Advances, challenges and future applications of liver organoids in experimental regenerative medicine by Da Gong, Jiaye Mo, Jiaye Mo, Mei Zhai, Fulin Zhou, Guocai Wang, Shaohua Ma, Xiaoyong Dai, Xiaoyong Dai, Xuesong Deng

“…Liver organoid technology represents a novel in vitro culture system. After several years of refinement, human liver organoids can now accurately replicate the liver’s morphological structure, nutrient and drug metabolism, gene expression, and secretory functions, providing a robust model for liver disease research. …”
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Current practices and key challenges associated with the adoption of resilient, circular, and sustainable food supply chain for smallholder farmers to mitigate food loss by Jessika Milagros Vásquez Neyra, Jessika Milagros Vásquez Neyra, Mirza Marvel Cequea, Valentina Gomes Haensel Schmitt

“…Key challenges include technological limitations, restricted market access, and low consumer awareness.DiscussionA hybrid model is proposed, combining resource-efficient technologies with culturally rooted practices, fostering multi-stakeholder collaboration. …”
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Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Health Care Workers in Tertiary and Regional Hospitals in Dar es Salam, Tanzania by Agricola Joachim, Sabrina J. Moyo, Lillian Nkinda, Mtebe Majigo, Sima Rugarabamu, Elizabeth G. Mkashabani, Elia J. Mmbaga, Naboth Mbembati, Said Aboud, Eligius F. Lyamuya

“…Nasal swabs were collected from HCWs and cultured on mannitol salt agar. S. aureus was identified based on colonial morphology, Gram staining, catalase, coagulase, and DNase test results. …”
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Identification and characterization of antibacterial peptides produced by Lactobacillus plantarum 1407 by Silpa Sajan, Rupachandra Saravanan

“…Results: Antibacterial activity and mode of action of peptides isolated from L. plantarum 1407 against gram-positive and gram-negative bacteria have been studied. L. plantarum culture exhibited maximum antibacterial activity at 40 °C, pH 8, and 0.7% salt concentration. …”
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Intracellular Ca2+ Stores and Ca2+ Influx Are Both Required for BDNF to Rapidly Increase Quantal Vesicular Transmitter Release by Michelle D. Amaral, Lucas Pozzo-Miller

“…Furthermore, labeling presynaptic terminals with amphipathic styryl dyes and then monitoring their post-BDNF destaining in slice cultures by multiphoton excitation microscopy revealed that the increase in frequency of mEPSCs reflects vesicular fusion events. …”
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Tuberculosis Mimicking-Musculoskeletal Tumor of The Hand: An Uncommon Case of Extrapulmonary Tuberculosis by Andy Lesmana, Hapsari Retno Dewanti, Lenty BR Perangin Angin, Muhamad Irvan Muzakky, Stefi Geovani Valentin Hayon, Mentari Maratus Sholihah, Muhammad Ifham Hanif

“…Histopathological examination and culture are needed to confirm the diagnosis. Conclusions: Slow progression and multiple differential diagnoses frequently result in a delayed diagnosis, which leads to complications. …”
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Clostridium difficile Peritonitis: An Emerging Infection in Peritoneal Dialysis Patients by Saira Chaughtai, Bhavika Gandhi, Zeeshan Chaughtai, Dana Tarina, Mohammad A. Hossain, Arif Asif

“…While the peritoneal fluid culture did not grow the organism (possibly because of prior empiric broad-spectrum antibiotics use), the positive PCR on stool analysis suggested C. difficile-related peritonitis, along with the rapid clinical improvement induced by C. difficile-directed therapy (metronidazole) and discontinuation of broad-spectrum antibiotics. …”
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Relaxin inhibits 177Lu-EDTMP associated cell death in osteosarcoma cells through notch-1 pathway by Xu Junhua, Wan Song, Chen Wei, Zhang Yi, Ji Zhenzhong

“…MG63 and Saos-2 cells were cultured with 177Lu-EDTMP (37 MBq) for 24 h with and without pretreatment of recombinant relaxin 2 (RLXH2) for 12 and 24 h. 177Lu-EDTMP associated cellular deterioration and death was determined by LDH, MTT, and trypan blue dye assays. …”
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Exploratory Factor Analysis on the Talent Development Environment Questionnaire (TDEQ-5) for Basketball in Indonesia by Kukuh Hardopo Putro, Suharjana Suharjana, Agus Susworo Dwi Marhaendro, Fauzi Fauzi, Danang Wicaksono, Subagyo Irianto, Trisnar Adi Prabowo

“…However, further development of this instrument is recommended to be adapted to the sports culture in Indonesia and to ensure a more comprehensive and scientific evaluation of talent coaching in basketball. …”
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Protein C deficiency with recurrent systemic thrombosis associated with compound heterozygous PROC missense variants by Mikio Shiba, Shuichiro Higo, Yu Morishita, Yasuhiro Ichibori, Yoshihiro Kin, Yasushi Sakata, Yoshiharu Higuchi

“…Western blot analysis of Huh-7 and HEK293T cells transfected with expression vectors encoding PROC with or without these variants demonstrated that Gly334Ser-PROC expression levels were significantly decreased in culture media collected from HEK293T cells, while the expression levels of protein C with these variants were not significantly altered in cell lysates. …”
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Saprotrophic Wood Decay Ability and Plant Cell Wall Degrading Enzyme System of the White Rot Fungus <i>Crucibulum laeve</i>: Secretome, Metabolome and Genome Investigations by Alexander V. Shabaev, Olga S. Savinova, Konstantin V. Moiseenko, Olga A. Glazunova, Tatyana V. Fedorova

