Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression by Ana Carolina Irioda, Rafael Cassilha, Larissa Zocche, Julio Cesar Francisco, Ricardo Correa Cunha, Priscila Elias Ferreira, Luiz Cesar Guarita-Souza, Reginaldo Justino Ferreira, Bassam Felipe Mogharbel, Venkata Naga Srikanth Garikipati, Daiany Souza, Mirian Perlingeiro Beltrame, Katherine Athayde Teixeira de Carvalho

“…The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2. Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively. …”
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In vitro effects of ascorbic acid on viability and metabolism of patients’ osteosarcoma stem cells by Jovičić Marijana Šimić, Pušić Maja, Antunović Maja, Ledinski Maja, Librenjak Lucija, Kolundžić Robert, Ribičić Tomislav, Trkulja Vladimir, Urlić Inga

“…OS-CSC were isolated using a sphere culture system and identified as stem cells using Hoechst 33342 exclusion assay. …”
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Functional Role of SIL1 in Neurodevelopment and Learning by Shilian Xu, Jia Zhu, Kai Mi, Yan Shen, Xiaomin Zhang

“…The basic expression pattern of SIL1 in tissues and cultured cortical neurons is measured by immunostaining and Western blot. …”
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Marital Resilience factors that enhance Conflict Management in Black African Marriages in South Africa: An Afro-Centric Perspective by Andrew Spaumer, Azwihangwisi Helen Mavhandu-Mudzusi, Segun Emmanuel Adewoye

“…Despite the stressful conditions and difficulties that bring about habitual conflict in many Black African marriages, some couples choose to manage their conflict and remain married, even in the context of the divorce culture. It is important to determine the potential factors that impact marital resilience in the midst of difficulties. …”
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Dose-Dependent Differential Effect of Neurotrophic Factors on In Vitro and In Vivo Regeneration of Motor and Sensory Neurons by Daniel Santos, Francisco Gonzalez-Perez, Xavier Navarro, Jaume del Valle

“…Hence, we have characterized in organotypic cultures of spinal cord and dorsal root ganglia the effect of GDNF, FGF-2, NGF, NT-3, and BDNF at different concentrations on motor and sensory neurite outgrowth. …”
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Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays by Richard D. Shipman, Sean D. Doering, Jack R. Hemsath, Eun Joo Lee, Jennifer E. Grant

“…In both Kirby–Bauer tests and liquid culture growth inhibition assays, phosvitin inhibited M. luteus, a microorganism that thrives in the human mouth, but not the other bacteria tested. …”
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A novel Arabidopsis phyllosphere resident Protomyces species and a re-examination of genus Protomyces based on genome sequence data by Kai Wang, Timo Sipilä, Kirk Overmyer

“…Of the 82 named Protomyces, we found few recent studies and six available cultures. We previously isolated Protomyces strains from wild Arabidopsis thaliana, a member of Brassicaceae, a family distant from accepted Protomyces hosts. …”
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The Example of Mersin in the Sustainability of Coastal Cities under the Impact of Climate Change by Nihal Zengin, Ruşen Yamaçlı

“…It is seen that cities, which have hosted different cultures and civilizations in the historical process and have a lively and dynamic structure, have been shaped with different characteristics under the influence of many factors. …”
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Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis by Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Ouyang Lizhi, Parthasarathy Ranganathan, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

“…The cellulolytic activity of tsu1 was detected using carboxymethylcellulose (CMC) plate Congo red assay and the shift towards low-molecular size forms of CMC revealed by gel permeation chromatography in CMC liquid culture and the identification of a cellulase in the secreted proteome.…”
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Tinea manuum: a 5 year retrospective study of demographic data, clinical characteristics, and treatment outcomes by Panittra Suphatsathienkul, Pattriya Jirawattanadon, Lalita Matthapan, Waranyoo Prasong, Chatisa Panyawong, Akkarapong Plengpanich, Sumanas Bunyaratavej, Charussri Leeyaphan

“…Concurrent fingernail onychomycosis was observed in 43%, and 59.8% had additional fungal skin infections. Among 50 positive cultures, dermatophytes accounted for 86% and nondermatophytes for 14%, with Trichophyton rubrum (54%) and Trichophyton mentagrophytes complex (24%) being the most frequent pathogens. …”
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In Vitro Activities of Ertapenem and Imipenem against Clinical Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae Collected in Military Teaching Hospital Mohammed V of R... by M. Elouennass, A. Zohoun, A. El Ameri, N. Alem, J. Kasouati, Y. Benlahlou, I. El Yaagoubi, M. Frikh, A. Lemnouer, A. Benouda

