Synthesizing hybrid copper phosphate (Cu3(PO4)2) nanoflowers using Cu+2 and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation... by Cagri Caglar Sinmez, Fatih Doğan Koca

“…The catalytic and antimicrobial properties of hNFs were mentioned by the Fenton reaction. The cytotoxicity of Cu@SSS hNFs on the lung carcinoma (A549) cell line was shown to be concentration-dependent by the MTT test assay (IC50: 56.4 µg/ml). …”
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Phytochemical analysis and biological activities of solvent extracts and silver nanoparticles obtained from Woodwardia unigemmata (Makino) Nakai. by Syed Ahsan Shah, Alia Gul, Ghulam Mujtaba Shah, Maha Saeed Ibrahim Wizrah, Awais Khalid, Mamoona Munir, Zainab Maqbool, Arusa Aftab, Mazen R Alrahili, Ayesha Siddiqua, M Yasmin Begum

“…The ethanolic extracts (500 μg, 1000 μg, 2000 μg) of the W. unigemmata were found to have cytotoxicity against both gastric cancer (AGS and SGC7901) and normal cell lines (GES-1); a significant cellular proliferation arrest was observed. …”
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Study on the Mechanism of Black Phosphorus Nanosheets Loading Sr2+ Used in Photothermal Antibacterial Treatment by Ma G, Li B, Diao J, Zhang Y, Zhang B, Wu D, Gui H, Zhong J, Zhu H, Zhang D

“…The cytocompatibility of BP-Sr was evaluated by CCK8 assay and live/dead staining which showed no significant cytotoxicity with a concentration not exceed 50 μg/mL. …”
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An indole alkaloid from a tribal folklore inhibits immediate early event in HSV-2 infected cells with therapeutic efficacy in vaginally infected mice. by Paromita Bag, Durbadal Ojha, Hemanta Mukherjee, Umesh Chandra Halder, Supriya Mondal, Nidhi S Chandra, Suman Nandi, Ashoke Sharon, Mamta Chawla Sarkar, Sekhar Chakrabarti, Debprasad Chattopadhyay

“…Based on ethnomedicinal use we have isolated a compound 7-methoxy-1-methyl-4,9-dihydro-3H-pyrido[3,4-b]indole (HM) from the traditional herb Ophiorrhiza nicobarica Balkr, and evaluated its efficacy on isolates of HSV-2 in vitro and in vivo. The cytotoxicity (CC50), effective concentrations (EC50) and the mode of action of HM was determined by MTT, plaque reduction, time-of-addition, immunofluorescence (IFA), Western blot, qRT-PCR, EMSA, supershift and co-immunoprecipitation assays; while the in vivo toxicity and efficacy was evaluated in BALB/c mice. …”
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Synthesis and Characterization of Green Zinc Oxide Nanoparticles with Antiproliferative Effects through Apoptosis Induction and MicroRNA Modulation in Breast Cancer Cells by Amir Hossein Aalami, Mohammad Mesgari, Amirhossein Sahebkar

“…Cellular studies were conducted using ZnO-NPs before miRNA investigations including MTT cytotoxicity test against MCF7, MDA-MB-231, and HFF cell lines. …”
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In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection mode... by Pompi Das, Debajit Mahanta, Sharmila Giogi, Tarh Kaha, Ngurang Nisha, Sanjeeb Kalita

“…Controlled release studies of amoxicillin-loaded composites demonstrated sustained drug release over 72 hours, achieving a cumulative release of 81.6 % in BFS-SNP-AMOX. Cytotoxicity assessment on L929 fibroblasts confirmed the biocompatibility of the composites, with cell viabilities exceeding 90 %. …”
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CoronaVac-induced antibodies that facilitate Fc-mediated neutrophil phagocytosis track with COVID-19 disease resolution by Chuang Li, Jie Yu, Rahma Issa, Lili Wang, Mingzhe Ning, Shengxia Yin, Jie Li, Chao Wu, Yuxin Chen

“…In addition to neutralization, antibodies can mediate various Fc-dependent effector functions, including antibody-dependent cellular phagocytosis (ADCP), antibody-dependent neutrophil phagocytosis (ADNP) and antibody-dependent cellular cytotoxicity (ADCC). However, the specific role of each Fc-mediated effector function in contributing to COVID-19 disease attenuation in human remains unclear. …”
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Green biosynthetic silver nanoparticles from Ageratum conyzoides as multifunctional hemostatic agents: Combining hemostasis, antibacterial, and anti-inflammatory properties for eff... by Yang Li, Yinfeng Tan, Huange Zhao, Shiting Chen, Azadeh Nilghaz, Rong Cao, Songlin Zhou

