Successful establishment of CRISPR-based genome-edited clonal cell populations from primary cells of buffalo, goats, and sheep by Priyanka Singh, Bosco Jose, Devika Gautam, Aseem Tara, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…To generate clonal cell populations, the primary fibroblasts were established through explant culture and then electroporated with CRISPR/Cas RNPs targeted for the disrupted MSTN gene. …”
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Stable Isotope-Resolved Metabolomic Differences between Hormone-Responsive and Triple-Negative Breast Cancer Cell Lines by Jason H. Winnike, Delisha A. Stewart, Wimal W. Pathmasiri, Susan L. McRitchie, Susan J. Sumner

“…Methods. Cells were cultured in media containing either [U-13C]-glucose or [U-13C]-glutamine for 48 hours. …”
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Insight into the role of antioxidant in microbial lignin degradation: Ascorbic acid as a fortifier of lignin-degrading enzymes by Aipeng Li, Weimin Wang, Shuqi Guo, Changzhi Li, Xinying Wang, Qiang Fei

“…Thus, AA was selected as model antioxidant and added into the culture medium of T. versicolor to examine the effect of antioxidants on the activity of lignin-degrading enzymes in the fungus. …”
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HIV-Envelope–Dependent Cell-Cell Fusion: Quantitative Studies by Leonor Huerta, Nayali López-Balderas, Evelyn Rivera-Toledo, Guadalupe Sandoval, Guillermo Gómez-Icazbalceta, Carlos Villarreal, Edmundo Lamoyi, Carlos Larralde

“…The presence in HIV-infected individuals of virus strains able to induce syncytia in cultures of T cells is associated with disease progression and AIDS. …”
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A non-aseptic bioprocess for production and recovery of 2,3-butanediol via conversion of crude glycerol and corn steep liquor at pilot-scale by Dimitris Karayannis, Nikos Angelou, Gabriel Vasilakis, Ioannis Charisteidis, Alexandros Litinas, Seraphim Papanikolaou

“…In all fed-batch fermentations, final BDO and acetoin concentration reached ∼80 g/L, while a plateau was observed at ∼68 g/L of BDO. Finally, the culture was carried out efficiently in the pilot-scale reactor (250 L). …”
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<i>In vitro</i> assay of biological activity of a national preparation of macrophage activating factor (GcMAF-RF) by E. V. Levites, S. S. Kirikovich, E. V. Dolgova, A. S. Proskurina, G. S. Ritter, А. A. Ostanin, E. R. Chernykh, S. S. Bogachev

“…The level of NO production was estimated by the accumulation of nitrogen monoxide in the culture supernatants of peritoneal macrophages by the colorimetric method using the Griess reagent. …”
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Fenofibrate Protects Cardiomyocytes from Hypoxia/Reperfusion- and High Glucose-Induced Detrimental Effects by Fabiola Cortes-Lopez, Alicia Sanchez-Mendoza, David Centurion, Luz G. Cervantes-Perez, Vicente Castrejon-Tellez, Leonardo del Valle-Mondragon, Elizabeth Soria-Castro, Victoria Ramirez, Araceli Sanchez-Lopez, Gustavo Pastelin-Hernandez, Wylly Ramses Garcia-Niño, Maria Sanchez-Aguilar, Luz Ibarra-Lara

“…Therefore, the objective of this work was to determine the potential protective effect of fenofibrate in cardiomyocytes exposed to HG, H/R, and HG+H/R. Cardiomyocyte cultures were divided into four main groups: (1) control (CT), (2) HG (25 mM), (3) H/R, and (4) HG+H/R. …”
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Observational study protocol: the faecal microbiome in the acute stage of new-onset paediatric type 1 diabetes in an Irish cohort by Eugene Dempsey, Catherine Stanton, Paul Ross, Colin Patrick Hawkes, Aonghus Lavelle, Carol-Anne O’Shea, Elaine Catherine Kennedy, Fiona Catherine Ross

“…We will develop a microbial culture biobank based on culturomic studies of stool samples from the healthy controls that will support future investigation. …”
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Exosomal PVRL4 Promotes Lung Adenocarcinoma Progression by Enhancing the Generation of Myeloid‐Derived Suppressor Cell‐Secreted TGF‐β1 by Yahai Liang, Jinmei Li, Lihua Zhang, Jinling Zhou, Meilian Liu, Xiaoxia Peng, Weizhen Zheng, Zhennan Lai

“…MDSCs were induced from peripheral blood mononuclear cells (PBMCs) by cytokine or co‐culture with cancer cells. Results LUAD tissues and cells showed high PVRL4 expression, and PVRL4 deficiency suppressed LUAD cell proliferation, invasion, migration, and induced cell apoptosis in vitro, and impeded LUAD growth in vivo. …”
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Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study by Victoria A. Shestakova, Ilya D. Klabukov, Ilya V. Kolobaev, Longfeng Rao, Dmitry A. Atiakshin, Michael A. Ignatyuk, Mikhail E. Krasheninnikov, Bagavdin G. Ahmedov, Sergey A. Ivanov, Peter V. Shegay, Andrey D. Kaprin, Denis S. Baranovskii

