<i>Chlamydia trachomatis</i> Serovars from the C-Complex and the B- and C-Related Complexes Are Significantly More Pathogenic than Those from the B-Complex in C3H/HeN but Not in BA... by Sukumar Pal, Jennifer R. Carmichael, Delia F. Tifrea, Olga Tatarenkova, Luis M. de la Maza

“…Mice were infected transcervically with 10⁵ inclusion forming units of each of the <i>C. trachomatis</i> serovars, and vaginal cultures were collected. To determine the pathogenicity and its impact on fertility, at week seven post-infection, female mice were caged with male mice. …”
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Astaxanthin Protects Primary Hippocampal Neurons against Noxious Effects of Aβ-Oligomers by Pedro Lobos, Barbara Bruna, Alex Cordova, Pablo Barattini, Jose Luis Galáz, Tatiana Adasme, Cecilia Hidalgo, Pablo Muñoz, Andrea Paula-Lima

“…Primary hippocampal cultures were incubated with 0.1 μM ATX for 1.5 h prior to AβOs addition (500 nM). …”
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Coping with sand and dust storms: Developing and validating of an adaptation assessment tool by Shiva Salehi, Ali Ardalan, Abbas - Ostadtaghizadeh, Armin Zareiyan, Gholamreza Garmaroudi, Abbas Rahimiforoushani

“…Achieving such a goal requires the use of reliable, stable, and indigenous culture-based tools. The purpose of this study is to design and psychometrically assess the adaptation of Iranian society to dust. …”
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Frequency of Lower Lung Field Tuberculosis in Diabetes Mellitus Patients Attending Tertiary Care Hospital in Bangladesh: A Cross‐Sectional Study by Raihan Kamal Galib, Susanta Kumar Paul, Khujjista Akter, Manzurul Ibrahim Musa, Dip Jyoti Sarker, Shah Ashiqur Rahman Ashiq Choudhury, Shipan Chandra Paul, Rajashish Chakrabortty

“…PTB was diagnosed by positive sputum acid‐fast bacilli (AFB),/Culture,/Gene Xpert MTB/RIF, and DM, which was proven by taking oral hypoglycemic drugs or receiving insulin at the time of hospital admission. …”
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Epicardially Placed Bioengineered Cardiomyocyte Xenograft in Immune-Competent Rat Model of Heart Failure by Ikeotunye Royal Chinyere, Pierce Bradley, Joshua Uhlorn, Joshua Eason, Saffie Mohran, Giuliana G. Repetti, Sherry Daugherty, Jen Watson Koevary, Steven Goldman, Jordan J. Lancaster

“…Methods. hiPSC-CMs and human dermal fibroblasts were cultured into a synthetic bioabsorbable scaffold. The engineered grafts underwent epicardial implantation in infarcted immune-competent male Sprague-Dawley rats. …”
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Increased dicentric chromosome in peripheral lymphocytes is related to acute skin toxicity induced by radiotherapy in cancer patients by Minjie Li, Yu Gao, Lin Han, Ping Wang, Jie Li, Junhua Song, Shasha Du, Yunfei Zhang, Yumin Lyu

“…Peripheral blood samples were collected from each patient before and after RT, followed by whole-blood lymphocyte culture and chromosome analysis. Dicentric chromosomes were searched automatically using a high-throughput chromosome analysis system and were then confirmed manually. …”
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Clinical validation and optimization of machine learning models for early prediction of sepsis by Xi Liu, Meiyi Li, Xu Liu, Yuting Luo, Dong Yang, Hui Ouyang, Jiaoling He, Jinyu Xia, Fei Xiao, Fei Xiao, Fei Xiao

“…Early prediction of sepsis onset can drive effective interventions and improve patients’ outcome.MethodsData were collected retrospectively from a cohort of 2,329 adult patients with positive bacteria cultures from a tertiary hospital in China between October 1, 2019 and September 30, 2020. …”
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Isolation and molecular detection of Peste des petits ruminants virus in an outbreak investigation in Benishangul Gumuz, Ethiopia by Mengesha Abebe, Yimer Lama, Aliy Abdi, Shegu Dereje, Abdeta Debela, Waktole Hika, Hirpa Tola Eyob

