Fermented sugarcane juice-derived probiotic Levilactobacillus brevis RAMULAB54 enhances lipid metabolism and glucose homeostasis through PPAR-γ activation by V. B. Chandana Kumari, Ramith Ramu, Sujay S. Huligere, Shashank M. Patil, Shivasharanappa Nayakvadi, Sharath Bijoor, Uma Venkateswaran Manjappara, Mohammad Z. Ahmed, Ling Shing Wong

“…This study is a novel approach that explores the antihyperglycemic and antihyperlipidemic effects of Levilactobacillus brevis RAMULAB54 (LB13243), isolated from fermented sugarcane juice. LB13243 was cultured for SEM imaging, and its supernatant (LBR54) was analyzed. …”
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Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells at Various Passages on Acute Liver Failure in Rats by Yongting Zhang, Yuwen Li, Wenting Li, Jie Cai, Ming Yue, Longfeng Jiang, Ruirui Xu, Lili Zhang, Jun Li, Chuanlong Zhu

“…However, data on the therapeutic potential of culture-expanded MSCs are lacking. We examined the therapeutic potential of passage five (P5) and ten (P10) human umbilical cord- (hUC-) MSCs via their transplantation into Sprague-Dawley (SD) rats with D-galactosamine (D-GalN) and LPS-induced acute liver failure (ALF). …”
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Microenvironmental G protein‐coupled estrogen receptor‐mediated glutamine metabolic coupling between cancer‐associated fibroblasts and triple‐negative breast cancer cells governs t... by Chongwu He, Meixi Peng, Xiaoqiang Zeng, Hanzhi Dong, Zhengkui Sun, Jiawei Xu, Manran Liu, Liyan Liu, Yanxiao Huang, Zhiqiang Peng, Yu‐An Qiu, Chunling Jiang, Bin Xu, Tenghua Yu

“…This study investigates how GPER influences the metabolic interaction between CAFs and TNBC cells, aiming to identify potential therapeutic targets. Methods The co‐culture system is performed to examine the interaction between CAFs and TNBC cells, with a focus on GPER‐mediated glutamine production and release by CAFs and its subsequent uptake and utilization by TNBC cells. …”
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Long non-coding RNA Malat1 modulates CXCR4 expression to regulate the interaction between induced neural stem cells and microglia following closed head injury by Qin Dong, Pengyu Chen, Wenqiao Qiu, Zhijun Yang, Yanteng Li, Yuhui Zhou, Lili Guo, Dan Zou, Ruxiang Xu, Mou Gao

“…Methods We performed bioinformatic analysis to detect the differentially expressed genes in iNSCs after co-cultured with LPS-activated microglia. Subsequently, we predicted the target genes and performed gain- and loss-of-functional studies, dualluciferase reporter, RNA immunoprecipitation, biotin-coupled miRNA pulldown, fluorescence in situ hybridization and cell transplantation assays to further elucidate the mechanism underlying the immunoregulatory effects of iNSCs. …”
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Impact of carbapenem-resistant infections on mortality in mechanically ventilated acute respiratory distress syndrome patients: A comparison with hypoxemia severity – An observatio... by Thejesh Srinivas, Shwethapriya R, Gagana Hanumaiah, Pratibha Todur, Souvik Chaudhuri, Ganesh Paramasivam, Prithvishree Ravindra, Vinutha R. Bhat, Sagar Shanmukhappa Maddani, Shobha U. Kamath, Danavath Nagendra, Vishwas P, Likith Hanumaiah, Pratik Paran Medhi, Prabha Prakash

“…Demographic details, organ dysfunction scores, oxygenation values, ARDS classification based on the Berlin criteria, inflammatory biomarkers, and ICU outcomes were noted from clinical records. Patients’ culture sensitivity reports were reviewed for CRI, and the association of CRI with mortality outcomes was analyzed. …”
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Altered immune surveillance of B and T cells in patients with persistent residual lung abnormalities 12 months after severe COVID-19 by Julio Flores-Gonzalez, Ivette Buendia-Roldan, Fernanda Téllez-Quijada, Carlos Peña-Bates, Lucero A. Ramón-Luing, Armando Castorena-Maldonado, Ramcés Falfán-Valencia, Gloria Pérez-Rubio, Moisés Selman, Leslie Chavez-Galan, Leslie Chávez-Galán