“…The obtained nasal chondrocytes were used to seed decellularized scaffolds, which were then cultured in vitro for 7, 14, or 21 days in chondrogenic medium. …”
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Power of emperor in Russian legal discourse of second half of 19th– early 20th centuries: justification and options for interpretation by I. G. Adoneva, Yu. V. Druzhinina

“…The methodological basis of this study is the history of intellectual culture as an analysis of legal ideas and discourses in the context of the second half of the 19th - early 20th centuries. …”
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Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens by Jie Zhang, Anna Li, Chang-qing Yang, Peter Garred, Ying Jie Ma

“…Recombinant CL-12 was purified from cell culture supernatants using a 3-step rapid purification procedure utilizing disposable affinity and ion exchange minicolumns. …”
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Osteoblastic Differentiation and Mitigation of the Inflammatory Response in Titanium Alloys Decorated with Oligopeptides by Aroa Álvarez-López, Raquel Tabraue-Rubio, Rafael Daza, Luis Colchero, Gustavo V. Guinea, Martine Cohen-Solal, José Pérez-Rigueiro, Daniel González-Nieto

“…We also find that RGD is the best-performing peptide in in vitro cell cultures, enhancing adhesion, proliferation, and osteogenic differentiation of mesenchymal stem cells. …”
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<i>Staphylococcus aureus</i> Proteins Implicated in the Reduced Virulence of <i>sarA</i> and <i>sarA/agr</i> Mutants in Osteomyelitis by Karen E. Beenken, Mara J. Campbell, Stephanie D. Byrum, Rick D. Edmondson, Samuel G. Mackintosh, Alan J. Tackett, Mark S. Smeltzer

“…A total of 1039 proteins were detected in conditioned media (CM) from overnight cultures of LAC, and protease-mediated degradation was shown to contribute to the reduced abundance of 224 of these (21.6%) in CM from the <i>sarA</i> and <i>sarA/agr</i> mutants. …”
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An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model by Michael P. Leviton, Juan C. Lacayo, K. Yeon Choi, Nelmary Hernandez-Alvarado, Andrew Wey, Mark R. Schleiss

“…MIP deletion virus replicated with wild-type kinetics in cell culture but was attenuated in nonpregnant guinea pigs, demonstrating reduced viremia and reduced inflammation and histopathology (compared to a control virus with an intact GPCMV MIP gene) following footpad inoculation. …”
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Role of Regulatory B Cells in the Progression of Cervical Cancer by Zhifang Chen, Yuejie Zhu, Rong Du, Nannan Pang, Fengbo Zhang, Di Dong, Jianbing Ding, Yan Ding

“…The level of IL-10 in the culture supernatant of Bregs treated with CpG was significantly higher than that of non-Bregs (P<0.05). …”
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Vesicoureteral Reflux in Children with Accidental Diagnosis of Unilateral Small Size Kidney by Mohsen Mohammadi, Khadijeh Ebrahimi, Soraya Khafri, Maryam Nikpour, Hadi Sorkhi

“…All children were followed for at least for 1 year with urine culture and urinalysis every 1-2 months for detection of UTI. …”
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Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation by Li Chen, Kaikai Shi, Weimin Qiu, Lars Aagaard, Moustapha Kassem

“…The expression of KRAB-dCas9 or VP64-dCas9 was controlled by the presence or absence of doxycycline (Dox) in the cell culturing medium. To demonstrate the functionality of the dCas9-effector hMSC system, we tested controlled expression of alkaline phosphatase (ALP) gene through transfection with the same single ALP sgRNA. …”
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REDD1 expression in podocytes facilitates renal inflammation and pyroptosis in streptozotocin-induced diabetic nephropathy by Siddharth Sunilkumar, Sandeep M. Subrahmanian, Esma I. Yerlikaya, Allyson L. Toro, Edward W. Harhaj, Scot R. Kimball, Michael D. Dennis

“…In contrast with diabetic REDD1+/+ mice, diabetic REDD1−/− mice did not exhibit albuminuria, increased pro-inflammatory factors, or renal macrophage infiltration. In cultured human podocytes, exposure to hyperglycemic conditions promoted REDD1-dependent activation of NF-κB signaling. …”
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Tolterodine is a novel candidate for assessing CYP3A4 activity through metabolic volatiles to predict drug responses by Valentina Stock, Rebecca Hofer, Franziska Lochmann, Vera Spanke, Klaus R. Liedl, Jakob Troppmair, Thierry Langer, Hubert Gstach, Christian Dank, Chris A. Mayhew, Sarah Kammerer, Veronika Ruzsanyi

“…In vitro biotransformation of tolterodine by HepG2 cells overexpressing CYP3A4, CYP2D6 or CYP2C9 was investigated by LC-MS analysis of cell culture supernatant for the non-volatile metabolite, N-dealkylated tolterodine, and PTR-ToF-MS analysis of the headspace for acetone. …”
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