Tumor organoids in cancer medicine: from model systems to natural compound screening by Rong Cong, Can Lu, Xinying Li, Zhijie Xu, Yaqin Wang, Shusen Sun

“…Context The advent of tissue engineering and biomedical techniques has significantly advanced the development of three-dimensional (3D) cell culture systems, particularly tumor organoids. These self-assembled 3D cell clusters closely replicate the histopathological, genetic, and phenotypic characteristics of primary tissues, making them invaluable tools in cancer research and drug screening.Objective This review addresses the challenges in developing in vitro models that accurately reflect tumor heterogeneity and explores the application of tumor organoids in cancer research, with a specific focus on the screening of natural products for antitumor therapies.Methods This review synthesizes information from major databases, including Chemical Abstracts, Medicinal and Aromatic Plants Abstracts, ScienceDirect, Google Scholar, Scopus, PubMed and Springer Link. …”
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Impact of heteroresistance on treatment outcomes of people with drug-resistant TB by R. Crowder, M. Kato-Maeda, B. Schwem, A. dela Tonga, D.M. Geocaniga-Gaviola, E. Lopez, C.L. Valdez, A.R. Lim, N. Hunat, A.G. Sedusta, C.A. Sacopon, G.A.M. Atienza, E. Bulag, D. Lim, J. Bascuña, K. Shah, R.P. Basillio, C.A. Berger, M.C.D.P. Lopez, S. Sen, C. Allender, M. Folkerts, U. Karaoz, E. Brodie, S. Mitarai, A.M.C. Garfin, M.C. Ama, D.M. Engelthaler, A. Cattamanchi, R. Destura

“…METHODS: We used targeted deep sequencing to identify mutations associated with DR-TB and heteroresistance in culture samples of 624 people with DR-TB. We evaluated the association between heteroresistance and time to unfavorable treatment outcome using Cox proportional hazards regression. …”
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Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis by Shoukui He, Beining Ye, Zengfeng Zhang, Yan Cui, Siyun Wang, Xianming Shi

“…Moreover, exogenous addition of C16:0 fatty acid to stationary-phase acid-adapted cultures was able to enhance bacterial ethanol tolerance. …”
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Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc by Liangjie Liu, Ya Shu, Tian Liu, Huilan Wei, Yaxin Yang, Lijing Zhang, Xiaohui Ma, Guoqing Li, Yajuan Li, Shi Wang, Lingling Zhang

“…This approach will facilitate the culture and manipulation of spermatogonia in non-model organisms, which may contribute to genetic improvement in aquaculture.…”
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Social Justice Work in the University: Understanding Student and Staff Perceptions and Aspirations for Decolonising the Curriculum from a University-Wide Survey by Kyra Araneta, Kelsea Costin, Jennifer Fraser, Fatima Maatwk, Özge Süvari, Esra Tahir

“…Positioned within the university’s broader commitments to anti-racism and as part of a student–staff partnership project focused on interrogating contemporary coloniality, this research investigates how participants understand and experience decolonial initiatives as well as the perceived impact of these efforts on curriculum, relationships, and institutional culture. By conducting a survey, we sought to unravel the complexities surrounding how students and staff conceptualise decolonisation, articulate their aspirations for decolonial initiatives, and envision the potential of student–staff partnerships as catalysts for transformative social justice work within the university. …”
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RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells by Bruno Hernáez, Graciela Alonso, Juan Manuel Alonso-Lobo, Alberto Rastrojo, Cornelius Fischer, Sascha Sauer, Begoña Aguado, Antonio Alcamí

“…Addition of UV-inactivated virus particles to cell cultures altered the expression of a set of 53 cellular genes, including genes involved in innate immunity. …”
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Exploring Customary Law: Perspectives of Hazairin and Cornelis Van Vollenhoven and its Relevance to the Future of Islamic Law in Indonesia by Ilham Tohari, Siti Rohmah, Ahmad Qiram As-Suvi

“…The formation of national law due to culture and tradition is an important part of society. …”
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Complex and severe infection in a 67-year-old liver transplant recipient due to Cunninghamella elegans, Bordetella bronchiseptica, and Pneumocystis jirovecii by Muyun Wei, Kangyi Gu, Juanxiu Qin, Xinhua Lu, Junqi Feng, Shaowei Mao, Min Li, Zhilan Li, Changsheng Wang

“…Subsequent mNGS testing further revealed the presence of C. elegans, indicating that different pathogens played dominant roles at various stages of the disease progression. The routine culture also identified several of the above pathogens, but the results were reported much later than those of mNGS. …”
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Efficacy of human umbilical cord mesenchymal stem cell in the treatment of neuromyelitis optica spectrum disorders: an animal study by Chunran Xue, Haojun Yu, Xuzhong Pei, Xiaoying Yao, Jie Ding, Xiying Wang, Yi Chen, Yangtai Guan