“…Results Compared to the FRG group, PLAG exhibited an increase of unswitched (p = 0.0159) and decreased double-negative activated B-cells (p = 0.0317), systemic IL-10 levels were lower, displayed reduced frequency of total B-cells, and impaired spontaneous IgM (p = 0.0357) and IgG (p = 0.0079) release in culture. Regarding T-cells, PLAG patients showed a reduction in effector memory CD4 + cells (p = 0.0159) and an increase in CD4 + TEMRA cells (p = 0.0079) following in vitro stimulation. …”
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Dual role of ramR mutation in enhancing immune activation and elevating eravacycline resistance in Klebsiella pneumoniae by Wei Yu, Peiyao Jia, Xiaobing Chu, Shengjie Li, Xinmiao Jia, Ying Zhu, Xiaoyu Liu, Yingchun Xu, Qiwen Yang

“…Furthermore, despite lower intracellular bacterial loads, ramR L58P and F165L mutants induce heightened pro‐inflammatory cytokine responses in macrophages and elevate systemic cytokine levels (interleukin [IL]2, IL4, IL6, IL12, interferon‐α, and interferon‐γ) in human blood co‐culture experiments. This study illuminates the critical role of ramR mutations in conferring ERV resistance and enhancing immune responses in K. pneumoniae. …”
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TCR-engaging scaffolds selectively expand antigen-specific T-cells with a favorable phenotype for adoptive cell therapy by Marco Donia, Özcan Met, Julie Westerlin Kjeldsen, Sine Reker Hadrup, Inge Marie Svane, Arianna Draghi, Anders Handrup Kverneland, Christina Heeke, Siri Amanda Tvingsholm, Marcus Svensson Frej, Vibeke Mindahl Rafa, Ulla Kring Hansen, Maria Ormhøj, Alexander Tyron, Agnete W P Jensen, Mohammad Kadivar, Amalie Kai Bentzen, Kamilla K Munk, Gitte N Aasbjerg, Jeppe S H Ternander, Tripti Tamhane, Christian Schmess, Samuel A. Funt, Søren Nyboe Jakobsen

“…Here, in this study, we present a novel technology for ex vivo expansion of antigen-specific T-cells; artificial antigen-presenting scaffolds (Ag-scaffolds) consisting of a dextran-polysaccharide backbone, decorated with combinations of peptide-Major Histocompatibility Complex (pMHC), cytokines and co-stimulatory molecules, enabling coordinated stimulation of antigen-specific T-cells.Methods The capacity of Ag-scaffolds to expand antigen-specific T-cells was explored in ex vivo cultures with peripheral blood mononuclear cells from healthy donors and patients with metastatic melanoma. …”
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Effects of Different Light Spectra on Oxidative Stress and Nutritional Quality of the Fish <i>Plectropomus leopardus</i> by Wensheng Li, Zheng Zhang, Baoliang Liu, Yingying Fang, Shuquan Cao, Wenyang Li, Yan Sun, Chengbin He, Chuanxin Zhang, Fan Fei

“…The fish (100 g ± 0.45 g [wet weight]) were cultures in five different light spectra (full-spectrum (400–800 nm), blue (450 nm), green (530 nm), red (630 nm), and dark) for 60 days. …”
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Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study by Saray Fernández-Hernández, Javier Gil, Daniel Robles-Cantero, Esteban Pérez-Pevida, Mariano Herrero-Climent, Aritza Brizuela-Velasco

“…The different surfaces were cultured with human osteoblastic cells; cell adhesion was determined at 3 and 14 days, and the degree of mineralization was determined at 14 days via alkaline phosphatase levels. …”
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Antifungal activity and mechanism of Phoebe bournei wood essential oil against two dermatophytes by Yan Yang, Qinglin Sun, Yuting Zhang, Junhua Huang, Wenjun Ma, Qi Yang, Zaikang Tong, Junhong Zhang

“…Yang wood essential oil (PWEO) has been found to have excellent antifungal activity, but its mechanism remains unclear.MethodologyDetermination of minimum inhibitory concentration (MIC) of PWEO on two dermatophytes (Microsporum gypseum and Epidermophyton floccosum) by broth microdilution method. Culture plates containing PWEO in vitro tested PWEO inhibition effect of mycelial growth of dermatophytes and the effect of PWEO on hyphal structure was observed by microscopy; the changes of cell membrane permeability and the degree of lipid peroxidation were reflected by measuring cell physiological indexes. …”
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PU.1 alleviates the inhibitory effects of cigarette smoke on endothelial progenitor cell function and lung-homing through Wnt/β-catenin and CXCL12/CXCR4 pathways by Xue He⁺, Yanan Cui⁺, Tiao Li, Lijuan Luo, Zihang Zeng, Yiming Ma, Yan Chen

