Use of standardized nasal and skin decolonization to reduce rates of bacteremia in patients undergoing extracorporeal membrane oxygenation by Madyson Taylor, Russell L Griffin, Jeremey Walker, Catina James, Angela Akinsanya, Mary Duncan, Rachael A Lee

“…The primary outcome was rate of ECMO-attributable positive blood cultures. Zero-inflated Poisson regression analysis was performed to estimate rate ratios (RRs) for the association between decolonization with BSI rates. …”
Get full text

Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam by Huan Vinh Dong, Paul Adamson, Dieu Hoa Pham, Quynh Hoa Pham, Hai Ha Long Le, Loc Quang Pham, Hao Thi Minh Bui, Giang Minh Le, Jeffrey D. Klausner

“…Participants self-collected oral specimens using phosphate buffer solution, for culture on LB agar media containing sucrose, vancomycin, and trimethoprim (LBVT.SNR). …”
Get full text

Protocol for a randomised ‘screen-and-treat’ Helicobacter pylori eradication trial in 14–18-years-old adolescents residing in three regions of Chile: effectiveness and microbiologi... by Ximena Aguilera, Camila Cabrera, Sergio George, Yalda Lucero, Beatriz Zabala Torres, Lilian Fernández, Nora Mamani, Anne Lagomarcino, Miguel O'Ryan

“…Stool samples will be employed for Escherichia coli and Enterococcus spp—culture, assessing AMR via the disk diffusion method. …”
Get full text

Unlocking barriers and strategies of halal certification for micro and small enterprises in Indonesia: Analytic network process approach by Muhammad Anwar Fathoni, Prima Dwi Priyatno, Faizi, Fadhli Suko Wiryanto, Widarto Rachbini, Suryani

“…Acknowledgment The authors would like to express their sincere gratitude to the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia (Kemendikbudristek RI) for providing financial support for this investigation. …”
Get full text

Paracrine Proangiogenic Function of Human Bone Marrow-Derived Mesenchymal Stem Cells Is Not Affected by Chronic Kidney Disease by Femke C. C. van Rhijn-Brouwer, Bas W. M. van Balkom, Diana A. Papazova, Diënty H. M. Hazenbrink, Anke J. Meijer, Isaac Brete, Vidalmar Briceno, Arjan D. van Zuilen, Raechel J. Toorop, Joost O. Fledderus, Hendrik Gremmels, Marianne C. Verhaar

“…While the immunomodulatory capacity of CKD BM MSCs has been established, it is unknown whether CKD affects wound healing and angiogenic potential of MSC-derived CM and EVs. Methods. MSCs were cultured from BM obtained from kidney transplant recipients (N=15) or kidney donors (N=17). …”
Get full text

Aloe Emodin Reduces Cardiac Inflammation Induced by a High-Fat Diet through the TLR4 Signaling Pathway by Yingfu Chen, Burong Feng, Ye Yuan, Juan Hu, Wei Zhao, Huiwei Jiang, Wen Li, Ziyi Fan, Zhimin Du

“…We used a mouse model of hyperlipidemia caused by a high-fat diet (HFD) for 10 weeks and cell culture experimental models of inflammation in the heart stimulated by PA for 14 h. …”
Get full text

Carboplatin-resistance-related DNA damage repair prognostic gene signature and its association with immune infiltration in breast cancer by Shuwen Dong, Anqi Li, Ruixin Pan, Jin Hong, Zheng Wang, Kunwei Shen

“…This study aimed to identify the DDR genes involved in carboplatin resistance and to elucidate their effects on prognosis, immune infiltration, and drug sensitivity in breast cancer patients.MethodsA 3D-culture model resistant to carboplatin was constructed and sequenced. …”
Get full text

Moscatilin inhibits vascular calcification by activating IL13RA2-dependent inhibition of STAT3 and attenuating the WNT3/β-catenin signalling pathway by Tingting Zhang, Mengmeng Zhu, Jialing Ma, Zhenghong Liu, Zhidan Zhang, Meijie Chen, Yaping Zhao, Huaxin Li, Shengnan Wang, Xiaoning Wei, Wenwen Zhang, Xiaoxiao Yang, Peter J. Little, Danielle Kamato, Hao Hu, Yajun Duan, Baotong Zhang, Jianbo Xiao, Suowen Xu, Yuanli Chen

“…The protective effects of Moscatilin against vascular calcification were verified in cultured HASMCs. Unbiased transcriptional profiling analysis and cellular thermal shift assay suggested that Moscatilin suppresses vascular calcification via binding to interleukin 13 receptor subunit A2 (IL13RA2) and augmenting its expression. …”
Get full text

