by Laixin Dai, Qingfu Wang, Lining Wang, Qinghua Huang, Biao Hu

“…Heat treatment had a limited effect on electrostatically driven complexation but resulted in larger particles through a protein-denaturation-induced increase of hydrophobic interactions. …”
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Association of Xmn I Polymorphism and Hemoglobin E Haplotypes on Postnatal Gamma Globin Gene Expression in Homozygous Hemoglobin E by Supachai Ekwattanakit, Yuwarat Monteerarat, Suchada Riolueang, Kalaya Tachavanich, Vip Viprakasit

“…No association was found on a specific haplotype or Xmn I in these individuals with high Hb F, measured by alkaline denaturation. Conclusion. The cis-regulation of γ globin gene expression might not be apparent under a milder condition with lesser globin imbalance such as homozygous Hb E.…”
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Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates by Shugang Li, Zhihao Li, Xiuting Li, Ping Wang, Xiongwei Yu, Qinli Fu, Sihai Gao

“…The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g, α-helix decreased and β-sheet decreased in secondary structure, and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g, enthalpy (ΔH) and denaturation temperature increased (Td) (P < 0.05). By mass spectrometry results of simulated gastric digestion products, it was found that oxidation adversely affected the digestion characteristics of WMP. …”
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Recent Progress in Enzyme Immobilization to Metal–Organic Frameworks to Enhance the CO₂ Conversion Efficiency by Yunhan Cao, Pengyan Yang, Rui Zhao, Fenghuan Wang

“…However, free enzymes are prone to inactivation and denaturation under reaction conditions, which limit their practical applications. …”
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Resin-Dentin Bonding Interface: Mechanisms of Degradation and Strategies for Stabilization of the Hybrid Layer by D. E. Betancourt, P. A. Baldion, J. E. Castellanos

“…Thus, unprotected fibrils are exposed to undergo denaturation and are susceptible to cyclic fatigue rupture after being subjected to repetitive loads during function. …”
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Impact of frequent ARID1A mutations on protein stability provides insights into cancer pathogenesis by Rajen K. Goutam, Gangtong Huang, Exequiel Medina, Feng Ding, William J. Edenfield, Hugo Sanabria

“…By combining computational stability from web server tools, the structural dynamics from replica exchange discrete molecular simulation (rexDMD), and thermal and chemical denaturation experiments, we found that the R1020S mutation severely decreases structural stability, making it the most impactful, while M1022K has minimal effect, and others lie in between. …”
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The Stability Improvement of α-Amylase Enzyme from Aspergillus fumigatus by Immobilization on a Bentonite Matrix by Yandri Yandri, Ezra Rheinsky Tiarsa, Tati Suhartati, Heri Satria, Bambang Irawan, Sutopo Hadi

“…Furthermore, the soluble enzyme was immobilized using 0.1 M phosphate buffer of pH 7.5 on a bentonite matrix, after which it was characterized with the following parameters such as optimum temperature, Michaelis constant (KM), maximum velocity Vmax, thermal inactivation rate constant (ki), half-life (t1/2), and the change of energy due to denaturation (ΔGi). The results showed that the soluble enzyme has an optimum temperature of 55°C, KM of 3.04 mg mL−1 substrate, Vmax of 10.90 μmole mL−1 min−1, ki of 0.0171 min−1, t1/2 of 40.53 min, and ΔGi of 104.47 kJ mole−1, while the immobilized enzyme has an optimum temperature of 70°C, KM of 8.31 mg mL−1 substrate, Vmax of 1.44 μmole mL−1 min−1, ki of 0.0060 min−1, t1/2 of 115.50 min, and ΔGi of 107.37 kJ mole−1. …”
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Mechanical Properties of Different Lithological Rocks: A Case Study of the Coal Measure Strata in the Eastern Margin of Ordos Basin, China by Xiong Jian, Wu Jianjun, Liu Junjie, Li Bing, Liu Xiangjun, Liang Lixi

“…Under the uniaxial compression condition, the peak strain of different lithological rocks is obviously different, and the denaturation characteristics are also obviously different, and the failure modes of rocks are mainly the tensile fracture mode, suggesting that the rock samples have strong brittle characteristics. …”
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The Stability Improvement of Aspergillus fumigatus α-Amylase by Immobilization onto Chitin-Bentonite Hybrid by Ezra Rheinsky Tiarsa, Yandri Yandri, Tati Suhartati, Heri Satria, Bambang Irawan, Sutopo Hadi

“…The free and immobilized enzymes were characterized by optimum temperature, Michaelis constant (KM), maximum velocity Vmax, thermal inactivation rate constant (ki), half-life (t1/2), and transformation of free energy because of denaturation (ΔGi). The free enzyme has optimum temperature of 55°C, KM = 3.04 mg mL−1 substrate, Vmax=10.90 μmolemL−1min−1, ki = 0.0171 min−1, t1/2 = 40.53 min, and ΔGi = 104.47 kJ mole−1. …”
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Study on the regulation of tea polyphenols on the structure and gel properties of myofibrillar protein from Neosalanx taihuensis by Xiangxiang Ni, Mengting Li, Zhiwei Huang, Yinyin Wei, Chaoyi Duan, Ruixi Li, Yajie Fang, Xiu Wang, Mingfeng Xu, Rongrong Yu

