Canadian Public Health Laboratory Network laboratory Guidelines for the Use of Serological Tests (excluding point-of-care tests) for the Diagnosis of Syphilis in Canada by Paul N Levett, Kevin Fonseca, Raymond SW Tsang, Kamran Kadkhoda, Bouchra Serhir, Sandra M Radons, Muhammad Morshed

“…Because this organism cannot be cultured, serology is used as the principal diagnostic method. …”
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Konsep Pendidikan Islam Dalam Penguatan Moderasi Beragama by Yesi Arikarani, Zainal Azman, Siti Aisyah, Fadillah Putri Ansyah, Tri Dinigrat Zakia Kirti

“…In social life, there are certainly many differences that make us have to understand and respect the differences that exist, as is the case in Indonesia, which has a variety of ethnicities, religions, and cultures, so everyone must have an attitude of tolerance towards these things. …”
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THE ROLE OF CALDERA GEOPARK IN TOURISM DEVELOPMENT OF LAKE TOBA SUPER PRIORITY DESTINATIONS, INDONESIA by Bejo MULYADI, Sirojuzilam SIROJUZILAM, Suwardi LUBIS, Agus PURWOKO

“…Meanwhile, attention to environmental sustainability, local culture, and community participation was important for managing the UNESCO Global Geopark.…”
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Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants by Kishan Raj, Anup Singh, Tapas Chowdhury, S Gupta, Ravindra Soni

“…The results concluded that the AMB-CD-1 culture inoculated straw had a low level of cellulose and hemicellulose, indicating these particles’ degradation. …”
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ANKRD22 participates in the proinflammatory activities of macrophages in the colon cancer tumor microenvironment by Xiaoying Wang, Keqing Yang, Bin Yang, Rui Wang, Yongliang Zhu, Tianhui Pan

“…A significant decrease in ANKRD22 expression in macrophages cocultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues was observed. …”
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Hybrid Repair Combined with Fresh Arterial Allograft Extra-Anatomical Reconstruction: The Treatment of Infrarenal Abdominal Aneurysm above an Aortobifemoral Bypass Complicated by a... by Robert Novotny, Tomas Marada, Jiri Novotny, Jakub Kristek, Jaroslav Chlupac, Michal Kudla, Kvetoslav Lipar, Jiri Mendl, Jiri Fronek, Libor Janousek

“…Postprocedurally, the hospital stay was uneventful. The left groin PSA cultures came positive for Staphylococcus epidermidis and Corynebacterium tuberculostearicum, both sensitive to vancomycin and rifampicin. …”
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Comparative analysis of combined methylene blue photodynamic therapy and doxorubicin treatment of oral squamous cell carcinoma cell line: In vitro study on apoptosis by Shayan Momeni Goujani, Maryam Koopaie, Fateme Hamta Safarian, Neda Hakimiha, Shima Younespour

“…Materials and methods: The human oral epidermal carcinoma cell line (KB cell line, NCBI Code: C152) was cultured in Dulbecco's modified Eagle's medium. Following at least 24 hours of incubation, the OSCC cells were distributed into six groups, with groups 1–3 and 5 performed in the dark to prevent any light interference. 1: control group; 2: treated with 3.2 μg/mL methylene blue; 3: exposed to various concentrations of doxorubicin; 4: PDT group (methylene blue + 660 nm light); 5: treated with both doxorubicin and methylene blue; and finally, 6: treated with PDT (methylene blue + 660 nm light) in conjunction with doxorubicin. …”
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Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection by Yong Li, Chuanlong Zhu, Faxi Wang, Tiantian Zhu, Jun Li, Shufeng Liu, Fei Xiao