Effects of Dietary Inclusion of Canthaxanthin- and α-Tocopherol-Loaded Liposomes on Growth and Muscle Pigmentation of Rainbow Trout (Oncorhynchus mykiss) by Tran Quoc Toan, Viet Anh Dang, Quoc Long Pham, Phi Hung Nguyen, Thu Huong Trinh, Thuy Ha Tran, Van Thinh Do, Van Khoi Le, Xuan Luong Ngo, Tri Nhut Pham, Hai Ha Pham Thi, Manh Do Van, Duy Chinh Nguyen, Thanh Duong Nguyen

“…In this study, we included canthaxanthin- and α-tocopherol-loaded liposomes into fish diets and evaluated the effects of supplemented fish feed on fish growth, color, nutrition, and canthaxanthin deposition in fillets of cultured rainbow trout (Oncorhynchus mykiss). The liposomes were fabricated using lecithin as phospholipids with the initial concentrations (IC = mcanthaxanthin/mlipids, % wt/wt) of canthaxanthin at 0.1%, 0.5%, and 1.0%. …”
Get full text

Effects of Different Intertillage Practices on Soil Biochemical Properties and Soybean Yield in Soybean Fields by Mingcong Zhang, Yanhong Zhou, Chenglin Li, Cheng Yuan, Mingfen Shan, Yuxin Fan, Zhongxia Yu, Linfeng Ren, Liu Cui, Chen Wang

“…In 2021, the effects of intertillage on soil biochemical characteristics and soybean yield formation were systematically analyzed under the T5 treatment (subsoiling at the stage of full development from the fourth to the fifth compound leaf) and the T6 treatment (soil culture at the stage of full development from the fourth to the fifth compound leaf). …”
Get full text

Understanding and measuring workplace violence in healthcare: a Canadian systematic framework to address a global healthcare phenomenon by Christian Schulz-Quach, Brendan Lyver, Charlene Reynolds, Trevor Hanagan, Jennifer Haines, John Shannon, Laura Danielle Pozzobon, Yasemin Sarraf, Sam Sabbah, Sahand Ensafi, Natasha Bloomberg, Jaswanth Gorla, Brendan Singh, Lucas B. Chartier, Marnie Escaf, Diana Elder, Marc Toppings, Brian Hodges, Rickinder Sethi

“…By applying a systems-based approach that incorporates trauma-informed strategies and fosters a culture of mutual respect, institutions can develop strategies to minimize WPV and promote a safer work environment for patients, families, staff, and HCPs.…”
Get full text

Clinical and Mycobacterium tuberculosis strain characteristics of tuberculosis patients with diabetes mellitus in Changping District, Beijing, China by Xiaolong Cao, Zexuan Song, Ping He, Xinyue Li, Nan Lei, Qian Sun, Xue Wang, Ruida Xing, Bing Zhao, Xinyu Yang, Zhiguo Zhang, Yanlin Zhao

“…Methods Whole genome sequencing (WGS) and drug susceptibility tests (DST) were performed on culture-positive strains. Spearman correlation analysis was used to examine risk factors and the correlation between lineage, cavities, and hemoptysis in the TB-DM population. …”
Get full text

Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by Bacteroides and their extracellular vesicles by Basit Yousuf, Walid Mottawea, Galal Ali Esmail, Nazila Nazemof, Nour Elhouda Bouhlel, Emmanuel Njoku, Yingxi Li, Xu Zhang, Zoran Minic, Riadh Hammami

“…Cell-free supernatants from wild-type (WT, GABA+) and ΔgadB (GABA-) provided GABA-independent reinforcement of epithelial membrane integrity in LPS-treated Caco-2/HT29-MTX co-cultures. EVs from WT and ΔgadB attenuated inflammatory immune response of LPS-treated RAW264.7 macrophages, with reduced pro-inflammatory cytokines (IL-1β and IL-6), downregulation of TNF-α, and upregulation of IL-10 and TGF-β. …”
Get full text

Manganese-based immunotherapy synergized with novel supramolecular hydrogel: Advancing localized immune activation strategies in squamous cell carcinoma by Tianqing Wang, Xiaopei Hu, Xin Tian, Ying Wang, Shiyu Zhang, Silu Sun, Ning Ji, Xiaobo Luo, Qianming Chen, Xin Zeng, Hang Zhao, Jing Li

“…Firstly, we used a tumor-immune cell co-culture system to demonstrate that Mn2+ enhances immune cytotoxicity in HNSCC via cGAS-STING activation. …”
Get full text

