TYRO3 blockade enhances anti-PD-1 therapy response by modulating expression of CCN1 in tumor microenvironment by Miso Park, Da-Sol Kuen, Keon Wook Kang, Yeonseok Chung, Jaewoo Park, Munkyung Choi, Yeonji Kim, Eun Chae Roh, Yong June Choi, Yoon Gyoon Kim, Sung Yun Cho

“…Flow cytometry was used for the quantification of cell populations and immunophenotyping of macrophages and T cells. Co-cultures of macrophages and T cells were performed to verify the role of CCN1 in the tumors.Results TYRO3 blockade boosts antitumor immune responses in both the tumor-draining lymph nodes and tumors in MC38-syngeneic mice models. …”
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Impact of prior SARS-CoV-2 infection and COVID-19 vaccination on the subsequent incidence of COVID-19: a multicentre prospective cohort study among UK healthcare workers – the SIRE... by Nick Andrews, Mary Ramsay, Maria Zambon, Victoria Hall, Sarah Wallace, Susan Hopkins, Jean Timeyin, Tim J G Brooks, Amoolya Vusirikala, Meera Chand, Andre Charlett, Sakib Rokadiya, Colin S Brown, Madhumita Shrotri, Peter D Kirwan, Michele Cole, Natalie Gillson, Ana Atti, Sarah Foulkes, Andrew Taylor-Kerr, Blanche Oguti, Jasmin Islam

“…Secondary outcomes include incidence and prevalence (both RNA and antibody) of SARS-CoV-2, viral genomics, viral culture, symptom history and antibody/neutralising antibody titres.Ethics and dissemination The study was approved by the Berkshire Research Ethics Committee, Health Research Authority (IRAS ID 284460, REC reference 20/SC/0230) on 22 May 2020; the vaccine amendment was approved on 12 January 2021. …”
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Natural Concurrent Infections with Black Spot Disease and Multiple Bacteriosis in Farmed Nile Tilapia in Central Kenya by Daniel W. Wanja, Paul G. Mbuthia, Robert M. Waruiru, Lilly C. Bebora, Helena A. Ngowi

“…Nile tilapia (Oreochromis niloticus) is the most cultured and available fish for Kenyan consumers, and therefore, any tilapine disease deprives them the valuable source of protein. …”
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Methicillin-Resistant Staphylococcus aureus (MRSA): Prevalence and Antimicrobial Sensitivity Pattern among Patients—A Multicenter Study in Asmara, Eritrea by Eyob Yohaness Garoy, Yacob Berhane Gebreab, Oliver Okoth Achila, Daniel Goitom Tekeste, Robel Kesete, Robel Ghirmay, Ruta Kiflay, Thomas Tesfu

“…Isolation of S. aureus was based on culture and biochemical profiles. Standard antimicrobial disks representing multiple drug classes were subsequently set for oxacillin, gentamicin, erythromycin, and vancomycin. …”
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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress by Yong-Juan Mo, Yi-Li Gao, Shi-Jjian Xu, Bin Wang, Jingping Wu, Peng Yu, Ming Li, Xiangtao Kang, Zhi-Qiang Han

“…High salt stress is known to induce muscle atrophy and alter the physical and chemical properties of muscle in cultured fish. Conversely, an optimal salinity level can enhance muscle quality. …”
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The ABC transporter A7 modulates neuroinflammation via NLRP3 inflammasome in Alzheimer’s disease mice by Irene Santos-García, Pablo Bascuñana, Mirjam Brackhan, María Villa, Ivan Eiriz, Thomas Brüning, Jens Pahnke

“…In primary glial cell cultures of APPPS1-hA7ko microglia and APPPS1 astrocytes from newborn pups, we observed that conditioned media from LPS-stimulated microglia was able to induce NLRP3 inflammasome expression and proinflammatory cytokine release in astrocytes. …”
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Gut bacterium Intestinimonas butyriciproducens improves host metabolic health: evidence from cohort and animal intervention studies by Elena Rampanelli, Nadia Romp, Antonio Dario Troise, Jakshana Ananthasabesan, Hao Wu, Ismail Sahin Gül, Sabrina De Pascale, Andrea Scaloni, Fredrik Bäckhed, Vincenzo Fogliano, Max Nieuwdorp, Thi Phuong Nam Bui

“…We also investigated the fructoselysine degradation capacity within the Intestinimonas genus using a culture-dependent approach and found that I. butyriciproducens is a key player in the butyrogenic fructoselysine metabolism in the gut. …”
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Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein by Maliki Ankavay, Nathalie Da Silva, Angela Pollán, Noémie Oechslin, Katja Dinkelborg, Patrick Behrendt, Darius Moradpour, Jérôme Gouttenoire

“…Conclusions: We describe a convenient, quantitative and potentially scalable system for the monitoring of HEV infection and replication in tissue culture. Impact and implications:: Hepatitis E virus infection is one of the most frequent causes of acute hepatitis and jaundice worldwide. …”
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Pediatric bacterial meningitis in southern China: analysis of 838 cases by Lianfeng Chen, Wen-Lin Wu, Yuanyuan Gao, Xiaojing Li, Sida Yang, Huici Liang, Kelu Zheng, Yani Zhang, Haixia Zhu, Yang Tian, Bingwei Peng, Haisheng Lin, Xiuying Wang, Shuyao Ning, Yinyan Gan, Chi Hou, Yinting Liao, Huiling Sheng, Wen-Xiong Chen, Wen-Xiong Chen