“…Furthermore, in vitro studies revealed that co-culture with hUC-MSCs significantly restored astrocyte viability and reduced apoptosis in AQP4 IgG and complement-mediated damage. …”
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Human Cardiac Fibroblast Number and Activation State Modulate Electromechanical Function of hiPSC-Cardiomyocytes in Engineered Myocardium by Cassady E. Rupert, Tae Yun Kim, Bum-Rak Choi, Kareen L. K. Coulombe

“…Second, hCFs undergo myofibroblast activation in traditional two-dimensional culture, and this altered phenotype ablated the functional benefits of hCFs when incorporated into engineered cardiac tissues. …”
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Risk Factors for Mortality in Melioidosis: A Single-Centre, 10-Year Retrospective Cohort Study by Raviraj Menon, Poornima Baby, Anil Kumar V., Sandeep Surendran, Manu Pradeep, Aiswarya Rajendran, Gaayathri Suju, Arathy Ashok

“…Clinical, demographic, and biochemical data in patients diagnosed with melioidosis with cultures were collected between January 2011 and December 2020 from medical records. …”
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Optimization of Acid Protease Production by Aspergillus niger I1 on Shrimp Peptone Using Statistical Experimental Design by Rayda Siala, Fakher Frikha, Samiha Mhamdi, Moncef Nasri, Alya Sellami Kamoun

“…Medium composition and culture conditions for the acid protease production by Aspergillus niger I1 were optimized by response surface methodology (RSM). …”
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Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line by Nafi’u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris

“…It appears that a single mutation of UPM190 and UPM0081 IBDV isolates at D279N could facilitate vvIBDV strain adaptability in CEE and BGM-70 cultures.…”
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Architecture Competitions as an Opportunity for Young Women Architects. The Case of the Competition for Rural Subsidised Housing and the Canarian Magüi González, 1981-85 by David Delgado Baudet, Lucía C. Pérez-Moreno

“…Finally, the investigation focuses, from a compositional, constructive and design culture perspective, on González’s final built project: 30 dwellings in Juan Grande in Las Palmas de Gran Canaria (1983-85), which was, indeed, her opera prima and was eventually awarded an important regional architecture prize.…”
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MicroRNA-208a Dysregulates Apoptosis Genes Expression and Promotes Cardiomyocyte Apoptosis during Ischemia and Its Silencing Improves Cardiac Function after Myocardial Infarction by Hasahya Tony, Kai Meng, Bangwei Wu, Aijia Yu, Qiutang Zeng, Kunwu Yu, Yucheng Zhong

“…The effect of miR-208a on apoptosis during ischemia was studied in cultured neonatal mice myocytes transfected with agomir or antagomir. …”
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Prospects of Using Microbiota Correction Methods in the Treatment of Inflammatory Bowel Disease by A. I. Akinshina, D. V. Smirnova, A. V. Zagainova, V. V. Makarov, S. M. Yudin

“…In the past, bacteriological methods based on the isolation of a pure bacterial culture were used to determine the microbiota composition. …”
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Rapid life-history evolution reinforces competitive asymmetry between invasive and resident species by Chapuis, Elodie, Jarne, Philippe, David, Patrice

“…We here observe negative impacts of competition by both species on each other, though P. acuta is dominant and over generations largely displaces A. marmorata from co-cultures. In addition, our experiments suggest that A. marmorata populations having experienced competition by P. acuta for sufficient time in nature, have evolved to become even less tolerant to it. …”
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CXC chemokine receptor 7 ameliorates renal fibrosis by inhibiting β-catenin signaling and epithelial-to-mesenchymal transition in tubular epithelial cells by Ping Meng, Chunli Liu, Jingchun Li, Ping Fang, Bo Yang, Wei Sun, Yunfang Zhang

“…Administration of pFlag-CXCR7, an overexpression plasmid for CXCR7, significantly inhibited the activation of β-catenin signaling and protected against the progression of epithelial-to-mesenchymal transition (EMT) and renal fibrosis in a UIRI mouse model. Using cultured HKC-8 cells, we found that CXCR7 significantly downregulated the expression of active β-catenin and fibrosis-related markers, including fibronectin, Collagen I, and α-SMA. …”
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Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines by Elizabeth Curtiss, Youde Jiang, Li Liu, Claire Hawthorne, Jessica Zhang, Jena J. Steinle

“…Primary human REC were cultured in normal and high glucose followed by Epac1 agonist treatment or transfection with IL-1β or TNFα siRNA for protein analyses of insulin signaling proteins. …”
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Ideologization and commercialization in pandemic management by Anđelković Maja, Radosavljević Milan, Radosavljević Dragana

“…However, the crisis of the pandemic is more relevant by nature, because it is about people's lives and health, globally, encompassing all countries, different nations, cultures, religions, but also different gender, age, education and other structures. …”
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