Studies on development of early immunity against type O FMD in naturally susceptible animals by D. V. Mikhalishin, D. A. Lozovoy, V. A. Starikov, Yu. S. El’kina, M. I. Doronin, A. V. Borisov

“…The article presents the results of the research into the formation of humoral immunity in naturally susceptible animals triggered by administration of inactivated emulsion FMD vaccines capable of ensuring early protection against type O FMD. Culture FMD virus of О/Primorsky/2012, О/Saudi Arabia/08 and О/Mongolia/2017 strains was used for vaccine production. …”
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Smoking behavior among Asian Americans during the initial phase of the COVID-19 pandemic: The influence of pandemic stressors and depression by Paula Lozano, Aven Peters, Alia Southworth, Yicklun Mo, Helen Lam, Fornessa T. Randal, Karen E. Kim

“…Conclusions The findings of the study highlight the need for culturally tailored smoking cessation interventions for Asian American communities that address pandemic-related stressors such as discrimination that may trigger cigarette use.…”
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MUC2 expression modulates immune infiltration in colorectal cancer by Christophe M. Raynaud, Ayesha Jabeen, Eiman I. Ahmed, Eiman I. Ahmed, Satanay Hubrack, Apryl Sanchez, Shimaa Sherif, Ahmad A. Al-Shaibi, Bernice Lo, Bernice Lo, Jessica Roelands, Davide Bedognetti, Davide Bedognetti, Wouter Hendrickx, Wouter Hendrickx

“…By employing CRISPR-mediated MUC2 knockout, we investigated the influence of MUC2 on tumor immune infiltration and its interplay with T cells and NK cells enriched peripheral blood mononuclear cells (PBMCs) in 3D spheroid cultures.ResultsWhile MUC2 was more abundant in LS-174T cell line compared to HT-29, its knockout resulted in increased immune infiltration solely in the HT-29 cell line, but not in the LS-174T cell line. …”
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Decoding the mannose receptor-mAb interaction: the importance of high-mannose N-glycans and glycan-pairing by Julia Baumeister, Maximilian Meudt, Sybille Ebert, Frank Rosenau, Boris Mizaikoff, Michaela Blech, Kristina M. J. Aertker, Fabian Higel

“…The high-mannose N-glycan content of mAbs can be influenced during the cell culture process by factors such as cell lines, process conditions, and media. …”
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Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression by V. E. Tvorogova, E. Y. Krasnoperova, A. A. Kudriashov, K. A. Kuznetsova, E. A. Potsenkovskaya, Y. A. Fedorova, L. A. Lutova

“…Recently, we have shown  that  transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. …”
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Increased Duodenal Iron Absorption through Upregulation of Ferroportin 1 due to the Decrement in Serum Hepcidin in Patients with Chronic Hepatitis C by Masanori Sato, Koji Miyanishi, Shingo Tanaka, Akira Sakurada, Hiroki Sakamoto, Yutaka Kawano, Kohichi Takada, Masayoshi Kobune, Junji Kato

“…The FPN mRNA levels and protein levels increased significantly in Caco-2/TC7 cell monolayers cultured in transwells with hepcidin. Lower serum hepcidin-25 levels might upregulate not only FPN protein expression but also mRNA expression in the duodenum and cause iron accumulation in patients with CHC.…”
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Natural Killer Cells from Malignant Pleural Effusion Are Endowed with a Decidual-Like Proangiogenic Polarization by Annalisa Bosi, Silvia Zanellato, Barbara Bassani, Adriana Albini, Alessandra Musco, Maria Cattoni, Matteo Desio, Elisa Nardecchia, Davide Giuseppe D’Urso, Andrea Imperatori, Lorenzo Dominioni, Douglas M. Noonan, Lorenzo Mortara, Antonino Bruno

