The contribution of leaders' and managers' attributes, values, principles, and behaviours to the sustainable implementation of Lean in healthcare: A realist review protocol [versio... by Seán Paul Teeling, Anne Marie Keown, Martin McNamara

“…Effective implementation of Lean requires robust leadership to sustain improvements and foster a culture of continuous improvement. However, the attributes, values, principles, and behaviours of effective Lean leaders in healthcare remain underexplored. …”
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Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study by Sangeeta Umesh Nayak, Anushka Kumari, Valliammai Rajendran, Vijendra Pal Singh, Ashwini Hegde, Keshava K. Pai

“…The blood agar plates were kept approximately 12 inches from the patient’s mouth. The microbial culture was analyzed. The colony-forming unit (CFU) counting in all three groups was assessed using one-way ANOVA test to compare among the groups (p value <0.001). …”
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Fipronil prevents transmission of Lyme disease spirochetes by Radek Šíma, Adéla Palusová, Tereza Hatalová, Luise Robbertse, Petra Berková, Martin Moos, Petr Kopáček, Veronika Urbanová, Jan Perner, Ala Tabor

“…No spirochetes were detected in any organ of mice exposed to fipronil-treated ticks, while 9 out of 10 control mice, exposed to non-treated infectious ticks, displayed Borrelia infection. The in vitro co-culture experiments confirmed that fipronil had no direct effect on Borrelia viability, indicating a tick-directed effect. …”
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Decoding Urinary Tract Infection Trends: A 5-Year Snapshot from Central Portugal by Francisco Rodrigues, Patrícia Coelho, Sónia Mateus, Armando Caseiro, Hatem Eideh, Teresa Gonçalves, Miguel Castelo Branco

“…Methods: A total of 6161 positive urine cultures collected over five years were examined, with particular emphasis on 2019 due to a peak in infection rates. …”
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Long-term storage of C-141 reference strain of melioidosis agent (<i>Burkholderia pseudomallei</i>) by E. A. Artemeva, L. A. Melnikova, A. P. Rodionov

“…The strain was refreshed by passaging in golden hamsters and Burkholderia culture was isolated and freeze-dried. Burkholderia pseudomallei С-141 freeze-driedstrain was tested for quality parameters, records were made and the strain was deposited.…”
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Hypoxia lowers cell carbon and nitrogen content and accelerates sinking of a marine diatom Thalassiosira pseudonana by Bokun Chen, Bokun Chen, Hui Song, Hui Song, Ge Xu, Hongli Ji, Hongli Ji, Xue Yang, Xue Yang, Gang Li

“…To uncover the underlying mechanisms, the marine model diatom Thalassiosira pseudonana was cultured under three dissolved O2 levels (8.0 mg L-1, ambient O2; 4.0 mg L-1, low O2; and 1.3 mg L-1, hypoxia) to compare its growth, cell composition, and physiology between the light and dark periods. …”
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NF-κB Inhibition after Cecal Ligation and Puncture Reduces Sepsis-Associated Lung Injury without Altering Bacterial Host Defense by Hui Li, Wei Han, Vasilly Polosukhin, Fiona E. Yull, Brahm H. Segal, Can-Mao Xie, Timothy S. Blackwell

“…Treatment with the NF-κB inhibitor did not affect the ability of cultured macrophages to phagocytose bacteria and did not alter bacterial colony counts in blood, lung tissue, or peritoneal fluid at 24 hours after CLP. …”
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Influence of Adipose Tissue-Derived Stem Cells on the Burn Wound Healing Process by Claudio Luciano Franck, Alexandra Cristina Senegaglia, Lidiane Maria Boldrini Leite, Sérgio Adriane Bezerra de Moura, Nathália Forti Francisco, Jurandir Marcondes Ribas Filho

“…Initially, intra-abdominal adipose tissue was resected from a single donor Wistar rat that was not part of any of the subsequent groups to obtain ADSCs by isolation and cell culture. Burns were made in the left lateral abdominal region of Wistar rats by contact with a square ceramic paper with a 484 mm2 area heated to 100°C for 30 seconds. …”
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Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine by Seyed Muhammad Hamid Malekpour, Moj Khaleghi, Alireza Akhtarpour

“…Twenty strains were isolated from the soil of Langar, Kerman, Iran, with characteristics of actinobacteria (gram-positive filamentous or rod-shaped bacteria and powdery consistency colonies that stick firmly to agar surface). All strains were cultured in a medium with L-tyrosine to assess tyrosinase production. …”
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Carrageenan-Induced Colonic Inflammation Is Reduced in Bcl10 Null Mice and Increased in IL-10-Deficient Mice by Sumit Bhattacharyya, Liquan Xue, Suzanne Devkota, Eugene Chang, Stephan Morris, Joanne K. Tobacman

“…IL-10-deficient mice exposed to carrageenan in a germ-free environment showed an increase in activation of the canonical pathway of NF-κB (RelA) activation, but without increase in RelB or phospho-Bcl10, and exogenous IL-10 inhibited only the canonical pathway of NF-κB activation in cultured colonic cells. These findings demonstrate a Bcl10 requirement for maximum development of carrageenan-induced inflammation and lack of complete suppression by IL-10 of carrageenan-induced inflammation.…”
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Conditioned Medium from Adipose-Derived Stem Cell Inhibits Jurkat Cell Proliferation through TGF-β1 and p38/MAPK Pathway by Xiuxia Wang, Yinmin Wang, Xianyu Zhou, Fei Liu