“…It has been recently reported that SART1 mediates antiviral effects of IFN-α in the hepatitis C virus (HCV) cell culture model. In this study, we investigated the role of SART1 in antiviral activity of IFN-α against hepatitis B virus (HBV) using blood and liver biopsy samples from chronic hepatitis B patients treated with pegylated IFN-α and HepG2 cells transfected with cloned HBV DNA. …”
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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…However, long-term in vitro culture results in loss of MSC stemness. The inflammation that occurs at stem cell transplant sites (such as that resulting from TNF-α) is a contributing factor for stem cell treatment failure. …”
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RAISE OF HIGH FROST-RESISTANT AGROPYRON–TRITICUM HYBRIDS by E. P. Razmakhnin, T. M. Razmakhnina, V. E. Kozlov, E. I. Gordeeva, N. P. Goncharov, G. Y. Galitsyn, S. G. Veprev, V. M. Chekurov

“…A biotechnological approach to obtaining pure androgenous wheatgrass lines via anther culture and an efficient method for rapid analysis of frost resistance in winter wheat, wheatgrass and other crops were developed. …”
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“It’s Traumatic for All of Us”: A Qualitative Analysis of Providers Caring for Seriously Ill Veterans With Surgical Conditions by Adela Wu, MD, Sarah E. Bradley, PhD, MPH, CPH, C. Ann Vitous, MA, MPH, M. Andrew Millis, MD, MPH, Pasithorn A. Suwanabol, MD, MS

“…Providers described clinical encounters that generated moral distress while caring for seriously ill patients with surgical conditions: (1) difficulties in conflict resolution with and among patients and families; (2) specific types of patients or situations; (3) systemic factors hindering appropriate end-of-life care; (4) surgical culture and expectations of the surgeon’s role. Conclusions:. …”
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Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth and the Orbicularis Oris Muscle: How Do They Behave When Exposed to a Proinflammatory Stimulus? by Alessander Leyendecker Junior, Carla Cristina Gomes Pinheiro, Daniela Yukie Sakai Tanikawa, José Ricardo Muniz Ferreira, Mariane Tami Amano, Daniela Franco Bueno

“…The results demonstrated that the proliferation of SHED and OOMDSCs was inhibited by the addition of IFN-γ to their culture medium and that treatment with IFN-γ at higher concentrations resulted in a greater inhibition of the proliferation of these cells than treatment with IFN-γ at lower concentrations. …”
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Body image and sexual life satisfaction in women considering female genital cosmetic surgery in Iran by Setareh Derakhshanpour, Fatemeh Bagheri, Fatemeh Rahmanian, Seyede Zahra Jafari, Zahra YazdanPanahi

“…Abstract Background Further attention to genital organs has become a concern for women in line with changes in society’s culture. In recent years, the number of surgeries has grown increasingly. …”
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Effect of Co-fermentation with Streptococcus salivarius subsp. thermophilus and Bifidobacterium animalis subsp. lactis on Improving the Quality of Fermented Milk by LIU Zhizhong, LIU Liwei, LIU Zhigang, CHEN Haili, CUI Hong, YANG Bo, ZHANG Jiaqi, WU Rigeng, YAO Kai

“…Additionally, the VBC in the co-fermented milk was significantly higher than that in the single-strain fermented milk throughout the storage period, indicating that the co-fermentation could significantly enhance the storage stability and probiotic functions of the product. The use of co-cultures markedly improved the rheological and texture characteristics of fermented milk, particularly viscosity and WHC. …”
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Motif distribution and DNA methylation underlie distinct Cdx2 binding during development and homeostasis by Alireza Lorzadeh, George Ye, Sweta Sharma, Unmesh Jadhav

“…Induced DNA methylation in adult mouse epithelium or cultured cells recruits Cdx2 to developmental targets, promoting corecruitment of partner transcription factors. …”
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Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province by Ali D. Marhash, Zainab N. Nabat, Nawras A. Abbas

