Choriocarcinoma Syndrome as an Initial Presentation of Testicular Cancer by Carlos Eduardo Salazar-Mejía, María Elena García-Gutiérrez, María Inés Contreras-Salcido, Carlos Javier Rodríguez-Álvarez, Blanca Otilia Wimer-Castillo, Jackeline Grace Lara-Campos, Edio Llerena-Hernández, José Luis González-Vela, David Hernández-Barajas

“…Due to the severity of the presentation, hemodynamic monitoring, ideally in an intensive care unit, is essential as well as timely administration of cytotoxic treatment.…”
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A bispecific antibody-drug conjugate targeting pCAD and CDH17 has antitumor activity and improved tumor-specificity by Alyssa Synan, Nila C. Wu, Roberto Velazquez, Thomas Gesner, Claude Logel, Kathrin Mueller, Andrew Green, Patrizia Barzaghi-Rinaudo, Quincey Simmons, Samuele Mercan, Xingyi Shi, Xun Li, Vivek Sagar, Joshua Korn, Margaret McLaughlin, William R. Tschantz, Dominik Hainzl, Anthony Malamas, Thomas Huber, Regis Cebe, Kathleen T. Xie, Joseph A. D’Alessio

“…Based on in vitro binding and inhibition of cell proliferation results, we selected a lead bispecific antibody to link to the cytotoxic payload monomethyl auristatin E (MMAE) to generate a pCAD x CDH17 bispecific MMAE ADC. …”
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Phytosomes Loaded with Mastoparan-M Represent a Novel Strategy for Breast Cancer Treatment by Zhao H, Bao S, Chen S, Yang Q, Lou K, Gai Y, Lin J, Liu C, Liu H, Zhang C, Yang R

“…Hairong Zhao,1,2,* Shuangyan Bao,2,* Shuanglong Chen,1,* Qingmo Yang,1,* Kangliang Lou,3 Yating Gai,4 Jinyan Lin,5 Chaojie Liu,2 Heng Liu,2 Chenggui Zhang,2 Ruiqin Yang1 1Department of Breast Surgery, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, People’s Republic of China; 2Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, People’s Republic of China; 3School of Medicine, Xiamen University, Xiamen, People’s Republic of China; 4Xiamen Research Institute of Food and Drug Quality Inspection, Xiamen, People’s Republic of China; 5School of Public Health, Xi’an Jiaotong University, Xi’an, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ruiqin Yang, Department of Breast Surgery, the First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, People’s Republic of China, Email yrqxm0822@163.com Chenggui Zhang, Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, People’s Republic of China, Email chenggui_zcg@163.comPurpose: Mastoparan-M (Mast-M) has cytotoxic effects on various tumor cells in vitro, including liver cancer and colorectal cancer. …”
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Germinal Center B Cells are Uniquely Targeted by Antibody-Suppressor CXCR5+CD8+ T Cells by Jason M. Zimmerer, PhD, Sachi Chaudhari, BS, Kavya Koneru, BS, Jing L. Han, MD, PhD, Mahmoud Abdel-Rasoul, MA, Hope Uwase, MA, Tai Yi, MD, Christopher K. Breuer, MD, Ginny L. Bumgardner, MD, PhD

“…Alloprimed antibody-suppressor CXCR5+CD8+ T cells (CD8+ TAb-supp cells) downregulate alloantibody production, mediate cytotoxicity of IgG+ B cells, and prolong allograft survival. …”
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Bisphenol Analogs Downregulate the Self-Renewal Potential of Spermatogonial Stem Cells by Seo-Hee Kim, Seung Hee Shin, Seok-Man Kim, Sang-Eun Jung, Beom-Jin Shin, Jin Seop Ahn, Kyoung Taek Lim, Dong-Hwan Kim, Kichoon Lee, Buom-Yong Ryu

“…Conclusions: Our findings indicate that the major BPA substitutes, BPF and BPS, have significant cytotoxic effects on SSCs, similar to BPA. These effects may lead to a reduction in the functional self-renewal stem cell population and potential impacts on male fertility.…”

WT1 Peptide Cancer Vaccine for Patients with Hematopoietic Malignancies and Solid Cancers by Yoshihiro Oka, Akihiro Tsuboi, Olga A. Elisseeva, Hiroko Nakajima, Fumihiro Fujiki, Manabu Kawakami, Toshiaki Shirakata, Sumiyuki Nishida, Naoki Hosen, Yusuke Oji, Ichiro Kawase, Haruo Sugiyama