Selective suppression of influenza A/H5N1 virus replication <i>in vitro</i> using nanocomplexes consisting of siRNA and aminopropylsilanol nanoparticles by Marina N. Repkova, Asya S. Levina, Oleg Yu. Mazurkov, Elena V. Makarevich, Ekaterina I. Filippova, Natalya A. Mazurkova, Valentina F. Zarytova

“…The most effective nanocomplex that contained siRNA targeted at the chosen region of mRNA segment 5 of the viral genome reduced virus replication in the culture by a factor of 630. We have shown that non-agglomerated and water-soluble aminopropylsilanol nanoparticles are low-toxic, capable of delivering siRNA into cells and protecting siRNA in the Si~NH2/siRNA nanocomplexes from hydrolysis by cellular nucleases. …”
Get full text

Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’ by Shraddha Maitra, Bruce Dien, Kristen Eilts, Nurzhan Kuanyshev, Yoel R. Cortes-Pena, Yong-Su Jin, Jeremy S. Guest, Vijay Singh

“…The industrial strain grew and fermented efficiently under low aeration conditions having an ethanol titer, yield, specific and volumetric productivities of 46.96 ± 0.19 g/l, 0.51 ± 0.00 g/g, 0.27 ± 0.02 g/g.h and 1.95 ± 0.01 g/l.h, respectively, while the lab strain grew better under higher aeration conditions having the ethanol titer, yield, specific and volumetric productivities of 24.93 ± 0.09, 0.27 ± 0.00 g/g, 0.17 ± 0.00 g/g.h and 1.04 ± 0.00 g/l.h, respectively. Acclimation of cultures in a blended medium significantly improved the performance of the yeast strains. …”
Get full text

Pharmacological Preconditioning Improves the Viability and Proangiogenic Paracrine Function of Hydrogel-Encapsulated Mesenchymal Stromal Cells by Francesco K. Touani, Melanie Borie, Feryel Azzi, Dominique Trudel, Nicolas Noiseux, Shant Der Sarkissian, Sophie Lerouge

“…Paracrine function was studied first by quantifying the proangiogenic growth factors released, followed by assessing scratched HUVEC culture wound closure velocity and proliferation of HUVEC when cocultured with encapsulated hMSC. …”
Get full text

Establishment and evaluation of a UPLC-MS/MS method for simultaneous determination of bongkrekic acid and dehydroacetic acid in rice noodles by Lingguo Zhao, Ronggui Lv, Ronggui Lv, Jiang Song, Xi Chen, Lei Lei, Weixian Zeng, Miaowen She, Miaowen She, Dabing Li, Xiaxia Yu, Zhanguo Liu, Xiao Wang, Yong Liu

“…To investigate the cause of death of the poisoned patient, the toxigenic bacterium Burkholderia gladiolus was isolated and cultured to produce BA. The results showed that the established method could also be used to qualitatively screen BA and its isomer isobongkrekic acid (IBA) in GVC enrichment broth, potato glucose agar and rice sample, which facilitates a rapid and accurate judgement of whether the isolated bacterium strain produces BA. …”
Get full text

Shramahara Mahakasya, a traditional polyherbal formulation, induces anti-anxiety activity in hippocampal neurons by effectuating SOD2-mediated protection against oxidative stress by Saakshi Saini, Viney Kumar, Swati Haldar, Samrat Chauhan, Pratibha Demiwal, Souvik Ghosh, Sumeet Gupta, Debabrata Sircar, Bidhan Mahajon, Partha Roy

“…In this study, we investigated the intricate mechanisms through which SM polyherbal extract exerts neuroprotective and anxiolytic effects in cultured HT-22 cells and rodent models. Method: In this study, the chemical composition of SM was first identified using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). …”
Get full text

Concentration-dependent effects of immunomodulatory cocktails on the generation of leukemia-derived dendritic cells, DCleu mediated T-cell activation and on-target/off-tumor toxici... by Hazal Aslan Rejeski, Hazal Aslan Rejeski, Anne Hartz, Anne Hartz, Elias Rackl, Elias Rackl, Lin Li, Lin Li, Christoph Schwepcke, Christoph Schwepcke, Kai Rejeski, Kai Rejeski, Christoph Schmid, Christoph Schmid, Andreas Rank, Andreas Rank, Jörg Schmohl, Doris Kraemer, Peter Bojko, Helga Maria Schmetzer, Helga Maria Schmetzer

“…For Kit-M, we noted optimal generation of DC-subsets at intermediary concentration ranges of PGE1 (0.25-4.0 µg/mL), which facilitated upregulation of activated and memory T-cells upon mixed lymphocyte culture, and efficient anti-leukemic activity in cytotoxicity assays. …”
Get full text