“…Isolates obtained from blood cultures, superficial and deep pus, and catheters were conducted. …”
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Curcumin Protects Neonatal Rat Cardiomyocytes against High Glucose-Induced Apoptosis via PI3K/Akt Signalling Pathway by Wei Yu, Wenliang Zha, Zhiqiang Ke, Qing Min, Cairong Li, Huirong Sun, Chao Liu

“…The function of curcumin on NADPH oxidase-related ROS production and cardiac apoptosis, together with the modulation of protein signalling pathways, was investigated in cardiomyocytes. Primary cultures of neonatal rat cardiomyocytes were exposed to 30 mmol/L high glucose with or without curcumin. …”
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A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4 by Yi-Reng Lin, Chao-Jen Kuo, Hugo You-Hsien Lin, Chin-Jen Wu, Shih-Shin Liang

“…We synthesized unmodified Fe3O4 nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe3O4 NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. …”
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Peroxisome Proliferator-Activated Receptor α Reduces Endothelin-1-Caused Cardiomyocyte Hypertrophy by Inhibiting Nuclear Factor-κB and Adiponectin by Hsu-Lung Jen, Po-Len Liu, Yung-Hsiang Chen, Wei-Hsian Yin, Jaw-Wen Chen, Shing-Jong Lin

“…Human cardiomyocytes (HCMs) were cultured with or without ET-1, whereafter the inhibitory effects of fenofibrate, a PPARα activator, on cell size and adiponectin protein were tested. …”
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Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris by Takuya Ogawa, Shunichi Yokota, Liyile Chen, Yuki Ogawa, Yoshio Nishida, Taiki Tokuhiro, Hend Alhasan, Tomoyo Yutani, Tomohiro Shimizu, Daisuke Takahashi, Takuji Miyazaki, Tsutomu Endo, Ken Kadoya, Mohamad Alaa Terkawi, Norimasa Iwasaki

“…<b>Methods:</b> Inverted cell culture model was used for stimulating the cells with wear debris in vitro, and calvarial osteolysis model was used for evaluating the therapeutic effects of inhibitors in vivo. …”
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Degraded products generated by iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1 by Jiabing Huang, Bingjian Liu, Chunguang Zhao, Jing Li, Dongxu Qiu

“…However, this observation needs to be validated through in vitro experimentation. In this study, co-culture experiments and flow cytometer assays were performed to qualitatively investigate the effects of iron stent degradation on VSMCs. …”
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Zinc regulates microglial polarization and inflammation through IKBα after spinal cord injury and promotes neuronal repair and motor function recovery in mice by Daoyong Li, Daoyong Li, Mingyu Bai, Mingyu Bai, Zhanpeng Guo, Yang Cui, Yang Cui, Xifan Mei, Xifan Mei, He Tian, He Tian, Zhaoliang Shen

“…IKBα expression was evaluated as a potential target of zinc ions, both in cultured cells and in vivo.ResultsTranscriptomic analysis revealed that zinc ions modulate inflammatory pathways through IKBα, which inhibits NF-κB activity. …”
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Development of Entrepreneurial Activity Using the Integration of Human Capital and Green Technologies to Optimize the Sustainable Development of the Territories by Alma Karshalova, Aidos Akpanov, Santay Tleubayeva, Anuar Belgibayev, Askar Makhmudov, Aigul Atchabarova, Margarita Zholayeva

“…The future demand for tourism is likely to be driven by growing environmental awareness, increased use of digital services and new technologies, a shift towards a more personalized travel experience, and ensuring well-being and better engagement with local communities and culture. Meeting the requirements of the modern tourism industry is a problem not only for the Republic of Kazakhstan but also for other countries that strive for sustainable development of the tourism sector. …”
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Implementation of sustainable healthy diet in urban household consumption by Dhyani Swamilaksita Prita, Farida Baliwati Yayuk, Martianto Drajat, Briawan Dodik, Ahzaini Laili Nur

“…A sustainable healthy diet is one that is nutritionally adequate and healthy, safe, culturally acceptable, economically fair and affordable, and has little environmental impact. …”
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<i>Trachemys scripta</i> Eggs as Part of a Potential In Vivo Model for Studying Sea Turtle Egg Fusariosis by María Martínez-Ríos, Laura Martín-Torrijos, Javier Diéguez-Uribeondo

“…., high disease incidence, severity, and low hatching success) and its ability to modify the pH in both the egg surface and culture media, confirming previously described fungal pathogen models. …”
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