“…Addition of TGFβ or cell-free pleural fluid to IL-2 in the culture medium abrogated NK cell CD107a and IFNγ expression even in healthy donors (n = 14) NK. …”
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High-Affinity Anti-VISTA Antibody Protects against Sepsis by Inhibition of T Lymphocyte Apoptosis and Suppression of the Inflammatory Response by Tianzhu Tao, Lulong Bo, Teng Li, Longbao Shi, Hui Zhang, Bo Ye, Yuhai Xu, Qingqing Ma, Xiaoming Deng, Guorong Zhang

“…Engagement of VISTA receptor with MH5A mitigated CD3+ T cell apoptosis cultured from CLP mice and suppressed LPS-induced cytokine production by macrophage in vitro. …”
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Effect of Transglutaminase and Bacterial Concentrates on the Development of Functional and Technological Properties of Minced Meat by Svetlana Merenkova, Oksana Zinina, Olga Loretts, Olga Neverova, Pavel Sharaviev

“…Significant differences were noted in the changes in WBC and WHC when BCs were added to the minced meat, as dependent on the composition of the microorganism cultures. The lowest cooking losses were determined in the S-TG sample, i.e. 19.03–19.99% (p<0.05), whereas in the S-AD+TG and S-K+TG samples they reached 17.91–18.61% (p<0.05) and 18.96–19.58% (p<0.05), respectively. …”
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Mechanism of mTOR/RILP-regulated autophagic flux in increased susceptibility to myocardial ischemia-reperfusion in diabetic mice by Jiyao Zhao, Wei Shi, Yan Zheng, Junjie Wang, Muzhao Yuan, Yultuz Anwar, Yuxuan He, Haiping Ma, Jianjiang Wu

“…Inhibition of mTOR during hypoxia/reoxygenation (H/R) injury restored RILP expression and attenuated cellular injury in cardiomyocytes cultured with high glucose.ConclusionFollowing I/RI in diabetic myocardium, an increase in the CM1 subpopulation and a reduction in RILP expression result in impaired autophagic flux. …”
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Dual targeting PD-L1 and 4-1BB to overcome dendritic cell-mediated lenalidomide resistance in follicular lymphoma by Zhong Zheng, Jian-Biao Wang, Rui Sun, Nan Wang, Xiang-Qin Weng, Tian-Yuan Xu, Di Fu, Yan Feng, Peng-Peng Xu, Shu Cheng, Li Wang, Yan Zhao, Bin Qu, Chuan-Xin Huang, Wei-Li Zhao

“…PU.1 transcriptionally downregulated PD-L1 (Programmed death ligand 1) expression and upregulated 4-1BBL (4-1BB ligand) expression, increased lymphoma cell autophagy and dendritic cell maturation via PD-1/PD-L1 and 4-1BB/4-1BBL interaction. In vitro in co-culture system and in vivo in murine xenograft model, knockdown of PU.1 induced lenalidomide resistance, but sensitized FL cells to bi-specific PD-L1/4-1BB antibody or combined treatment of PD-L1 inhibitor and 4-1BB agonist. …”
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Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations by Barbara Hersant, Mounia Sid-Ahmed, Laura Braud, Maud Jourdan, Yasmine Baba-Amer, Jean-Paul Meningaud, Anne-Marie Rodriguez

“…Moreover, PRP treatment ameliorated the survival and activated the proliferation of in vitro cultured MSC and that these effects were accompanied by an alteration of the MSC energetic metabolism including oxygen consumption rate and mitochondrial ATP production. …”
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Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction by Yexin Zhang, Xiaohui He, Jiarui Li, Wentao Yang, Yinghua Cui, Shuchao Pang, Haihua Wang, Bo Yan