“…Wild-type Wilms' tumor gene WT1 is expressed at a high level in hematopoietic malignancies including acute leukemia, chronic myelogenous leukemia, and myelodysplastic syndromes, as well as in various kinds of solid cancers. Human cytotoxic T lymphocytes (CTLs), which could specifically lyse WT1-expressing tumor cells with HLA class I restriction, were generated in vitro. …”
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Exhaustion of CD8pos central memory regulatory T cell differentiation is involved in renal allograft rejection by Florian Kälble, Jonas Leonhard, Jonas Leonhard, Martin Zeier, Oliver Zivanovic, Matthias Schaier, Andrea Steinborn

“…BackgroundThe role of regulatory CD8pos T cells (CD8pos Tregs) and cytotoxic CD8pos responder T cells (CD8pos Tresps) in maintaining stable graft function in kidney transplant recipients (KTR) remains largely unclear. …”
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Monocytes serve as Shiga toxin carriers during the development of hemolytic uremic syndrome by Xinlei Sun, Shuang Qu, Fenglian Zhou, Fujie Shi, Yunfei Wu, Lin Gu, Minghui Liu, Zhen Bian, Lei Shi, Zhihong Liu, Yuan Liu, Ke Zen

“…Abstract Shiga toxin (Stx)-induced hemolytic uremic syndrome (HUS) poses a life-threatening complication for which a definitive treatment remains elusive. To exert its cytotoxic effect on renal cells, Stx must be delivered from the infected intestines to the kidney. …”
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Synthesis, X-Ray Crystal Structures, Biological Evaluation, and Molecular Docking Studies of a Series of Barbiturate Derivatives by Assem Barakat, Hazem A. Ghabbour, Abdullah Mohammed Al-Majid, Qurat-ul-ain, Rehan Imad, Kulsoom Javaid, Nimra Naveed Shaikh, Sammer Yousuf, M. Iqbal Choudhary, Abdul Wadood

“…All barbiturates derivatives (4a–4x, 4z, and 5a–5m) were found to be noncytotoxic against human prostate (PC-3), Henrietta Lacks cervical (HeLa) and Michigan Cancer Foundation-7 breast (MCF-7) cancer cell lines, and 3T3 normal fibroblast cell line, except 4y which was cytotoxic against all the cell lines.…”
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Pharmacological Vitamin C-induced high H2O2 generation mediates apoptotic cell death by caspase 3/7 activation in breast cancer tumor spheroids by Ali Mussa, Mahasin Hamid, Khalid Hajissa, Ahmad Hafiz Murtadha, Mohammad A. I. Al-Hatamleh, Noor Fatmawati Mokhtar, Vuk Uskoković, Magdalena Plebanski, Rohimah Mohamud, Rosline Hassan

“…However, MDA-MB-231 spheroids exhibited a more severe cytotoxic response. Conclusions This study reveals that Vit-C induces oxidative stress-mediated cell death in both non-aggressive and aggressive BC spheroids. …”
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Assessments of prostate cancer cell functions highlight differences between a pan‐PI3K/mTOR inhibitor, gedatolisib, and single‐node inhibitors of the PI3K/AKT/mTOR pathway by Adrish Sen, Salmaan Khan, Stefano Rossetti, Aaron Broege, Ian MacNeil, Ann DeLaForest, Jhomary Molden, Laura Davis, Charles Iversrud, Megan Seibel, Ross Kopher, Stephen Schulz, Lance Laing

“…Gedatolisib induced anti‐proliferative and cytotoxic effects with greater potency and efficacy relative to the other PAM inhibitors, independent of PTEN/PIK3CA status. …”
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Breast Cancer Conditioned Media Modulate the Expression of Immune Checkpoints and Adhesion Molecules in Endothelial Cells by İpek Türkdönmez, Emel Şahin, Mehmet Şahin

“…Materials and Methods: In this study, the expression levels of some immune checkpoints, including lymphocyte-activation-protein 3 (LAG-3), V-domain Ig suppressor of T-cell activation (VISTA), Indoleamine-2,3-dioxygenase (IDO), IDO-2, programmed cell death 1 ligand (PD-L1), PD-L2, Cytotoxic T-lymphocyte associated protein 4, (CTLA-4), and some adhesion molecules, including E-cadherin, VE-cadherin, E-selectin, platelet EC adhesion molecule-1 (PECAM-1), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), were measured using quantitative real-time polymerase chain reaction in HUVEC ECs preincubated with the conditioned media (CM) collected from normal (CRL-4010) and carcinoma cells (CRL-2329 and MDA-MB-231). …”
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PD‐1+CD8+ T Cell‐Mediated Hepatocyte Pyroptosis Promotes Progression of Murine Autoimmune Liver Disease by Jie Long, Si‐Yu Yang, Zhen‐Hua Bian, Hao‐Xian Zhu, Min Ma, Xiao‐Qing Wang, Liang Li, Weici Zhang, Ying Han, M. Eric Gershwin, Zhe‐Xiong Lian, Zhi‐Bin Zhao

