Cytotoxic Activity of Bisphosphonic Derivatives Obtained by the Michaelis–Arbuzov or the Pudovik Reaction by Zsuzsanna Szalai, Janka Bednárik, Boldizsár Szigfrid Tóth, Angéla Takács, Szilárd Tekula, László Kőhidai, Konstantin Karaghiosoff, László Drahos, György Keglevich

“…<b>Conclusions:</b> The new families of compounds synthesized by our novel approaches may be of practical importance due to the significant cytotoxic activity on the cell cultures investigated. Compounds lacking hydroxy groups showed anti-myeloma activity or limited effect on pancreatic cancer (PANC-1) cells unless substituted with para-trifluoromethyl group. …”
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Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice by Shailendra P. Singh, Lars Bellner, Luca Vanella, Jian Cao, John R. Falck, Attallah Kappas, Nader G. Abraham

“…We postulate that EETs attenuate adiposity by stimulating mitochondrial function and induction of HO-1 via activation of PGC-1α in adipose and hepatic tissue. Methods. Cultured murine adipocytes and mice fed a high fat (HF) diet were used to assess the functional relationship among EETs, PGC-1α, HO-1, and mitochondrial signaling using an EET-agonist (EET-A) and PGC-1α-deficient cells and mice using lentiviral PGC-1α(sh). …”
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Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…The warmed embryos which were frozen at 8-cell stage were cultured in vitro until the blastocyst stage and were transferred into recipients. …”
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Profiling the response of individual gut microbes to free fatty acids (FFAs) found in human milk by Megan E. Waller, Alyssa Gutierrez, Taylor D. Ticer, Janiece S. Glover, John E. Baatz, Carol L. Wagner, Melinda A. Engevik, Katherine E. Chetta

“…Select species of commensal and pathobiont genera (Bifidobacterium, Lactobacillus, Streptococcus, Staphylococcus, Enterococcus, Acinetobacter, Pseudomonas, Escherichia, and Klebsiella) were cultured with FFAs. The growth of all commensals, except for L. johnsonii, was significantly inhibited by the highest concentration (1 %) of all FFAs. …”
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Promoting ER stress in a plasmacytoid dendritic cell line drives fibroblast activation by Beatriz H. Ferreira, Inês S. Silva, Andreia Mendes, Fátima Leite-Pinheiro, Adrienne W. Paton, James C. Paton, Iola F. Duarte, Philippe Pierre, Catarina R. Almeida

“…More specifically, IMR-90 fibroblasts were co-cultured with CAL-1, a pDC cell line. ER stress was then induced by the bacterial toxin SubAB. …”
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Artemisia capillaris with two novel active compounds, Kawarayomogin I and II, inhibits HYBID (KIAA1199) expression as well as hyaluronic acid degradation by Kazal Boron Biswas, Yuka Kawai, Satoshi Nakagawa, Kyoko Kanai, Hiroyuki Kojima, Teruaki Masutani, Masayoshi Oyama, Arunasiri Iddamalgoda, Kotaro Sakamoto

“…The molecular size distribution of HA was evaluated by incubating fluorescein isothiocyanate (FITC)-labeled large HA (1200–1600 kDa) in NHDF for certain time followed by measuring different sizes of FITC-labeled HA in the cultured medium by HPLC. Among 380 plant extracts, we found that Artemisia capillaris flower extract (ACFE) was the most effective agent in both suppressing HYBID expression as well as protecting large HA from degradation. …”
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Optimizing winged-bean meal through oven and autoclave heating as viable alternative for plant-based aquafeed by Shobrina Silmi Qori Tartila, Tholibah Mujtahidah, Muh. Azril, Anjali Apta Pramudita, Lusi Septiani, Bagas Putra Satria, Ignatius Abel Cristanto, Muhammad Abizar Sakti Oktavian, Sultan Jibran Risqulloh, Santika Dewi Astuti