“…The transcript abundance of TGF-β1, as well as that of insulin-like growth factor binding protein 3 (IGF-BP3), was evaluated using qRT-PCR on Jurkat cells cultured in ADSC-CM for 24 hours. The proliferation of the Jurkat cells was assessed using cell cycle assay. …”
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The outcomes of recent patient safety education interventions for trainee physicians and medical students: a systematic review by Charles Vincent, Nick Sevdalis, Sonal Arora, Paul Baker, Matthew A Kirkman, Maria Ahmed

“…Availability of expert faculty, competing curricular/service demands and institutional culture were important factors affecting implementation.Conclusions There is an increasing trend for developing educational interventions in patient safety delivered to trainees/residents and medical students. …”
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Effects of Placenta-Derived Mesenchymal Stem Cells on the Particulate Matter-Induced Damages in Human Middle Ear Epithelial Cells by So Young Kim, Seung Ha Oh, Jun Ho Lee, Myung-Whan Suh, Moo Kyun Park

“…Cells were harvested on days 0, 1, and 4, and the expression of the inflammatory genes TNFα, COX2, IL1β, IL6, and MUC5B in HMEECs and anti-inflammatory genes PTGES, TGFβ, and VEGF in PL-MSCs was examined by qRT-PCR. The culture media were collected to measure the secreted PGE2 level using an enzyme-linked immunosorbent assay. …”
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Muscarinic cannabinoid suppression of excitation, a novel form of coincidence detection by Michaela Dvorakova, Ken Mackie, Alex Straiker

“…These receptors are implicated in several forms of synaptic plasticity – depolarization-induced suppression of excitation (DSE), metabotropic suppression of excitation (MSE), long term depression (LTD) and activation-dependent desensitization. Cultured autaptic hippocampal neurons express all of these, illustrating the rich functional and temporal heterogeneity of CB1 at a single set of synapses. …”
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Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells by Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco

“…Dental pulp stem cells (DPSCs), derived from the premolar tooth, were conditioned for 21 days with oral plaque samples and tested for their clonogenic ability. Cultures were also induced to differentiate towards the osteogenic lineage, and ALP and osteocalcin gene expression levels were evaluated by real-time qPCR. …”
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Rothia mucilaginosa Meningitis in a Child with Myelodysplastic Syndromes by Fumihiro Ochi, Ryota Nakamura, Reiji Miyawaki, Kyoko Moritani, Shinobu Murakami, Hisamichi Tauchi

“…We retrospectively reviewed the antimicrobial susceptibility of all R. mucilaginosa isolates (n = 5) from blood or cerebrospinal fluid cultures at our hospital. The MIC of VCM was <0.5 μg/mL for all strains, whereas the MIC of DAP was ≥2 μg/mL for all strains. …”
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Testing of diagnostic test-systems for detection of antibodies to foot-and-mouth disease virus structural proteins with enzyme-linked immunosorbent assay for their serotype specifi... by N. N. Lugovskaya, E. A. Silanteva, T. V. Okovytaya, A. A. Kharitonova, Y. M. Gochmuradov, E. A. Razgulyaeva, O. O. Budina, E. A. Yasneva

“…Complex tests for FMDV using different diagnostic methods such as ELISA with standard and reference test-systems and/or virus neutralization test in cell culture are to be considered as the best option.…”
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TRAIL and Ceruloplasmin Inverse Correlation as a Representative Crosstalk between Inflammation and Oxidative Stress by Veronica Tisato, Stefania Gallo, Elisabetta Melloni, Claudio Celeghini, Angelina Passaro, Giorgio Zauli, Paola Secchiero, Carlo Bergamini, Alessandro Trentini, Gloria Bonaccorsi, Giuseppe Valacchi, Giovanni Zuliani, Carlo Cervellati

“…After stratification for high-sensitivity C-reactive protein levels, the inverse association between TRAIL and ceruloplasmin levels remained strong and significant (r=−0.508, p<0.001, R2=0.260) only in the presence of inflammation, confirming the role of inflammation as emerged in in vitro experiments where recombinant TRAIL decreased ceruloplasmin expression levels in TNF-treated PBMC cultures. Conclusion. The results indicated that in an inflammatory milieu, TRAIL downregulates ceruloplasmin expression, highlighting a signaling axis involving TRAIL and ceruloplasmin that are linked via inflammation and providing important insights with potential clinical implications.…”
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Optimization of Fermentation Conditions for Increasing Erucamide Content in <i>Bacillus megaterium</i> Using Several Accelerants by Hao Zhao, Yudan Xie, Zhu Li, Longfeng Wei, Renli Ai

“…Univariate and orthogonal tests showed that 0.3% Tween 80, 0.004% Ca²⁺, 0.04% colza oil, and 0.02% chloroform were the best regulation conditions for erucamide production of the L2 strain, and erucamide content reached 1.778 mg/L, which was 32.59% higher than the blank group and 60.26% higher than before fermentation culture. The mechanism of membrane metabolism in the L2 strain was further investigated, and our data suggested that the conductivity, nucleic acid and protein content, and β-galactosidase activity of L2 were increased significantly after treatment with accelerants, indicating that the accelerants changed the cell membrane permeability of the L2 strain but did not harm or kill the bacteria. …”
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Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling by Zhengjun Peng, Lu Liu, Wenyu Zhang, Xi Wei

“…Alternation in culture environment due to cell-cell communications can rejuvenate the biological activity of aged/differentiated cells and stimulate the expression of pluripotency markers. …”
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