“…GBS was detected via culture methods and was confirmed by PCR with primers employed for the detection of atr gene (housekeeping gene). …”
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V-ATPase in glioma stem cells: a novel metabolic vulnerability by Alessandra Maria Storaci, Irene Bertolini, Cristina Martelli, Giorgia De Turris, Nadia Mansour, Mariacristina Crosti, Maria Rosaria De Filippo, Luisa Ottobrini, Luca Valenti, Elisa Polledri, Silvia Fustinoni, Manuela Caroli, Claudia Fanizzi, Silvano Bosari, Stefano Ferrero, Giorgia Zadra, Valentina Vaira

“…Methods V-ATPase activity in GSC cultures was modulated using Bafilomycin A1 (BafA1) and cell viability and metabolic traits were analyzed using live assays. …”
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Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast Strains <i>Kluyveromyces marxianus</i> JMY140K and <i>Metschnikowia reukaufii</i> JMY075 by Ting-Ting Fan, Chao Chen, Du-Wen Zeng, Feng-Lou Wang, Zhao-Xian Xu, Ming-Jie Jin, Yue Zou, Jun Li, Xin-Qing Zhao

“…External stress conditions significantly influenced the GABA production of these two yeast strains. The culture filtrate of <i>K. marxianus</i> JMY140K also showed promising activities in human skin cells. …”
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Adaptation of the nutrition care process for metabolic diseases in the Mexican population by Magdalena Sevilla-González, Magdalena Sevilla-González, Magdalena Sevilla-González, Ailema González-Ortiz, Ailema González-Ortiz, María Victoria Landa-Anell, Marco A. Melgarejo-Hernández, Marco A. Melgarejo-Hernández, Ana Teresa Arias-Marroquín, Fabiola Mabel Del Razo-Olvera, Fabiola Mabel Del Razo-Olvera, Berenice Monserrat Román-Calleja, Ana Victoria Monreal-Lugo, Angélica J. Martin-Vences, Karime Haua-Navarro, Angeles Espinosa-Cuevas

“…Given the high prevalence of metabolic diseases in Mexican population, there is a critical need for tailored nutrition care strategies.ObjectiveWe aim to describe the adaptation of the NCP to manage metabolic diseases in Mexican individuals.MethodsOur adaptation included a comprehensive literature review of clinical nutrition guidelines, by a structured consultation with experts to ensure clinical setting-specific and culturally appropriate modifications. A team of registered dietitians from two tier 3 hospitals, each with over five years of experience in metabolic disease management, customized the NCP’s four core steps—assessment, diagnosis, intervention, and monitoring—to meet the specific needs of the Mexican population.ResultsWe adapted the NCP to manage five common metabolic disorders: obesity, type 2 diabetes, kidney disease, metabolic dysfunction-associated steatotic liver disease, and dyslipidemia. …”
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Comparative performance of tuberculin and defined-antigen cocktails for detecting bovine tuberculosis in BCG-vaccinated cattle in natural settings by Abebe Fromsa, Andrew J. K. Conlan, Sreenidhi Srinivasan, Balako Gumi, Wegene Bedada, Miserach Zeleke, Dawit Worku, Matios Lakew, Biniam Tadesse, Berecha Bayissa, Asegedech Sirak, Musse Girma Abdela, Getnet Abie Mekonnen, Tesfaye Chibssa, Maroudam Veerasami, Gareth J. Jones, H. Martin Vordermeier, Nick Juleff, James L. N. Wood, Gobena Ameni, Vivek Kapur

“…After exposure, the DIVA skin, DIVA IGRA, and comparative cervical tuberculin (CCT) tests had comparable sensitivities of 46% (95% CI 36, 56), 45% (95% CI 35, 55), and 47 (95% CI 37, 57), respectively, when assessed against animals positive by M. bovis culture PCR. The results suggest that test-and-slaughter control strategies using tests with low sensitivity are not expected to be effective in controlling bTB in high-prevalence herds, and highlight an urgent need to improve the sensitivity of diagnostic tests for bTB in these settings.…”
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