“…The angiogenic ability of BM-derived EPCs cultured in vitro was examined by tube formation assay. …”
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Pseudovibriamides from Pseudovibrio marine sponge bacteria promote flagellar motility via transcriptional modulation by Yitao Dai, Vitor Lourenzon, Laura P. Ióca, Dua Al-Smadi, Lydia Arnold, Ian McIntire, Roberto G. S. Berlinck, Alessandra S. Eustáquio

“…We have previously identified pseudovibriamides A and B, produced in culture by Pseudovibrio brasiliensis Ab134, and shown that pseudovibriamide A promotes flagellar motility. …”
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NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice by Jiamin Zhao, Shuangyi Zhang, Zhiguo Gong, Wei Mao, Wenhui Bao, Qianru Li, Yunjie Bai, Feifei Gao, Shuang Feng

“…Furthermore, using co-culture systems, we found that NLRP3 mediated the interaction between macrophages and myofibroblasts. …”
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Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibiti... by Hui Luo, Jianhua Chen, Cao Li, Tian Wu, Siyue Yin, Guangping Yang, Yipin Wang, Zhihan Guo, Saifei Hu, Yanni He, Yingnan Wang, Yao Chen, Youqiang Su, Congxiu Miao, Yun Qian, Ruizhi Feng

“…More importantly, selective inhibition of HDAC6 by culturing TUBB8‐D417N oocytes with Tubacin or Tubastatin A showed morphologically normal spindle and drastically recovered polar‐body extrusion rate. …”
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Designing the alignment model of petrochemical projects with the country's industrial strategies in the Ministry of Petroleum by Abdolrasoul Shojaeian, ghanbar abbaspour esfadan, Changiz Valmohammadi, Aboutorab Alirezaei

“…The concept of strategic alignment originates from a set of experimental and conceptual measures in organizational texts, the main purpose of which is organizational performance and thus the fit between factors such as strategy, structure, technology, culture and environment (Sadigov, 2022). Strategic alignment has been defined as the extent to which IS strategy supports business strategies and is supported by them (Abdulwase et al, 2020). …”
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Designing a model of effective marketing capabilities based on communication by Yoones Banisaeed, Ghanbar Amirnejad, Morteza mousakhani, Leila Andervazh

“…Finally, a strong innovation culture is an important foundation for creating an environment that supports sustainable product innovation. …”
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Genetic profiling of extended-spectrum β-Lactamase and carbapenemase-producing Escherichia coli O157:H7 from clinical samples among diarrheal patients in Shashemene, Ethiopia by Shimelis Teshome Ayalneh, Biruk Yeshitela Beshah, Yeonji Jeon, Ashenafi Alemu Wami, Seifegebriel Teshome, Solomon Gebreselassie, Se Eun Park, Mekonnen Teferi, Woldaregay Erku Abegaz

“…Methods A total of twenty-nine bacterial isolates obtained from diarrheal patients were subjected to conventional culture and phenotypic (Kirby Bauer disc diffusion method) testing for antimicrobial resistance. …”
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Addressing racial and ethnic disparities in premature exits from permanent supportive housing among residents with substance use disorders by Talia J. Panadero, Sonya Gabrielian, Marissa J. Seamans, Lillian Gelberg, Jack Tsai, Taylor Harris

“…Conclusions These findings suggest relationships between SUDs and negative PSH exits differ between racial/ethnic groups and suggest there may be value in culturally specific tailoring and implementation of SUD services for these subgroups.…”
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hUC-MSC preserves erectile function by restoring mitochondrial mass of penile smooth muscle cells in a rat model of cavernous nerve injury via SIRT1/PGC-1a/TFAM signaling by Mengbo Yang, Xinda Chen, Ming Zhang, Xiaolin Zhang, Dongdong Xiao, Huiming Xu, Mujun Lu

“…In vitro, CCSMCs underwent hypoxia and were then co-cultured with ADSC or hUC-MSC using a transwell system. …”
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