Surveillance of tick-borne encephalitis virus foci in Slovakia: A seroprevalence study in ruminants combined with virus detection in ticks by Martina Ličková, Bronislava Víchová, Markéta Derdáková, Monika Sláviková, Sabína Fumačová Havlíková, Dana Zubriková, Diana Selyemová, Michal Chvostáč, Lucia Blaňarová, Viktória Čabanová, Veronika Vaňová, Lucia Šulejová, Jana Kerlik, Tomáš Szemes, Katarína Šoltys, Boris Klempa

“…Positive ticks (all I. ricinus) and milk samples were then used for virus isolation. Three new cell culture isolates were prepared and sequenced. Obtained full-length genome sequences revealed high genetic diversity and phylogenetic clustering with virus strains found across Europe. …”
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Global variation in the assessment of psychological trauma in pregnancy by Kathryn Wall, Francesca Penner, Lindsey Wallace Goldman, Jin Young Shin, Eloise Novak, Amanda Lowell, Michèle Day, Lea Papa, Drew Wright, Soudabeh Givrad, Helena J. V. Rutherford

“…The results of this review emphasize the need for regionally specific and culturally appropriate measures of psychological trauma for pregnant people, which prioritize the types of psychological trauma that are most common in a given region. …”
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Blocking the CD39/CD73 pathway synergizes with anti-CD20 bispecific antibody in nodal B-cell lymphoma by Carsten Müller-Tidow, Peter Dreger, Joseph Kauer, Wolfgang Huber, Clara Kolbe, Berit Brinkmann, Sascha Dietrich, Tobias Roider

“…In this study, we applied an autologous ex vivo culture model of primary lymph node-derived T cells to investigate the potential of anti-CD39 or anti-CD73 blocking antibodies as T-cell enhancing combination partners of an anti-CD20 BsAb. …”
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Anti-TNBC effects of Lappaol F by targeting epithelial-mesenchymal transition via regulation of GSK-3β/YAP/β-catenin and PI3K/AKT pathways by Qiqi Meng, Qiqi Meng, Zhiping Li, Xiaofeng He, Yuanhao Hu, Guiyun Wu, Jiawen Huang, Zhuohui Luo, Zhuohui Luo, Yingjie Hu, Xiaoling Shen

“…The present study aimed to reveal the potential mechanism of LAF against TNBC by in vivo and in vitro experiments.MethodsIn situ, transplantation-induced MDA-MB-231 solid tumor model in NCG mice and cultured MDA-MB-231 and Hs-578T cells were used to evaluate the anti-TNBC effect of LAF. …”
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Carbon Monoxide-Releasing Molecule-3 Suppresses Tumor Necrosis Factor-α- and Interleukin-1β-Induced Expression of Junctional Molecules on Human Gingival Fibroblasts via the Heme Ox... by Jia Lv, Yongsheng Liu, Shuhan Jia, Yuna Zhang, Haoyang Tian, Jingyuan Li, Hui Song

“…In this study, we aimed to investigate the effects of CORM-3 on the expression of tight and adherens junction molecules on human gingival fibroblasts (HGFs) stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). HGFs were cultured from the explants of normal human gingival tissues, which were stimulated in the presence or absence of CORM-3. …”
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ADAMTS13 Improves Endothelial Function and Reduces Inflammation in Diabetic Retinopathy by Ahmed M. Abu El-Asrar, Mohd I. Nawaz, Ajmal Ahmad, Mairaj Siddiquei, Eef Allegaert, Lowie Adyns, Lotte Vanbrabant, Priscilla W. Gikandi, Gert De Hertogh, Sofie Struyf, Ghislain Opdenakker