“…These results provide a novel explanation for the cytotoxic activity of pathogenic liver PD‐1+CD8+ T cells in autoimmune liver diseases and offer potential therapeutic targets.…”
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Targeting TCMR-associated cytokine genes for drug screening identifies PPARγ agonists as novel immunomodulatory agents in transplantation by Lu Hu, Lu Hu, Xiaohan Zhang, Xiaohan Zhang, Weiqi Zhang, Weiqi Zhang, Shuai Jin, Shuai Jin, Jie Zhao, Jianming Zheng, Wenli Song, Zhongyang Shen, Zhongyang Shen, Zhongyang Shen

“…Experimental validation showed that the PPARγ agonist rosiglitazone significantly suppressed T cell activation and reduced Th1 differentiation in vitro without cytotoxic effects. The combination of rosiglitazone and rapamycin significantly prolonged graft survival.ConclusionsThis study defined a novel TCMR-associated cytokine gene set that effectively predicts TCMR and identified PPARγ agonists, which prevent TCMR and improve graft survival when combined with rapamycin.…”
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Targeting Siglec-E facilitates tumor vaccine-induced antitumor immunity in renal carcinoma by Yuchen Zhang, Junnian Zheng, Li Sun, Guangya Zhao, Yingxiang Shao, Bowen Lu, Jiage Ding, Yanyan Zheng, Renjin Chen, Dafei Chai, Jiawei Wang, Zichun Zhang, Xiaohui Xie

“…Immune responses induced by Ad-Siglec-E/CAIX were assessed in vitro and in vivo using flow cytometry, immunohistochemistry, ELISA, histological analysis, cell proliferation, enzyme-linked immunosorbent spot, cytotoxic T lymphocytes (CTL) killing, and cell depletion assays.Results Ad-Siglec-E/CAIX vaccine induced the increase of tumor-infiltrated immune cells, and significantly suppressed the subcutaneous tumor growth of renal carcinoma. …”
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Anti-inflammatory effects of Andrographis paniculata (Fah Talai Jone) via TNFα-JNK pathway and bioactive compound identification by Praphat Manuelo Ruengthanoo, Areeya Changthong, Pranee Sriraj, Jatuporn Prathumtet, Nutchapon Laikaew, Ratchadawan Aukkanimart

“…Study Design: Experimental research was conducted to assess the antioxidant, cytotoxic and anti-inflammatory properties of both ethanol (APE) and aqueous (APA) extracts of A. paniculata using in vitro methods. …”
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Impact of long-term cyanotoxin exposure on cattle: Biochemical, histological, and oxidative stress assessment by Mounira Bensalem, Amina Amrani, Hadjer Zaidi, Fateh Sedrati, Omar Laouar, Zhi Wang, Hichem Nasri

“…These findings reflect the cumulative cytotoxic effects of MC exposure. Non-exposed cattle showed no such changes. …”
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Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function by Jianbo Wu, Jian Gu, Shun Zhou, Hao Lu, Yunjie Lu, Ling Lu, Xuehao Wang

“…Transfer of splenocytes isolated from B6 mice into normal B6D2F1 mice induces acute graft-versus-host disease (aGVHD), resulting in the expansion of donor cytotoxic T lymphocytes that eliminate recipient B cells. …”
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Insights into bioactivity guided chemical profiling of Ziziphus jujuba Mill. fruits wild-growing in Montenegro by Tatjana Perović, Biljana Lazović, Mirjana Adakalić, Ana Džamić, Lazar Žarković, Uroš Gašić, Marina Kostić, Jovana Petrović, Dejan Stojković, Ana Ćirić

“…It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively explored. …”
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Lactoferrin Modulates Radiation Response Under Hypoxic Conditions, Possibly Through the Regulation of ROS Production in a Cell Type-Specific Manner by Daitoku Murakami, Takahiro Fukazawa, Michihito Kyo, Mutsumi Miyauchi, Shigehiro Ono, Tomonao Aikawa, Nobuyuki Hirohashi, Keiji Tanimoto

“…High-dose LF treatment showed cytotoxic activity in a variety of cells, including both non-cancer and cancer cells. …”
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