“…In-vitro coagulation experiments indicate that AC-AgNPs have a strong hemostatic effect, which is related to their size, concentration, and negative charge, and they exhibit low cytotoxicity and hemolysis. Subsequent experiments including scanning electron microscopy, flow cytometry, western blotting, and network pharmacology analysis have revealed that AC-AgNPs can cause platelet activation and aggregation, stimulated kallikrein-kinin system, shorten activated partial thromboplastin and prothrombin time, and increase fibrinogen content. …”
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The transcriptional regulator Lrp activates the expression of genes involved in the biosynthesis of tilimycin and tilivalline enterotoxins in Klebsiella oxytoca by Miguel A. De la Cruz, Hilda A. Valdez-Salazar, Diana Rodríguez-Valverde, Santa Mejia-Ventura, Nayely Robles-Leyva, Tania Siqueiros-Cendón, Quintín Rascón-Cruz, Nancy León-Montes, Jorge Soria-Bustos, Fernando Chimal-Cázares, Roberto Rosales-Reyes, María L. Cedillo, Jorge A. Yañez-Santos, J. Antonio Ibarra, Javier Torres, Jorge A. Girón, James G. Fox, Miguel A. Ares

“…Furthermore, the lack of Lrp significantly diminished the cytotoxicity of K. oxytoca on HeLa cells due to reduced production of TM and TV. …”
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Key considerations based on pharmacokinetic/pharmacodynamic in the design of antibody-drug conjugates by Yangyang Gao, Yuwei Xia, Yixin Chen, Shiqi Zhou, Yingying Fang, Jieru Yu, Leyin Zhang, Leyin Zhang, Leitao Sun, Leitao Sun, Leitao Sun

“…BackgroundAntibody-drug conjugate (ADC) is an anticancer drug that links toxins to specifically targeted antibodies via linkers, offering the advantages of high target specificity and high cytotoxicity. However, complexity of its structural composition poses a greater difficulty for drug design studies.ObjectivesPharmacokinetic/pharmacodynamic (PK/PD) based consideration of ADCs has increasingly become a hot research topic for optimal drug design in recent years, providing possible ideas for obtaining ADCs with desirable properties.MethodsFrom the assessment of the ADC action process based on PK/PD, we introduce the main research strategies of ADCs. …”
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ALDH2 Overexpression Alleviates High Glucose-Induced Cardiotoxicity by Inhibiting NLRP3 Inflammasome Activation by Ruiping Cao, Dian Fang, Jiahui Wang, Ying Yu, Hongwei Ye, Pinfang Kang, Zhenghong Li, Hongju Wang, Qin Gao

“…The H9C2 cardiac cells were exposed to 35 mM glucose for 24 h to induce cytotoxicity. Mitochondrial ALDH2 overexpression cardiac cell line was constructed. …”
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The Protective Effect of Mangiferin on Formaldehyde-Induced HT22 Cell Damage and Cognitive Impairment by Fan Chen, Na Wang, Xinyan Tian, Juan Su, Yan Qin, Rongqiao He, Xiaping He

“…The results in murine hippocampal cells (HT22) revealed that co-treatment with MGF significantly decreased FA-induced cytotoxicity and inhibited Tau hyperphosphorylation in a dose-dependent manner. …”
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NeuTox 2.0: A hybrid deep learning architecture for screening potential neurotoxicity of chemicals based on multimodal feature fusion by Xudi Pang, Xuejun He, Ying Yang, Ling Wang, Yuzhen Sun, Huiming Cao, Yong Liang

“…Four datasets were used to profile neurotoxicity; these were related to blood–brain barrier permeability, neuronal cytotoxicity, microelectrode array–based neural activity, and mammalian neurotoxicity. …”
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Unleashing the potential of a low CpG Passer transposon for superior CAR-T cell therapy by Jianyao Zeng, Yan Sun, Yan Sun, Yuan Fang, Xiaodie Wang, Qian Huang, Pingjing Zhang, Meiqi Shao, Pei Wang, Jingbo Cheng, Meng Di, Tao Liu, Qijun Qian, Qijun Qian, Qijun Qian

“…In vitro experiments demonstrated that LCG CAR-T cells prepared using this new transposon exhibited stronger cytotoxicity. In animal models, LCG CAR-T cells significantly inhibited tumor growth and increased the populations of CD4+CAR-T cells and tumor-infiltrating lymphocytes. …”
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Construction of a novel radioresistance-related signature for prediction of prognosis, immune microenvironment and anti-tumour drug sensitivity in non-small cell lung cancer by Yanliang Chen, Chan Zhou, Xiaoqiao Zhang, Min Chen, Meifang Wang, Lisha Zhang, Yanhui Chen, Litao Huang, Junjun Sun, Dandan Wang, Yong Chen