“…Altogether, 180 clinically diagnosed and 32 shoats with typical clinical sign of PPR were identified for laboratory confirmation Nasal and rectal swabs from 32 sheep and goats were collected and cultured on Verodog SLAM (VDS) cell line and tested using then tested using a quantitative real-time polymerase chain reaction (RT_PCR) assay and conventional PCR with 1.5% gel electrophoresis for detection of the N gene. …”
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Atypical Microbiological Feature of Infectious Endophthalmitis on Jeju Island: A 10-Year Study at a Single Tertiary Referral Center by Joong Hyun Park, Dong Yoon Kim, Ahnul Ha, Dae Joong Ma, Hye Jin Lee, Jinho Jeong, Jin Young Kim

“…The baseline characteristics of all subjects were investigated, and a culture examination was performed. The isolated pathogens were analyzed to determine how their microbiological features differed from those in regions with different geographical conditions. …”
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Assessment of Protein Quality and Nutritional Characteristics of Commonly Consumed Pulses in the Caribbean Diet by Different In Vitro Assays by Daniel J. Thomas, Zhanhui Lu, Yolanda Brummer, Yan Zhu, Ronghua Liu, Lili Mats, Rong Tsao, D. Dan Ramdath, Matthew G. Nosworthy

“…Pulses, the dried seeds of leguminous plants, form an important part of the diets of many cultures, including Caribbean cuisine, and are a rich source of protein, carbohydrates, and antioxidants while being low in fats. …”
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Ensiling and thermic treatment effects on ruminal carbohydrate fermentation and post-ruminal crude protein concentration in partial-crop peas and faba beans by M. Bachmann, P. Okon, F. Pilger, C. Kuhnitzsch, S.D. Martens, O. Steinhöfel, A. Zeyner

“…This study was conducted to examine effects of ensiling, toasting or ensiling plus toasting in partial-crop field peas and faba beans harvested each with 375 g dry matter/kg (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie (BBCH) scale 79 and 81, respectively) on gas production and post-ruminal crude protein (PRCP) concentration by in vitro incubation in ruminal fluid batch-cultures. The silages made from partial-crop field peas and faba beans (Rostock Model Silages) had a pH of 4.3 and 4.6, respectively, and were not typically lactic acid dominated. …”
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Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments by Amit Verma, Vikas Pandey, Catherine Sherry, Taylor Humphrey, Christopher James, Kailie Matteson, Jason T. Smith, Alena Rudkouskaya, Xavier Intes, Margarida Barroso

“…The study demonstrates that FLI is a powerful analytical tool to monitor the delivery of antibodydrugs both in cell cultures and in vivo live systems. Especially, MFLI FRET is a unique imaging modality that can directly quantify target engagement with the potential to elucidate the role of the TME in drug delivery efficacy in intact live tumor xenografts.…”
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Antifungal efficacy of chitosan-mediated fenugreek nanocomposite incorporated in tissue conditioner by Mohika Damle, Ponsekar Abraham Anandapandian, B. Eswaran, Swapnaneel Pradhan, Silvin Joe C J, Shreyas Dhumke

“…Heat cure denture base discs coated with tissue conditioner-nanocomposite mixture were used to transfer fungal cultures onto Rose Bengal agar to assess colony formation. …”
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Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice by Jiawei Liao, Xiaolei Yang, Qiuyue Lin, Shuang Liu, Yunpeng Xie, Yunlong Xia, Hui-Hua Li

“…In vitro, PYR-41 blunted ox-LDL-induced lipid deposition and expression of proinflammatory cytokines (Il-1β and Il-6) and NADPH oxidases (Nox1, Nox2, and Nox4) in cultured RAW264.7 macrophages. Conclusions. We demonstrated that UBA1 expression was upregulated and mainly derived from macrophages in the atherosclerotic plaques and inactivation of UBA1 by PYR-41 suppressed atherosclerosis development probably through inhibiting macrophage proinflammatory response and oxidative stress. …”
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Infectious Aortitis with Abdominal Aortic Aneurysm in a 47-Year-Old Female with Systemic Lupus Erythematosus by Valerie R. Ramiro, Carmegie C. Saliba, John Anthony D. Tindoc, Marinette R. Jambaro, Enrique M. Chua, Donna Ricca M. Hornilla, Maria Teresa B. Abola