“…Data were also compared to the nutrient requirements of several cultured freshwater fish in Indonesia. Heating treatments improved in vitro trypsin activity (1300 units/O and 1135 units/A vs. 835 units) but reduced protein (29.05±1.96%/A and 31.73±1.52%/O vs. 35.17±0.38%) and moisture contents. …”
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Transcriptional Regulation of Δ6-Desaturase by Peroxisome Proliferative-Activated Receptor δ Agonist in Human Pancreatic Cancer Cells: Role of MEK/ERK1/2 Pathway by Maryam Darabi, Shima Byagowi, Shabnam Fayezi, Masoud Darabi, Shahab Mirshahvaladi, Mehdi Sahmani

“…The aim of the present study was to investigate the effect of peroxisome proliferative-activated receptor δ (PPARδ) agonist and MEK/ERK1/2-dependent pathway on the expression of Δ6D in human pancreatic carcinoma cell line PANC-1. PANC-1 cells cultured in RPMI-1640 were exposed to the commonly used ERK1/2 pathway inhibitor PD98059 and PPARδ agonist GW0742. …”
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Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells by Jiangyi Wang, Guoqing Li, Lei Hu, Fei Yan, Bin Zhao, Xiaoshan Wu, Chunmei Zhang, Jinsong Wang, Juan Du, Songlin Wang

“…Human DPSCs were isolated and cultured in osteogenic induction medium with or without RA or BMS 493, an inverse agonist of the pan-retinoic acid receptors (pan-RARs). …”
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Human Capital and Social Capacity of the Regional Space of Arctic Karelia by Alexander D. Volkov, Aleksandr O. Averyanov, Natalia A. Roslyakova, Polina A. Golomydko

“…The first task is to study the territorial conditions of human capital reproduction related to its social aspect: health care system, education system, culture, leisure and entertainment. The second task is to assess the possibility of applying the concept of social capacity of space to study the social component of the conditions of human capital reproduction. …”
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Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation by Najmeh Anbiaee, Mohammad Taghi Bahreyni Toossi, Hosein Azimian, Zoha Sahebnasagh, Sepideh Abdollahi Dehkordi, Majid Kianmehr, samaneh soudmand salarabadi, Maryam Najafi Amiri

“…The protective effects of the green tea polyphenol on Bcl-2 and Bax genes expression levels were evaluated in cultured lymphocytes obtained from treated cells (24 h and 1 h before and 1 h after exposure to ionizing radiation) and non-treated cells (Receiving X-radiation and non-receiving green tea).Results: The results of this investigation demonstrated Bax/Bcl-2 ratio, as an index of the radiation sensitivity, was significantly decreased in groups which had been received dose of 2 Gy in treated cells groups of 1 h before and 1 h after X-radiation (p<0.05).Conclusion: The results indicated different effects of green tea on low and high doses of X-radiation. …”
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Comparison evaluation of bacterial DNA extraction methods for improved molecular diagnostic accuracy of sepsis-causing pathogens in clinical whole blood samples by Byungjoon Na, Junghun Park, Sojin Park, Eunseon Park, Jimin Jang, Yu-Hee Kim, Jinyeop Lee, Hae-Sun Chung

“…Current diagnostic methods such as blood cultures are time-consuming, whereas molecular diagnostic techniques represent a promising alternative for faster pathogen detection. …”
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The Efficacy of Single-Application NPWTi-d for the Salvage of Infected Breast Prostheses: A Multi-Center Study by Jason C. Llaneras, MD, Robert Craig Clark, MD, Lauren Antognoli, MD, Emily Finkelstein, MD, Luci Hulsman, BS, Luther Holton, MD, Devinder Singh, MD, R. Jason VonderHaar, MD, Risal Djohan, MD, Aladdin H. Hassanein, MD, Chris M. Reid, MD

“…Methicillin-resistant organisms were cultured in 15% of cases. NPWTi-d was applied for an average of 61 hours with dwell times of 18 minutes every 3 hours, using institution-specific instillates. …”
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Clinical Predictors of Pseudomonas aeruginosa Bacteremia in Emergency Department by Yongsoon Choi, Jin Hui Paik, Ji Hye Kim, Seung Baik Han, Areum Durey