“…However, myofibrillar proteins (MPs), as the major component of muscle, are prone to oxidative denaturation. Tea polyphenols (TPs), as natural antioxidants, have broad applications in the area of aquatic product manufacturing. …”
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Development of a Cost-Effective Plasma Thawer: A Potential Game-Changer in Blood Transfusion Technology by Shamrez Shahid, Muhammad Danish Mujib Mujib, Ahmad Zahid Rao, Muhammad Abul Hasan, Saad Ahmed Qazi

“…The samples were clear of solid and gelatinous particles, indicating no protein denaturation or overheating. A buzzer signaled the completion of each cycle. …”
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Preparation of Cod Gelatin Antifreeze Peptides and Its Application of Frozen-Thawed Surimi by Jin ZHANG, Tao YUAN, Xiaomei SHA, Yuelun XU, Yating KUANG, Zizi HU

“…DcAFPs effectively prevented the reduction of myofibrillar protein content during freeze-thaw cycles, inhibited changes in the structure and composition of myofibrillar proteins, and delayed protein denaturation. In conclusion, DcAFPs exhibited significant cryoprotective effects on surimi that subjected to repeated freeze-thaw cycles and outperformed commercial antifreeze agents.…”
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Impact of Microwave Time on the Structure and Functional Properties of Glycosylated Soy 7S Globulins by Jixin Zhang, Tao Cui, Lan Zhang, Huiqing Xu, Jingguo Xu, Jun Wang

“…It also showed the lowest endogenous fluorescence and surface hydrophobicity, and the highest thermal denaturation temperature (76.8 °C). Additionally, microwaving for ≤90 s led to increased peptide modifications, with carbamylation and deamidation being the most prevalent. …”
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Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process by Claudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas Filho

“…Burns are lesions in which the thermal energy of the causative agent transfers heat to the surface of the body, causing superficial or deep damage to the skin with protein denaturation in cells and biochemical maladjustments, which delay and disrupt the cicatricial process, increasing the chances of functional and aesthetic sequelae. …”
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Effect of HTST Thermal Treatments on End-Use Quality Characteristics of Goat Milk by B. D. Rohitha Prasantha, K. M. S. Wimalasiri

“…Total protein (TP%) and nonprotein nitrogen contents were stable IAP but TP reduced significantly (P<0.05) at two weeks storage. The whey protein denaturation increased with pasteurization treatments and storage time. …”
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INVESTIGATING THE BIOCHEMICAL PROFILE AND NUTRITIONAL COMPOSITION OF VERNONIA AMYGDALINA LEAVES: A STUDY ON ANTIOXIDANT PROPERTIES, PHYTOCHEMICAL COMPOSITION, ENZYME INHIBITION, AN... by Olugbenga D. Oloruntola, Fehintoluwa S. Oladebeye, Samuel A. Adeyeye, Simeon O. Ayodele, Ojurereoluwa A. Ayodele, Olufemi E. Adeniji

“…Moreover, the study unveiled the extract’s inhibitory effects on key enzymes and proteins involved in metabolic processes, with notable rates of 29.34% for anti-glucosidase activity, 43.28% for alpha-amylase inhibition, 34.44% for albumin denaturation inhibition, and 57.04% for anti-proteinase activity. …”
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Age and diet affect self-resilience of intestinal microbiome in mice by Chenyi Shao, Shenmin Chen, Huan Yang, Mufan Li, Yinhui Liu, Shu Wen, Jing Xiao, Li Tang

“…In addition, 16S rRNA sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis were performed to identify the bacterial taxa and microbiome structure, respectively.ResultsThe diversity of the gut microbiota in infant mice was recovered by the sixth week, while relative abundance of Ruminococcaceae_UCG_014 was low and did not return to normal levels. …”
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Composition and Significance of Bacterial Microbiota and Volatile Organic Compounds of Swiss-Dutch-Type Cheese as Determined by PCR-DGGE and HS-GC by Beata Nalepa, Magdalena Anna Olszewska, Lidia Hanna Markiewicz, Marek Aljewicz

“…Bacterial diversity and VOCs (acetaldehyde; ketones: acetone, diacetyl, acetoin; alcohols: methanol, ethanol; esters: ethyl acetate, ethyl propionate, ethyl butyrate; fatty acids: acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, isocaproic acid, caproic acid, heptanoic acid) were determined by polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE), and headspace gas chromatography (HS-GC), respectively. …”
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Changes of Quality Characteristics before and after Embrittlement of Oreochromis niloticus×O. aureus by Mei CHEN, Jingkai QIN, Ziyi YANG, Zhongyi QIN, Jie ZHOU, Jiequn JIANG, Xiaoling LIU

“…The freezing point of CT was −0.81℃, lower than that of NT was −0.73 ℃, and the enthalpy of protein denaturation was 21.48% higher than that of NT. The muscle fiber structure of CT was more compact and dense, and the hardness, stickiness and mastication of CT were significantly higher than those of NT (P<0.05). …”
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