Development and validation of the Iranian Social Health Questionnaire (IrSHQ) by Hassan Rafiey, Mostafa Amini Rarani, Fardin Alipour, Esmaeil Khedmati Morasae

“…Conclusion: Due to the size and diversity of participants, validity of results, compliance with Iranian culture, and its relative shortness, the IrSHQ appears to be a very useful instrument for measuring individual’s social health in the Iranian social context.…”
Get full text

Oct4 Gene Expression in Primary Colorectal Cancer Promotes Liver Metastasis by Shiki Fujino, Norikatsu Miyoshi

“…The capacity to form liver metastasis in vivo was examined using CRC cell lines and primary cultured CRC cells. Results. LMFS was significantly poor in the Oct4 high-expression group compared with the low-expression group (P=0.008). …”
Get full text

The Influence of Ouabain on Human Dendritic Cells Maturation by C. R. Nascimento, R. C. Valente, J. Echevarria-Lima, C. F. L. Fontes, L. de Araujo-Martins, E. G. Araujo, V. M. Rumjanek

“…To generate immature DCs, human monocytes were cultured with IL-4 and GM-CSF (5 days). To investigate Ouabain role on DC activation, DCs were stimulated with TNF-α for 48 h in the presence or absence of Ouabain. …”
Get full text

Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs by Anna Schade, Paula Müller, Evgenya Delyagina, Natalia Voronina, Anna Skorska, Cornelia Lux, Gustav Steinhoff, Robert David

“…However, most of the studies use cultured hMSCs, although these can lose their stem cell characteristics during expansion. …”
Get full text

Epithelial-to-Mesenchymal Transition in Pancreatic Ductal Adenocarcinoma and Pancreatic Tumor Cell Lines: The Role of Neutrophils and Neutrophil-Derived Elastase by Thomas Große-Steffen, Thomas Giese, Nathalia Giese, Thomas Longerich, Peter Schirmacher, G. Maria Hänsch, Matthias M. Gaida

“…EMT was also induced when the tumor cells were grown under conditions preventing attachment to the culture plates. Here, also in the absence of elastase, E-cadherin was downmodulated. …”
Get full text

Biological Character of RetroNectin Activated Cytokine-Induced Killer Cells by Lu Han, Yi-Man Shang, Yong-Ping Song, Quan-Li Gao

“…Moreover, this ability continued after IL-2 was withdrawn from the culture system. R-CIK cells could also secrete higher levels of IL-2 and lower levels of IL-4 and IL-5 versus OKT-CIK cells. …”
Get full text

Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater by Qian Yang, Wenzhuan Huang, Xue Yan, Qiang Ding, Jiaxin Liu, Bo Cheng, Tao Duan

“…In this study, Cupriavidus basilensis and Bacillus velezensis were isolated from pharmaceutical wastewater and neomycin-free mangrove soil through enrichment culture and gradual acclimatization, respectively. …”
Get full text

Recovery of Phenolic Compounds with Antioxidant Capacity Through Solid-State Fermentation of Pistachio Green Hull by Andrés Javier Ordoñez-Cano, Ulises Ramírez-Esparza, Fernando Méndez-González, Mónica Alvarado-González, Ramiro Baeza-Jiménez, Leonardo Sepúlveda-Torre, Lilia Arely Prado-Barragán, José Juan Buenrostro-Figueroa

“…<i>A. niger</i> GH1 was able to grow and colonize the PGH, with the higher value of TPC (23.83 mg/g of dry mass (gdm)) obtained after 24 h of culture, which significantly correlated with AC (Pearson’s R = 0.69). …”
Get full text

A unique case report on campylobacter rectus infection leading to acute motor axonal neuropathy in a pediatric patient by Uchechukwu Ibewuike, MBBS, John Kim, MD, Swati Mody, MBBS, Gregory Obala, MD, Ebere Ibezim, MBBS

“…A punch biopsy of the left periorbital soft tissue yielded a culture positive for Campylobacter rectus. Following this, the patient developed weakness in all 4 limbs, with more pronounced weakness in the legs than in the arms, and he lost motor function in both lower legs, although sensory function remained intact. …”
Get full text

Role of Peroxisome Proliferator-Activated Receptor β/δ and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells by Jeffrey D. Coleman, Jerry T. Thompson, Russell W. Smith, Bogdan Prokopczyk, John P. Vanden Heuvel

“…PPARβ/δ activation reduced the TNFα-induced expression of various genes implicated in metastasis and reduced the invasion through a basement membrane in cell culture models. Through the use of short hairpin RNA inhibitors of PPARβ/δ, BCL-6, and MMP-9, it was evident that PPARβ/δ was responsible for the ligand-dependent effects whereas BCL-6 dissociation upon GW501516 treatment was ultimately responsible for decreasing MMP-9 expression and hence invasion activity. …”
Get full text

A Prospective Study of Tuberculosis Drug Susceptibility in Sabah, Malaysia, and an Algorithm for Management of Isoniazid Resistance by Muhammad Redzwan S. Rashid Ali, Uma Parameswaran, Timothy William, Elspeth Bird, Christopher S. Wilkes, Wai Khew Lee, Tsin Wen Yeo, Nicholas M. Anstey, Anna P. Ralph

