Rapid isolation and recovery of Salmonella using hollow glass microspheres coated with multilayered nanofilms by Rutwik Joshi, Hesaneh Ahmadi, Md Nayeem Hasan Kashem, Fariha Afnan, Siva Parameswaran, Chau-Chyun Chen, Gizem Levent, Wei Li

“…Timely isolation, recovery, and identification of Salmonella from food samples is essential for prevention and control of foodborne Salmonella outbreaks. Traditional culture-based Salmonella isolation and serotyping techniques are time consuming and labor intensive. …”
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Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model by Atsushi Mima, Azuma Kimura, Ryo Ito, Yu Hatano, Hiraku Tsujimoto, Shin-Ichi Mae, Junko Yamane, Wataru Fujibuchi, Norimitsu Uza, Taro Toyoda, Hiroshi Seno, Kenji Osafune

“…Furthermore, differential gene expression analysis revealed upregulated pathways, including cAMP-related signals, involved in the differentiation of hiPSC-derived pancreatic acinar lineage cells in vivo, and we found that a cAMP activator, forskolin, facilitates the differentiation from hiPSC-derived pancreatic endoderm into pancreatic acinar progenitor cells in our in vitro differentiation culture. Therefore, this platform contributes to our understanding of the developmental mechanisms of pancreatic acinar lineage cells and the establishment of differentiation methods for acinar cells from hiPSCs.…”
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Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava by Dubravka Štajner, Boris M. Popović, Dušica Ćalić, Marijana Štajner

“…The leaves of A. flava collected from the botanical garden exhibited stronger antioxidant activity (higher activities of SOD, and higher quantities of GSH, TSH, TPC, and scavenging abilities of DPPH and NO•, and higher FRAP values and lowest quantities of •OH and MDA) than in vitro obtained cultures. However, the leaves of A. flava showed higher antioxidant activity than the leaves of A. hippocastanum, and therefore they have a stronger tolerance of oxidative stress. …”
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Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδ/c-Src/EGFR/PI3K/Akt/AP-1 Signaling Pathway in Human Chondrocytes by Chun-Yin Huang, Hsiu-Jung Lin, Hsin-Shui Chen, Shi-Yann Cheng, Horng-Chaung Hsu, Chih-Hsin Tang

“…We found that stimulation with thrombin led to increased secretion of MMP-13 in cultured human chondrocytes. Further, this thrombin-induced MMP-13 production was reduced after transfection with siRNAs against protease activated receptors 1 and 3 (PAR1 and PAR3), but not with PAR4 siRNA. …”
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Biofilm Formation among Clinical and Food Isolates of Listeria monocytogenes by Joana Barbosa, Sandra Borges, Ruth Camilo, Rui Magalhães, Vânia Ferreira, Isabel Santos, Joana Silva, Gonçalo Almeida, Paula Teixeira

“…Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were tested for biofilm formation after being exposed to acidic and osmotic stress conditions. …”
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Clinical Evaluation of a Rapid Reciprocal-Flow PCR Assay and Real-Time PCR Assay with Quenching Probe for Detection of <i>Mycobacterium tuberculosis</i> Complex by Kosuke Kosai, Keisuke Matsumoto, Takahisa Ishikawa, Yasuhide Kawamoto, Norihiko Akamatsu, Kenji Ota, Fujiko Mitsumoto-Kaseida, Norihito Kaku, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

“…These assays were performed for stored clinical samples and results were compared with the confirmed results based on culture and COBAS TaqMan MTB assay. A total of 53 samples (20 confirmed positives and 33 confirmed negatives) were included in the performance analysis. …”
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Community Engaged Lifestyle Modification Research: Engaging Diabetic and Prediabetic African American Women in Community-Based Interventions by Starla Hairston Blanks, Henrie Treadwell, Anya Bazzell, Whitney Graves, Olivia Osaji, Juanita Dean, James T. McLawhorn, Jareese Lee Stroud

“…The I Am Woman (IAW) Program is a community-based, culturally responsive, and gender-specific nutrition, obesity, and diabetes educational prevention program designed for African American women (AAW). …”
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Leaders' Degree of Strategic Improvisation Practice from Education Employees' Perspective by Rahmeh Abbaas B. Alhameedyeen, Ola Mahmoud K. Alhamaidah

“…The study recommended that the Ministry of Education adopt an organizational culture that encourages strategic improvisation by emphasizing intuition, creativity, adaptation to change, and risk perception while granting sufficient authority to ministry leaders to practice strategic improvisation in ways that enhance workflow and improve the quality of outcomes. …”
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Randomized Crossover Study Showing Nurse-Led Same Day Review Replacing Next Day Review in Uneventful Phacoemulsification to Be Safe and Efficacious by Jennifer W. H. Shum, Janice J. J. Cheung, Monica M. N. Lee, Oscar G. W. Wong, Kenneth K. W. Li

“…Patient satisfaction is also comparable in the setting of Asian culture and when transportation is not a major concern.…”
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Stress-Induced Premature Senescence or Stress-Induced Senescence-Like Phenotype: One In Vivo Reality, Two Possible Definitions? by Olivier Toussaint, Patrick Dumont, Jose Remacle, Jean-Francois Dierick, Thierry Pascal, Christophe Frippiat, Joao Pedro Magalhaes, Stephanie Zdanov, Florence Chainiaux