“…We compare human epiretinal membranes and vitreous samples from nondiabetic subjects and patients with proliferative diabetic retinopathy (PDR) and extend in vitro analyses with the use of various immunodetection and spectrofluorimetric methods on rat retina and human retinal glial and endothelial cell cultures. Functional studies include the assessment of the blood–retinal barrier (BRB), cell adhesion, and in vitro angiogenesis. …”
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Hepatic Abnormal Secretion of Apolipoprotein C3 Promotes Inflammation in Aortic Dissection by Xinghui Zhuang, Mohammad Zarif, Yue Shen, Zhaofeng Zhang, Jian He, Linfeng Xie, Qingsong Wu, Xinfan Lin, Keyuan Chen, Yue Tian, Yong Lin, Yuling Zhang, Ziwen Cai, Zhihuang Qiu, Liangwan Chen

“…In vitro, recombinant apo C3 protein was added to the culture medium of J774A.1 macrophages to evaluate its effect on macrophage polarization and to identify the underlying mechanisms. …”
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Reductions in Muscle Strength and Range of Motion Cause Locomotion Disability via Locomotion-Related Functional Limitation in Japanese Older Adults: A Cross-Sectional Study by Hungu Jung, Shigeharu Tanaka, Yuji Iwamoto, Takashi Kawano, Masahiro Yamasaki, Ryo Tanaka

“…To assess locomotion disability, the locomotor domain of the activities of daily living (ADLs) survey from the Ministry of Education, Culture, Sports, Science, and Technology of Japan was used. …”
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Nisin Z Production by Wild Strains of Lactococcus lactis Isolated from Brazilian (Italian Type) Fermented Sausage by Margarete Alice Fontes Saraiva, Dagim Jirata Birri, Dag Anders Brede, Maria Cristina Baracat-Pereira, Marisa Vieira de Queiroz, Ingolf F Nes, Célia Alencar de Moraes

“…Ion exchange and reversed-phase chromatographies were used to purify the bacteriocins from culture supernatant of the five strains. Mass spectrometry (MALDI-TOF/TOF) showed that the molecular masses of the bactericoins from L. lactis ID1.5, ID3.1, ID8.5, PD4.7, and PR3.1 were 3330.567 Da, 3330.514 Da, 3329.985 Da, 3329.561 Da, and 3329.591 Da, respectively. …”
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Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet by Xinyan Zhi, Yixin Gu, Wenli Zhao, Shichao Xie, Yangguang Bao, Sofia Morais, Dong Li, Maolong He, Min Jin, Qicun Zhou

“…The 360 juvenile M. salmoides (13.44 ± 0.03 g) were cultured for 8 weeks in 12 recirculating tanks, 4 per experimental group. …”
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Epidemiology and Antimicrobial Resistance of Stenotrophomonas maltophilia in China, 2014–2021 by Hanli Wang, Shirong Li, Haoyu Ji, Yixin Hu, Susheng Zhou, Xingwu Chen, Zhiwei Lu, Qinghai You, Yusheng Cheng, Lei Zha

“…However, alarmingly high resistance rates were observed in specific specimen types, particularly in blood cultures, suggesting that minocycline may be the only reliable therapeutic option among the six tested antibiotics for treating such infections in China. …”
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The Isolation of Lead-Tolerant PGPR from Red Clover Soil and Its Role in Promoting the Growth of Alfalfa by Wanting Nie, Yuchen Wu, Jingwen Jiang, Zicheng Wang, Meiqi Mu, Siwen Zhao, Minghao Yang, Xi Long, Xiujie Yin, Xiaohua Teng

“…This study used lead-tolerant rhizosphere soil of red clover as experimental material; cultured, isolated, and screened 52 excellent lead-tolerant bacteria that promote rhizosphere growth; and then inoculated them into alfalfa. …”
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Akkermansia muciniphila and its metabolite propionic acid maintains neuronal mitochondrial division and autophagy homeostasis during Alzheimer’s disease pathologic process via GPR4... by Zifan Wang, Cai Wang, Boyu Yuan, Li Liu, Haoming Zhang, Mingqiang Zhu, Hongxia Chai, Jie Peng, Yanhua Huang, Shuo Zhou, Juxiong Liu, Liyong Wu, Wei Wang