“…Results. 176 outpatients were enrolled; 173 provided sputum samples. Mycobacterial culture yielded M. tuberculosis in 159 (91.9%) and nontuberculous Mycobacterium (NTM) in three (1.7%). …”
Get full text

Neurohypophysial Receptor Gene Expression by Thymic T Cell Subsets and Thymic T Cell Lymphoma Cell Lines by I. Hansenne, G. Rasier, Ch. Charlet-renard, M.-P. Defresne, R. Greimers, C. Breton, J.-J. Legros, V. Geenen, H. Martens

“…In murine fetal thymic organ cultures, a specific OTR antagonist does not modify the percentage of T cell subsets, but increases late T cell apoptosis further evidencing the involvement of OT/OTR signaling in the control of T cell proliferation and survival. …”
Get full text

Dual Functions of the C5a Receptor as a Connector for the K562 Erythroblast-Like Cell-THP-1 Macrophage-Like Cell Island and as a Sensor for the Differentiation of the K562 Erythrob... by Hiroshi Nishiura, Rui Zhao, Tetsuro Yamamoto

“…In this study, we detected C5aR expression and RP S19 oligomer generation in human erythroleukemia K562 cells during haemin-induced erythropoiesis. Under monocell culture conditions, the differentiation into K562 erythrocyte-like cells was enhanced following the overexpression of Wild-type RP S19. …”
Get full text

Synergistic Approach of High-Precision 3D Printing and Low Cell Adhesion for Enhanced Self-Assembled Spheroid Formation by Chunxiang Lu, Aoxiang Jin, Chuang Gao, Hao Qiao, Huazhen Liu, Yi Zhang, Wenbin Sun, Shih-Mo Yang, Yuanyuan Liu

“…Compared to traditional methods, our proposed strategy enables high-throughput production of size-controlled spheroids with excellent shape retention, while enhanced gas exchange during culture improves differentiation marker expression. …”
Get full text

Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions by Fei Li, Fei Li, Ming-Ben Xu, Liang-Hao Pan, Jie Li, Cai-Bi Lan, Zhe Li, Shan Lu, Jun-Xiang Lai, Jun-Xiang Lai, Peng-Fu Li

“…Genomic analysis revealed that strain GXAS 306T had many potential functions favorable for interacting with algae, and further experimental evidence confirmed the ammonifying and phosphorus-solubilizing function. Co-culture experiments showed that strain GXAS 306T significantly improved algal growth parameters of two typical P. globosa strains (Pg293 and PgV01), particularly under nitrogen or phosphorus deficiency. …”
Get full text

Pre-pregnancy obesity and weight gain during pregnancy: impact on newborn outcomes by Sezgin Gunes, Suzan Sahin, Meltem Koyuncu Arslan, Nurhan Korkmaz, Ozlem Karaca Dag, Emir Gokalp, Hazal Saban, Mehmet Koca, Mehmet Yekta Oncel

“…High obesity rates and ethnic disparities highlight the need for culturally tailored prenatal care to improve maternal and neonatal health, especially in immigrant populations. …”
Get full text

PPARα: A Master Regulator of Bilirubin Homeostasis by Cyril Bigo, Jenny Kaeding, Diala El Husseini, Iwona Rudkowska, Mélanie Verreault, Marie Claude Vohl, Olivier Barbier

“…Human umbilical vein epithelial cells (HUVEC) and coronary artery smooth muscle cells (CASMC) were cultured in the absence or presence of the 3 activators, and mRNA, protein, and/or activity levels of the bilirubin synthesizing heme oxygenase- (HO-) 1 and biliverdin reductase (BVR) enzymes were determined. …”
Get full text

The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages by Mariana Oliveira Arruda, Saulo José Figueiredo Mendes, Simone Aparecida Teixeira, Ludmilla Santos Silva de Mesquita, Maria Nilce de Sousa Ribeiro, Stanley de Sousa Lima Galvão, Marcelo Nicolás Muscará, Elizabeth Soares Fernandes, Valério Monteiro-Neto

“…We evaluated the effects of the peppermint leaf hydroalcoholic extract (LHAE) on cultured murine peritoneal macrophages stimulated or not with lipopolysaccharide (LPS) in vitro. …”
Get full text

Leclercia adecarboxylata Cholecystitis with Septic Shock in Immunocompetent Patient by Nooraldin Merza, John Lung, Ahmed Taha, Ahmed Qasim, Jill Frost, Tarek Naguib

“…An ultrasound guided gallbladder drain was performed. Culture of the biliary fluid yielded pure growth of pan-sensitive L. adecarboxylata; antibiotics were narrowed accordingly. …”
Get full text

Infection and HLA-G Molecules in Nasal Polyposis by Roberta Rizzo, Nicola Malagutti, Daria Bortolotti, Valentina Gentili, Antonella Rotola, Enrico Fainardi, Teresa Pezzolo, Claudia Aimoni, Stefano Pelucchi, Dario Di Luca, Antonio Pastore

“…HPV-11 positive SNP-WoAD patients presented with mHLA-G and IL-10R on epithelial cells from nasal polyps and showed secretion of sHLA-G and IL-10 in culture supernatants. No HLA-G expression was observed in HPV negative polyps. …”
Get full text