“…In vitro experiments indicated that overexpression of LBH enhanced the radiosensitivity of A549 cells, and knockdown LBH reversed the cytotoxicity induced by X-rays.Conclusion Our study developed an eight-gene risk score model with potential clinical value that can be adopted for choice of drug treatment and prognostic prediction. …”
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Isoxazole-based molecules restore NK cell immune surveillance in hepatocarcinogenesis by targeting TM4SF5 and SLAMF7 linkage by Ji Eon Kim, Hyun Su Kim, Wonsik Kim, Eun Hae Lee, Soyeon Kim, Taewoo Kim, Eun-Ae Shin, Kyung-hee Pyo, Haesong Lee, Seo Hee Jin, Jae-Ho Lee, Soo-Min Byeon, Dong Joo Kim, Jinwook Jeong, Jeongwon Lee, Minjae Ohn, Hyojung Lee, Su Jong Yu, Dongyun Shin, Semi Kim, Jun Yeob Yoo, Seung-Chul Lee, Young-Ger Suh, Jung Weon Lee

“…TM4SF5 expressed by hepatocytes reduced NK cell cytotoxicity by downregulating stimulatory ligands/receptors, including signaling lymphocytic activation molecule family member 7 (SLAMF7). …”
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Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer by Markus Hoffmann, Markus Hoffmann, Susanne Hille, Susanne Hille, Susanne Hille, Asita Fazel, Martin Laudien, Martin Laudien, Susanne Wiegand, Susanne Wiegand, Martin Müller, Oliver J. Müller, Oliver J. Müller, Oliver J. Müller, Elgar Susanne Quabius, Elgar Susanne Quabius

“…Protein isolation, SLPI- and AnxA2-quantification, LDH cytotoxicity assessment, HPV16-PsV-uptake measurement, mRNA-isolation, cDNA-synthesis and RT-qPCR were performed.ResultsIn vitro transfection experiments with HPV16 pseudovirions (PsVs) showed that PsVs entered cells significantly better when SLPI was downregulated and significantly less when AnxA2 was downregulated. …”
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Preclinical evaluation of avutometinib and defactinib in high‐grade endometrioid endometrial cancer by Tobias Max Philipp Hartwich, Miranda Mansolf, Cem Demirkiran, Michelle Greenman, Stefania Bellone, Blair McNamara, Shuvro P. Nandi, Ludmil B. Alexandrov, Yang Yang‐Hartwich, Silvia Coma, Jonathan Pachter, Alessandro D. Santin

“…The in vitro activity of avutometinib and defactinib as single agents and in combination was evaluated using cell viability, cell cycle, and cytotoxicity assays. Mechanistic studies were performed using Western blot assays while in vivo experiments were completed in UTE10 engrafted mice treated with either vehicle, avutometinib, VS‐4718, or their combination through oral gavage. …”
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Tumor CEMIP drives immune evasion of colorectal cancer via MHC-I internalization and degradation by Ying Zhu, Jiao Li, Tao Zhang, Lei Zhao, Min Jin, Jinghua Ren, Jing Tang, Haihong Wang, Zhenyu Lin, Qing Liang, Ruiqi Li, Biying Zhang, Qingling Hua, Guojie Xu, Jiayuan Chen, Lanqing Wang, Dejun Zhang, Dandan Yu

“…In addition, the effect of CEMIP on tumor growth and the antitumor efficacy of targeting CEMIP in combination with ICB therapy were evaluated in murine models of colorectal cancer.Results We reported that CEMIP specifically downregulated the expression of MHC-I on the surface of murine and human colon cancer cells, hindering the cytotoxicity of CD8+ T cells. We also demonstrated that CEMIP restricted CD8+ T-cell antitumor activities both in vitro and in vivo due to impaired MHC-I-mediated antigen presentation. …”
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Effects of Compressive and Tensile Strain on Macrophages during Simulated Orthodontic Tooth Movement by Agnes Schröder, Paul Käppler, Ute Nazet, Jonathan Jantsch, Peter Proff, Fabian Cieplik, James Deschner, Christian Kirschneck

“…We determined cell number, cytotoxicity, and gene/protein expression of Vegf-a/VEGF-A (macrophage-mediated angiogenesis), Mmp-8/9 (extracellular matrix reorganization), and Cox-2/PG-E2, Il-6/IL-6, and Tnf-α/TNF-α (proinflammatory mediators) by RT-qPCR and ELISA. …”
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