“…Metabolomic analysis revealed that, specifically, monosaccharides and carboxylic acids were the key low molecular metabolites in the <i>C. laeve</i> culture liquids in the experimental conditions. The proportion of monosaccharides and polyols in the total pool of identified compounds increased on the sawdust-containing media. …”
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Upregulation of CD19 by low‐dose chidamide promotes CAR T cells functionality in B-cell non-Hodgkin lymphoma by Ying Ni, Qun Zhang, Xiaochen Tang, Xiuchun Li, Shiguang Ye, Yan Lu, Aibin Liang, Ping Li

“…CD19 CAR T cells secreted more cytokines (IL-2, TNF-α, and IFN-γ) after co-culture with B-NHL cells pretreated with chidamide. …”
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Selective Immunomodulation of Inflammatory Pathways in Keratinocytes by the Janus Kinase (JAK) Inhibitor Tofacitinib: Implications for the Employment of JAK-Targeting Drugs in Psor... by Martina Morelli, Claudia Scarponi, Laura Mercurio, Francesco Facchiano, Sabatino Pallotta, Stefania Madonna, Giampiero Girolomoni, Cristina Albanesi

“…We demonstrated the selectivity of tofacitinib inhibitory action on IFN-γ and IL-22, but not on TNF-γ or IL-17 proinflammatory signaling, with suppressed expression of IFN-γ-dependent inflammatory genes, and restoration of normal proliferative and differentiative programs altered by IL-22 in psoriatic keratinocyte cultures. Tofacitinib also potently reduced the psoriasiform phenotype in the imiquimod-induced murine model of psoriasis. …”
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Therapeutic Potential of Stem Cells in Follicle Regeneration by Agnieszka Owczarczyk-Saczonek, Magdalena Krajewska-Włodarczyk, Anna Kruszewska, Łukasz Banasiak, Waldemar Placek, Wojciech Maksymowicz, Joanna Wojtkiewicz

“…We know that stem cells can be used to regenerate hair in several therapeutic strategies: reversing the pathological mechanisms which contribute to hair loss, regeneration of complete hair follicles from their parts, and neogenesis of hair follicles from a stem cell culture with isolated cells or tissue engineering. …”
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Prioritizing implementation solutions for the urban family physician policy in Iran: a multi-criteria decision-making study by Lida Shams, Mohammadreza Mobinizadeh, Taha Nasiri, Fatemeh Mohammadi

“…Results Main themes were identified, including financing, management, human resources, structure, culture building, payment mechanism information systems, monitoring & control, performance of insurance organisations, and implementation. …”
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Sustained Interleukin-1β Exposure Modulates Multiple Steps in Glucocorticoid Receptor Signaling, Promoting Split-Resistance to the Transactivation of Prominent Anti-Inflammatory Ge... by Pedro Escoll, Ismael Ranz, Norman Muñoz-Antón, Ana van-den-Rym, Melchor Alvarez-Mon, Carlos Martínez-Alonso, Eva Sanz, Antonio de-la-Hera

“…As sustained IL-1β exposure is key in the pathogenesis of several major diseases with prevalent GCR, we examined GR signaling and the mRNA expression of six GR-driven genes in cells cultured in IL-1β and afterwards challenged with GC. …”
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Sulforaphane attenuates dextran sodium sulphate induced intestinal inflammation via IL-10/STAT3 signaling mediated macrophage phenotype switching by Yuyang Sun, Jiqing Tang, Cui Li, Jun Liu, Haijie Liu

“…This resulted in alteration of inflammatory mediators, particularly interleukin-10 (IL-10), both in colon tissues and culture medium of BMDMs. Subsequently, IL-10 was found to mediate the sulforaphane induced M2 phenotype switching of BMDMs through the activation of STAT3 signaling. …”
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Assessing the Impact of Point-based License Systems on Road Safety: A Systematic Review and Meta-analysis by Francisco Alonso, Mireia Faus, Cristina Esteban, Bosco Martí

“…Conclusions: The findings support the implementation and expansion of the point-based license system in more countries around the world as a valuable strategy to reduce traffic accidents and promote a safer driving culture.…”
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Comparison of CRISPR-Cas13b RNA base editing approaches for USH2A-associated inherited retinal degeneration by Lewis E. Fry, Lauren Major, Ahmed Salman, Lucy A. McDermott, Jun Yang, Andrew J. King, Michelle E. McClements, Robert E. MacLaren

“…We compare the Cas13 effectors PspCas13b and Cas13bt3 for the repair of the gene USH2A, a common cause of inherited retinal disease and Usher syndrome. In cultured cells, we demonstrate up to 80% efficiency for the repair of the common c.11864 G > A and its murine equivalent c.11840 G > A, across different gRNAs and promoters. …”
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Effect of opercular deformity on morphological and transcriptomic profiling of gills in golden pompano (Trachinotus ovatus) by Yuwei Wu, Qian Ma, Dihao Huang, Liuyong Wang, Qiling Zhou, Chunli Ran, Gang Chen

“…The golden pompano (Trachinotus ovatus), one of China's most important economic fishes in marine culture, is highly susceptible to external environmental changes during early development. …”
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