“…Intraoperative findings were a saccular infrarenal aneurysm with dissection up to the proximal right common iliac artery and an abscess compartment within the false lumen in the anterior aortic wall. Abscess culture yielded high growth of Salmonella group B. …”
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Clofarabine induces tumor cell apoptosis, GSDME-related pyroptosis, and CD8+ T-cell antitumor activity via the non-canonical P53/STING pathway by Jing Chen, Jie Wu, Xiang Chen, Qian Tao, Nian Liu, Cong Peng, Can Lu, Qiuqiu Li

“…The molecular mechanisms of Clo-induced tumor cell death were analyzed using western blotting, ELISA, reverse transcription-PCR, immunofluorescence, co-immunoprecipitation (CO-IP), short hairpin RNA, co-culture, chromatin immunoprecipitation, and flow cytometry. …”
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Crude Drugs for Clearing Heat Contain Compounds Exhibiting Anti-Inflammatory Effects in Interleukin-1β-Treated Rat Hepatocytes by Airi Fujii, Saki Onishi, Nodoka Watanabe, Mizuki Kajimura, Kentaro Ito, Keita Minamisaka, Yuto Nishidono, Saki Shirako, Yukinobu Ikeya, Mikio Nishizawa

“…All the EtOAc-soluble fractions, except that from <i>Forsythia</i> fruits, inhibited interleukin (IL)-1β-induced nitric oxide (NO) production in primary-cultured rat hepatocytes. Anti-inflammatory compounds were identified from these EtOAc-soluble fractions: baicalein from <i>Scutellaria</i> roots, (−)-nyasol from <i>Anemarrhena</i> rhizomes, and loniflavone from <i>Lonicera</i> leaves and stems. (+)-Phillygenin was purified from <i>Forsythia</i> fruits by removing cytotoxic oleanolic and betulinic acids. …”
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TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis by Lin Wang, Diqing Ouyang, Lin Li, Yunchuan Cao, Yingwen Wang, Nina Gu, Zhaosi Zhang, Zhao Li, Shuang Tang, Hui Tang, Yuan Zhang, Xiaochuan Sun, Jin Yan

“…Various techniques were used to assess the role of DAM-like cells in TBI and the effects of glycolysis on DAM-like cells, including RT‒qPCR, immunofluorescence assays, behavioural tests, extracellular acidification rate (ECAR) tests, Western blot analysis, cell magnetic sorting and culture, glucose and lactate assays, and flow cytometry. …”
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Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer by Juan José Mora Román, Miguel Del Campo, Javiera Villar, Francesca Paolini, Gianfranca Curzio, Aldo Venuti, Lilian Jara, Jorge Ferreira, Paola Murgas, Alvaro Lladser, Augusto Manubens, María Inés Becker

“…Hemocyanins, as opposed to QS-21, had no cytotoxic effect on either oral cancer cell line cultured in vitro, supporting the idea that the antitumor effects of hemocyanins are associated with their modulation of the immune response. …”
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Can enhanced CSR quality reduce the cost of debt capital? An empirical analysis of CEO expertise and non-financial reporting practices in China by Oleh Pasko, Yang Zhang, Nelia Proskurina, Vadym Sapych, Yelyzaveta Mykhailova

“…AcknowledgmentThis paper is co-funded by the European Union through the European Education and Culture Executive Agency (EACEA) within the project “EU Best Practice of Life Cycle Assessment, Social, Environmental Accounting and Sustainability Reporting” – 101047667-ERASMUS-JMO-2021-MODULE https://jm.snau.edu.ua/en/eu-best-practice-of-life-cycle-assessment-social-environmental-accounting-and-sustainability-reporting/ Oleh Pasko expresses sincere gratitude for the support from the Kirkland Research Program, generously provided by the Leaders of Change Foundation established by the Polish-American Freedom Foundation.…”
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