“…A novel deletion variant (g.106326168_70delTCT) and an SNP [g.106325757C > G (rs190825454)] were found in one 66-year-old male patient with non-ST-segment elevated AMI, but in none of controls. In cultured HEK-293 and H9c2 cells, the deletion variant significantly decreased the transcriptional activity of the ATG5 gene promoter (P<0.01). …”
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Morphokinetic Analyses of Fishing Cat–Domestic Cat Interspecies Somatic Cell Nuclear Transfer Embryos Through A Time-Lapse System by Hai-Jun Liu, Serena Jocelyn Wai Yin Oh, Nicole Liling Tay, Christina Yingyan Lim, Chia-Da Hsu, Delia Hwee Hoon Chua, Winnie Koon Lay Teo, Yuin-Han Loh, Soon Chye Ng

“…A time-lapse live embryo monitoring system provides a powerful approach to recording dynamic developmental events of cultured embryos in detail. By obtaining continuous short-interval images, blastocyst formation can be predicted and embryos can be selected. …”
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Differential Regulation of Methylation-Regulating Enzymes by Senescent Stromal Cells Drives Colorectal Cancer Cell Response to DNA-Demethylating Epi-Drugs by Khushboo Agrawal, Viswanath Das, Natálie Táborská, Ján Gurský, Petr Džubák, Marián Hajdúch

“…In contrast, the conditioned medium from senescent fibroblasts that upregulated NF-κB activity altered deoxycytidine kinase levels in drug-untreated CRC cells and abrogated DAC effect on degradation of DNA methyltransferase 1. Similar to 2D cultures, senescent fibroblasts increased DNA demethylation of CRC cells in coculture spheroids, in addition to increasing the stemness of CRC cells. …”
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EXPERIENCE OF IMPLEMENTING THE DISTANCE E-COURSE “STATISTICAL METHODS IN PHARMACY” IN THE MATHEMATICAL TRAINING OF FUTURE PHARMACEUTICAL SPECIALISTS by Iryna Zhovtonizhko, Maryna Nessonova, Mariana Barannyk, Nadiia Sheykina, Olga Marchenko

“…It is concluded, that the implementation of distance learning technology in the practice of Ukrainian higher pharmaceutical and medical education is necessary not only due to the current conditions that make it impossible to organize full-fledged offline education but also because of the efficiency of these technologies, primarily for organizing independent students’ work, that ensures the development of learners’ autonomy, contributes to the development of information culture, general digital competence, and a range of soft skills, including communication, interaction, collaboration in a digital environment, and the rational use of study time. …”
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Direct engulfment of synapses by overactivated microglia due to cadmium exposure and the protective role of Nrf2 by Siyao Li, Mingdan You, Chengjie Chen, Jingqi Fu, Yuanyuan Xu, Jingbo Pi, Yi Wang

“…Finally, we developed co-culture systems of either Nrf2-KD or Scramble microglia and primary neurons or HT22 neurons to study the effects of Nrf2-regulated microglial activation on synaptic damages induced by Cd. …”
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Process Optimization and Antioxidant Activity Analysis of White Kidney Bean Polypeptides Prepared by Composite Strain Fermentation by Sinan LI, Meng WANG, Yu AN, Qing WANG, Kaiyuan XU, Jia WANG, Zhihui ZHANG, Xi WANG, Ying WANG, Lu ZHANG

“…In order to investigate the optimal process conditions and in vitro antioxidant activity of white kidney bean polypeptides (WKBPs) from complex strain fermentation, this study used Bacillus subtilis and Aspergillus oryzae as starter culture, and optimized the process of preparing WKBPs through single factor and response surface experiments. …”
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High quality genome assembly of the brown hare (Lepus europaeus) with chromosome-level scaffolding by Michell, Craig, Collins, Joanna, Laine, Pia K., Fekete, Zsófia, Tapanainen, Riikka, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…The chromosomal assembly of the genome also demonstrates that the cell line has not acquired karyotypic changes during culture.…”
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Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway by Shubin Wang, Xiangjun Liu, Lu Xu, Jinyi Lang, Dengqun Liu, Dengqun Liu

“…The gross images of colons, general health, histology, barrier integrity, and qRT-PCR were performed. Meanwhile, cell culture and colonic organoids were used to examine the influence of iron depletion in vitro. …”
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