“…As stress increases, the proportion of cells in “stress-induced premature senescence-like phenotype” according to definition 1 or “stress-induced premature senescence,” according to definition 2, should increase when a culture reaches growth arrest, and the proportion of cells that reached telomere-dependent replicative senescence due to the end-replication problem should decrease. …”
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Skin and Soft Tissue Infections in Patients with Solid Tumours by Diamantis P. Kofteridis, Antonios Valachis, Eirini Koutsounaki, Sofia Maraki, Eleni Mavrogeni, Foteini N. Economidou, Dimitra Dimopoulou, Kostas Kalbakis, Vassilis Georgoulias, George Samonis

“…Cellulitis/erysipelas was the most common clinical presentation (56; 69%). Bacterial cultures were possible in 29 (36%) patients. All patients received antimicrobial therapy, while in 17 episodes (21%) an incision and drainage was required. …”
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Comparison of Real-Time Methods Demonstrating the Effects of Reduced Glutathione on Olfactory Neuroblasts by Alain Géloën, Emmanuelle Berger

“…Results show the importance of real-time analysis methods, without labelling, in the study of cell responses under culture conditions. The present findings highlight important criteria in the choice of methods, beyond the parameters studied, such as cell preparation time, plate filling time, number of cells studied, friendly use of the devices, and the complexity of data processing.…”
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The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells by Ana O. Pires, Andreia Neves-Carvalho, Nuno Sousa, António J. Salgado

“…For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. …”
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Decarbonizing gas emissions from petrochemical production using microalgae by F. Shevlyakov, A.B. Laptev, O.R. Latypov, D.R. Latypova

“…This study aimed to investigate the absorption of greenhouse gases from isoprene production by the marine microalgae Isochrysis galbana and Tetraselmis suecica, as well as the freshwater microalgae Chlorella vulgaris.METHODS: Microalgae cells were cultured in a bioreactor. The grown microalgae strains and mineralized water were fed to the bioreactor. …”
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P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation by Viorel Simion, Cristina Ana Constantinescu, Daniela Stan, Mariana Deleanu, Monica Madalina Tucureanu, Elena Butoi, Ileana Manduteanu, Maya Simionescu, Manuela Calin

“…We developed and characterized dexamethasone-loaded lipid nanoemulsions directed towards P-selectin (PLN-Dex) and monitored their anti-inflammatory effects in vitro using cultured EC (EA.hy926 cells) and in vivo using a mouse model of acute inflammation [lipopolysaccharides (LPS) intravenously administered in C57BL/6 mice]. …”
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Intra-Bone Marrow Administration of Mesenchymal Stem/Stromal Cells Is a Promising Approach for Treating Osteoporosis by Hideki Agata, Yoshinori Sumita, Tatsuro Hidaka, Mayumi Iwatake, Hideaki Kagami, Izumi Asahina

“…Cells capable of forming bone were isolated from the murine compact bones and expanded in culture. Relatively pure MSCs possessing increased potential for cell proliferation, osteogenic differentiation, and inhibition of osteoclastogenesis were obtained by magnetic-activated cell sorting with the anti-Sca-1 antibody. …”
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The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris by Wei-Kuang Lai, Yang-Chang Wu, Tai-Sheng Yeh, Chun-Ren Hsieh, Yi-Hong Tsai, Chien-Kei Wei, Chi-Ying Li, Ying-Chen Lu, Fang-Rong Chang

“…In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. …”
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Inhibition of <i>Salmonella enterica</i> and Enterohemorrhagic <i>Escherichia coli</i> by Ethanolic Extracts of Pomegranate Peels by Weifan Wu, Kevin Mis Solval, Jinru Chen

“…</i> Cells of the two bacterial cultures (5 log CFU/mL) were treated in tryptic soy broth containing 0, 1, or 2% ethanolic extracts of four pomegranate peels for 5, 10, or 24 h at 25 °C. …”
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Staff Management in Social Security Hospitals in Tehran: Challenges and Solutions by Asra Khalili, Ali Mohammad Mosadegh Rad, SHahram Ghafary, Ebrahim Jaafaripooyan

“…Proposed solutions, classified into five main groups and 29 subgroups, encompassed: enhancing recruitment and hiring processes, fostering a dynamic organizational culture, improving human resource productivity and efficiency, transforming hospitals into learning organizations, providing comprehensive and effective training, and increasing transparency in rules and regulations, particularly in job descriptions. …”
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Exploring the characteristics of kombucha tea fermented with soursop (Annona muricata), Noni (Morinda citrifolia) and pineapple (Ananas comosus) by Azizan Muhammad Adib, Qadir Rahman, Zakaria Mizatul Athila, Mohsin Aliah Zannierah, Meor Hussin Anis Shobirin

“…It was fermented using SCOBY (symbiotic culture of bacteria and yeast) for 14 days at room temperature. …”
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