“…This was a single-center retrospective case-control study to investigate the clinical predictors of patients diagnosed as Pseudomonas bacteremia in the emergency department (ED) from June 2012 to December 2016. Patients with blood culture positive for Escherichia coli in the same period were chosen as the control group, and type of infection was matched for each patient. …”
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A backwater technology for enhancing pollutants discharge from aquaculture tanks in a Litopenaeus vannamei recirculating aquaculture system: Performance and control models by Jianping Xu, Yishuai Du, Jiawei Zhang, Fudi Chen, Li Zhou, Tianlong Qiu, Jianming Sun

“…The RAS has ten aquaculture tanks, with an total effective culture volume of 420 m3 and a stocking density of 800 shrimp/m3. …”
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Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices by Veronica Folliero, Gianluigi Franci, Federica Dell’Annunziata, Rosa Giugliano, Francesco Foglia, Rossella Sperlongano, Anna De Filippis, Emiliana Finamore, Massimiliano Galdiero

“…Biofilm-forming abilities were assessed using the tissue culture plate (TCP) method. Among the 94 MDs isolated strains, 42.7% were Gram-positive, 40.3% Gram-negative, and 17% Candida species. …”
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Evaluation of a Commercial Multiplex Real-Time PCR with Melting Curve Analysis for the Detection of <i>Mycobacterium tuberculosis</i> Complex and Five Nontuberculous Mycobacterial... by Keun Ju Kim, Yunhee Chang, Seung Gyu Yun, Myung-Hyun Nam, Yunjung Cho

“…A total of 91 acid-fast bacillus culture-positive samples, comprising 36 MTBC and 55 NTM isolates, were collected from the Korea University Anam Hospital. …”
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Lettuce seedlings quality: The effects of white and blue light exposure by Luković Kristina, Adžić Slađan, Zečević Veselinka, Đurić Nenad, Perišić Vladimir, Gavrilović Bojana, Rakonjac Aleksandra

“…In the phase of three leaves, plants were placed in plant growth chambers under multispectral white (W) LEDs and monochromatic blue (B) LEDs (three containers per LED and genotype), while three containers were kept in the greenhouse and served as control. The plants were cultured in plant growth chambers for 25 days under a 9h/15h and 10h/14h (light/dark) photoperiod, to simulate outdoor conditions. …”
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Huntington’s disease modeling on HEK293 cell line by D. V. Sharipova, T. B. Malankhanova, A. A. Malakhova

“…Therefore, one of the important tasks is generation of an in vitro model system of Huntington’s disease based on human cell cultures. The new genome editing approaches, such as CRISPR/Cas9, allow us to generate isogenic cell lines that can be useful for drug screening and studying mechanisms of molecular and cellular events triggered by certain mutation on an equal genetic background. …”
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Lyophilized and Oven-Dried <i>Manilkara zapota</i> Extracts: Characterization and <i>In Vitro</i>, <i>In Vivo</i>, and <i>In Silico</i> Analyses by María Fernanda Rivas-Gastélum, Patricia Ariadna Galindo-Castillo, Juan Esparza-Sánchez, Miriam Irene Jiménez-Pérez, Yocanxóchitl Perfecto-Avalos, Luis Eduardo Garcia-Amezquita, Diego E. Navarro-López, Edgar R. López-Mena, Eugenio Sánchez-Arreola, Juan Pablo Tamayo-Martínez, Humberto L. Mendoza-Figueroa, María Magdalena Crosby-Galván, Elsa Margarita Crosby-Galván, Jorge L. Mejía-Méndez, Angélica Lizeth Sánchez-López

“…<i>In silico</i> evaluation unveiled that polar compounds in <i>M. zapota</i> fruits possess a high binding affinity towards the DNA gyrase B of the cultured strains. This study expands the scientific evidence regarding the influence of distinct extraction methods on the nutritional and nutraceutical content of native fruits and the importance of considering additional approaches to enhance their bioactivities.…”
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