Improved production strain maintenance technique for Burkholderia mallei 5584 (Master seed) used for glander diagnostic agent production by E. A. Artemeva, L. A. Melnikova, L. I. Zaynullin, N. K. Bukova

“…This technique allows for the strain sub-culturing on sensitive models once every 5 years, which is more expedient and safe from an economic and biological point of view. …”
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Examining Emphysematous Pyelonephritis: A Case Study on Diagnosis and Outcomes by Mahnoor Mahnoor, Syeda Aina Ali, Saira Nasir, Moiz Azmat, Hafiz Muhammad Umer Farooqi

“…Following findings from ultrasound, she was advised to undergo computed tomographic (CT) scans that reveal air-filled hypodense areas at the upper and mid pole of the right kidney and in the renal pelvis of the right ureter, which confirms the class I EPN in the patient. Urine culture identifies <i>Escherica coli</i> as the causative agent for EPN. …”
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Effect of Compression Loading on Human Nucleus Pulposus-Derived Mesenchymal Stem Cells by Hang Liang, Sheng Chen, Donghua Huang, Xiangyu Deng, Kaige Ma, Zengwu Shao

“…Human NP-MSCs were isolated from patients undergoing lumbar discectomy for IVD degeneration and were identified by immunophenotypes and multilineage differentiation. Then, cells were cultured in the compression apparatus at 1.0 MPa for different times (0 h, 24 h, 36 h, and 48 h). …”
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High Efficiency Direct Shoot Organogenesis from Leaf Segments of Aerva lanata (L.) Juss. Ex Schult by Using Thidiazuron by K. Varutharaju, C. Soundar Raju, C. Thilip, A. Aslam, A. Shajahan

“…Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.25–2.0 mg L−1 thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organogenesis (23.6 ± 0.16) was obtained on medium containing 1.0 mg L−1 TDZ. …”
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Establishment of a Virus-Induced Gene Silencing System in <i>Abelmoschus manihot</i> L. by Ting Zhang, Jiaqi Hou, Hongtao Chu, Pengyu Guo, Qianzi Sang, Zhongxu Liu, Li Cao

“…The results demonstrated that administering two injections—the first on the day of complete cotyledon expansion and the second 14 days later—using pTRV1 and pTRV2–<i>AmPDS</i> cultures resuspended to an OD₆₀₀ of 1.0 and via the backside of the blade—led to significant photobleaching in the cotyledons 2 days after the second injection. …”
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Prenatal Identification and Molecular Characterization of Two Simultaneous De Novo Interstitial Duplications of Chromosomal Regions 7p22.1p21.1 and 15q24.1 by Sabrina C. Burn, Kali Swift, Maria Palmquist

“…Cytogenetic analysis of cultured amniocytes by conventional chromosome analysis, comparative genomic hybridization, and fluorescence in situ hybridization revealed two interstitial duplications of the chromosomal regions 7p22.1p21.1 and 15q24.1, leading to partial trisomy of 7p and 15q and karyotype 46,XY,dup(7)(p22.1-p21.1),dup (15)(q24.1). …”
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Identification and interpretive ranking of factors affecting the improvement of agility in business process management by Sadegh Hasani Moghadam, Mohammad Mahdi Mohtadi, Hossein Bazargani, Ali Taheri, Mohsen Miri

“…After that, the results showed that environmental awareness, knowledgeable and competent human resources, organizational learning dimension, contingent and appropriate structures, governance, improvisation-based strategy-making, technological infrastructure, dynamic adaptation, culture, and creative sustainability are prioritized, respectively.Originality/Value: Using soft operations research techniques, including the interpretive ranking process, in designing an agile model for managing business processes will significantly help process organizations in complex and dynamic environments to simultaneously exploit the benefits of stability in managing processes and dynamism coming from being agile.…”
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Barriers to uptake of harm reduction techniques for GBMSM who use drugs in night-clubs and sex-on-premises venues in London and the Southeast: a mixed-methods, qualitative study by Stephen Naulls, K. Oniti, J. Eccles, J. M. Stone

“…Conclusions Addressing these barriers could transform night-time venues into effective sites for harm reduction, particularly by targeting “afters” culture (the phenomenon where club-goers will return to a residential setting and continue substance use for prolonged periods ‘after’ the night-time venue closes or the event ends) and promoting safer practices. …”
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In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights by Mengwei Li, Faiz-ul Hassan, Qian Lin, Muhammad Adeel Arshad, Muhammad Uzair Akhtar, Lijuan Peng, Chengjian Yang, Xin Liang, Jiaxiang Huang

“…These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH₄ production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. …”
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Towards Safer Workplace: A Survey-Based Study on Developing a Safety Climate Model for the Indonesian Paper Industry by Nana Rahdiana, Bambang Suhardi, Retno Wulan Damayanti, Novie Susanto, Jafri Mohd Rohani

“…By fostering a proactive safety culture and addressing sector-specific risks, this model has the potential to significantly reduce workplace accidents and improve overall safety performance, marking an important advancement in industry-specific safety research.…”
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TB drug susceptibility testing in high fluoroquinolone resistance settings by F. Saluzzo, F. Masood, V. Batignani, F. Di Marco, U. Majeed, A. Ghazal, D.M. Cirillo, S. Tahseen