“…Furthermore, 38.7% of the patients had positive bacterial cultures of blood or CSF, with Streptococcus agalactiae, Escherichia coli, and Streptococcus pneumoniae commonly seen. …”
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The Impact of Optical Undersampling on the Ca2+ Signal Resolution in Ca2+ Imaging of Spontaneous Neuronal Activity by Katarina D. Milicevic, Violetta O. Ivanova, Tina N. Brazil, Cesar A. Varillas, Yan M.D. Zhu, Pavle R. Andjus, Srdjan D. Antic

“…Methods: Primary neuronal cultures were prepared from the cortex of newborn pups. …”
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Depth-Dependent Cellular Response from Dental Bulk-Fill Resins in Human Dental Pulp Stem Cells by Su-Min Lee, Soo-Youn Kim, Jae-Heon Kim, Soo-Kyung Jun, Hae-Won Kim, Jung-Hwan Lee, Hae-Hyoung Lee

“…The resin samples from the three layers were separated and eluted in the culture medium. The extracts were exposed to hDPSCs, and cytotoxicity and differentiation capability were evaluated. …”
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Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa by Mojisola Clara Hosu, Sandeep Vasaikar, Grace Emily Okuthe, Teke Apalata

“…Samples collected from abattoir wastewater and aquatic environment were processed by membrane filtration and cultured on CHROMagarTM Pseudomonas medium. Species identification was performed by autoSCAN-4 (Dade Behring Inc., IL). …”
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Salinity effect evaluation on the survival rate and hematology of snakeskin gourami juvenile Trichopodus pectoralis by Lies Setijaningsih

“…The increase of its production of culture was still constrained by the slow rate of growth and survival rate in the seed phase. …”
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Prevalence, Antimicrobial Susceptibility Pattern of Bacterial Isolates, and Associated Factors of Urinary Tract Infections among HIV‐Positive Patients at Hiwot Fana Specialized Uni... by Dadi Marami, Senthilkumar Balakrishnan, Berhanu Seyoum

“…Clean-catch midstream urine samples were collected aseptically and examined using the recommended culture methods. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disk diffusion technique. …”
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Circulations migratoires des peuples de l’Ouest Cameroun : impacts culturels et cultuels by Gisèle PIEBOP

“…Le contact d’hommes et incidemment de langues et de cultures étant un phénomène inéluctable, qui plus est dans le contexte de mondialisation ambiant qui met en avant les mouvements des hommes et biens, il s’en suit des influences tous azimuts qu’il n’est pas inopportun d’explorer. …”
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iPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis by Jingyu Zhao, Jingyu Zhao, Wei Fang, Yangjie Gao, Jiquan Chen, Guizhen Wang, Julin Gu

“…Amphotericin B (AmB) serves as the cornerstone treatment for CM; however, its clinical application is restricted due to limited penetration of the blood–brain barrier and associated nephrotoxicity.ObjectiveThis study investigates the use of exosomes derived from induced pluripotent stem cells (iPSC-Exos) as carriers for AmB in treating CM, aiming to enhance therapeutic efficacy and safety and reduce AmB toxicity.MethodsExosomes were extracted from iPSC culture supernatants using ultrafiltration and ultracentrifugation. …”
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REDD1 mediates HDM-induced nuclear-cytoplasmic translocation and release of IL-33 in airway epithelial cells by downregulating Nrf2 by Tian Luo, Wentao Ji, Yuxin Gong, Lichang Chen, Chao Liu, Dandan Zhang, Xi Li, Yanhua Lv

“…In vitro, 24-hour exposure to HDM induced a notable nuclear-to-cytoplasmic translocation of IL-33 and increased its levels in the culture medium of 16HBE cells. In addition, HDM treatment significantly upregulated the expression of both REDD1 and Nrf2. …”
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Expectation and Satisfaction with Nursing Care among Hypertensives Receiving Care at a Resource-Constrained Hospital in Ghana by Kennedy Dodam Konlan, Mavis Armah-Mensah, Rita Aryee, Theresa Akua Appiah

“…Further, the respondents identified some key areas of dissatisfaction and these included the responsiveness of nurses to patient needs, prompt pain management, effectiveness of health education, and provision of culturally sensitive communication. Disproportionate distribution of nursing staff across the three nursing shifts, unethical practice among some nurses, inadequate resources for work, and low work morale of some nurses were identified as factors responsible for the gaps between patient expectations and actual care received. …”
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The development and validation testing of a comprehensive frailty assessment in women with breast cancer by Sheng-Miauh Huang, Ling-Ming Tseng, Chi-Cheng Huang, Pei-Ju Lien, Su-Chen Fang, Yinhui Hong

“…Further assessments of its reliability, validity, and generality from health providers’ views in different contexts and cultures are recommended.…”
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Bacterial Profile and Antibiotic Susceptibility Pattern of Urinary Tract Infection among Pregnant Women Attending Antenatal Care at a Tertiary Care Hospital in Southern Ethiopia by Ashenafi Tula, Abraham Mikru, Tsegaye Alemayehu, Beyene Dobo

“…Midstream urine was collected from pregnant women using sterile containers. Culture and sensitivity were performed using a standard operating procedure of the microbiology laboratory. …”
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