“…In order to further reveal the effects and the mechanisms of propionic acid on AD prevention, we systematically explored the effects of propionic acid administration on AD model mice and cultured hippocampal neuronal cells. Results showed that oral propionate supplementation ameliorated cognitive impairment in AD mice. …”
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miR-223 and Chromogranin A Affect Inflammatory Immune Cell Activation in Liver Metastasis of Neuroendocrine Neoplasms by Lukas Geisler, Katharina Detjen, Teresa Hellberg, Marlene Kohlhepp, Carsten Grötzinger, Jana Knorr, Ines Eichhorn, Raphael Mohr, Theresa Holtmann, Bertram Wiedenmann, Frank Tacke, Christoph Roderburg, Alexander Wree

“…Subsequently, in vitro transfections using the well-established human pancreatic NEN cell line (BON), and co-culture experiments with primary macrophages and neutrophils were performed. …”
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Kaempferol protects against high-salt-induced hypertension and vascular endothelial injury by inhibiting ferroptosis through the ATF4/ACSL4 pathway by Qiuer Liang, Minghao Chen, Guangtian Chen, Pengli Xu, Lai Kwan Lam, Pengcheng Xie, Ting Xie, Wanqing Tu, Tianhao Liu, Xiaopeng Peng, Haoyao Yuan, Liguo Chen, Ya Xiao

“…Additionally, human aortic endothelial cells (HAECs) cultured under high-salt conditions were used to assess Kae's protective effects, with ATF4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) knocked down via siRNA transfection. …”
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Evaluation of the relatedness between the biofilm-associated genes and antimicrobial resistance among Acinetobacter baumannii isolates in the southwest Iran by Nafiseh Hosseinzadeh Shakib, Zahra Hashemizadeh, Abolfazl Rafati Zomorodi, Reza Khashei, Yeganeh Sadeghi, Abdollah Bazargani

“…Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and biofilm production potential was assessed through the tissue culture plate (TCP) method. Additionally, we investigated eleven biofilm-related genes (ompA, bap, csuE, epsA, blaper-1, bfmS, pgaB, csgA, fimH, ptk, and kpsMII) in all isolates using polymerase chain reaction (PCR). …”
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Biofilm Formation by Staphylococcus aureus Isolated from Food Contact Surfaces in the Dairy Industry of Jalisco, Mexico by María-Guadalupe Avila-Novoa, Maricarmen Iñíguez-Moreno, Oscar-Alberto Solís-Velázquez, Jean-Pierre González-Gómez, Pedro-Javier Guerrero-Medina, Melesio Gutiérrez-Lomelí

“…The isolates were characterized phenotypically by culture on Congo red agar plates. The ability of the strains to form biofilms was investigated in 96-well flat-bottomed microtiter polystyrene plates. …”
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3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification by Fan Chen, An-Feng Luo, Kai-Xin Pan, Hao Gu, Chang-Fan Zhou, Wei Zeng, Song Liu, Adrian Molenaar, Hong-Yan Ren, Li-Jun Huo, Yan-Zhen Bi

“…In this study, through the in vitro culture of mice embryos, it was initially observed that even low concentrations of PNMC exposure led to a significant reduction in blastocyst formation and a sharp decline in the ratio of inner cell mass within the blastocysts. …”
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Production of subtilisin proteases in bacteria and yeast by A. S. Rozanov, S. V. Shekhovtsov, N. V. Bogacheva, E. G. Pershina, A. V. Ryapolova, D. S. Bytyak, S. E. Peltek

“…., composition of the media, culture duration, and the inf luence of temperature and pH. …”
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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis by Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, Ki-Taek Lim

“…Transcriptomic (RNA-Seq) and real-time PCR (qRT-PCR) study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase (MAPK) signaling pathway after 7 days. …”
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