“…Xpert® MTB/RIF Ultra, MTB/XDR, Genotype® MTBDRplus, Genotype® MTBDRsl, culture, and phenotypic drug susceptibility testing (pDST) for relevant drugs (including bedaquiline and levofloxacin) were performed. …”
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Human Neonatal Thymus Mesenchymal Stem Cells Promote Neovascularization and Cardiac Regeneration by Shuyun Wang, Shan Huang, Lianghui Gong, Zhize Yuan, Joshua Wong, Jeffrey Lee, Ming-Sing Si

“…We also found that organoid culture of ntMSCs stimulated Shh expression. We then determined that ntMSCs were cytoprotective of neonatal rat cardiomyocytes exposed to H2O2. …”
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SHOX and sex difference in height: a hypothesis by Tsutomu Ogata, Atsushi Hattori, Maki Fukami

“…In this regard, previous studies have revealed that (1) distribution of the mean adult heights in subjects with disorders accompanied by discordance between sex chromosome complement and bioactive sex steroids and in control subjects (the British height standards) indicates that, of the ~12.5 cm of sex difference in the mean adult height, ~9 cm is accounted for by the difference in the sex chromosome complement and the remaining ~3.5 cm is explained by the dimorphism in sex steroids (primarily due to the growth-promoting effect of gonadal androgens); (2) according to the infancy-childhood-puberty growth model, the sex difference in the childhood growth function produces height differences of ~1 cm in childhood and 8–10 cm at 18–20 years of age, whereas the sex difference in the pubertal growth function yields height difference of ~4.5 cm at 18–20 years of age; and (3) SHOX expression and methylation analyses using knee cartilage tissues and cultured chondrocytes have shown lower SHOX expression levels in female samples than in male samples and methylation patterns consistent with partial spreading of X-inactivation affecting SHOX in female samples. …”
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A global framework for integrating public health into wellbeing: why a public wellbeing system is needed by László L. Lippai, László L. Lippai, Klára Tarkó, Klára Tarkó, Attila Tanyi, Attila Tanyi, Zsófia Kollányi, Zsófia Kollányi, Mária Arapovics, József Vitrai, József Vitrai

“…It underscores the importance of the need for a dedicated social subsystem where the goals and organizational culture of the organizations involved are focused on wellbeing and health promotion. …”
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Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes by Valentin Jossen, Cedric Schirmer, Dolman Mostafa Sindi, Regine Eibl, Matthias Kraume, Ralf Pörtner, Dieter Eibl

“…Nevertheless, one obstacle may be the formation of large microcarrier-cell-aggregates, which may result in mass transfer limitations and inhomogeneous distributions of stem cells in the culture broth. The dependence of microcarrier-cell-aggregate formation on impeller speed and shear stress levels was investigated for human adipose derived stromal/stem cells (hASCs) at the spinner scale by recording the Sauter mean diameter (d32) versus time. …”
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Increased Death of Peripheral Blood Mononuclear Cells after TLR4 Inhibition in Sepsis Is Not via TNF/TNF Receptor-Mediated Apoptotic Pathway by Chien-Ming Chu, Li-Chung Chiu, Chung-Chieh Yu, Li-Pang Chuang, Kuo-Chin Kao, Li-Fu Li, Huang-Pin Wu

“…Stimulated peripheral blood mononuclear cells (PBMCs) with or without lipopolysaccharides (LPS) and high-mobility group box 1 (HMGB1) were cultured with or without TLR4 inhibition using monoclonal antibodies from 20 patients with sepsis. …”
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Protocol for a qualitative study exploring haemodialysis dependent patients’ arteriovenous fistula experience, values and concerns in Sydney, Australia by Vasi Naganathan, Sarah Joy Aitken, Bethany Miriam Stavert, Susan Monaro, Lisa Tienstra

“…Introduction The experiences of patients from culturally and linguistically diverse backgrounds, with chronic mental illness, disabilities or who identify as sexual or religious minorities are under-represented in clinical research on arteriovenous fistula (AVF) for haemodialysis access. …”
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Haptoglobin Genotype-Dependent Anti-Inflammatory Signaling in CD163+ Macrophages by R. Clive Landis, Pandelis Philippidis, Jan Domin, Joseph J. Boyle, Dorian O. Haskard

“…In this study, we examined whether scavenging of polymorphic Hp:Hb complexes differentially generated downstream anti-inflammatory signals in cultured human macrophages culminating in interleukin (IL)-10 secretion. …”
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The Novel Methods for Analysis of Exosomes Released from Endothelial Cells and Endothelial Progenitor Cells by Jinju Wang, Runmin Guo, Yi Yang, Bradley Jacobs, Suhong Chen, Ifeanyi Iwuchukwu, Kenneth J. Gaines, Yanfang Chen, Richard Simman, Guiyuan Lv, Keng Wu, Ji C. Bihl

“…Here we described novel methods for purification and phenotyping of EXs released from endothelial cells (ECs) and endothelial progenitor cells (EPCs) by combining microbeads and fluorescence quantum dots (Q-dots®) techniques. EXs from the culture medium of ECs and EPCs were isolated and detected with cell-specific antibody conjugated microbeads and second antibody conjugated Q-dots by using nanoparticle tracking analysis (NTA) system. …”
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RETRACTED: Palmitic Acid Impedes Extravillous Trophoblast Activity by Increasing MRP1 Expression and Function by Yunali Ashar, Qiuxu Teng, John N. D. Wurpel, Zhe-Sheng Chen, Sandra E. Reznik

“…The rate of folate efflux from the cells into the media increased with a decrease in migration and invasion functions in the cultured cells. Treatment with N-acetylcysteine (NAC) prevented the palmitic acid-mediated upregulation of MRP1 and restored invasion and migration in the EVTs. …”
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